Dr. Yi Xiao acquired her Ph.D. in Chemistry from Nanjing University (China) in 2000 under the supervision of Professor Hongyuan Chen. In 2001, she began her first post-doctoral appointment in Professor Itamar Willner's group at The Hebrew University of Jerusalem (Israel) developing optical and electrochemical biosensors and bioelectronics. In 2004, she started her second post-doctoral appointment at the University of California at Santa Barbara (UCSB), working with Nobel Laureate Professor Alan Heeger and Professor Kevin Plaxco to develop electrochemical sensors based on oligonucleotide receptors (aptamers). In 2007, she joined Professor H.T. Soh's laboratory at UCSB as a Research Professor, developing new methods to generate aptamers via SELEX. Dr. Xiao joined Florida International University in 2011 as an Assistant Professor and was promoted to tenured Associate Professor in 2017. She recently moved to the NC State Department of Chemistry as a tenured Associate Professor.

Works (160)

Updated: April 4th, 2024 02:26

2024 article

Developing Aptamer-Based Colorimetric Opioid Tests

Canoura, J., Alkhamis, O., Venzke, M., Ly, P. T., & Xiao, Y. (2024, March 1). JACS AU, Vol. 3.

By: J. Canoura n, O. Alkhamis n, M. Venzke n, P. Ly n & Y. Xiao n

author keywords: aptamers; SELEX; opioids; high-throughputsequencing; exonuclease; dye-displacement assay; 3,3'-di(3-sulfopropyl)-4,5,4',5'-dibenzo-9-methyl-thiacarbocyanine; Marquis test
TL;DR: This work develops on-site sensors for morphine and structurally related opioid compounds based on in vitro-selected oligonucleotide affinity reagents known as aptamers that enable the naked-eye identification of specific opioids within seconds and will play an important role in combatting opioid abuse. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: March 18, 2024

2024 article

Exploring the Landscape of Aptamers: From Cross-Reactive to Selective to Specific, High-Affinity Receptors for Cocaine

Yang, K., Alkhamis, O., Canoura, J., Bryant, A., Gong, E. M., Barbu, M., … Landry, D. W. (2024, February 13). JACS AU, Vol. 2.

By: K. Yang*, O. Alkhamis n, J. Canoura n, A. Bryant n, E. Gong*, M. Barbu*, S. Taylor*, D. Nikic* ...

author keywords: cocaine; aptamer; specific; high-affinity; cross-reactive; hydrophobic receptor
TL;DR: A series of progressively improved DNA aptamers recognizing cocaine are reported, with the final optimized receptors having low nanomolar affinity and over a thousand-fold selectivity over the initial cross-reactants. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Web Of Science, ORCID
Added: March 11, 2024

2024 journal article

High-Affinity Aptamers for <i>In Vitro</i> and <i>In Vivo</i> Cocaine Sensing

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 146(5), 3230–3240.

TL;DR: High-stringency in vitro selection is performed to generate DNA aptamers that bind cocaine with nanomolar affinity and clinically relevant specificity, thus representing a dramatic improvement over the current-generation, micromolar-affinity, low-specificity cocaine aptamers. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: February 19, 2024

2023 journal article

Aptamer-Based Fentanyl Detection in Biological Fluids

ANALYTICAL CHEMISTRY, 95(49), 18258–18267.

By: J. Canoura n, Y. Liu n, O. Alkhamis n & Y. Xiao n

TL;DR: This work utilized library-immobilized SELEX to isolate new aptamers─nucleic acid-based bioreceptors that are well-suited for biosensing that can specifically bind fentanyl under physiological conditions and incorporated one of these into an electrochemical aptamer-based sensor that can rapidly detect fentanyl. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Web Of Science, ORCID
Added: January 29, 2024

2023 journal article

Comparison of Aptamer Signaling Mechanisms Reveals Disparities in Sensor Response and Strategies to Eliminate False Signals

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 145(22), 12407–12422.

By: O. Alkhamis n, J. Canoura n, C. Willis n, L. Wang n, J. Perry n & Y. Xiao n

MeSH headings : Aptamers, Nucleotide / chemistry; DNA; DNA, Complementary; Sensitivity and Specificity; SELEX Aptamer Technique / methods; Biosensing Techniques / methods
TL;DR: This work has developed a series of sensors using aptamers for the small-molecule drugs flunixin, fentanyl, and furanyl fentanyl and compares their performance, focusing on their specificity, and demonstrates strategies for improving the sensitivity and specificity of aptamer sensors with the development of a "hybrid beacon". (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: July 19, 2023

2023 article

Suite of Aptamer-Based Sensors for the Detection of Fentanyl and Its Analogues

Canoura, J., Liu, Y., Perry, J., Willis, C., & Xiao, Y. (2023, April 25). ACS SENSORS, Vol. 4.

By: J. Canoura n, Y. Liu n, J. Perry n, C. Willis n & Y. Xiao n

author keywords: aptamers; fentanyl; analogue; electrochemistry; colorimetry; fluorescence; bioanalytical; sensors
MeSH headings : Humans; United States; Fentanyl; Analgesics, Opioid; Illicit Drugs; Drug Overdose; Immunoassay
TL;DR: A series of new aptamer-based assays and sensors that can detect fentanyl as well as several of its analogues in a reliable, accurate, rapid, and economic manner are developed and foresee the potential for routine use by medical and law enforcement personnel aswell as the general public to aid in rapid and accurate fentanyl identification. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: May 30, 2023

2023 article

Using Exonucleases for Aptamer Characterization, Engineering, and Sensing

Alkhamis, O., Canoura, J., Ly, P. T., & Xiao, Y. (2023, June 14). ACCOUNTS OF CHEMICAL RESEARCH, Vol. 6.

By: O. Alkhamis n, J. Canoura n, P. Ly n & Y. Xiao n

TL;DR: The serendipitously discovered that the digestion of DNA aptamers by exonucleases is inhibited when an aptamer is bound to a ligand, which served as the foundation for the development of three novel aptamer-related methodologies in the laboratory. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: June 26, 2023

2022 journal article

DNA Aptamer-Cyanine Complexes as Generic Colorimetric Small-Molecule Sensors

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 61(3).

By: O. Alkhamis n, J. Canoura n, K. Bukhryakov*, A. Tarifa*, A. DeCaprio* & Y. Xiao n

author keywords: aptamers; colorimetric detection; cyanine dyes; dye-displacement; small-molecule targets
TL;DR: A rational approach is demonstrated to develop colorimetric cyanine dye-displacement assays that can be broadly applied to DNA aptamers regardless of their structure, sequence, affinity, or the physicochemical properties of their targets. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: April 18, 2022

2022 article

High-throughput quantitative binding analysis of DNA aptamers using exonucleases

Canoura, J., Alkhamis, O., Liu, Y., Willis, C., & Xiao, Y. (2022, December 30). NUCLEIC ACIDS RESEARCH, Vol. 12.

By: J. Canoura n, O. Alkhamis n, Y. Liu n, C. Willis n & Y. Xiao n

MeSH headings : Aptamers, Nucleotide / chemical synthesis; Aptamers, Nucleotide / chemistry; Exonucleases; Ligands; Nucleic Acids; SELEX Aptamer Technique / methods; Fentanyl / analysis; Robotics
TL;DR: This work has developed a high-throughput method for accurately quantifying aptamer binding affinity, specificity, and cross-reactivity via the kinetics of aptamer digestion by exonucleases and this approach dramatically accelerates the aptamer characterization process and streamlines sensor development. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: January 17, 2023

2022 article

Near-Infrared Dye-Aptamer Assay for Small Molecule Detection in Complex Specimens

Jin, X., Liu, Y., Alkhamis, O., Canoura, J., Bacon, A., Xu, R., … Xiao, Y. (2022, July 7). ANALYTICAL CHEMISTRY, Vol. 7.

By: X. Jin*, Y. Liu n, O. Alkhamis n, J. Canoura n, A. Bacon n, R. Xu*, F. Fu*, Y. Xiao n

MeSH headings : Aptamers, Nucleotide / chemistry; Biological Assay; Biosensing Techniques / methods
TL;DR: The utility of dye-aptamer NIR biosensors for high-throughput detection of analytes in clinical specimens is shown and the generality of this assay is demonstrated by detecting three different small-molecule analytes with their respective DNA aptamers at clinically relevant concentrations in serum and urine. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: July 26, 2022

2022 article

Systematic Study of in Vitro Selection Stringency Reveals How To Enrich High-Affinity Aptamers

Alkhamis, O., & Xiao, Y. (2022, December 27). JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, Vol. 12.

By: O. Alkhamis n & Y. Xiao n

MeSH headings : Aptamers, Nucleotide / chemistry; SELEX Aptamer Technique / methods; Ligands
TL;DR: By performing four separate trials of SELEX for the same small-molecule target, it is experimentally proved that the use of excessively high target concentrations promotes enrichment of low-affinity binders while also suppressing the enrichment of high-Affinity aptamers. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: January 17, 2023

2021 journal article

Advances and Challenges in Small‐Molecule DNA Aptamer Isolation, Characterization, and Sensor Development

Angewandte Chemie International Edition, 60(31), 16800–16923.

By: H. Yu*, O. Alkhamis*, J. Canoura*, Y. Liu* & Y. Xiao*

author keywords: aptamers; biosensors; DNA; SELEX
MeSH headings : Aptamers, Nucleotide / chemistry; Aptamers, Nucleotide / isolation & purification; Biosensing Techniques; Equipment Design; SELEX Aptamer Technique
TL;DR: Aptamers are short oligonucleotides isolated in vitro from randomized libraries that can bind to specific molecules with high affinity, and offer a number of advantages relative to antibodies as biorecognition elements in biosensors, but it remains difficult and labor-intensive to develop aptamer-based sensors for small-molecule detection. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 27, 2021

2021 journal article

Aptamer-Integrated Multianalyte-Detecting Paper Electrochemical Device

ACS Applied Materials & Interfaces, 13(15), 17330–17339.

By: Y. Liu*, O. Alkhamis*, X. Liu*, H. Yu*, J. Canoura* & Y. Xiao*

author keywords: aptamer; small molecules; sensing; multianalyte detection; paper electrochemical device; ambient vacuum filtration
MeSH headings : Aptamers, Nucleotide / chemistry; Dimethylpolysiloxanes / chemistry; Electrochemistry / instrumentation; Nylons / chemistry; Paper; Vacuum
TL;DR: This work has developed a simple, inexpensive procedure for the fabrication of aptamer-modified multiplex PEDs (mPEDs), which can robustly and specifically detect multiple small molecules in complex samples, and demonstrates that the addition of a PDMS chamber allows them to achieve detection in microliter volumes of biological samples. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 27, 2021

2021 report

Aptamers that bind to natural and synthetic cannabinoids

(Patent No. US10907163B1).

By: H. Yu & Y. Xiao

Source: NC State University Libraries
Added: July 27, 2021

2021 conference paper

Development of Aptamer-Based Sensors for Sensitive and Specific Detection of Fentanyl Opioids

Presented at the Pittcon Conference and Expo.

By: J. Canoura, Y. Liu & Y. Xiao

Event: Pittcon Conference and Expo on March 11, 2021

Source: NC State University Libraries
Added: July 27, 2021

2021 journal article

Immobilization Strategies for Enhancing Sensitivity of Electrochemical Aptamer-Based Sensors

ACS Applied Materials & Interfaces, 13(8), 9491–9499.

By: Y. Liu*, J. Canoura*, O. Alkhamis* & Y. Xiao*

author keywords: aptamer; electrochemistry; sensing; target-assisted aptamer immobilization; small molecules; bundling effect
MeSH headings : Aptamers, Nucleotide / chemistry; Biosensing Techniques / instrumentation; Biosensing Techniques / methods; Cocaine / analysis; Cocaine / chemistry; DNA / chemistry; Electrochemical Techniques / instrumentation; Electrochemical Techniques / methods; Electrodes; Gold / chemistry; Hydrogen-Ion Concentration; Immobilized Nucleic Acids / chemistry; Osmolar Concentration; Signal-To-Noise Ratio
TL;DR: This work demonstrated that immobilizing aptamers in their target-bound, folded state on gold electrode surfaces yields an aptamer monolayer that supports greater sensitivity and higher signal-to-noise ratio than traditionally prepared E-AB sensors. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

2021 journal article

Isolation of Natural DNA Aptamers for Challenging Small-Molecule Targets, Cannabinoids

Analytical Chemistry, 93(6), 3172–3180.

By: H. Yu*, Y. Luo*, O. Alkhamis*, J. Canoura*, B. Yu* & Y. Xiao*

MeSH headings : Aptamers, Nucleotide; Cannabinoids; Gene Library; Ligands; SELEX Aptamer Technique
TL;DR: It is demonstrated that natural nucleic acid libraries can yield high-quality aptamers for small-molecule targets, and a robust workflow is outlined for isolating other such aptamer in future selection efforts. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Crossref, ORCID
Added: July 11, 2021

2021 report

Materials and methods for colorimetric detection of small molecule targets

(Patent No. US10948504B2).

