Works (35)

Updated: August 2nd, 2023 10:20

2021 journal article

Evaluation of UBE3A antibodies in mice and human cerebral organoids

SCIENTIFIC REPORTS, 11(1).

By: D. Sen n, Z. Drobna n & A. Keung n

MeSH headings : Angelman Syndrome / genetics; Angelman Syndrome / pathology; Animals; Antibodies / genetics; Antibodies / immunology; Autistic Disorder / genetics; Autistic Disorder / pathology; Cerebellum / cytology; Disease Models, Animal; Genomic Imprinting; Humans; Mice; Neural Stem Cells / metabolism; Neurons / metabolism; Neurons / pathology; Organoids / cytology; Pluripotent Stem Cells; Ubiquitin-Protein Ligases / genetics; Ubiquitin-Protein Ligases / immunology
TL;DR: While four antibodies show specific localization patterns in both mouse brain sections and human cerebral organoids, these antibodies varied significantly in background signals and staining patterns in undifferentiated human pluripotent stem cells. (via Semantic Scholar)
Source: Web Of Science
Added: July 19, 2021

2020 journal article

Activation of Lrrk2 and alpha-Synuclein in substantia nigra, striatum, and cerebellum after chronic exposure to arsenite

TOXICOLOGY AND APPLIED PHARMACOLOGY, 408.

By: Z. Drobna n

author keywords: Lrrk2; alpha-Synuclein; Arsenite; Striatum; Substantia nigra; Cerebellum
MeSH headings : Animals; Arsenic Poisoning / genetics; Arsenic Poisoning / metabolism; Arsenic Poisoning / physiopathology; Arsenites / toxicity; Cerebellum / drug effects; Cerebellum / metabolism; Corpus Striatum / drug effects; Leucine-Rich Repeat Serine-Threonine Protein Kinase-2 / metabolism; Male; Mice, Inbred C57BL; Muscle Strength / drug effects; Phosphorylation / drug effects; Substantia Nigra / drug effects; Substantia Nigra / metabolism; Transcriptome / drug effects; alpha-Synuclein / metabolism
TL;DR: Chronic arsenite exposure altered transcripts of glutathione redox reactions and serotonin receptor signaling in striatum, axonal guidance signaling, NF-κB and androgen signaling in substantia nigra and mitochondrial dysfunction, oxidative phosphorylation, apoptosis and sirtuin signaling in the cerebellum. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: December 11, 2020

2020 journal article

Human Cerebral Organoids Reveal Early Spatiotemporal Dynamics and Pharmacological Responses of UBE3A

STEM CELL REPORTS, 15(4), 845–854.

By: D. Sen n, A. Voulgaropoulos n, Z. Drobna n & A. Keung n

MeSH headings : Angelman Syndrome / pathology; Cell Line; Cell Nucleus / drug effects; Cell Nucleus / metabolism; Cerebrum / metabolism; Genomic Imprinting / drug effects; Humans; Neurons / drug effects; Neurons / metabolism; Organoids / drug effects; Organoids / metabolism; Time Factors; Topoisomerase Inhibitors / pharmacology; Ubiquitin-Protein Ligases / metabolism
TL;DR: Human cerebral organoids are established as an important model for studying UBE3A and motivates their broader use in understanding complex neurodevelopmental disorders. (via Semantic Scholar)
UN Sustainable Development Goal Categories
4. Quality Education (OpenAlex)
Source: Web Of Science
Added: November 9, 2020

2019 journal article

Bisphenol F has different effects on preadipocytes differentiation and weight gain in adult mice as compared with Bisphenol A and S

TOXICOLOGY, 420, 66–72.

