Yi Xiao

DNA Aptamer-Cyanine Complexes as Generic Colorimetric Small-Molecule Sensors

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 61(3).

High-throughput quantitative binding analysis of DNA aptamers using exonucleases

Canoura, J., Alkhamis, O., Liu, Y., Willis, C., & Xiao, Y. (2022, December 30). NUCLEIC ACIDS RESEARCH, Vol. 12.

Near-Infrared Dye-Aptamer Assay for Small Molecule Detection in Complex Specimens

Jin, X., Liu, Y., Alkhamis, O., Canoura, J., Bacon, A., Xu, R., … Xiao, Y. (2022, July 7). ANALYTICAL CHEMISTRY, Vol. 7.

Systematic Study of in Vitro Selection Stringency Reveals How To Enrich High-Affinity Aptamers

Alkhamis, O., & Xiao, Y. (2022, December 27). JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, Vol. 12.

Advances and Challenges in Small‐Molecule DNA Aptamer Isolation, Characterization, and Sensor Development

Angewandte Chemie International Edition, 60(31), 16800–16923.

Aptamer-Integrated Multianalyte-Detecting Paper Electrochemical Device

ACS Applied Materials & Interfaces, 13(15), 17330–17339.

Aptamers that bind to natural and synthetic cannabinoids

(Patent No. US10907163B1).

Development of Aptamer-Based Sensors for Sensitive and Specific Detection of Fentanyl Opioids

Presented at the Pittcon Conference and Expo.

Immobilization Strategies for Enhancing Sensitivity of Electrochemical Aptamer-Based Sensors

ACS Applied Materials & Interfaces, 13(8), 9491–9499.

Isolation of Natural DNA Aptamers for Challenging Small-Molecule Targets, Cannabinoids

Analytical Chemistry, 93(6), 3172–3180.

Materials and methods for colorimetric detection of small molecule targets

(Patent No. US10948504B2).

Method for isolating cross-reactive aptamer and use thereof

(Patent No. US10907162B2).

Platinum-Nanoparticle-Modified Single-Walled Carbon Nanotube-Laden Paper Electrodes for Electrocatalytic Oxidation of Methanol

Paudyal, J., Wang, P., Zhou, F., Liu, Y., Cai, Y., & Xiao, Y. (2021, December 15). ACS APPLIED NANO MATERIALS, Vol. 12.

Accelerating Post-SELEX Aptamer Engineering Using Exonuclease Digestion

Journal of the American Chemical Society, 143(2), 805–816.

Achieving Near-ideal Binding Spectra using Subideal Bioreceptors

Presented at the Pittcon 2020, Chicago, Illinois.

Beauty Through Simplicity – Problem-Driven Aptamer Science

Invited talk presented at the NC State University, Raleigh, NC.

Fabrication of Aptamer‐Modified Paper Electrochemical Devices for On‐Site Biosensing

Angewandte Chemie International Edition, 60(6), 2993–3000.

Isolation of Class-specific Aptamers and Development of Sensors for Instantaneous Detection of Synthetic Cathinones

Presented at the Pittcon, Chicago, IL.

Label-free profiling of DNA aptamer-small molecule binding using T5 exonuclease

Nucleic Acids Research, 48(20), e120–e120.

Materials and methods for colorimetric detection of small molecule targets

(Patent No. US10725058B2).

Materials and methods for rapid and sensitive detection of small-molecule targets

(Patent No. US10550395B2).

Materials and methods for rapid and specific detection of synthetic cathinones

(Patent No. US10683507B2).

Method for isolating cross-reactive aptamer and use thereof

(Patent No. US10655132B1).

Screening of aptamer mutants for enhanced target specificity using a dual exonuclease digestion assay

Presented at the Pittcon 2020, Chicago, Illinois.

Tuning Biosensor Cross-Reactivity Using Aptamer Mixtures

Analytical Chemistry, 92(7), 5041–5047.

A Cooperative-Binding Split Aptamer Assay for Rapid, Specific and Ultra-Sensitive Fluorescence Detection of Cocaine in Saliva

Presented at the Pittcon 2019, Philadelphia, Pennsylvania.