By: H. Yu, Y. Luo & Y. Xiao

Source: NC State University Libraries
Added: July 27, 2021

2021 report

Method for isolating cross-reactive aptamer and use thereof

(Patent No. US10907162B2).

By: W. Yang, H. Yu, Y. Liu & Y. Xiao

Source: NC State University Libraries
Added: July 27, 2021

2021 article

Platinum-Nanoparticle-Modified Single-Walled Carbon Nanotube-Laden Paper Electrodes for Electrocatalytic Oxidation of Methanol

Paudyal, J., Wang, P., Zhou, F., Liu, Y., Cai, Y., & Xiao, Y. (2021, December 15). ACS APPLIED NANO MATERIALS, Vol. 12.

By: J. Paudyal*, P. Wang*, F. Zhou*, Y. Liu*, Y. Cai* & Y. Xiao n

author keywords: paper electrode; vacuum filtration; single-walled carbon nanotube; platinum nanoclusters; electrochemical deposition; sensor
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: January 3, 2022

2020 journal article

Accelerating Post-SELEX Aptamer Engineering Using Exonuclease Digestion

Journal of the American Chemical Society, 143(2), 805–816.

By: J. Canoura*, H. Yu*, O. Alkhamis*, D. Roncancio*, R. Farhana* & Y. Xiao*

MeSH headings : Aptamers, Nucleotide / chemistry; Aptamers, Nucleotide / metabolism; Digestion; Escherichia coli / enzymology; Exodeoxyribonucleases / chemistry; Exodeoxyribonucleases / metabolism; SELEX Aptamer Technique
UN Sustainable Development Goal Categories
16. Peace, Justice and Strong Institutions (OpenAlex)
Sources: Crossref, ORCID
Added: July 11, 2021

2020 conference paper

Achieving Near-ideal Binding Spectra using Subideal Bioreceptors

Presented at the Pittcon 2020, Chicago, Illinois.

By: Y. Liu, H. Yu, O. Alkhamis, J. Moliver & Y. Xiao

Event: Pittcon 2020 at Chicago, Illinois on March 1-5, 2020

Source: NC State University Libraries
Added: July 27, 2021

2020 speech

Beauty Through Simplicity – Problem-Driven Aptamer Science

Invited talk presented at the NC State University, Raleigh, NC.

By: Y. Xiao

Event: at NC State University, Raleigh, NC on January 9-10, 2020

Source: NC State University Libraries
Added: July 27, 2021

2020 journal article

Fabrication of Aptamer‐Modified Paper Electrochemical Devices for On‐Site Biosensing

Angewandte Chemie International Edition, 60(6), 2993–3000.

By: H. Yu*, Z. Chen*, Y. Liu*, O. Alkhamis*, Z. Song* & Y. Xiao*

author keywords: aptamers; biosensors; nanomaterials; paper electrochemical devices; small molecules
MeSH headings : Aptamers, Nucleotide / chemistry; Aptamers, Nucleotide / metabolism; Biosensing Techniques / instrumentation; Biosensing Techniques / methods; Electrochemical Techniques / instrumentation; Electrodes; Gold / chemistry; Metal Nanoparticles / chemistry; Paper
TL;DR: A simple, economic, and environmentally-friendly strategy for fabricating aptamer-modified paper electrochemical devices (PEDs) via ambient vacuum filtration is demonstrated that can lead to the development of high performance PEDs that enable on-site detection of a variety of analytes, simplifying theDevelopment of portable sensors for real-world applications. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

2020 conference paper

Isolation of Class-specific Aptamers and Development of Sensors for Instantaneous Detection of Synthetic Cathinones

Presented at the Pittcon, Chicago, IL.

By: Y. Xiao

Event: Pittcon at Chicago, IL on March 1-5, 2020

Source: NC State University Libraries
Added: July 27, 2021

2020 journal article

Label-free profiling of DNA aptamer-small molecule binding using T5 exonuclease

Nucleic Acids Research, 48(20), e120–e120.

By: O. Alkhamis*, W. Yang*, R. Farhana*, H. Yu* & Y. Xiao*

MeSH headings : Aptamers, Nucleotide / chemistry; DNA / chemistry; Exodeoxyribonucleases / chemistry; Inverted Repeat Sequences; Ligands; Methamphetamine / analogs & derivatives; Methamphetamine / chemistry; Protein Binding; SELEX Aptamer Technique / methods
TL;DR: This work describes a novel method for accurately and sensitively evaluating the binding between DNA aptamers and small-molecule ligands in a high-throughput format without any aptamer engineering or labeling requirements, based on the new finding that ligand binding inhibits aptamer digestion by T5 exonuclease. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

2020 report

Materials and methods for colorimetric detection of small molecule targets

(Patent No. US10725058B2).

By: Y. Xiao, Y. Luo & H. Yu

Source: NC State University Libraries
Added: July 27, 2021

2020 report

Materials and methods for rapid and sensitive detection of small-molecule targets

(Patent No. US10550395B2).

By: Y. Xiao & H. Yu

Source: NC State University Libraries
Added: July 27, 2021

2020 report

Materials and methods for rapid and specific detection of synthetic cathinones

(Patent No. US10683507B2).

By: Y. Xiao & H. Yu

Source: NC State University Libraries
Added: July 27, 2021

2020 report

Method for isolating cross-reactive aptamer and use thereof

(Patent No. US10655132B1).

By: W. Yang, H. Yu, Y. Liu & Y. Xiao

Source: NC State University Libraries
Added: July 27, 2021

2020 conference paper

Screening of aptamer mutants for enhanced target specificity using a dual exonuclease digestion assay

Presented at the Pittcon 2020, Chicago, Illinois.

By: J. Canoura, R. Farhana, R. Roncancio & Y. Xiao

Event: Pittcon 2020 at Chicago, Illinois on March 1-5, 2020

Source: NC State University Libraries
Added: July 27, 2021

2020 journal article

Tuning Biosensor Cross-Reactivity Using Aptamer Mixtures

Analytical Chemistry, 92(7), 5041–5047.

By: Y. Liu*, H. Yu*, O. Alkhamis*, J. Moliver* & Y. Xiao*

MeSH headings : Alkaloids / analysis; Aptamers, Nucleotide / chemistry; Benzothiazoles / chemistry; Biosensing Techniques; Carbocyanines / chemistry; Electrochemical Techniques; SELEX Aptamer Technique
TL;DR: This work presents a novel approach for controlling the cross-reactivity of biosensors by employing defined mixtures of aptamers with differing binding properties, and believes it offers a generally applicable method for generating near-ideal aptamer biosensor for various analytical applications, including medical diagnostics, environmental monitoring, and drug detection. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

2019 conference paper

A Cooperative-Binding Split Aptamer Assay for Rapid, Specific and Ultra-Sensitive Fluorescence Detection of Cocaine in Saliva

Presented at the Pittcon 2019, Philadelphia, Pennsylvania.

By: H. Yu, J. Canoura, B. Guntupalli & Y. Xiao

Event: Pittcon 2019 at Philadelphia, Pennsylvania on March 17-21, 2019

Source: NC State University Libraries
Added: July 27, 2021

2019 conference paper

Beauty Through Simplicity – Functionalized Aptamers for On-Site Small-Molecule Detection

Presented at the Pittcon 2019, Philadelphia, Pennsylvania.

By: Y. Xiao

Event: Pittcon 2019 at Philadelphia, Pennsylvania on March 17-21, 2019

Source: NC State University Libraries
Added: July 27, 2021

2019 conference paper

Development of Electrochemical Aptamer-Based Sensors for On-Site Synthetic Cathinone Detection

Presented at the Pittcon 2019, Philadelphia, Pennsylvanis.

By: Y. Liu, O. Alkhamis, H. Yu & Y. Xiao

Event: Pittcon 2019 at Philadelphia, Pennsylvanis on March 17-21, 2019

Source: NC State University Libraries
Added: July 27, 2021

2019 report

Highly conductive porous paper-based metal thin films

(Patent No. US10290386B2).

By: Y. Xiao, B. Guntupalli & J. Paudyal

Source: NC State University Libraries
Added: July 27, 2021

2019 conference paper

In Vitro Isolation of Small-Molecule-Binding Aptamers with Intrinsic Dye-Displacement Functionality

Presented at the Pittcon 2019, Philadelphia, Pennsylvania.

By: H. Yu, W. Yang, O. Alkhamis, J. Canoura, K. Yang & Y. Xiao

Event: Pittcon 2019 at Philadelphia, Pennsylvania on March 17-21, 2019

Source: NC State University Libraries
Added: July 27, 2021

2019 journal article

In vitro isolation of class-specific oligonucleotide-based small-molecule receptors

Nucleic Acids Research, 47(12), e71–e71.

By: W. Yang*, H. Yu*, O. Alkhamis*, Y. Liu*, J. Canoura*, F. Fu*, Y. Xiao*

MeSH headings : Alkaloids / chemistry; Aptamers, Nucleotide / chemistry; Aptamers, Nucleotide / isolation & purification; Aptamers, Nucleotide / metabolism; High-Throughput Nucleotide Sequencing; SELEX Aptamer Technique / methods; Sequence Analysis, DNA
TL;DR: A novel ‘parallel-and-serial’ selection strategy for isolating class-specific oligonucleotide-based receptors (aptamers) in vitro that significantly expand the binding capabilities of aptamers as class- specific bioreceptors and further demonstrate the power of rationally designed selection strategies for isolates customized aptamer with desired binding profiles. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

2019 journal article

Innovative engineering and sensing strategies for aptamer-based small-molecule detection

TrAC Trends in Analytical Chemistry, 121, 115699.

By: O. Alkhamis*, J. Canoura*, H. Yu*, Y. Liu* & Y. Xiao*

TL;DR: This paper will review new approaches for the streamlined development of high-performance aptamer-based sensors for small-molecule detection, with emphasis placed on new aptamer engineering methods, the use of cooperative binding, and label-free approaches using fully-folded, high-affinity aptamers forSmall-Molecule sensing. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

2019 conference paper

Introducing Structure-Switching Functionality into Small-Molecule-Binding Aptamers via Nuclease-Directed Truncation

Presented at the Pittcon 2019, Philadelphia, Pennsylvania.

By: Z. Wang, H. Yu, J. Canoura, Y. Liu, O. Alkhamis, F. Fu, Y. Xiao

Event: Pittcon 2019 at Philadelphia, Pennsylvania on March 17-21, 2019

Source: NC State University Libraries
Added: July 27, 2021

2019 journal article

Label-Free, Visual Detection of Small Molecules Using Highly Target-Responsive Multimodule Split Aptamer Constructs

Analytical Chemistry, 91(11), 7199–7207.

By: Y. Luo*, H. Yu*, O. Alkhamis*, Y. Liu*, X. Lou*, B. Yu*, Y. Xiao*

MeSH headings : Aptamers, Nucleotide / chemistry; Aptamers, Nucleotide / metabolism; Benzodioxoles / analysis; Biosensing Techniques; Cocaine / analysis; Colorimetry; DNA, Catalytic / chemistry; DNA, Catalytic / metabolism; Protein Engineering; Pyrrolidines / analysis; Small Molecule Libraries / analysis; Synthetic Cathinone
TL;DR: This work reports a generalizable strategy for engineering novel multimodule split DNA constructs termed "CBSAzymes" that utilize a cooperative binding split aptamer (CBSA) as a highly target-responsive bioreceptor and a new, highly active split DNAzyme as an efficient signal reporter. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Crossref, ORCID
Added: July 11, 2021

2019 journal article

Perspective on the Future Role of Aptamers in Analytical Chemistry

Analytical Chemistry, 91(24), 15335–15344.

MeSH headings : Affinity Labels; Aptamers, Nucleotide / chemistry; Ligands; Nucleic Acids / chemistry; SELEX Aptamer Technique / methods; SELEX Aptamer Technique / trends
TL;DR: This perspective covers recent advances in SELEX methodology coupled with post-selection procedures all aimed at enhancing affinity and specificity of the selected aptamers - what this paper views as a critical barrier in the future role of aptamer in analytical chemistry. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

2019 conference paper

Rapid, naked-eye screening for synthetic cathinones based on cross-reactive aptamers

Presented at the Pittcon 2019, Philadelphia, Pennsylvania.

By: W. Yang, H. Yu, O. Alkhamis, F. Fu & Y. Xiao

Event: Pittcon 2019 at Philadelphia, Pennsylvania on March 17-21, 2019

Source: NC State University Libraries
Added: July 27, 2021

2019 journal article

Universal Design of Structure-Switching Aptamers with Signal Reporting Functionality

Analytical Chemistry, 91(22), 14514–14521.