By: Z. Drobna n, A. Talarovicova n, H. Schrader n, T. Fennell n, R. Snyder n & E. Rissman n

author keywords: Bisphenol; Metabolism; Fat; BPF; Alternatives to BPA; Diabetes
MeSH headings : 3T3-L1 Cells; Adipocytes / drug effects; Adipocytes / metabolism; Adipogenesis / drug effects; Adipogenesis / genetics; Animals; Benzhydryl Compounds / toxicity; Dose-Response Relationship, Drug; Endocrine Disruptors / toxicity; Gene Expression Regulation; Male; Mice; Mice, Inbred ICR; Phenols / toxicity; Sulfones / toxicity; Time Factors; Weight Gain / drug effects
TL;DR: The results suggest that BPS has a strong potential to be obesogenic while effects of BPF are subtler and potentially in the opposite direction. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: June 17, 2019

2019 journal article

Transgenerational Bisphenol A Causes Deficits in Social Recognition and Alters Postsynaptic Density Genes in Mice

ENDOCRINOLOGY, 160(8), 1854–1867.

By: J. Wolstenholme*, Z. Drobna n, A. Henriksen*, J. Goldsby*, R. Stevenson*, J. Irvin n, J. Flaws*, E. Rissman n

MeSH headings : Animals; Benzhydryl Compounds / toxicity; Brain / drug effects; Brain / metabolism; Female; Fetus / drug effects; Male; Mice; Mice, Inbred C57BL; Nerve Tissue Proteins / physiology; Phenols / toxicity; Post-Synaptic Density / drug effects; Social Behavior
TL;DR: Transgenerational BPA exposure disrupted social interactions in mice and dysregulated normal expression of PSD genes during neural development, suggesting that BPA may contribute, in a transgenerational manner, to neurodevelopmental diseases. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 19, 2019

2018 journal article

Transgenerational Effects of Bisphenol A on Gene Expression and DNA Methylation of Imprinted Genes in Brain

ENDOCRINOLOGY, 159(1), 132–144.

By: Z. Drobna n, A. Henriksen*, J. Wolstenholme*, C. Montiel n, P. Lambeth*, S. Shang*, E. Harris*, C. Zhou* ...

MeSH headings : Animals; Benzhydryl Compounds / toxicity; Brain / drug effects; Brain / metabolism; Crosses, Genetic; DNA Methylation / drug effects; Endocrine Disruptors / toxicity; Epigenesis, Genetic / drug effects; Female; Fetal Development / drug effects; Gene Expression Regulation, Developmental / drug effects; Hypothalamus, Anterior / drug effects; Hypothalamus, Anterior / metabolism; Lactation; Male; Maternal Exposure / adverse effects; Mice, Inbred Strains; Neurons / drug effects; Neurons / metabolism; Phenols / toxicity; Pregnancy; Preoptic Area / drug effects; Preoptic Area / metabolism; RNA, Long Noncoding / agonists; RNA, Long Noncoding / genetics; RNA, Long Noncoding / metabolism; Random Allocation; Septal Nuclei / drug effects; Septal Nuclei / metabolism; Sex Characteristics
TL;DR: This gene, maternally expressed gene 3 (Meg3), has been associated with EDCs and neurobehavioral phenotypes and may be a biomarker indicative of early life environmental perturbation. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2017 journal article

Metabolomic profiles of arsenic (+3 oxidation state) methyltransferase knockout mice: effect of sex and arsenic exposure

Archives of Toxicology, 91(1), 189–202.

By: M. Huang*, C. Douillet*, M. Su*, K. Zhou*, T. Wu*, W. Chen*, J. Galanko*, Z. Drobná* ...

