@misc{yuan_shen_salmon_2023, title={Developing Enzyme Immobilization with Fibrous Membranes: Longevity and Characterization Considerations}, volume={13}, ISSN={["2077-0375"]}, url={https://doi.org/10.3390/membranes13050532}, DOI={10.3390/membranes13050532}, abstractNote={Fibrous membranes offer broad opportunities to deploy immobilized enzymes in new reactor and application designs, including multiphase continuous flow-through reactions. Enzyme immobilization is a technology strategy that simplifies the separation of otherwise soluble catalytic proteins from liquid reaction media and imparts stabilization and performance enhancement. Flexible immobilization matrices made from fibers have versatile physical attributes, such as high surface area, light weight, and controllable porosity, which give them membrane-like characteristics, while simultaneously providing good mechanical properties for creating functional filters, sensors, scaffolds, and other interface-active biocatalytic materials. This review examines immobilization strategies for enzymes on fibrous membrane-like polymeric supports involving all three fundamental mechanisms of post-immobilization, incorporation, and coating. Post-immobilization offers an infinite selection of matrix materials, but may encounter loading and durability issues, while incorporation offers longevity but has more limited material options and may present mass transfer obstacles. Coating techniques on fibrous materials at different geometric scales are a growing trend in making membranes that integrate biocatalytic functionality with versatile physical supports. Biocatalytic performance parameters and characterization techniques for immobilized enzymes are described, including several emerging techniques of special relevance for fibrous immobilized enzymes. Diverse application examples from the literature, focusing on fibrous matrices, are summarized, and biocatalyst longevity is emphasized as a critical performance parameter that needs increased attention to advance concepts from lab scale to broader utilization. This consolidation of fabrication, performance measurement, and characterization techniques, with guiding examples highlighted, is intended to inspire future innovations in enzyme immobilization with fibrous membranes and expand their uses in novel reactors and processes.}, number={5}, journal={MEMBRANES}, author={Yuan, Yue and Shen, Jialong and Salmon, Sonja}, year={2023}, month={May} }
 @article{shen_yuan_salmon_2022, title={Durable and Versatile Immobilized Carbonic Anhydrase on Textile Structured Packing for CO2 Capture}, volume={12}, ISSN={["2073-4344"]}, url={https://www.mdpi.com/2073-4344/12/10/1108}, DOI={10.3390/catal12101108}, abstractNote={High-performance carbon dioxide (CO2)-capture technologies with low environmental impact are necessary to combat the current climate change crisis. Durable and versatile “drop-in-ready” textile structured packings with covalently immobilized carbonic anhydrase (CA) were created as efficient, easy to handle catalysts for CO2 absorption in benign solvents. The hydrophilic textile structure itself contributed high surface area and superior liquid transport properties to promote gas-liquid reactions that were further enhanced by the presence of CA, leading to excellent CO2 absorption efficiencies in lab-scale tests. Mechanistic investigations revealed that CO2 capture efficiency depended primarily on immobilized enzymes at or near the surface, whereas polymer entrapped enzymes were more protected from external stressors than those exposed at the surface, providing strategies to optimize performance and durability. Textile packing with covalently attached enzyme aggregates retained 100% of the initial 66.7% CO2 capture efficiency over 71-day longevity testing and retained 85% of the initial capture efficiency after 1-year of ambient dry storage. Subsequent stable performance in a 500 h continuous liquid flow scrubber test emphasized the material robustness. Biocatalytic textile packings performed well with different desirable solvents and across wide CO2 concentration ranges that are critical for CO2 capture from coal and natural gas-fired power plants, from natural gas and biogas for fuel upgrading, and directly from air.}, number={10}, journal={CATALYSTS}, author={Shen, Jialong and Yuan, Yue and Salmon, Sonja}, year={2022}, month={Oct} }