By: H. Gao*, J. Zhao*, Y. Huang*, X. Cheng*, S. Wang*, Y. Han*, Y. Xiao*, X. Lou*

MeSH headings : Aptamers, Nucleotide / chemistry; Benzothiazoles / chemistry; Biocatalysis; Biosensing Techniques / methods; Cocaine / analysis; Cocaine / chemistry; Colorimetry / methods; DNA, Catalytic / chemistry; DNA, Catalytic / genetics; Estradiol / analysis; Estradiol / chemistry; Fluorescent Dyes / chemistry; G-Quadruplexes; Limit of Detection; Mercury / analysis; Mercury / chemistry; Nucleic Acid Conformation; Peroxidase / chemistry; Spectrometry, Fluorescence / methods; Sulfadimethoxine / analysis; Sulfadimethoxine / chemistry; Thrombin / analysis; Thrombin / chemistry
TL;DR: It is demonstrated that diverse aptamers can be readily engineered into G4-SSA constructs independent of target class, binding affinity, aptamer length, or structure, which could broadly extend the power, accessibility and utility of numerous SSA-based biosensors. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

2018 conference paper

Aptamer-based Assays for On-site Drug Detection

Presented at the Pittcon 2018, Orlando, Florida.

By: Y. Xiao

Event: Pittcon 2018 at Orlando, Florida on February 26 - March 1, 2018

Source: NC State University Libraries
Added: July 27, 2021

2018 speech

Beauty through simplicity – functionalized aptamers for on-site small-molecule detection

Invited talk presented at the University of Notre Dame, South Bend, Indiana.

By: Y. Xiao

Event: at University of Notre Dame, South Bend, Indiana on September 17, 2018

Source: NC State University Libraries
Added: July 27, 2021

2018 journal article

Dithiothreitol-Regulated Coverage of Oligonucleotide-Modified Gold Nanoparticles To Achieve Optimized Biosensor Performance

ACS Applied Materials & Interfaces, 10(4), 4233–4242.

By: P. Liang*, J. Canoura*, H. Yu*, O. Alkhamis* & Y. Xiao*

author keywords: gold nanoparticles; DNA surface coverage; dithiothreitol; enzyme-assisted target recycling; DNA detection; cocaine detection
MeSH headings : Biosensing Techniques; Colorimetry; Dithiothreitol; Gold; Metal Nanoparticles
TL;DR: This work develops a single-step assay utilizing dithiothreitol (DTT) to fine-tune the surface coverage of DNA-modified AuNPs, and demonstrates the effects of surface coverage on AuNP-based sensors using DTT-regulated DNA- modified AuNBP. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Crossref, ORCID
Added: July 11, 2021

2018 journal article

In vitro isolation of small-molecule-binding aptamers with intrinsic dye-displacement functionality

Nucleic Acids Research, 46(8), e43–e43.

By: H. Yu*, W. Yang*, O. Alkhamis*, J. Canoura*, K. Yang* & Y. Xiao*

MeSH headings : Aptamers, Nucleotide / chemistry; Aptamers, Nucleotide / genetics; Aptamers, Nucleotide / isolation & purification; Base Sequence; Benzodioxoles / analysis; Biosensing Techniques / methods; Carbocyanines / chemistry; Colorimetry / methods; Coloring Agents / chemistry; DNA / chemistry; DNA / genetics; Molecular Structure; Pyrrolidines / analysis; SELEX Aptamer Technique / methods; Synthetic Cathinone
TL;DR: A simple strategy for directly isolating signal-reporting aptamers in vitro through systematic evolution of ligands by exponential enrichment that transduce binding events into a detectable change of absorbance via target-induced displacement of a small-molecule dye. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Crossref, ORCID
Added: July 11, 2021

2018 journal article

Introducing structure-switching functionality into small-molecule-binding aptamers via nuclease-directed truncation

Nucleic Acids Research, 46(13), e81–e81.

By: Z. Wang*, H. Yu*, J. Canoura*, Y. Liu*, O. Alkhamis*, F. Fu*, Y. Xiao*

MeSH headings : Adenosine Triphosphate / analysis; Aptamers, Nucleotide / chemistry; Cocaine / analysis; Exodeoxyribonucleases; Mutation; Spectrometry, Fluorescence
TL;DR: The authors' Exo III-digestion method should be applicable for the generation of structure-switching aptamers from other TWJ- or stem–loop-containing small-molecule-binding aptamer, greatly simplifying thegeneration of functionalized sensor elements for folding-based aptasensors. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Crossref, ORCID
Added: July 11, 2021

2018 report

Materials and methods for rapid visualization of NAD(P)H

(Patent No. US10000790B2).

By: P. Liang, H. Yu & Y. Xiao

Source: NC State University Libraries
Added: July 27, 2021

2018 journal article

No Structure-Switching Required: A Generalizable Exonuclease-Mediated Aptamer-Based Assay for Small-Molecule Detection

Journal of the American Chemical Society, 140(31), 9961–9971.

By: J. Canoura*, Z. Wang*, H. Yu*, O. Alkhamis*, F. Fu* & Y. Xiao*

MeSH headings : Aptamers, Nucleotide / chemistry; Calorimetry / methods; Cocaine / chemistry; DNA / chemistry; Exodeoxyribonucleases / chemistry; Fluorescent Dyes / chemistry
TL;DR: It is shown that the digestion of aptamers by exonuclease III can likewise be inhibited upon binding of small-molecule targets and exploited for the first time to achieve sensitive, label-free small-Molecule detection. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Crossref, ORCID
Added: July 11, 2021

2018 conference paper

Rapid and sensitive detection of small-molecule targets via enzyme-assisted target recycling based on cooperative binding split aptamers

Presented at the Pittcon 2018, Orlando, Florida.

By: H. Yu, J. Canoura, B. Guntupalli, X. Lou & Y. Xiao

Event: Pittcon 2018 at Orlando, Florida on February 26 - March 1, 2018

Source: NC State University Libraries
Added: July 27, 2021

2018 journal article

Sensitive Detection of Small-Molecule Targets Using Cooperative Binding Split Aptamers and Enzyme-Assisted Target Recycling

Analytical Chemistry, 90(3), 1748–1758.

By: H. Yu*, J. Canoura*, B. Guntupalli*, O. Alkhamis* & Y. Xiao*

MeSH headings : Aptamers, Nucleotide / chemistry; Biosensing Techniques / methods; Colorimetry / methods; DNA / chemistry; Dehydroepiandrosterone Sulfate / analysis; Exodeoxyribonucleases / chemistry; Gold / chemistry; Limit of Detection; Metal Nanoparticles / chemistry; Nucleic Acid Amplification Techniques / methods
TL;DR: An instrument-free colorimetric assay employing EATR-mediated aggregation of CBSA-modified gold nanoparticles for the visual detection of low-micromolar concentrations of cocaine is developed and it is believed that such assays should prove valuable for detecting small-molecule targets in diverse fields. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

2018 conference paper

Utilizing nuclease screening of ligand-aptamer complexes to enhance specificity of an aptamer-based cocaine assay

Presented at the 2017 Society of Forensic Toxicologists-International Association of Forensic Toxicologists (SOFT-TIAFT) Joint Meeting, Boca Raton, Florida.

By: Z. Wang, J. Canoura, H. Yu & Y. Xiao

Event: 2017 Society of Forensic Toxicologists-International Association of Forensic Toxicologists (SOFT-TIAFT) Joint Meeting at Boca Raton, Florida on January 7-11, 2018

Source: NC State University Libraries
Added: July 27, 2021

2017 journal article

A Broadly Applicable Assay for Rapidly and Accurately Quantifying DNA Surface Coverage on Diverse Particles

Bioconjugate Chemistry, 28(4), 933–943.

By: H. Yu*, X. Xu*, P. Liang*, K. Loh*, B. Guntupalli*, D. Roncancio*, Y. Xiao*

MeSH headings : Base Sequence; DNA Probes / analysis; DNA Probes / metabolism; Exodeoxyribonucleases / metabolism; Fluorescent Dyes / chemistry; Fluorescent Dyes / metabolism; Gold / chemistry; Immobilized Nucleic Acids / analysis; Immobilized Nucleic Acids / metabolism; Metal Nanoparticles / chemistry; Nucleic Acid Hybridization / methods; Static Electricity; Surface Properties
TL;DR: This work has developed a simple and broadly applicable exonuclease III (Exo III) digestion assay based on the cleavage of phosphodiester bonds to accurately quantify DNA probe surface coverage on diverse, commonly used particles of different compositions, conjugation chemistries, and sizes. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

2017 journal article

A cooperative-binding split aptamer assay for rapid, specific and ultra-sensitive fluorescence detection of cocaine in saliva

Chemical Science, 8(1), 131–141.

By: H. Yu*, J. Canoura*, B. Guntupalli*, X. Lou* & Y. Xiao*

Sources: Crossref, ORCID
Added: July 11, 2021

2017 conference paper

A cooperative-binding split aptamer assay for rapid, specific and ultra-sensitive fluorescence detection of cocaine in saliva

Poster presented at the Gordon Research Conference: Nucleosides, Nucleotides & Oligonucleotides, Salve Regina University, Newport, RI.

By: H. Yu, J. Canoura, B. Guntupalli & Y. Xiao

Event: Gordon Research Conference: Nucleosides, Nucleotides & Oligonucleotides at Salve Regina University, Newport, RI on June 25-30, 2017

Source: NC State University Libraries
Added: September 6, 2021

2017 conference paper

A label-free aptamer-fluorophore assembly for highly sensitive and specific detection of cocaine

Presented at the Pittcon 2017, Chicago, Illinois.

By: D. Roncancio, H. Yu, X. Xu & Y. Xiao

Event: Pittcon 2017 at Chicago, Illinois on March 5-9, 2017

Source: NC State University Libraries
Added: September 11, 2021

2017 conference paper

Cooperative-binding split aptamer assays for rapid, specific and ultra-sensitive detection of small-molecule targets in biofluid samples

Invited talk presented at the 2017 American Chemical Society Florida Annual Meeting and Exposition, Tampa, FL.

By: H. Yu, J. Canoura, B. Guntupalli & Y. Xiao

Event: 2017 American Chemical Society Florida Annual Meeting and Exposition at Tampa, FL on May 5-6, 2017

Source: NC State University Libraries
Added: September 6, 2021

2017 conference paper

Gold nanoparticle-based assays for rapid and sensitive colorimetric and electrochemical biosensing

Presented at the Nano Florida 2017, Florida International University, Miami, FL.

By: Y. Xiao

Event: Nano Florida 2017 at Florida International University, Miami, FL on September 23-24, 2017

Source: NC State University Libraries
Added: August 27, 2021

2017 speech

Gold nanoparticle-based, paper devices for rapid and sensitive colorimetric and electrochemical biosensing

Invited talk presented at the 2017 American Chemical Society Florida Annual Meeting and Exposition, Tampa, FL.

By: P. Liang, B. Guntupalli, H. Yu & Y. Xiao

Event: 2017 American Chemical Society Florida Annual Meeting and Exposition at Tampa, FL on May 5-6, 2017

Source: NC State University Libraries
Added: September 6, 2021

2017 report

Materials and methods for rapid and specific detection of cocaine

(Patent No. US9804178B2).

By: D. Roncancio, H. Yu, X. Xu & Y. Xiao

Source: NC State University Libraries
Added: July 27, 2021

2017 conference paper

Rapid and sensitive detection of small-molecule targets via enzyme-assisted target recycling based on cooperative binding split aptamers

Poster presented at the Gordon Research Conference: Nucleosides, Nucleotides & Oligonucleotides, Salve Regina University, Newport, RI.

By: H. Yu, J. Canoura, B. Guntupalli, X. Lou & Y. Xiao

Event: Gordon Research Conference: Nucleosides, Nucleotides & Oligonucleotides at Salve Regina University, Newport, RI on June 25-30, 2017

Source: NC State University Libraries
Added: September 6, 2021

2017 conference paper

Utilizing dual-enzyme selectivity for the identification of aptamer binding domains and the development of sensitive fluorescence detection of small-molecule targets

Presented at the FIU Annual Scholarly Forum, Graduate Student Appreciation Week (GSAW), Florida International University, Miami, FL.

By: J. Canoura, Z. Wang, H. Yu & Y. Xiao

Event: FIU Annual Scholarly Forum, Graduate Student Appreciation Week (GSAW) at Florida International University, Miami, FL on March 27-28, 2017

Source: NC State University Libraries
Added: September 11, 2021

2017 conference paper

Utilizing nuclease screening of ligand-aptamer complexes to enhance specificity of an aptamer-based cocaine assay

Poster presented at the Gordon Research Conference: Nucleosides, Nucleotides & Oligonucleotides, Salve Regina University, Newport, RI.

By: J. Canoura, Z. Wang, H. Yu & Y. Xiao

Event: Gordon Research Conference: Nucleosides, Nucleotides & Oligonucleotides at Salve Regina University, Newport, RI on June 25-30, 2017

Source: NC State University Libraries
Added: September 6, 2021

2016 conference paper

A cooperative-binding split aptamer assay for rapid, specific and ultra-sensitive fluorescence detection of cocaine in saliva

Presented at the Pittcon 2017, Chicago, Illinois.