Beauty Through Simplicity – Functionalized Aptamers for On-Site Small-Molecule Detection

Presented at the Pittcon 2019, Philadelphia, Pennsylvania.

Development of Electrochemical Aptamer-Based Sensors for On-Site Synthetic Cathinone Detection

Presented at the Pittcon 2019, Philadelphia, Pennsylvanis.

Highly conductive porous paper-based metal thin films

(Patent No. US10290386B2).

In Vitro Isolation of Small-Molecule-Binding Aptamers with Intrinsic Dye-Displacement Functionality

Presented at the Pittcon 2019, Philadelphia, Pennsylvania.

In vitro isolation of class-specific oligonucleotide-based small-molecule receptors

Nucleic Acids Research, 47(12), e71–e71.

Innovative engineering and sensing strategies for aptamer-based small-molecule detection

TrAC Trends in Analytical Chemistry, 121, 115699.

Introducing Structure-Switching Functionality into Small-Molecule-Binding Aptamers via Nuclease-Directed Truncation

Presented at the Pittcon 2019, Philadelphia, Pennsylvania.

Label-Free, Visual Detection of Small Molecules Using Highly Target-Responsive Multimodule Split Aptamer Constructs

Analytical Chemistry, 91(11), 7199–7207.

Perspective on the Future Role of Aptamers in Analytical Chemistry

Analytical Chemistry, 91(24), 15335–15344.

Rapid, naked-eye screening for synthetic cathinones based on cross-reactive aptamers

Presented at the Pittcon 2019, Philadelphia, Pennsylvania.

Universal Design of Structure-Switching Aptamers with Signal Reporting Functionality

Analytical Chemistry, 91(22), 14514–14521.

Aptamer-based Assays for On-site Drug Detection

Presented at the Pittcon 2018, Orlando, Florida.

Beauty through simplicity – functionalized aptamers for on-site small-molecule detection

Invited talk presented at the University of Notre Dame, South Bend, Indiana.

Dithiothreitol-Regulated Coverage of Oligonucleotide-Modified Gold Nanoparticles To Achieve Optimized Biosensor Performance

ACS Applied Materials & Interfaces, 10(4), 4233–4242.

In vitro isolation of small-molecule-binding aptamers with intrinsic dye-displacement functionality

Nucleic Acids Research, 46(8), e43–e43.

Introducing structure-switching functionality into small-molecule-binding aptamers via nuclease-directed truncation

Nucleic Acids Research, 46(13), e81–e81.

Materials and methods for rapid visualization of NAD(P)H

(Patent No. US10000790B2).

No Structure-Switching Required: A Generalizable Exonuclease-Mediated Aptamer-Based Assay for Small-Molecule Detection

Journal of the American Chemical Society, 140(31), 9961–9971.

Rapid and sensitive detection of small-molecule targets via enzyme-assisted target recycling based on cooperative binding split aptamers

Presented at the Pittcon 2018, Orlando, Florida.

Sensitive Detection of Small-Molecule Targets Using Cooperative Binding Split Aptamers and Enzyme-Assisted Target Recycling

Analytical Chemistry, 90(3), 1748–1758.

Utilizing nuclease screening of ligand-aptamer complexes to enhance specificity of an aptamer-based cocaine assay

Presented at the 2017 Society of Forensic Toxicologists-International Association of Forensic Toxicologists (SOFT-TIAFT) Joint Meeting, Boca Raton, Florida.

A Broadly Applicable Assay for Rapidly and Accurately Quantifying DNA Surface Coverage on Diverse Particles

Bioconjugate Chemistry, 28(4), 933–943.

A cooperative-binding split aptamer assay for rapid, specific and ultra-sensitive fluorescence detection of cocaine in saliva

Poster presented at the Gordon Research Conference: Nucleosides, Nucleotides & Oligonucleotides, Salve Regina University, Newport, RI.

A cooperative-binding split aptamer assay for rapid, specific and ultra-sensitive fluorescence detection of cocaine in saliva

Chemical Science, 8(1), 131–141.

A label-free aptamer-fluorophore assembly for highly sensitive and specific detection of cocaine

Presented at the Pittcon 2017, Chicago, Illinois.