 @article{yuan_zhang_bilheux_salmon_2021, title={Biocatalytic Yarn for Peroxide Decomposition with Controlled Liquid Transport}, volume={8}, ISSN={["2196-7350"]}, url={https://publons.com/wos-op/publon/41826322/}, DOI={10.1002/admi.202002104}, abstractNote={AbstractA robust biocatalytic yarn with controllable liquid transport properties is created by coating thin layers of chitosan containing catalase onto a cellulosic yarn. The resulting material integrates enzyme catalytic functionality with protective coating properties of chitosan and structural functionality of the textile. Mild immobilization conditions and good affinity between the two polysaccharides minimize enzyme inactivation during the preparation steps and prevent enzyme from leaching during peroxide decomposition testing and washing, providing a novel and versatile enzyme immobilization strategy. The catalytic efficiency of enzymes in a reaction containing solid, liquid, and gas phases is facilitated when dissolved enzyme substrate is transported by liquid flowing through the coated textile structure. The flow‐through configuration decomposes at least two times more peroxide in a twenty‐times smaller reaction zone volume compared to a stirred tank configuration. Liquid transport through the yarn and liquid spatial distribution within the yarn are investigated by in situ neutron radiography and neutron computed tomography, revealing a constrained wicking mechanism that benefits biocatalytic yarn performance. This new class of sustainable and flexible biocatalytic textile matrices has beneficial multifunctional properties, not previously described, that are applicable for numerous small‐ and large‐scale applications including controlled flow reactors and reactive filtration.}, number={7}, journal={ADVANCED MATERIALS INTERFACES}, publisher={Wiley}, author={Yuan, Yue and Zhang, Yuxuan and Bilheux, Hassina and Salmon, Sonja}, year={2021}, month={Apr} }
 @article{yuan_li_leite_zhang_bonnesen_labbe_weiss_pingali_hong_urban_et al._2021, title={Biosynthesis and characterization of deuterated chitosan in filamentous fungus and yeast}, volume={257}, ISSN={["1879-1344"]}, url={https://publons.com/wos-op/publon/34591276/}, DOI={10.1016/j.carbpol.2021.117637}, abstractNote={Deuterated chitosan was produced from the filamentous fungus Rhizopus oryzae, cultivated with deuterated glucose in H2O medium, without the need for conventional chemical deacetylation. After extraction and purification, the chemical composition and structure were determined by Fourier-transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), and small-angle neutron scattering (SANS). 13C NMR experiments provided additional information about the position of the deuterons in the glucoseamine backbone. The NMR spectra indicated that the deuterium incorporation at the non-exchangeable hydrogen positions of the aminoglucopyranosyl ring in the C3 – C5 positions was at least 60–80 %. However, the C2 position was deuterated at a much lower level (6%). Also, SANS showed that the structure of deuterated chitosan was very similar compared to the non-deuterated counterpart. The most abundant radii of the protiated and deuterated chitosan fibers were 54 Å and 60 Å, respectively, but there is a broader distribution of fiber radii in the protiated chitosan sample. The highly deuterated, soluble fungal chitosan described here can be used as a model material for studying chitosan-enzyme complexes for future neutron scattering studies. Because the physical behavior of non-deuterated fungal chitosan mimicked that of shrimp shell chitosan, the methods presented here represent a new approach to producing a high quality deuterated non-animal-derived aminopolysaccharide for studying the structure-function association of biocomposite materials in drug delivery, tissue engineering and other bioactive chitosan-based composites.}, journal={CARBOHYDRATE POLYMERS}, author={Yuan, Yue and Li, Hui and Leite, Wellington and Zhang, Qiu and Bonnesen, Peter V and Labbe, Jessy L. and Weiss, Kevin L. and Pingali, Sai Venkatesh and Hong, Kunlun and Urban, Volker S. and et al.}, year={2021}, month={Apr} }
 @article{park_yuan_choi_choi_kim_2018, title={Doxorubicin release controlled by induced phase separation and use of a co-solvent}, volume={11}, number={5}, journal={Materials}, author={Park, S. C. and Yuan, Y. and Choi, K. and Choi, S. O. and Kim, J.}, year={2018} }
 @article{yuan_choi_choi_kim_2018, title={Early stage release control of an anticancer drug by drug-polymer miscibility in a hydrophobic fiber-based drug delivery system}, volume={8}, ISSN={["2046-2069"]}, DOI={10.1039/c8ra01467a}, abstractNote={The drug release profiles of doxorubicin-loaded electrospun fiber mats were investigated with regard to drug-polymer miscibility, fiber wettability and degradability.}, number={35}, journal={RSC ADVANCES}, author={Yuan, Yue and Choi, Kyoungju and Choi, Seong-O and Kim, Jooyoun}, year={2018}, pages={19791–19803} }