By: H. Yu, J. Canoura, B. Guntupalli & Y. Xiao

Event: Pittcon 2017 at Chicago, Illinois on March 5-9, 2017

Source: NC State University Libraries
Added: September 11, 2021

2016 conference paper

A cooperative-binding split aptamer assay for rapid, specific and ultra-sensitive fluorescence detection of cocaine in saliva

Presented at the 2016 Conference of Undergraduate Research at FIU (CURFIU), Miami, FL.

By: H. Yu, B. Guntupalli & Y. Xiao

Event: 2016 Conference of Undergraduate Research at FIU (CURFIU) at Miami, FL on March 30-31, 2016

Source: NC State University Libraries
Added: September 11, 2021

2016 conference paper

A cooperative-binding split aptamer assay for rapid, specific and ultra-sensitive fluorescence detection of cocaine in saliva

Presented at the FIU Annual Scholarly Forum, Graduate Student Appreciation Week (GSAW), Florida International University, Miami, FL.

By: Y. H.X., G. B. & X. Y

Event: FIU Annual Scholarly Forum, Graduate Student Appreciation Week (GSAW) at Florida International University, Miami, FL on March 28-29, 2016

Source: NC State University Libraries
Added: September 11, 2021

2016 conference paper

A label-free aptamer-fluorophore assembly for highly sensitive and specific detection of cocaine

Presented at the 2016 Conference of Undergraduate Research at FIU (CURFIU), Miami, FL.

By: D. Roncancio, H. Yu, X. Xu, S. Wu, R. Liu, J. Debord, X. Lou, Y. Xiao

Event: 2016 Conference of Undergraduate Research at FIU (CURFIU) at Miami, FL on March 30-31, 2016

Source: NC State University Libraries
Added: September 11, 2021

2016 conference paper

Ambient filtration method to rapidly prepare highly conductive, paper-based porous gold films for electrochemical biosensing

Presented at the 2016 Conference of Undergraduate Research at FIU (CURFIU), Miami, FL.

By: B. Guntupalli, P. Liang, J. Lee, Y. Yang, H. Yu, J. Canoura, J. He, W. Li, Y. Weizmann, Y. Xiao

Event: 2016 Conference of Undergraduate Research at FIU (CURFIU) at Miami, FL on March 30-31, 2016

Source: NC State University Libraries
Added: September 11, 2021

2016 speech

Bringing biosensors to the bedside

Invited talk presented at the University of Miami, Medical Campus - Batchelor Children's Research Institute, Miami, FL.

By: Y. Xiao

Event: at University of Miami, Medical Campus - Batchelor Children's Research Institute, Miami, FL on May 18, 2016

Source: NC State University Libraries
Added: September 11, 2021

2016 conference paper

Paper-based device for rapid visualization of NADH based on dissolution of gold nanoparticles

Poster presented at the 2016 Conference of Undergraduate Research at FIU (CURFIU), Miami, FL.

By: P. Liang, H. Yu, B. Guntupalli & Y. Xiao

Event: 2016 Conference of Undergraduate Research at FIU (CURFIU) at Miami, FL on March 30-31, 2016

Source: NC State University Libraries
Added: September 11, 2021

2016 conference paper

Paper-based devices for rapid and sensitive colorimetric and electrochemical biosensing

Invited talk presented at the 2016 ACS Florida Annual Meeting and Exposition, Tampa, FL.

By: Y. Xiao

Event: 2016 ACS Florida Annual Meeting and Exposition at Tampa, FL on May 5-7, 2016

Source: NC State University Libraries
Added: September 11, 2021

2016 journal article

Rapid, Surfactant-Free, and Quantitative Functionalization of Gold Nanoparticles with Thiolated DNA under Physiological pH and Its Application in Molecular Beacon-Based Biosensor

ACS Applied Materials & Interfaces, 8(40), 27298–27304.

author keywords: gold nanoparticle; thiolated DNA; DNA-gold nanoparticle conjugates; ethylene glycol spacer; physiological pH; adsorption kinetics; quantitative adsorption
MeSH headings : Biosensing Techniques; DNA; Gold; Hydrogen-Ion Concentration; Metal Nanoparticles; Surface-Active Agents
TL;DR: An oligoethylene glycol (OEG) spacer-assisted method that enables quantitative and instantaneous attachment at physiological pH without the need for surfactants, and allows for the direct immobilization of thiolated molecular beacons, and avoids particle aggregation due to intermolecular hydrogen bonding. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Crossref, ORCID
Added: July 11, 2021

2016 conference paper

Utilizing nuclease screening of ligand-aptamer complexes to enhance specificity of an aptamer-based cocaine assay

Poster presented at the 2016 ACS Florida Annual Meeting and Exposition, Tampa, FL.

By: J. Canoura, Z. Wang, H. Yu, B. Ng, D. Roncancio & Y. Xiao

Event: 2016 ACS Florida Annual Meeting and Exposition at Tampa, FL on May 5-7, 2016

Source: NC State University Libraries
Added: September 11, 2021

2015 conference paper

A paper-based device for rapid visualization of NADH based on dissolution of gold nanoparticles

Poster presented at the 2015 Materials Research Society (MRS) Fall Meeting & Exhibit, Boston, Massachusetts.

By: P. Liang, H. Yu & Y. Xiao

Event: 2015 Materials Research Society (MRS) Fall Meeting & Exhibit at Boston, Massachusetts on November 29 - December 4, 2015

Source: NC State University Libraries
Added: September 11, 2021

2015 journal article

Ambient Filtration Method To Rapidly Prepare Highly Conductive, Paper-Based Porous Gold Films for Electrochemical Biosensing

ACS Applied Materials & Interfaces, 7(49), 27049–27058.

By: B. Guntupalli*, P. Liang*, J. Lee*, Y. Yang*, H. Yu*, J. Canoura*, J. He*, W. Li*, Y. Weizmann*, Y. Xiao*

author keywords: gold nanoparticles; single-walled carbon nanotubes; paper-based hybrid porous gold film; vacuum filtration; electrocatalytic response; film conductivity
MeSH headings : Biosensing Techniques / methods; Filtration / methods; Gold / chemistry; Membranes, Artificial; Metal Nanoparticles / chemistry; Porosity
TL;DR: An ambient filtration method is described that provides a simple and novel way to generate rapidly porous and thin gold films without the need for sophisticated instruments, clean-room environments, and any postgrowth process or sintering steps. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

2015 conference paper

Ambient filtration method to rapidly prepare highly conductive, paper-based porous gold films

Presented at the 2015 Materials Research Society (MRS) Fall Meeting & Exhibit, Boston, Massachusetts.

By: B. Guntupalli, P. Liang, Y. Yang, J. He, W. Li & Y. Xiao

Event: 2015 Materials Research Society (MRS) Fall Meeting & Exhibit at Boston, Massachusetts on November 29 - December 4, 2015

Source: NC State University Libraries
Added: September 11, 2021

2015 conference paper

Amplified colorimetric detection of cocaine in oral fluid based on exonuclease-assisted aptamer strand recycling

Poster presented at the Society of Forensic Toxicologists (SOFT) 2015 Annual Meeting, Atlanta, Georgia.

By: Z. Wang, H. Yu, B. Ng, D. Roncancio, J. Canoura & Y. Xiao

Event: Society of Forensic Toxicologists (SOFT) 2015 Annual Meeting at Atlanta, Georgia on October 19-23, 2015

Source: NC State University Libraries
Added: September 11, 2021

2015 speech

Aptamer-based, exonuclease-amplified, paper device for point of collection screening of cocaine and methamphetamine in oral fluid

Invited talk presented at the Forensic Science R&D Grantees Meeting, Orlando, FL.

By: Y. Xiao

Event: Forensic Science R&D Grantees Meeting at Orlando, FL on February 17, 2015

Source: NC State University Libraries
Added: September 11, 2021

2015 conference paper

Base-pair mismatch detection

Invited talk presented at the FIU Biomolecular Sciences Institute Symposium on DNA Repair and Genome Stability: In honor of the 2015 Nobel Prize in Chemistry for DNA Repair, Miami, FL.

By: Y. Xiao

Event: FIU Biomolecular Sciences Institute Symposium on DNA Repair and Genome Stability: In honor of the 2015 Nobel Prize in Chemistry for DNA Repair at Miami, FL on December 16, 2015

Source: NC State University Libraries
Added: September 11, 2021

2015 speech

Bringing biosensors to the bedside

Recruitment talk presented at the Division of Health and Natural Sciences, Florida Memorial University, Miami, FL.

By: Y. Xiao

Event: at Division of Health and Natural Sciences, Florida Memorial University, Miami, FL on February 24, 2015

Source: NC State University Libraries
Added: September 11, 2021

2015 journal article

Nanoprobe-Enhanced, Split Aptamer-Based Electrochemical Sandwich Assay for Ultrasensitive Detection of Small Molecules

Analytical Chemistry, 87(15), 7712–7719.

By: T. Zhao*, R. Liu*, X. Ding*, J. Zhao*, H. Yu*, L. Wang*, Q. Xu*, X. Wang* ...

MeSH headings : Aptamers, Nucleotide / chemistry; Biosensing Techniques / methods; Cocaine / analysis; Electrochemical Techniques; Limit of Detection; Small Molecule Libraries / analysis
TL;DR: The near-elimination of background in NE-SAESA effectively improves assay sensitivity by ∼1,000-100-000-fold for ATP and cocaine detection, relative to equivalent SAESA, and the significantly improved binding affinity enables the detection of small molecule targets with unprecedented sensitivity. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Crossref, ORCID
Added: July 11, 2021

2015 journal article

Paper-Based Device for Rapid Visualization of NADH Based on Dissolution of Gold Nanoparticles

ACS Applied Materials & Interfaces, 7(27), 15023–15030.

By: P. Liang*, H. Yu*, B. Guntupalli* & Y. Xiao*

author keywords: dissolution of gold nanoparticles; dihydronicotinamide adenine dinucleotide; paper-based device; colorimetric screening; dehydrogenase inhibitors; NAD(+)-driven enzymatic reactions
MeSH headings : Colorimetry / instrumentation; Colorimetry / methods; Equipment Design; Equipment Failure Analysis; Gold / chemistry; Metal Nanoparticles / chemistry; Metal Nanoparticles / ultrastructure; NAD / analysis; NAD / chemistry; Paper; Reagent Strips; Reproducibility of Results; Sensitivity and Specificity
TL;DR: A paper-based device that enables rapid and sensitive room-temperature detection of dihydronicotinamide adenine dinucleotide (NADH) via a colorimetric readout and its value for monitoring NAD+-driven enzymatic reactions is described and demonstrated. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

2015 conference paper

Simple, rapid and ultra-sensitive fluorescence detection of cocaine in oral fluid based on cooperative binding split aptamer

Presented at the Society of Forensic Toxicologists (SOFT) 2015 Annual Meeting, Atlanta, Georgia.

By: H. Yu, Z. Wang, B. Ng, J. Canoura & Y. Xiao

Event: Society of Forensic Toxicologists (SOFT) 2015 Annual Meeting at Atlanta, Georgia on October 19-23, 2015

Source: NC State University Libraries
Added: September 11, 2021

2014 journal article

A Label-Free Aptamer-Fluorophore Assembly for Rapid and Specific Detection of Cocaine in Biofluids

Analytical Chemistry, 86(22), 11100–11106.

By: D. Roncancio*, H. Yu*, X. Xu*, S. Wu*, R. Liu*, J. Debord*, X. Lou*, Y. Xiao*

MeSH headings : Aptamers, Nucleotide / chemistry; Body Fluids / chemistry; Cocaine / analysis; Fluorescence; Fluorescent Dyes / chemistry; Humans; Naphthyridines / chemistry; Time Factors
TL;DR: A rapid and specific aptamer-based method for one-step cocaine detection with minimal reagent requirements is reported and it is demonstrated that this sensor can detect cocaine within seconds at concentrations as low as 200 nM, which is 50-fold lower than existing assays based on target-induced conformational change. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Crossref, ORCID
Added: July 11, 2021

2014 conference paper

A label-free aptamer-fluorophore assembly for highly sensitive and specific detection of cocaine

Poster presented at the 2014 Materials Research Society (MRS) Fall Meeting & Exhibit, Boston, Massachusetts.

By: D. Roncancio, S. Wu, H. Yu, X. Xu, R. Liu, J. Debord, X. Lou, Y. Xiao

Event: 2014 Materials Research Society (MRS) Fall Meeting & Exhibit at Boston, Massachusetts on November 30 - December 5, 2014

Source: NC State University Libraries
Added: September 11, 2021

2014 journal article

Amplified Single Base-Pair Mismatch Detection via Aggregation of Exonuclease-Sheared Gold Nanoparticles

Analytical Chemistry, 86(7), 3461–3467.