Cooperative-binding split aptamer assays for rapid, specific and ultra-sensitive detection of small-molecule targets in biofluid samples

Invited talk presented at the 2017 American Chemical Society Florida Annual Meeting and Exposition, Tampa, FL.

Gold nanoparticle-based assays for rapid and sensitive colorimetric and electrochemical biosensing

Presented at the Nano Florida 2017, Florida International University, Miami, FL.

Gold nanoparticle-based, paper devices for rapid and sensitive colorimetric and electrochemical biosensing

Invited talk presented at the 2017 American Chemical Society Florida Annual Meeting and Exposition, Tampa, FL.

Materials and methods for rapid and specific detection of cocaine

(Patent No. US9804178B2).

Rapid and sensitive detection of small-molecule targets via enzyme-assisted target recycling based on cooperative binding split aptamers

Poster presented at the Gordon Research Conference: Nucleosides, Nucleotides & Oligonucleotides, Salve Regina University, Newport, RI.

Utilizing dual-enzyme selectivity for the identification of aptamer binding domains and the development of sensitive fluorescence detection of small-molecule targets

Presented at the FIU Annual Scholarly Forum, Graduate Student Appreciation Week (GSAW), Florida International University, Miami, FL.

Utilizing nuclease screening of ligand-aptamer complexes to enhance specificity of an aptamer-based cocaine assay

Poster presented at the Gordon Research Conference: Nucleosides, Nucleotides & Oligonucleotides, Salve Regina University, Newport, RI.

A cooperative-binding split aptamer assay for rapid, specific and ultra-sensitive fluorescence detection of cocaine in saliva

Presented at the Pittcon 2017, Chicago, Illinois.

A cooperative-binding split aptamer assay for rapid, specific and ultra-sensitive fluorescence detection of cocaine in saliva

Presented at the 2016 Conference of Undergraduate Research at FIU (CURFIU), Miami, FL.

A cooperative-binding split aptamer assay for rapid, specific and ultra-sensitive fluorescence detection of cocaine in saliva

Presented at the FIU Annual Scholarly Forum, Graduate Student Appreciation Week (GSAW), Florida International University, Miami, FL.

A label-free aptamer-fluorophore assembly for highly sensitive and specific detection of cocaine

Presented at the 2016 Conference of Undergraduate Research at FIU (CURFIU), Miami, FL.

Ambient filtration method to rapidly prepare highly conductive, paper-based porous gold films for electrochemical biosensing

Presented at the 2016 Conference of Undergraduate Research at FIU (CURFIU), Miami, FL.

Bringing biosensors to the bedside

Invited talk presented at the University of Miami, Medical Campus - Batchelor Children's Research Institute, Miami, FL.

Paper-based device for rapid visualization of NADH based on dissolution of gold nanoparticles

Poster presented at the 2016 Conference of Undergraduate Research at FIU (CURFIU), Miami, FL.

Paper-based devices for rapid and sensitive colorimetric and electrochemical biosensing

Invited talk presented at the 2016 ACS Florida Annual Meeting and Exposition, Tampa, FL.

Rapid, Surfactant-Free, and Quantitative Functionalization of Gold Nanoparticles with Thiolated DNA under Physiological pH and Its Application in Molecular Beacon-Based Biosensor

ACS Applied Materials & Interfaces, 8(40), 27298–27304.

Utilizing nuclease screening of ligand-aptamer complexes to enhance specificity of an aptamer-based cocaine assay

Poster presented at the 2016 ACS Florida Annual Meeting and Exposition, Tampa, FL.

A paper-based device for rapid visualization of NADH based on dissolution of gold nanoparticles

Poster presented at the 2015 Materials Research Society (MRS) Fall Meeting & Exhibit, Boston, Massachusetts.

Ambient Filtration Method To Rapidly Prepare Highly Conductive, Paper-Based Porous Gold Films for Electrochemical Biosensing

ACS Applied Materials & Interfaces, 7(49), 27049–27058.

Ambient filtration method to rapidly prepare highly conductive, paper-based porous gold films

Presented at the 2015 Materials Research Society (MRS) Fall Meeting & Exhibit, Boston, Massachusetts.

Amplified colorimetric detection of cocaine in oral fluid based on exonuclease-assisted aptamer strand recycling

Poster presented at the Society of Forensic Toxicologists (SOFT) 2015 Annual Meeting, Atlanta, Georgia.