author keywords: Arsenic; Metabolomics; As3mt knockout; Mice; Urine; Plasma
MeSH headings : Amino Acids / metabolism; Animals; Arsenic / blood; Arsenic / metabolism; Arsenic / toxicity; Arsenic / urine; Arsenic Poisoning / blood; Arsenic Poisoning / enzymology; Arsenic Poisoning / metabolism; Arsenic Poisoning / urine; Arsenicals / blood; Arsenicals / metabolism; Arsenicals / urine; Biomarkers / blood; Biomarkers / urine; Biotransformation; Carbohydrate Metabolism / drug effects; Drug Resistance; Energy Metabolism / drug effects; Female; Lipid Metabolism / drug effects; Male; Metabolome / drug effects; Methylation; Methyltransferases / genetics; Methyltransferases / metabolism; Mice, Inbred C57BL; Mice, Knockout; Sex Characteristics; Toxicokinetics; Water Pollutants, Chemical / blood; Water Pollutants, Chemical / metabolism; Water Pollutants, Chemical / toxicity; Water Pollutants, Chemical / urine
TL;DR: It is suggested that As3mt KO alters major metabolic pathways in a sex-specific manner, independent of iAs treatment, and that As 3mt may be involved in other cellular processes beyond iAs methylation. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Source: Crossref
Added: December 21, 2020

2017 journal article

Neonatal Metabolomic Profiles Related to Prenatal Arsenic Exposure

ENVIRONMENTAL SCIENCE & TECHNOLOGY, 51(1), 625–633.

By: J. Laine, K. Bailey, A. Olshan, L. Smeester, Z. Drobna n, M. Styblo, C. Douillet, G. Garcia-Vargas* ...

MeSH headings : Arsenic; Arsenicals; Environmental Exposure; Female; Humans; Infant, Newborn; Metabolomics; Mexico; Pregnancy
TL;DR: NMR spectroscopy-based metabolomic analysis was used to identify metabolites in neonate cord serum associated with prenatal iAs exposure in participants from the Biomarkers of Exposure to ARsenic (BEAR) pregnancy cohort, in Gómez Palacio, Mexico, demonstrating the potential utility of metabolites as biomarkers/indicators of in utero environmental exposure. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2017 journal article

The Association of Arsenic Exposure and Metabolism With Type 1 and Type 2 Diabetes in Youth: The SEARCH Case-Control Study

DIABETES CARE, 40(1), 46–53.

By: M. Grau-Perez*, C. Kuo*, M. Spratlen*, K. Thayer*, M. Mendez*, R. Hamman*, D. Dabelea*, J. Adgate* ...

MeSH headings : Adolescent; Arsenic / blood; Biomarkers / blood; Case-Control Studies; Diabetes Mellitus, Type 1 / blood; Diabetes Mellitus, Type 2 / blood; Environmental Exposure / analysis; Female; Folic Acid / blood; Humans; Male; Vitamin B 12 / blood; Young Adult
TL;DR: The association of arsenic with type 1 and type 2 diabetes in youth in the SEARCH for Diabetes in Youth Case-Control study and the potential interaction of folate and vitamin B12 with arsenic metabolism on the odds of diabetes support further research on the role of arsenic metabolism in type 1 diabetes. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2016 journal article

Analysis of maternal polymorphisms in arsenic (+3 oxidation state)-methyltransferase AS3MT and fetal sex in relation to arsenic metabolism and infant birth outcomes: Implications for risk analysis

REPRODUCTIVE TOXICOLOGY, 61, 28–38.

By: Z. Drobna n, E. Martin*, K. Kim*, L. Smeester*, P. Bommarito*, M. Rubio-Andrade*, G. Garcia-Vargas*, M. Styblo*, F. Zou*, R. Fry*

author keywords: AS3MT; Arsenic; Genotype; Prenatal exposure; Birth outcomes
MeSH headings : Adolescent; Adult; Arsenic / metabolism; Arsenic / urine; Female; Genotype; Humans; Infant, Newborn; Male; Methyltransferases / genetics; Polymorphism, Single Nucleotide; Pregnancy; Pregnancy Outcome; Risk Assessment; Sex Factors; Young Adult
TL;DR: Assessment of relationships between single nucleotide polymorphisms in AS3MT and urinary concentrations of iAs and its methylated metabolites in pregnant women and infants highlighted a potential sex-dependence of the relationships among maternal genotype, iAs metabolism and infant health outcomes. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2016 journal article

Arsenic exposure and cardiometabolic risk in Chihuahua, Mexico

Toxicology Letters, 259, S32–S33.