By: S. Wu*, P. Liang*, H. Yu*, X. Xu*, Y. Liu*, X. Lou*, Y. Xiao*

MeSH headings : Base Pair Mismatch; Exonucleases / chemistry; Gold / chemistry; Metal Nanoparticles; Polymorphism, Single Nucleotide; Spectrophotometry, Ultraviolet
TL;DR: A simple platform that enables sensitive, naked-eye detection of SNPs with minimal reagent and equipment requirements at room temperature within 15 min is presented and it is demonstrated that this system can effectively discriminate a range of different mismatches. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

2014 conference paper

Amplified single base-pair mismatch detection via the aggregation of exonuclease-sheared gold nanoparticles

Poster presented at the 2014 Materials Research Society (MRS) Fall Meeting & Exhibit, Boston, Massachusetts.

By: S. Wu, P. Liang, H. Yu, X. Xu, Y. Liu, X. Lou, Y. Xiao

Event: 2014 Materials Research Society (MRS) Fall Meeting & Exhibit at Boston, Massachusetts on November 30 - December 5, 2014

Source: NC State University Libraries
Added: September 11, 2021

2014 speech

Bringing biosensors to the bedside

Recruitment talk presented at the Department of Chemistry, St. Thomas University, Miami, FL.

By: Y. Xiao

Event: at Department of Chemistry, St. Thomas University, Miami, FL on November 5, 2014

Source: NC State University Libraries
Added: September 11, 2021

2014 conference paper

Target recycling-based assay for rapid and accurate determination of DNA surface coverage on gold nanoparticles

Presented at the 2014 Materials Research Society (MRS) Fall Meeting & Exhibit, Boston, Massachusetts.

By: X. Xu, B. Guntupalli, H. Yu & Y. Xiao

Event: 2014 Materials Research Society (MRS) Fall Meeting & Exhibit at Boston, Massachusetts on November 30 - December 5, 2014

Source: NC State University Libraries
Added: September 11, 2021

2013 conference paper

Ambient filtration method to prepare paper-based electroactive nanoporous gold thin film for detection of dopamine and serotonin

Presented at the 89th Florida Annual Meeting and Exposition, Palm Harbor, FL.

By: B. Guntupalli, P. Liang, J. Dai, Y. Yang, J. He, W. Li, Y. Xiao

Event: 89th Florida Annual Meeting and Exposition at Palm Harbor, FL on May 9-11, 2013

Source: NC State University Libraries
Added: September 11, 2021

2013 speech

Bringing biosensors to the bedside

Recruitment talk presented at the Department of Chemistry, University of Tampa, Tampa, FL.

By: Y. Xiao

Event: at Department of Chemistry, University of Tampa, Tampa, FL on November 22, 2013

Source: NC State University Libraries
Added: September 11, 2021

2013 journal article

Controlling the function of DNA nanostructures with specific trigger sequences

Chem. Commun., 49(4), 397–399.

By: S. Deborggraeve*, J. Dai*, Y. Xiao* & H. Soh*

MeSH headings : Base Pair Mismatch; Base Sequence; DNA / chemistry; DNA / genetics; DNA, Catalytic / chemistry; DNA, Catalytic / genetics; Models, Molecular; Molecular Sequence Data; Nanostructures / chemistry; Nucleic Acid Hybridization; Optical Imaging
TL;DR: A switchable DNAzyme (SDZ) that only becomes activated in the presence of a perfectly matched trigger sequence and operates effectively at room temperature is developed. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

2013 journal article

In Vitro Selection of Shape-Changing DNA Nanostructures Capable of Binding-Induced Cargo Release

ACS Nano, 7(11), 9675–9683.

By: S. Oh*, K. Plakos*, Y. Xiao*, M. Eisenstein* & H. Soh*

author keywords: allostery; microfluidics; in vitro selection; functional aptamer; ATP; ATMND; structure-switching
MeSH headings : Adenosine Triphosphate / chemistry; Allosteric Site; Base Sequence; Binding Sites; Cloning, Molecular; DNA / chemistry; Microfluidics; Molecular Conformation; Molecular Sequence Data; Nanostructures / chemistry; Nanotechnology / methods; Nucleic Acid Conformation; Nucleotides; Spectrometry, Fluorescence
TL;DR: It is believed that this selection approach could be generalized to generate synthetic nanostructures capable of selective and controlled release of other small-molecule cargos in response to a variety of triggers, for both research and clinical applications. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

2013 conference paper

Paper-based single-walled carbon nanotube thin film for catalytic detection of nicotinamide adenine dinucleotide in human blood

Invited talk presented at the 89th Florida Annual Meeting and Exposition, Palm Harbor, FL.

By: Y. Xiao, J. Dai, J. Paudyal, B. Guntupalli, P. Liang, F. Pubillones, Y. Yang, W. Li, X. Wang

Event: 89th Florida Annual Meeting and Exposition at Palm Harbor, FL on May 9-11, 2013

Source: NC State University Libraries
Added: September 11, 2021

2013 journal article

Self-Assembled DNA Monolayer Buffered Dynamic Ranges of Mercuric Electrochemical Sensor

Analytical Chemistry, 85(15), 7574–7580.

MeSH headings : Biosensing Techniques / methods; Buffers; DNA Probes / chemistry; Electric Conductivity; Electrochemistry; Feasibility Studies; Limit of Detection; Mercury / analysis; Mercury / chemistry; Nucleic Acid Hybridization; Surface Properties
TL;DR: A novel buffer self-assembled monolayer (BSAM) strategy, to fabricate sensors with extraordinarily broad DRs using a single recognition ligand, which exhibited great selectivity against the common interferential metal ions. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

2012 conference paper

Biosensors, biotechnologies and biomaterials

Presented at the 9th International Symposium on Persistent Toxic Substances, Miami, FL.

By: Y. Xiao

Event: 9th International Symposium on Persistent Toxic Substances at Miami, FL on October 23-27, 2012

Source: NC State University Libraries
Added: September 11, 2021

2012 journal article

Electrochemical DNA three-way junction based sensor for distinguishing chiral metallo-supramolecular complexes

Chemical Communications, 48(55), 6900.

MeSH headings : Coordination Complexes / chemistry; DNA / chemistry; Electrochemical Techniques; Electrodes; Ferric Compounds / chemistry; Stereoisomerism
TL;DR: A signal-on three-way junction based-electrochemical DNA sensor for distinguishing chiral metallo-supramolecular complexes with an enantioselective recognition ratio of about 3.5 is reported. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

2012 journal article

Selection is more intelligent than design: improving the affinity of a bivalent ligand through directed evolution

Nucleic Acids Research, 40(22), 11777–11783.

By: K. Ahmad*, Y. Xiao* & H. Soh*

MeSH headings : Aptamers, Nucleotide / chemistry; DNA, Single-Stranded / chemistry; Directed Molecular Evolution / methods; Ligands; Microfluidic Analytical Techniques; Nucleic Acid Conformation; Thrombin / antagonists & inhibitors
TL;DR: A directed evolution-based method for generating optimal bivalent aptamer that binds thrombin with an apparent dissociation constant (Kd) <10 pM, representing a ∼200-fold improvement in binding affinity over the monomeric aptamers and a ∼15-fold improved over the best designed bivalent construct is reported. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

2011 speech

Bringing biosensors to the bedside

Recruitment talk presented at the Department of Chemistry, Barry University, Miami, FL.

By: Y. Xiao

Event: at Department of Chemistry, Barry University, Miami, FL on November 21, 2011

Source: NC State University Libraries
Added: September 11, 2021

2011 journal article

Genetic Analysis of H1N1 Influenza Virus from Throat Swab Samples in a Microfluidic System for Point-of-Care Diagnostics

Journal of the American Chemical Society, 133(23), 9129–9135.

By: B. Ferguson, S. Buchsbaum, T. Wu*, K. Hsieh, Y. Xiao*, R. Sun*, H. Soh*

MeSH headings : DNA, Single-Stranded / genetics; DNA, Single-Stranded / isolation & purification; Electrochemistry / instrumentation; Influenza A Virus, H1N1 Subtype / genetics; Influenza A Virus, H1N1 Subtype / isolation & purification; Limit of Detection; Magnetics; Microfluidic Analytical Techniques; Pharynx / virology; Point-of-Care Systems; Protein Denaturation; RNA, Viral / genetics; RNA, Viral / isolation & purification; Reverse Transcriptase Polymerase Chain Reaction; Ribonucleoproteins / chemistry; Specimen Handling
TL;DR: The Magnetic Integrated Microfluidic Electrochemical Detector (MIMED) is presented that integrates sample preparation and electrochemical sensors in a monolithic disposable device to detect RNA-based virus directly from throat swab samples and demonstrates the detection of influenza H1N1 in throat swabs at loads as low as 10 TCID(50), 4 orders of magnitude below the clinical titer for this virus. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

2011 journal article

Improving Aptamer Selection Efficiency through Volume Dilution, Magnetic Concentration, and Continuous Washing in Microfluidic Channels

Analytical Chemistry, 83(17), 6883–6889.

By: S. Oh*, K. Ahmad*, M. Cho*, S. Kim*, Y. Xiao* & H. Soh*

MeSH headings : Aptamers, Nucleotide / chemistry; Magnetics; Microfluidic Analytical Techniques / methods; SELEX Aptamer Technique; Streptavidin / analysis
TL;DR: This method retrieves a small number of magnetic beads suspended in a large volume and concentrates them into a microfluidic chamber with minimal loss for continuous washing and obtained new DNA aptamer sequences with low nanomolar affinity that specifically bind to the SA proteins. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

2011 journal article

Measurement of Aptamer–Protein Interactions with Back-Scattering Interferometry

Analytical Chemistry, 83(23), 8867–8870.

By: I. Olmsted*, Y. Xiao*, M. Cho*, A. Csordas*, J. Sheehan*, J. Meiler*, H. Soh*, D. Bornhop*

MeSH headings : Aptamers, Nucleotide / chemistry; Aptamers, Nucleotide / metabolism; Humans; Interferometry; Light; Protein Binding; Solutions / chemistry; Thrombin / chemistry; Thrombin / metabolism
TL;DR: The quantitative measurement of aptamer-protein interactions using backscattering interferometry (BSI) is reported and it is shown that BSI can determine when distinct binding regions are accessed and preliminary modeling efforts suggest evidence for allosteric linkage between the two exosites. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

2011 journal article

Polarity-Switching Electrochemical Sensor for Specific Detection of Single-Nucleotide Mismatches

Angewandte Chemie International Edition, 50(47), 11176–11180.

author keywords: DNA; electrochemistry; sensors; single-nucleotide mismatch
MeSH headings : Base Pair Mismatch; Biosensing Techniques / instrumentation; Biosensing Techniques / methods; DNA / analysis; DNA / genetics; DNA Probes; Electrochemistry / instrumentation; Electrochemistry / methods; Electrodes; Polymorphism, Single Nucleotide
TL;DR: A single-step, room-temperature electrochemical sensor that detects single-nucleotide mismatches with a “polarity-switching” response and describes the design principles of the bipolar sensor and demonstrates its performance in discriminating SM and PM targets under various conditions. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

2011 journal article

Probing the Limits of Aptamer Affinity with a Microfluidic SELEX Platform

PLoS ONE, 6(11), e27051.

By: K. Ahmad*, S. Oh*, S. Kim*, F. McClellen*, Y. Xiao* & H. Soh*

Ed(s): M. Antopolsky

MeSH headings : Animals; Aptamers, Nucleotide / genetics; Aptamers, Nucleotide / metabolism; Base Sequence; Becaplermin; Microfluidic Analytical Techniques / methods; Protein Binding; Proto-Oncogene Proteins c-sis / metabolism; SELEX Aptamer Technique / instrumentation
TL;DR: This work has developed a microfluidic selection process (M-SELEX) that can be used to obtain high affinity aptamers against diverse protein targets and discovers novel aptamer sequences that bind to platelet derived growth factor B and thrombin and a new aptamer that binds to apolipoprotein E3. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

2011 journal article

Selection of phage-displayed peptides on live adherent cells in microfluidic channels

Proceedings of the National Academy of Sciences, 108(17), 6909–6914.

By: J. Wang*, Y. Liu*, T. Teesalu*, K. Sugahara*, V. Kotamrajua*, J. Adams*, B. Ferguson*, Q. Gong* ...

MeSH headings : Bacteriophage T7 / genetics; Cell Line, Tumor; Humans; Microfluidic Analytical Techniques / methods; Neuropilin-1 / antagonists & inhibitors; Neuropilin-1 / genetics; Neuropilin-1 / metabolism; Peptide Library
TL;DR: It is shown that, compared to conventional biopanning methods, microfluidic selection enables more efficient discovery of peptides with higher affinity and specificity by providing controllable and reproducible means for applying stringent selection conditions against minimal amounts of target cells without loss. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

2011 journal article

Two-Step, PCR-Free Telomerase Detection by Using Exonuclease III-Aided Target Recycling

ChemBioChem, 12(18), 2745–2747.