Aptamer-based, exonuclease-amplified, paper device for point of collection screening of cocaine and methamphetamine in oral fluid

Invited talk presented at the Forensic Science R&D Grantees Meeting, Orlando, FL.

Base-pair mismatch detection

Invited talk presented at the FIU Biomolecular Sciences Institute Symposium on DNA Repair and Genome Stability: In honor of the 2015 Nobel Prize in Chemistry for DNA Repair, Miami, FL.

Bringing biosensors to the bedside

Recruitment talk presented at the Division of Health and Natural Sciences, Florida Memorial University, Miami, FL.

Nanoprobe-Enhanced, Split Aptamer-Based Electrochemical Sandwich Assay for Ultrasensitive Detection of Small Molecules

Analytical Chemistry, 87(15), 7712–7719.

Paper-Based Device for Rapid Visualization of NADH Based on Dissolution of Gold Nanoparticles

ACS Applied Materials & Interfaces, 7(27), 15023–15030.

Simple, rapid and ultra-sensitive fluorescence detection of cocaine in oral fluid based on cooperative binding split aptamer

Presented at the Society of Forensic Toxicologists (SOFT) 2015 Annual Meeting, Atlanta, Georgia.

A Label-Free Aptamer-Fluorophore Assembly for Rapid and Specific Detection of Cocaine in Biofluids

Analytical Chemistry, 86(22), 11100–11106.

A label-free aptamer-fluorophore assembly for highly sensitive and specific detection of cocaine

Poster presented at the 2014 Materials Research Society (MRS) Fall Meeting & Exhibit, Boston, Massachusetts.

Amplified Single Base-Pair Mismatch Detection via Aggregation of Exonuclease-Sheared Gold Nanoparticles

Analytical Chemistry, 86(7), 3461–3467.

Amplified single base-pair mismatch detection via the aggregation of exonuclease-sheared gold nanoparticles

Poster presented at the 2014 Materials Research Society (MRS) Fall Meeting & Exhibit, Boston, Massachusetts.

Bringing biosensors to the bedside

Recruitment talk presented at the Department of Chemistry, St. Thomas University, Miami, FL.

Target recycling-based assay for rapid and accurate determination of DNA surface coverage on gold nanoparticles

Presented at the 2014 Materials Research Society (MRS) Fall Meeting & Exhibit, Boston, Massachusetts.

Ambient filtration method to prepare paper-based electroactive nanoporous gold thin film for detection of dopamine and serotonin

Presented at the 89th Florida Annual Meeting and Exposition, Palm Harbor, FL.

Bringing biosensors to the bedside

Recruitment talk presented at the Department of Chemistry, University of Tampa, Tampa, FL.

Controlling the function of DNA nanostructures with specific trigger sequences

Chem. Commun., 49(4), 397–399.

In Vitro Selection of Shape-Changing DNA Nanostructures Capable of Binding-Induced Cargo Release

ACS Nano, 7(11), 9675–9683.

Paper-based single-walled carbon nanotube thin film for catalytic detection of nicotinamide adenine dinucleotide in human blood

Invited talk presented at the 89th Florida Annual Meeting and Exposition, Palm Harbor, FL.

Self-Assembled DNA Monolayer Buffered Dynamic Ranges of Mercuric Electrochemical Sensor

Analytical Chemistry, 85(15), 7574–7580.

Biosensors, biotechnologies and biomaterials

Presented at the 9th International Symposium on Persistent Toxic Substances, Miami, FL.

Electrochemical DNA three-way junction based sensor for distinguishing chiral metallo-supramolecular complexes

Chemical Communications, 48(55), 6900.

Selection is more intelligent than design: improving the affinity of a bivalent ligand through directed evolution

Nucleic Acids Research, 40(22), 11777–11783.

Bringing biosensors to the bedside

Recruitment talk presented at the Department of Chemistry, Barry University, Miami, FL.

Genetic Analysis of H1N1 Influenza Virus from Throat Swab Samples in a Microfluidic System for Point-of-Care Diagnostics

Journal of the American Chemical Society, 133(23), 9129–9135.