By: M. Mendez*, C. González-Horta*, B. Sánchez-Ramírez*, L. Ballinas-Casarrubias*, R. Cerón, D. Morales, F. Terrazas, C. María* ...

Source: Crossref
Added: December 21, 2020

2016 journal article

Association Between Variants in Arsenic (+3 Oxidation State) Methyltranserase (AS3MT) and Urinary Metabolites of Inorganic Arsenic: Role of Exposure Level

TOXICOLOGICAL SCIENCES, 153(1), 112–123.

By: X. Xu, Z. Drobna n, V. Voruganti, K. Barron, C. Gonzalez-Horta*, B. Sanchez-Ramirez*, L. Ballinas-Casarrubias*, R. Hernandez Ceron ...

author keywords: Arsenic (+3 oxidation state) methyltranserase polymorphism; arsenic; drinking water; urinary arsenic; arsenic metabolites; methylation capacity
MeSH headings : Adult; Arsenic / analysis; Arsenic / toxicity; Arsenic / urine; Cross-Sectional Studies; Drinking Water / chemistry; Environmental Exposure; Female; Genotype; Humans; Limit of Detection; Male; Methyltransferases / genetics; Methyltransferases / metabolism; Polymorphism, Single Nucleotide
TL;DR: It is suggested that iAs exposure may influence the extent to which several AS3MT variants affect iAs metabolism, and the variants most strongly associated withiAs metabolism-and perhaps with susceptibility to iAs-associated disease-may vary in settings with exposure level. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2016 journal article

Oxidation state specific analysis of arsenic species in tissues of wild-type and arsenic (+ 3 oxidation state) methyltransferase-knockout mice

Journal of Environmental Sciences, 49, 104–112.

By: J. Currier*, C. Douillet*, Z. Drobná* & M. Stýblo*

author keywords: Arsenic speciation analysis; Hydride generation-cryotrapping-atomic absorption spectrometry; Arsenic (+3 oxidation state) methyltransferase; As3mt knockout mice
MeSH headings : Animals; Arsenic / toxicity; Arsenicals / pharmacology; Male; Methyltransferases; Mice; Mice, Inbred C57BL; Oxidation-Reduction; Water Pollutants, Chemical / toxicity
TL;DR: This study is the first to determine oxidation states of As species in tissues of As3mt-KO mice, suggesting that intestinal bacteria are not a major source of methylated As. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Source: Crossref
Added: December 21, 2020

2015 journal article

Identification of Novel Gene Targets and Putative Regulators of Arsenic-Associated DNA Methylation in Human Urothelial Cells and Bladder Cancer

Chemical Research in Toxicology, 28(6), 1144–1155.

By: J. Rager*, S. Tilley*, S. Tulenko*, L. Smeester*, P. Ray*, A. Yosim*, J. Currier*, M. Ishida* ...

MeSH headings : Adult; Aged; Arsenic / metabolism; Arsenic / toxicity; Cell Transformation, Neoplastic / chemically induced; DNA Methylation / drug effects; DNA Methylation / genetics; Female; Humans; Middle Aged; Urinary Bladder Neoplasms / chemically induced; Urinary Bladder Neoplasms / genetics; Urinary Bladder Neoplasms / pathology; Urothelium / cytology; Urothelium / drug effects; Young Adult
TL;DR: Both the arsenic- and cancer-associated genes are enriched for the binding sites of common transcription factors known to play roles in carcinogenesis, demonstrating a novel potential mechanistic link between iAs exposure and bladder cancer. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Crossref
Added: February 5, 2020

2013 journal article

Arsenic and the Epigenome: Interindividual Differences in Arsenic Metabolism Related to Distinct Patterns of DNA Methylation

Journal of Biochemical and Molecular Toxicology, 27(2), 106–115.