By: X. Zuo*, F. Xia*, A. Patterson*, H. Soh*, Y. Xiao* & K. Plaxco*

author keywords: cancer; nucleic acid detection; signal amplification; telomerase
MeSH headings : Base Sequence; Cell Line; Chromatography, High Pressure Liquid; DNA Probes; Exodeoxyribonucleases / metabolism; Humans; Mass Spectrometry; Polymerase Chain Reaction / methods; Telomerase / metabolism
TL;DR: The sensitive detection of telomerase activity is reported by using exonuclease III-aided target recycling to amplify the signal produced by a chimeric LNA-DNA molecular beacon in a single-measurement fluorescence assay. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Crossref, ORCID
Added: July 11, 2021

2010 journal article

An Electrochemical Supersandwich Assay for Sensitive and Selective DNA Detection in Complex Matrices

Journal of the American Chemical Society, 132(41), 14346–14348.

By: F. Xia*, R. White*, X. Zuo*, A. Patterson*, Y. Xiao*, D. Kang*, X. Gong*, K. Plaxco*, A. Heeger*

MeSH headings : DNA / analysis; Electrochemistry / methods; Limit of Detection; Nucleic Acid Hybridization
TL;DR: This work employs a modified signal probe containing a methylene blue (a redox moiety) label and a "sticky end" to create a supersandwich structure containing multiple labels that leads to large signal amplification upon monitoring by voltammetry. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: August 3, 2021

2010 journal article

Colorimetric detection of DNA, small molecules, proteins, and ions using unmodified gold nanoparticles and conjugated polyelectrolytes

Proceedings of the National Academy of Sciences, 107(24), 10837–10841.

By: F. Xia*, X. Zuo*, R. Yang*, Y. Xiao*, D. Kang*, A. Vallee-Belisle*, X. Gong*, J. Yuen* ...

author keywords: biosensor; aptamer; visual detection; thrombin detection; cocaine detection
MeSH headings : Biosensing Techniques / methods; Cocaine / analysis; Colorimetry / methods; DNA / analysis; Fluorenes; Gold; Humans; Ions / analysis; Metal Nanoparticles; Polymers; Proteins / analysis; Quaternary Ammonium Compounds
TL;DR: A novel sensing strategy employing single-stranded probe DNA, unmodified gold nanoparticles, and a positively charged, water-soluble conjugated polyelectrolyte to detect a broad range of targets including nucleic acid sequences, proteins, small molecules, and inorganic ions is demonstrated. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: August 3, 2021

2010 journal article

Detection of Proteins in Serum by Micromagnetic Aptamer PCR (MAP) Technology

Angewandte Chemie International Edition, 49(2), 355–358.

By: A. Csordas*, A. Gerdon*, J. Adams*, J. Qian*, S. Oh*, Y. Xiao*, H. Soh*

author keywords: analytical methods; aptamers; growth factors; polymerase chain reaction; proteins
MeSH headings : Aptamers, Nucleotide / chemistry; Becaplermin; Biomarkers; Blood Proteins / chemistry; Calibration; DNA / chemistry; Electromagnetic Fields; Humans; Indicators and Reagents; Magnetics; Microfluidic Analytical Techniques; Platelet-Derived Growth Factor / analysis; Platelet-Derived Growth Factor / metabolism; Protein Array Analysis; Proto-Oncogene Proteins c-sis; Reproducibility of Results; Reverse Transcriptase Polymerase Chain Reaction / methods
TL;DR: The micromagnetic aptamer PCR (MAP) detection system is reported, which integrates high-gradient magnetic field sample preparation in a microfluidic device with aptamer-based real-time PCR readout, to achieve highly sensitive and quantitative detection of protein targets directly from complex samples. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: August 3, 2021

2010 journal article

Detection of Telomerase Activity in High Concentration of Cell Lysates Using Primer-Modified Gold Nanoparticles

Journal of the American Chemical Society, 132(43), 15299–15307.

By: Y. Xiao*, K. Dane*, T. Uzawa*, A. Csordas*, J. Qian*, H. Soh*, P. Daugherty*, E. Lagally*, A. Heeger*, K. Plaxco*

MeSH headings : Artifacts; Base Sequence; Cell Extracts; Cell Line, Tumor; Citric Acid / chemistry; DNA Primers / genetics; Gold / chemistry; Humans; Mammary Glands, Human / enzymology; Metal Nanoparticles / chemistry; Osmolar Concentration; Polymerase Chain Reaction / methods; Repetitive Sequences, Nucleic Acid; Telomerase / metabolism; Telomere / genetics; Time Factors
TL;DR: It appears that the use of AuNP-modified primers significantly improves the sensitivity and specificity of the traditional TRAP assay and may be an effective method by which PCR can be performed directly in concentrated cell lysates. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

2010 journal article

Electrochemical DNA Detection via Exonuclease and Target-Catalyzed Transformation of Surface-Bound Probes

Langmuir, 26(12), 10392–10396.

By: K. Hsieh, Y. Xiao* & H. Tom Soh*

MeSH headings : Catalysis; DNA / analysis; Electrochemical Techniques / methods; Electrochemical Techniques / standards; Exonucleases / metabolism; Molecular Probe Techniques; Molecular Probes
TL;DR: A single-step, single-reagent, label-free, isothermal electrochemical DNA sensor based on the phenomenon of target recycling that achieves an improved detection limit in comparison to hybridization-based sensors without amplification and demonstrates a 16-fold signal amplification factor at low target concentrations. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: August 3, 2021

2010 journal article

In vitro selection of structure-switching, self-reporting aptamers

Proceedings of the National Academy of Sciences, 107(32), 14053–14058.

By: S. Oh*, K. Plakos*, X. Lou*, Y. Xiao* & H. Soh*

author keywords: G-quadruplex; systematic evolution of ligands by exponential enrichment; sensor; binding induced folding; molecular recognition
MeSH headings : Aptamers, Nucleotide / chemistry; Aptamers, Nucleotide / isolation & purification; DNA, Catalytic / metabolism; Fluorescence; Fluorescent Dyes; Gene Library; Humans; Mesoporphyrins; Nucleic Acid Conformation; Thrombin / genetics
TL;DR: An innovative selection approach to generate self-reporting aptamers (SRAs) capable of converting target-binding events into fluorescence readout without requiring additional modification, optimization, or the use of DNA helper strands is described. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

2010 journal article

Label-Free, Dual-Analyte Electrochemical Biosensors: A New Class of Molecular-Electronic Logic Gates

Journal of the American Chemical Society, 132(25), 8557–8559.

By: F. Xia*, X. Zuo*, R. Yang*, R. White*, Y. Xiao*, D. Kang*, X. Gong*, A. Lubin* ...

MeSH headings : Biosensing Techniques / methods; Cocaine / analysis; Computers, Molecular; DNA, Bacterial / genetics; DNA, Complementary / analysis; DNA, Complementary / genetics; Electricity; Electrochemistry; Logic; Salmonella typhimurium; Transistors, Electronic
TL;DR: An "XOR" gate built using label-free, dual-analyte electrochemical sensors and the activation of this logic gate via changing concentrations of cocaine and the relevant cDNA as inputs are described. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

2010 journal article

On the Binding of Cationic, Water-Soluble Conjugated Polymers to DNA: Electrostatic and Hydrophobic Interactions

Journal of the American Chemical Society, 132(4), 1252–1254.

By: F. Xia, X. Zuo, R. Yang, Y. Xiao*, D. Kang, A. Vallée-Bélisle, X. Gong, A. Heeger, K. Plaxco

MeSH headings : Binding Sites; Cations / chemistry; DNA / chemistry; DNA, Single-Stranded / chemistry; Hydrophobic and Hydrophilic Interactions; Nucleic Acid Conformation; Polymers / chemistry; Static Electricity; Water / chemistry
TL;DR: Water-soluble, cationic conjugated polymer binds single-stranded DNA with higher affinity than it binds double-Stranded or otherwise "folded" DNA, which can be used to design a variety of biosensors. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Crossref, ORCID
Added: August 3, 2021

2010 journal article

Quantitative selection of DNA aptamers through microfluidic selection and high-throughput sequencing

Proceedings of the National Academy of Sciences, 107(35), 15373–15378.

By: M. Cho*, Y. Xiao*, J. Nie*, R. Stewart*, A. Csordas*, S. Oh*, J. Thomson*, H. Soh*

author keywords: high-throughput DNA sequencing; PDGF-BB; Quantitative Selection of Aptamers through Sequencing; MicroMagnetic Separation device
MeSH headings : Aptamers, Nucleotide / chemistry; Aptamers, Nucleotide / genetics; Aptamers, Nucleotide / metabolism; Base Sequence; Becaplermin; Binding, Competitive; Cloning, Molecular; DNA, Single-Stranded / chemistry; DNA, Single-Stranded / genetics; DNA, Single-Stranded / metabolism; Gene Library; Kinetics; Microfluidics / methods; Platelet-Derived Growth Factor / genetics; Platelet-Derived Growth Factor / metabolism; Protein Binding; Proto-Oncogene Proteins c-sis; Reproducibility of Results; SELEX Aptamer Technique / methods; Sequence Analysis, DNA / methods
TL;DR: The Quantitative Selection of Aptamers through Sequencing method tracks the copy number and enrichment-fold of more than 10 million individual sequences through multiple selection rounds, enabling the identification of high-affinity aptamers without the need for the pool to fully converge to a small number of sequences. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

2010 journal article

Sensitive and Selective Amplified Fluorescence DNA Detection Based on Exonuclease III-Aided Target Recycling

Journal of the American Chemical Society, 132(6), 1816–1818.

By: X. Zuo*, F. Xia*, Y. Xiao* & K. Plaxco*

MeSH headings : DNA / analysis; DNA / chemistry; DNA / metabolism; Exodeoxyribonucleases / metabolism; Fluorescent Dyes / chemistry; Models, Molecular; Molecular Probes / chemistry; Nucleic Acid Conformation; Nucleic Acid Hybridization
TL;DR: A nuclease-amplified DNA detection scheme in which exonuclease III is used to "recycle" target molecules, thus leading to greatly improved sensitivity relative to, for example, traditional molecular beacons without any significant restriction in the choice of target sequences. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

2010 report

Signal-on architecture for electronic oligonucleotide-based detectors

(Patent No. US7803542B2).

By: Y. Xiao, A. Lubin & K. Plaxco

Source: NC State University Libraries
Added: July 27, 2021

2009 journal article

An Electrochemical Sensor for Single Nucleotide Polymorphism Detection in Serum Based on a Triple-Stem DNA Probe

Journal of the American Chemical Society, 131(42), 15311–15316.

By: Y. Xiao*, X. Lou*, T. Uzawa*, K. Plakos*, K. Plaxco* & H. Soh*

MeSH headings : Animals; Biosensing Techniques; Cattle; DNA / blood; DNA / genetics; DNA Probes / analysis; DNA Probes / genetics; Electrochemical Techniques; Polymorphism, Single Nucleotide
TL;DR: An electrochemical approach that offers, for the first time, single-step, room-temperature single nucleotide polymorphism (SNP) detection directly in complex samples without the need for target modification, postwashing, or the addition of exogenous reagents is reported. (via Semantic Scholar)
UN Sustainable Development Goal Categories
10. Reduced Inequalities (OpenAlex)
Sources: Crossref, ORCID
Added: July 11, 2021

2009 journal article

Continuous, Real-Time Monitoring of Cocaine in Undiluted Blood Serum via a Microfluidic, Electrochemical Aptamer-Based Sensor

Journal of the American Chemical Society, 131(12), 4262–4266.

By: J. Swensen*, Y. Xiao*, B. Ferguson*, A. Lubin*, R. Lai*, A. Heeger*, K. Plaxco*, H. Soh*

MeSH headings : Aptamers, Nucleotide; Biosensing Techniques / instrumentation; Cocaine / analysis; Cocaine / blood; DNA / chemistry; Dose-Response Relationship, Drug; Electrochemistry / instrumentation; Electrochemistry / methods; Equipment Design; Humans; Microfluidic Analytical Techniques; Microfluidics / instrumentation; Optics and Photonics; Serum; Time Factors
TL;DR: The approach of integrating folding-based electrochemical sensors with miniaturized detection systems may lay the groundwork for the real-time, point-of-care detection of a wide variety of molecular targets. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Crossref, ORCID
Added: July 11, 2021

2009 chapter

Electrochemical Approaches to Aptamer-Based Sensing

In Functional Nucleic Acids for Analytical Applications (pp. 179–197).

By: Y. Xiao* & K. Plaxco*

TL;DR: The ability of electrode-bound aptamers to undergo reversible, binding-induced folding provides a robust, reagentless means of transducing target binding into an electronic signal that is largely impervious to nonspecific signals arising from contaminants. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

2009 journal article

Fluorescence Detection of Single-Nucleotide Polymorphisms with a Single, Self-Complementary, Triple-Stem DNA Probe

Angewandte Chemie International Edition, 48(24), 4354–4358.