Improving Aptamer Selection Efficiency through Volume Dilution, Magnetic Concentration, and Continuous Washing in Microfluidic Channels

Analytical Chemistry, 83(17), 6883–6889.

Measurement of Aptamer–Protein Interactions with Back-Scattering Interferometry

Analytical Chemistry, 83(23), 8867–8870.

Polarity-Switching Electrochemical Sensor for Specific Detection of Single-Nucleotide Mismatches

Angewandte Chemie International Edition, 50(47), 11176–11180.

Probing the Limits of Aptamer Affinity with a Microfluidic SELEX Platform

PLoS ONE, 6(11), e27051.

Selection of phage-displayed peptides on live adherent cells in microfluidic channels

Proceedings of the National Academy of Sciences, 108(17), 6909–6914.

Two-Step, PCR-Free Telomerase Detection by Using Exonuclease III-Aided Target Recycling

ChemBioChem, 12(18), 2745–2747.

An Electrochemical Supersandwich Assay for Sensitive and Selective DNA Detection in Complex Matrices

Journal of the American Chemical Society, 132(41), 14346–14348.

Colorimetric detection of DNA, small molecules, proteins, and ions using unmodified gold nanoparticles and conjugated polyelectrolytes

Proceedings of the National Academy of Sciences, 107(24), 10837–10841.

Detection of Proteins in Serum by Micromagnetic Aptamer PCR (MAP) Technology

Angewandte Chemie International Edition, 49(2), 355–358.

Detection of Telomerase Activity in High Concentration of Cell Lysates Using Primer-Modified Gold Nanoparticles

Journal of the American Chemical Society, 132(43), 15299–15307.

Electrochemical DNA Detection via Exonuclease and Target-Catalyzed Transformation of Surface-Bound Probes

Langmuir, 26(12), 10392–10396.

In vitro selection of structure-switching, self-reporting aptamers

Proceedings of the National Academy of Sciences, 107(32), 14053–14058.

Label-Free, Dual-Analyte Electrochemical Biosensors: A New Class of Molecular-Electronic Logic Gates

Journal of the American Chemical Society, 132(25), 8557–8559.

On the Binding of Cationic, Water-Soluble Conjugated Polymers to DNA: Electrostatic and Hydrophobic Interactions

Journal of the American Chemical Society, 132(4), 1252–1254.

Quantitative selection of DNA aptamers through microfluidic selection and high-throughput sequencing

Proceedings of the National Academy of Sciences, 107(35), 15373–15378.

Sensitive and Selective Amplified Fluorescence DNA Detection Based on Exonuclease III-Aided Target Recycling

Journal of the American Chemical Society, 132(6), 1816–1818.

Signal-on architecture for electronic oligonucleotide-based detectors

(Patent No. US7803542B2).

An Electrochemical Sensor for Single Nucleotide Polymorphism Detection in Serum Based on a Triple-Stem DNA Probe

Journal of the American Chemical Society, 131(42), 15311–15316.

Continuous, Real-Time Monitoring of Cocaine in Undiluted Blood Serum via a Microfluidic, Electrochemical Aptamer-Based Sensor

Journal of the American Chemical Society, 131(12), 4262–4266.

Electrochemical Approaches to Aptamer-Based Sensing

In Functional Nucleic Acids for Analytical Applications (pp. 179–197).

Fluorescence Detection of Single-Nucleotide Polymorphisms with a Single, Self-Complementary, Triple-Stem DNA Probe

Angewandte Chemie International Edition, 48(24), 4354–4358.

Generation of Highly Specific Aptamers via Micromagnetic Selection

Analytical Chemistry, 81(13), 5490–5495.

High Specificity, Electrochemical Sandwich Assays Based on Single Aptamer Sequences and Suitable for the Direct Detection of Small-Molecule Targets in Blood and Other Complex Matrices

Journal of the American Chemical Society, 131(20), 6944–6945.

Label-Free Colorimetric Screening of Nuclease Activity and Substrates by Using Unmodified Gold Nanoparticles

ChemBioChem, 10(12), 1973–1977.

Micromagnetic selection of aptamers in microfluidic channels

Proceedings of the National Academy of Sciences, 106(9), 2989–2994.