By: K. Bailey*, M. Wu*, W. Ward*, L. Smeester*, J. Rager*, G. García-Vargas, L. Del Razo*, Z. Drobná*, M. Stýblo*, R. Fry*

MeSH headings : Arsenic / pharmacokinetics; Arsenic / toxicity; Arsenic Poisoning / genetics; Arsenic Poisoning / metabolism; Arsenic Poisoning / pathology; Biomarkers / metabolism; DNA Methylation / drug effects; Diabetes Mellitus / chemically induced; Diabetes Mellitus / genetics; Diabetes Mellitus / metabolism; Diabetes Mellitus / pathology; Epigenesis, Genetic / drug effects; Epigenomics / methods; Female; Genome-Wide Association Study; Humans; Leukocytes / metabolism; Leukocytes / pathology; Male
TL;DR: Methylation patterns of genes with known associations with DM were associated with urinary concentrations of specific iAs metabolites, and future studies will determine whether these DNA methylation profiles provide mechanistic insight into the development of iAs‐associated disease, predict disease risk, and/or serve as biomarkers of inorganic arsenic exposure in humans. (via Semantic Scholar)
Source: Crossref
Added: December 21, 2020

2013 journal article

Prenatal arsenic exposure and the epigenome: Altered microRNAs associated with innate and adaptive immune signaling in newborn cord blood

Environmental and Molecular Mutagenesis, 55(3), 196–208.

By: J. Rager*, K. Bailey*, L. Smeester*, S. Miller*, J. Parker*, J. Laine*, Z. Drobná*, J. Currier* ...

author keywords: gene expression; arsenic; prenatal; epigenetics; microRNAs
MeSH headings : Adaptive Immunity / physiology; Adult; Arsenic / toxicity; Arsenic / urine; Biomarkers / metabolism; Cohort Studies; Drinking Water / chemistry; Epigenesis, Genetic; Epigenomics; Female; Fetal Blood / drug effects; Fetal Blood / metabolism; Gene Expression Profiling; Genome-Wide Association Study; Humans; Infant, Newborn; Maternal Exposure; MicroRNAs / metabolism; Pregnancy; RNA, Messenger / metabolism; Signal Transduction; Transcription, Genetic; Water Pollutants, Chemical / toxicity
TL;DR: Results of this study highlight miRNAs as novel responders to prenatal arsenic exposure that may contribute to associated immune response perturbations. (via Semantic Scholar)
Source: Crossref
Added: December 21, 2020

2012 journal article

Environmental exposure to arsenic, AS3MT polymorphism and prevalence of diabetes in Mexico

Journal of Exposure Science & Environmental Epidemiology, 23(2), 151–155.

By: Z. Drobná*, L. Del Razo*, G. García-Vargas*, L. Sánchez-Peña*, A. Barrera-Hernández*, M. Stýblo*, D. Loomis*

author keywords: biomonitoring; disease; emerging contaminants; metals
MeSH headings : Adolescent; Adult; Arsenic / metabolism; Arsenic / toxicity; Diabetes Mellitus / epidemiology; Environmental Exposure; Female; Humans; Male; Methyltransferases / genetics; Mexico / epidemiology; Middle Aged; Polymorphism, Single Nucleotide; Prevalence; Young Adult
TL;DR: Carriers of 287T and 4965C may produce more DMAsIII and be more likely to develop diabetes when exposed to arsenic, and individuals with M287T and G4965C polymorphisms had higher levels of urinary DMAs III and were more frequently diabetic than the respective wild-type carriers. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Source: Crossref
Added: January 21, 2021

2012 journal article

Methylation of arsenic by recombinant human wild-type arsenic (+3 oxidation state) methyltransferase and its methionine 287 threonine (M287T) polymorph: Role of glutathione

Toxicology and Applied Pharmacology, 264(1), 121–130.