By: Y. Xiao*, K. Plakos*, X. Lou*, R. White*, J. Qian*, K. Plaxco*, H. Soh*

author keywords: DNA; fluorescent probes; self-complementary sequences; single-nucleotide polymorphisms
MeSH headings : Base Sequence; DNA Probes / chemistry; DNA Probes / genetics; Fluorescent Dyes / chemistry; Polymorphism, Single Nucleotide; Spectrometry, Fluorescence; Thermodynamics
TL;DR: In a single-step, single-component, fluorescence-based method for the detection of single-nucleotide polymorphisms at room temperature, the sensor is comprised of a single, self-complementary DNA strand that forms a triple-stem structure. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: August 3, 2021

2009 journal article

Generation of Highly Specific Aptamers via Micromagnetic Selection

Analytical Chemistry, 81(13), 5490–5495.

By: J. Qian*, X. Lou*, Y. Zhang*, Y. Xiao* & H. Soh*

MeSH headings : Aptamers, Nucleotide / chemistry; Base Sequence; Magnetics; Microfluidics / methods; Molecular Sequence Data; SELEX Aptamer Technique / instrumentation; SELEX Aptamer Technique / methods; Streptavidin / analysis; Substrate Specificity
TL;DR: This paper presents here an alternative M-SELEX method, which employs a disposable microfluidic chip to rapidly generate aptamers with high affinity and specificity, and demonstrates exceptional separation efficiency in removing weakly bound and unbound ssDNA to rapidly enrich target-specific aptamer. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

2009 journal article

High Specificity, Electrochemical Sandwich Assays Based on Single Aptamer Sequences and Suitable for the Direct Detection of Small-Molecule Targets in Blood and Other Complex Matrices

Journal of the American Chemical Society, 131(20), 6944–6945.

By: X. Zuo*, Y. Xiao* & K. Plaxco*

MeSH headings : Adenosine Triphosphate / analysis; Adenosine Triphosphate / blood; Aptamers, Nucleotide / chemistry; Biosensing Techniques / methods; Blood Chemical Analysis / methods; Cocaine / analysis; Cocaine / blood; Electrochemical Techniques / methods; Sensitivity and Specificity
TL;DR: A sandwich assay based on single aptamer sequences is suitable for the direct detection of small molecule targets in blood serum and other complex matrices using single anticocaine and anti-ATP aptamers to fabricate electrochemical sensors directed against the representative small molecules cocaine and ATP. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

2009 journal article

Label-Free Colorimetric Screening of Nuclease Activity and Substrates by Using Unmodified Gold Nanoparticles

ChemBioChem, 10(12), 1973–1977.

author keywords: high-throughput screening; nanoparticles; nucleases; nucleoside monophosphates; substrate selectivity
MeSH headings : Colorimetry / methods; Endonucleases / chemistry; Endonucleases / isolation & purification; Endonucleases / metabolism; Gold / chemistry; Metal Nanoparticles / chemistry; Sensitivity and Specificity; Substrate Specificity; Surface Properties
TL;DR: A simple, straightforward and general colorimetric method to measure nuclease activity and screen substrate using unmodified AuNPs, namely, the ability to obtain results without a detector is described. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

2009 journal article

Micromagnetic selection of aptamers in microfluidic channels

Proceedings of the National Academy of Sciences, 106(9), 2989–2994.

By: X. Lou, J. Qian, Y. Xiao*, L. Viel, A. Gerdon, E. Lagally, P. Atzberger*, T. Tarasow*, A. Heeger, H. Soh

author keywords: microchannel; recombinant Botulinum neurotoxin type A; systematic evolution of ligands by exponential enrichment
MeSH headings : Aptamers, Nucleotide / chemistry; Aptamers, Nucleotide / isolation & purification; Databases, Nucleic Acid; Magnetics; Microfluidic Analytical Techniques / instrumentation; Microfluidic Analytical Techniques / methods; Nucleic Acid Conformation
TL;DR: An aptamer discovery system that is rapid, highly efficient, automatable, and applicable to a wide range of targets, based on the integration of magnetic bead-based SELEX process with microfluidics technology is described. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

2009 journal article

On the Signaling of Electrochemical Aptamer-Based Sensors: Collision- and Folding-Based Mechanisms

Electroanalysis, 21(11), 1267–1271.

By: Y. Xiao*, T. Uzawa*, R. White*, D. DeMartini* & K. Plaxco*

author keywords: Aptamer-based sensors; Folding and unfolding; Biosensors; Aptamers; Thrombin; IgE
TL;DR: It appears that binding-induced changes in the dynamics in fully folded aptamers can support E-AB signaling, but this signaling mechanism may not be general, and in order to ensure the design of high-gain sensors binding must be linked to a large-scale conformational change. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

2009 journal article

i-Motif Quadruplex DNA-Based Biosensor for Distinguishing Single- and Multiwalled Carbon Nanotubes

Journal of the American Chemical Society, 131(38), 13813–13818.

MeSH headings : Base Sequence; Biosensing Techniques; DNA / chemistry; G-Quadruplexes; Humans; Nanotubes, Carbon / chemistry; Telomere / chemistry
TL;DR: An electrochemical DNA (E-DNA) sensor that can distinguish single- and multiwalled carbon nanotubes both in buffer and in cell extracts is designed and demonstrated, which is the first demonstration of a biosensing technique that could distinguish different types of nanot tubes. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

2008 journal article

Optimization of Electrochemical Aptamer-Based Sensors via Optimization of Probe Packing Density and Surface Chemistry

Langmuir, 24(18), 10513–10518.

By: R. White*, N. Phares*, A. Lubin*, Y. Xiao* & K. Plaxco*

MeSH headings : Aptamers, Nucleotide / chemistry; Biosensing Techniques; Cocaine / chemistry; DNA / chemistry; Electrochemistry / methods; Electrodes; Equipment Design; Humans; Nucleic Acid Conformation; Oligonucleotides / chemistry; Oxidation-Reduction; RNA / chemistry; Surface Properties; Thrombin / chemistry
TL;DR: It is demonstrated that fabrication and operational parameters can be varied to achieve optimal sensor performance and that these can serve as a basic outline for future sensor fabrication. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

2008 journal article

Optimization of a Reusable, DNA Pseudoknot-Based Electrochemical Sensor for Sequence-Specific DNA Detection in Blood Serum

Analytical Chemistry, 81(2), 656–661.

By: K. Cash*, A. Heeger*, K. Plaxco* & Y. Xiao*

MeSH headings : Base Sequence; Biosensing Techniques; DNA / blood; DNA / chemistry; Electrochemistry; Electrodes; Gold / chemistry
TL;DR: Optimization over this parameter space significantly improves the signaling of the pseudoknot-based E-DNA architecture, leading to the ability to sensitively and specifically detect DNA targets even when challenged in complex, multicomponent samples such as blood serum. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

2007 report

Catalytic polynucleotide and its use for determination of analytes

(US Patent No. 20070254282).

By: I. Willner, Y. Xiao, V. Pavlov & T. Niazov

Source: NC State University Libraries
Added: September 11, 2021

2007 journal article

Electrochemical Detection of Parts-Per-Billion Lead via an Electrode-Bound DNAzyme Assembly

Journal of the American Chemical Society, 129(2), 262–263.

By: Y. Xiao*, A. Rowe* & K. Plaxco*

MeSH headings : Biosensing Techniques / methods; DNA, Catalytic / chemistry; Electrochemistry; Electrodes; Lead / analysis; Sensitivity and Specificity
TL;DR: An electrochemical lead sensor is based on lead-dependent changes in the conformational state of a surface-immobilized, methylene-blue modified DNAzyme assembly. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

2007 journal article

Label-Free Electrochemical Detection of DNA in Blood Serum via Target-Induced Resolution of an Electrode-Bound DNA Pseudoknot

Journal of the American Chemical Society, 129(39), 11896–11897.

By: Y. Xiao*, X. Qu*, K. Plaxco* & A. Heeger*

MeSH headings : DNA / blood; DNA Probes / chemistry; DNA, Single-Stranded / chemistry; Electrochemistry / methods; Electrodes; Gold / chemistry; Humans; Nucleic Acid Conformation; Polyethylene Glycols / chemistry; Sensitivity and Specificity
TL;DR: A fully covalent, signal-on E-DNA architecture based on the target-induced resolution of a DNA pseudokont is demonstrated, leading to the formation of a single-stranded DNA element that supports electron transfer from the methylene blue to the electrode. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

2007 journal article

Preparation of electrode-immobilized, redox-modified oligonucleotides for electrochemical DNA and aptamer-based sensing

Nature Protocols, 2(11), 2875–2880.

By: Y. Xiao*, R. Lai* & K. Plaxco*

MeSH headings : Aptamers, Nucleotide / analysis; Biosensing Techniques; DNA / analysis; DNA / chemistry; DNA Probes / chemistry; Electrochemistry; Electrodes; Nucleic Acid Conformation; Oligonucleotide Probes / chemistry; Oxidation-Reduction
TL;DR: This protocol describes the fabrication of E-DNA and E-AB sensors, which are comprised of an oligonucleotide probe modified with a redox reporter at one terminus and attached to a gold electrode via a thiol-gold bond at the other. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

2006 chapter

Catalytic Beacons for the Detection of DNA and Telomerase Activity

In D. Morrison, F. Milanovich, D. Ivnitski, & T. R. Austin (Eds.), Defense against Bioterror (pp. 199–205).

By: Y. Xiao*, V. Pavlov*, T. Niazov*, A. Dishon, M. Kotler & I. Willner*

Ed(s): D. Morrison, F. Milanovich, D. Ivnitski & T. Austin

Sources: Crossref, ORCID
Added: August 3, 2021

2006 report

Novel sensors with nanoparticle probe

(Patent Application No. WO2006008742).

By: I. Willner, V. Pavlov, M. Zayatz, T. Niazov, B. Shlyahovsky, Y. Xiao, R. Buron

Source: NC State University Libraries
Added: September 11, 2021

2006 journal article

Single-step electronic detection of femtomolar DNA by target-induced strand displacement in an electrode-bound duplex

Proceedings of the National Academy of Sciences, 103(45), 16677–16680.

By: Y. Xiao*, A. Lubin*, B. Baker*, K. Plaxco* & A. Heeger*

author keywords: biosensors; electron transfer; gold electrode; methylene blue; signal-on
MeSH headings : Base Sequence; Biosensing Techniques / instrumentation; Biosensing Techniques / methods; DNA / analysis; DNA / chemistry; DNA / genetics; Electrochemistry; Electrodes; Gold; Methylene Blue; Microchemistry; Molecular Probe Techniques; Quartz
TL;DR: A signal-on, electronic DNA (E-DNA) sensor that is label-free and achieves a subpicomolar detection limit and appears to provide a ready alternative to the more cumbersome femtomolar electrochemical assays described to date. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

2005 journal article

A Reagentless Signal-On Architecture for Electronic, Aptamer-Based Sensors via Target-Induced Strand Displacement

Journal of the American Chemical Society, 127(51), 17990–17991.

By: Y. Xiao*, B. Piorek*, K. Plaxco* & A. Heeger*

MeSH headings : Aptamers, Nucleotide / chemistry; Biosensing Techniques / methods; DNA / chemistry; Electrochemistry; Gold / chemistry; Thrombin / chemistry
TL;DR: Thrombin binding stabilizes the alternative G-quadruplex conformation of the aptamer, liberating the methylene blue (MB)-tagged oligonucleotide to produce a flexible, single-stranded DNA element. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

2005 journal article

An OsII-Bisbipyridine-4-Picolinic Acid Complex Mediates the Biocatalytic Growth of Au Nanoparticles: Optical Detection of Glucose and Acetylcholine Esterase Inhibition

Chemistry - A European Journal, 11(9), 2698–2704.

By: Y. Xiao*, V. Pavlov*, B. Shlyahovsky* & I. Willner*

author keywords: enzymes; gold; inhibitors; nanotechnology; sensors
MeSH headings : 2,2'-Dipyridyl / chemistry; Catalysis; Cholinesterase Inhibitors / analysis; Cholinesterase Inhibitors / chemistry; Glucose / analysis; Gold / chemistry; Nanostructures / chemistry; Organometallic Compounds / chemistry; Osmium / chemistry; Picolinic Acids / chemistry; Spectrophotometry, Ultraviolet
TL;DR: The complex Os(II)-bisbipyridine-4-picolinic acid, [Os(bpy)(2)PyCO(2)H](2+) mediates the biocatalyzed growth of Au nanoparticles, Au NPs, and enables the spectroscopic assay of bioc atalyzed transformations and enzyme inhibition by following the Au NP plasmon absorbance. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

2005 journal article

Inhibition of the Acetycholine Esterase-Stimulated Growth of Au Nanoparticles:  Nanotechnology-Based Sensing of Nerve Gases

Nano Letters, 5(4), 649–653.