On the Signaling of Electrochemical Aptamer-Based Sensors: Collision- and Folding-Based Mechanisms

Electroanalysis, 21(11), 1267–1271.

i-Motif Quadruplex DNA-Based Biosensor for Distinguishing Single- and Multiwalled Carbon Nanotubes

Journal of the American Chemical Society, 131(38), 13813–13818.

Optimization of Electrochemical Aptamer-Based Sensors via Optimization of Probe Packing Density and Surface Chemistry

Langmuir, 24(18), 10513–10518.

Optimization of a Reusable, DNA Pseudoknot-Based Electrochemical Sensor for Sequence-Specific DNA Detection in Blood Serum

Analytical Chemistry, 81(2), 656–661.

Catalytic polynucleotide and its use for determination of analytes

(US Patent No. 20070254282).

Electrochemical Detection of Parts-Per-Billion Lead via an Electrode-Bound DNAzyme Assembly

Journal of the American Chemical Society, 129(2), 262–263.

Label-Free Electrochemical Detection of DNA in Blood Serum via Target-Induced Resolution of an Electrode-Bound DNA Pseudoknot

Journal of the American Chemical Society, 129(39), 11896–11897.

Preparation of electrode-immobilized, redox-modified oligonucleotides for electrochemical DNA and aptamer-based sensing

Nature Protocols, 2(11), 2875–2880.

Catalytic Beacons for the Detection of DNA and Telomerase Activity

In D. Morrison, F. Milanovich, D. Ivnitski, & T. R. Austin (Eds.), Defense against Bioterror (pp. 199–205).

Novel sensors with nanoparticle probe

(Patent Application No. WO2006008742).

Single-step electronic detection of femtomolar DNA by target-induced strand displacement in an electrode-bound duplex

Proceedings of the National Academy of Sciences, 103(45), 16677–16680.

A Reagentless Signal-On Architecture for Electronic, Aptamer-Based Sensors via Target-Induced Strand Displacement

Journal of the American Chemical Society, 127(51), 17990–17991.

An OsII-Bisbipyridine-4-Picolinic Acid Complex Mediates the Biocatalytic Growth of Au Nanoparticles: Optical Detection of Glucose and Acetylcholine Esterase Inhibition

Chemistry - A European Journal, 11(9), 2698–2704.

Inhibition of the Acetycholine Esterase-Stimulated Growth of Au Nanoparticles:  Nanotechnology-Based Sensing of Nerve Gases

Nano Letters, 5(4), 649–653.

Label-Free Electronic Detection of Thrombin in Blood Serum by Using an Aptamer-Based Sensor

Angewandte Chemie International Edition, 44(34), 5456–5459.

Optical and Electrochemical Detection of NADH and of NAD+-Dependent Biocatalyzed Processes by the Catalytic Deposition of Copper on Gold Nanoparticles

Small, 1(2), 213–216.

Shape and color of Au nanoparticles follow biocatalytic processes

Langmuir, 21(13), 5659–5662.

Amplified Chemiluminescence Surface Detection of DNA and Telomerase Activity Using Catalytic Nucleic Acid Labels

Analytical Chemistry, 76(7), 2152–2156.

Aptamer-Functionalized Au Nanoparticles for the Amplified Optical Detection of Thrombin

Journal of the American Chemical Society, 126(38), 11768–11769.

Catalytic Beacons for the Detection of DNA and Telomerase Activity

Journal of the American Chemical Society, 126(24), 7430–7431.

Catalytic Growth of Au Nanoparticles by NAD(P)H Cofactors: Optical Sensors for NAD(P)+-Dependent Biocatalyzed Transformations

Angewandte Chemie International Edition, 43(34), 4519–4522.

DNAzyme-Functionalized Au Nanoparticles for the Amplified Detection of DNA or Telomerase Activity

Nano Letters, 4(9), 1683–1687.

Electrical contacting of glucose oxidase by DNA-templated polyaniline wires on surfaces

Electrochemistry Communications, 6(10), 1057–1060.

Lighting Up Biochemiluminescence by the Surface Self-Assembly of DNA-Hemin Complexes

ChemBioChem, 5(3), 374–379.

"Plugging into Enzymes": Nanowiring of Redox Enzymes by a Gold Nanoparticle

Science, 299(5614), 1877–1881.