By: V. Pavlov*, Y. Xiao* & I. Willner*

MeSH headings : Acetylcholinesterase / chemistry; Acetylcholinesterase / drug effects; Biosensing Techniques; Chemical Warfare Agents / analysis; Cholinesterase Inhibitors / pharmacology; Gold / chemistry; Nanostructures / chemistry; Nanotechnology / methods; Oxidation-Reduction; Paraoxon / pharmacology
TL;DR: The catalytic growth of the Au NPs is inhibited by 1,5-bis(4-allyldimethylammoniumphenyl)pentane-3-one dibromide or by diethyl p-nitrophenyl phosphate (paraoxon; 4), thus enabling a colorimetric test for AChE inhibitors. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

2005 journal article

Label-Free Electronic Detection of Thrombin in Blood Serum by Using an Aptamer-Based Sensor

Angewandte Chemie International Edition, 44(34), 5456–5459.

By: Y. Xiao*, A. Lubin*, A. Heeger* & K. Plaxco*

author keywords: aptamers; biosensors; DNA structures; electron transfer; thrombin
MeSH headings : Aptamers, Nucleotide / chemistry; Biosensing Techniques / instrumentation; Biosensing Techniques / methods; Electrochemistry; Humans; Models, Biological; Sensitivity and Specificity; Thrombin / analysis
Sources: Crossref, ORCID
Added: August 3, 2021

2005 journal article

Optical and Electrochemical Detection of NADH and of NAD+-Dependent Biocatalyzed Processes by the Catalytic Deposition of Copper on Gold Nanoparticles

Small, 1(2), 213–216.

By: B. Shlyahovsky*, E. Katz*, Y. Xiao*, V. Pavlov* & I. Willner*

author keywords: copper; enzymes; gold; nanoparticles; sensors
MeSH headings : Biosensing Techniques; Catalysis; Copper / chemistry; Electrochemistry / methods; Electrodes; Gold / chemistry; Metal Nanoparticles / chemistry; Microscopy, Electron, Scanning; NAD / chemistry; Nanotechnology / methods; Propylamines; Silanes / chemistry; Surface Properties; Temperature
Sources: Crossref, ORCID
Added: July 11, 2021

2005 journal article

Shape and color of Au nanoparticles follow biocatalytic processes

Langmuir, 21(13), 5659–5662.

By: Y. Xiao*, B. Shlyahovsky*, I. Popov*, V. Pavlov* & I. Willner*

MeSH headings : Ascorbic Acid; Catalysis; Crystallography; Gold Compounds; Microscopy, Electron; Models, Molecular; NAD / chemistry; NADP / chemistry; Nanostructures / chemistry; Oxidation-Reduction
TL;DR: The NAD(P)H-mediated growth of Au nanoparticles in the presence of ascorbic acid, AuCl4-, and cetyltrimethylammonium bromide leads to the formation of shaped NP structures consisting of dipods, tripods, and tetrapods, which provide a colorimetric test for ethanol. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 7, 2021

2004 journal article

Amplified Chemiluminescence Surface Detection of DNA and Telomerase Activity Using Catalytic Nucleic Acid Labels

Analytical Chemistry, 76(7), 2152–2156.

By: V. Pavlov*, Y. Xiao*, R. Gill*, A. Dishon*, M. Kotler* & I. Willner*

MeSH headings : Base Sequence; DNA / analysis; DNA / chemistry; DNA / metabolism; DNA, Catalytic / chemistry; Gold / pharmacology; HeLa Cells; Hemin / metabolism; Humans; Luminescence; Luminescent Measurements; Molecular Sequence Data; Photoaffinity Labels / chemistry; Surface Properties; Telomerase / analysis; Telomerase / metabolism
TL;DR: The DNAzyme is used as a label for the amplified detection of DNA, or for the analysis of telomerase activity in cancer cells, using chemiluminescence as an output signal. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

2004 journal article

Aptamer-Functionalized Au Nanoparticles for the Amplified Optical Detection of Thrombin

Journal of the American Chemical Society, 126(38), 11768–11769.

By: V. Pavlov*, Y. Xiao*, B. Shlyahovsky* & I. Willner*

MeSH headings : Animals; Gold / chemistry; Humans; Immunoglobulin G / analysis; Immunoglobulin G / chemistry; Nanostructures / chemistry; Oligonucleotides / chemistry; Optics and Photonics; Sensitivity and Specificity; Serum Albumin / analysis; Serum Albumin / chemistry; Spectrum Analysis / methods; Thrombin / analysis
TL;DR: The catalytic enlargement of aptamer-functionalized Au nanoparticles amplifies the optical detection ofaptamer-thrombin complexes in solution and on surfaces. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

2004 journal article

Catalytic Beacons for the Detection of DNA and Telomerase Activity

Journal of the American Chemical Society, 126(24), 7430–7431.

By: Y. Xiao*, V. Pavlov*, T. Niazov*, A. Dishon*, M. Kotler* & I. Willner*

MeSH headings : Catalysis; Cell Count; DNA / analysis; DNA / metabolism; DNA, Catalytic / chemistry; DNA, Catalytic / metabolism; Enzyme Activation; HeLa Cells; Humans; Molecular Probe Techniques; Nucleic Acid Conformation; Nucleic Acid Hybridization; Nucleic Acids / chemistry; Photochemistry; Sensitivity and Specificity; Telomerase / analysis; Telomerase / chemistry; Telomerase / metabolism; Time Factors
TL;DR: DNA and telomerase activity is detected by a DNAzyme generated upon hybridization and opening of a functional catalytic beacon. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

2004 journal article

Catalytic Growth of Au Nanoparticles by NAD(P)H Cofactors: Optical Sensors for NAD(P)+-Dependent Biocatalyzed Transformations

Angewandte Chemie International Edition, 43(34), 4519–4522.

By: Y. Xiao*, V. Pavlov*, S. Levine*, T. Niazov*, G. Markovitch* & I. Willner*

author keywords: biosensors; cofactors; enzymes; gold; nanostructures
MeSH headings : Biosensing Techniques / methods; Catalysis; Gold / chemistry; NAD / chemistry; NADP / chemistry; Nanostructures; Oxidation-Reduction; Particle Size; Quartz
Sources: Crossref, ORCID
Added: July 11, 2021

2004 journal article

DNAzyme-Functionalized Au Nanoparticles for the Amplified Detection of DNA or Telomerase Activity

Nano Letters, 4(9), 1683–1687.

By: T. Niazov*, V. Pavlov*, Y. Xiao*, R. Gill* & I. Willner*

TL;DR: The method enables the detection of telomerase activity originating from 1000 HeLa cells and generates chemiluminescence in the presence of hemin, H2O2, and luminol. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

2004 journal article

Electrical contacting of glucose oxidase by DNA-templated polyaniline wires on surfaces

Electrochemistry Communications, 6(10), 1057–1060.

By: L. Shi*, Y. Xiao* & I. Willner*

author keywords: poly(aniline-aniline boronic acid) wires; DNA template; apo-glucose oxidase; reconstitution; biosensor
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Crossref, ORCID
Added: July 11, 2021

2004 journal article

Lighting Up Biochemiluminescence by the Surface Self-Assembly of DNA-Hemin Complexes

ChemBioChem, 5(3), 374–379.

By: Y. Xiao*, V. Pavlov*, R. Gill*, T. Bourenko* & I. Willner*

author keywords: biocatalysis; DNA; hemin; luminescence; monolayers; self-assembly
MeSH headings : Base Sequence; DNA, Catalytic / chemistry; Enzymes, Immobilized; Hemin / chemistry; Hydrogen Peroxide; Luminescent Measurements; Luminol; Nucleic Acid Conformation
TL;DR: It is reported that two separated nucleic acids that include the segments A and B–constituting the single-stranded peroxidase deoxyribozyme forms a layered G-quadruplex structure that forms a biocatalytic and bioelectrocatalytic formation of chemiluminescence in the presence of H2O2 and luminol. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: August 3, 2021

2003 journal article

"Plugging into Enzymes": Nanowiring of Redox Enzymes by a Gold Nanoparticle

Science, 299(5614), 1877–1881.

By: Y. Xiao*

MeSH headings : Apoenzymes / chemistry; Apoenzymes / metabolism; Ascorbic Acid / metabolism; Ascorbic Acid / pharmacology; Biosensing Techniques; Catalysis; Crystallization; Electrochemistry; Electrodes; Electrons; Flavin-Adenine Dinucleotide / chemistry; Glucose / metabolism; Glucose Oxidase / chemistry; Glucose Oxidase / metabolism; Gold; Nanotechnology; Oxidation-Reduction; Oxygen / metabolism
TL;DR: The reconstitution of an apo-flavoenzyme on a 1.4-nanometer gold nanocrystal functionalized with the cofactor flavin adenine dinucleotide and integrated into a conductive film yields a bioelectrocatalytic system with exceptional electrical contact with the electrode support. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

2003 journal article

Electrocatalytic intercalator-induced winding of double-stranded DNA with polyaniline

Chemical Communications, (13), 1540.

By: Y. Xiao*, A. Kharitonov*, F. Patolsky*, Y. Weizmann* & I. Willner*

MeSH headings : Aniline Compounds / chemistry; DNA / chemistry; DNA, Catalytic / chemistry; Electrodes
TL;DR: The intercalation of doxorubicin into double-stranded DNA stimulates the electocatalyzed oxidation of aniline to polyaniline and its winding on the DNA template. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

2002 journal article

Electrooxidative coupling of a toluidine blue O terminated self-assembled monolayer studied by electrochemistry and surface enhanced Raman spectroscopy

Journal of Electroanalytical Chemistry, 518(2), 123–130.

By: H. Ju*, Y. Xiao*, X. Lu* & H. Chen*

author keywords: chemically modified electrode; surface enhanced Raman spectroscopy; electropolymerization; monolayers; toluidine blue O; electrooxidative coupling
Sources: Crossref, ORCID
Added: July 11, 2021

2001 journal article

Anion adsorption on an Au colloid monolayer based cysteamine-modified gold electrode

Chemical Research in Chinese Universities, 17(2), 159–167.

By: X. Hu, Y. Xiao & H. Chen

Source: NC State University Libraries
Added: September 11, 2021

2000 journal article

Direct Electrochemistry of Horseradish Peroxidase Immobilized on a Colloid/Cysteamine-Modified Gold Electrode

Analytical Biochemistry, 278(1), 22–28.

author keywords: horseradish peroxidase; direct electrochemistry; Au colloid; cysteamine; biosensor; self-assembled monolayers
MeSH headings : Biosensing Techniques / instrumentation; Colloids; Cysteamine; Electrochemistry; Electrodes; Electron Transport; Enzymes, Immobilized; Gold; Horseradish Peroxidase / metabolism; Hydrogen Peroxide / analysis
TL;DR: Direct electron transfer of immobilized horseradish peroxidase on gold colloid and its application as a biosensor showed an excellent electrocatalytic response to the reduction of H( 2)O(2) without the aid of an electron mediator and showed a Michaelis-Menten behavior at higher H(2). (via Semantic Scholar)
Sources: Crossref, ORCID
Added: August 3, 2021

1999 journal article

A reagentless hydrogen peroxide sensor based on incorporation of horseradish peroxidase in poly(thionine) film on a monolayer modified electrode

Analytica Chimica Acta, 391(3), 299–306.

By: Y. Xiao*, H. Ju* & H. Chen*

author keywords: hydrogen peroxide sensor; poly(thionine) film; coimmobilization; horseradish peroxidase; thionine monolayer; gold electrode
Sources: Crossref, ORCID
Added: July 11, 2021

1999 journal article

Adsorption characteristics of Fe(CN)63−/4− on Au colloids as monolayer films on cysteamine-modified gold electrode

Journal of Electroanalytical Chemistry, 466(1), 26–30.

By: X. Hu*, Y. Xiao* & H. Chen*

author keywords: colloidal gold; self-assembled monolayers; gold electrode
Sources: Crossref, ORCID
Added: July 11, 2021

1999 journal article

Hydrogen peroxide sensor based on horseradish peroxidase-labeled Au colloids immobilized on gold electrode surface by cysteamine monolayer

Analytica Chimica Acta, 391(1), 73–82.

By: Y. Xiao*, H. Ju* & H. Chen*

author keywords: self-assembled monolayers; HRP-labeled Au colloids; cysteamine; hydrogen peroxide; sensors; electrochemical sensors
TL;DR: The low K app M values demonstrated that Horseradish peroxidase immobilized on Au colloids exhibited a high affinity to H 2 O 2 with no loss of enzymatic activity. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: July 11, 2021

1998 journal article

Amperometric Biosensor for Glucose Based on a Nanometer-Sized Microband Gold Electrode Coimmobilized with Glucose Oxidase and Poly(o-phenylenediamide)

Electroanalysis, 10(8), 541–545.

By: H. Ju, D. Zhou, Y. Xiao* & H. Chen

author keywords: glucose sensors; nanometer-sized biosensors; o-phenylenediamide; glucose oxidase; electrochemical polymerization; microband gold electrodes
Sources: Crossref, ORCID
Added: July 11, 2021

Employment

Updated: October 26th, 2021 22:06

2021 - present

North Carolina State University Raleigh, NC, US
Department of Chemistry

2011 - 2021

Florida International University MIAMI, FL, US
Associate Professor Chemistry and Biochemistry

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