By: L. Ding*, R. Saunders*, Z. Drobná*, F. Walton*, P. Xun*, D. Thomas*, M. Stýblo*

author keywords: Arsenic methylation; AS3MT polymorphism; Thioredoxin reductase; Glutathione
MeSH headings : Animals; Arsenicals / metabolism; Arsenites / metabolism; Glutathione / metabolism; Humans; Methylation / drug effects; Methyltransferases / genetics; Methyltransferases / metabolism; Phosphines / metabolism; Polymorphism, Genetic; Rats; S-Adenosylmethionine / metabolism; Thioredoxin-Disulfide Reductase / metabolism
TL;DR: 1mM GSH modulates AS3MT activity, increasing both methylation rates and yield of DMAs(III) and, ultimately, to differences in the disease susceptibility in individuals chronically exposed to inorganic arsenic. (via Semantic Scholar)
Source: Crossref
Added: January 21, 2021

2012 journal article

The epigenetic effects of a high prenatal folate intake in male mouse fetuses exposed in utero to arsenic

Toxicology and Applied Pharmacology, 264(3), 439–450.

By: V. Tsang*, R. Fry*, M. Niculescu*, J. Rager*, J. Saunders*, D. Paul*, S. Zeisel*, M. Waalkes*, M. Stýblo*, Z. Drobná*

author keywords: Arsenic; Folate; Epigenetics; DNA methylation; Transplacental exposure; CD1 mice
MeSH headings : Animals; Arsenites / administration & dosage; Arsenites / toxicity; Epigenomics; Female; Fetal Weight / drug effects; Fetus / drug effects; Folic Acid / administration & dosage; Folic Acid / blood; Folic Acid / pharmacology; Gene Expression Regulation, Developmental / drug effects; Liver / drug effects; Liver / embryology; Liver / metabolism; Male; Mice; Pregnancy; S-Adenosylhomocysteine / metabolism; S-Adenosylmethionine / metabolism; Sodium Compounds / administration & dosage; Sodium Compounds / toxicity
TL;DR: A combined in utero exposure to iAs and a high folate intake may adversely influence DNA methylation profiles and weight of fetuses, compromising fetal development and possibly increasing the risk for early-onset of disease in offspring. (via Semantic Scholar)
Source: Crossref
Added: January 21, 2021

2011 journal article

Epigenetic Changes in Individuals with Arsenicosis

Chemical Research in Toxicology, 24(2), 165–167.

By: L. Smeester*, J. Rager, K. Bailey, X. Guan*, N. Smith, G. García-Vargas, L. Del Razo*, Z. Drobná* ...

MeSH headings : Arsenic / toxicity; Arsenic Poisoning / genetics; CpG Islands; DNA Methylation; Environmental Exposure / adverse effects; Epigenesis, Genetic; Humans; Mexico; Water Pollutants, Chemical / toxicity; Water Supply
TL;DR: A large interactome of hypermethylated genes that are enriched for their involvement in arsenic-associated diseases, such as cancer, heart disease, and diabetes are identified, and an arsenic-induced tumor suppressorome is uncovered, a complex of 17 tumor suppressors known to be silenced in human cancers. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2011 journal article

Identification of theGST-T1andGST-M1Null Genotypes Using High Resolution Melting Analysis

Chemical Research in Toxicology, 25(1), 216–224.

By: Z. Drobná*, L. Del Razo*, G. Garcia-Vargas*, B. Sánchez-Ramírez*, C. González-Horta*, L. Ballinas-Casarrubias*, D. Loomis*, M. Stýblo*

MeSH headings : Adult; Aged; DNA / genetics; Female; Genotype; Glutathione Transferase / genetics; Hepatocytes / enzymology; Humans; Male; Mexico; Middle Aged; Multiplex Polymerase Chain Reaction / methods; Polymorphism, Genetic; beta-Globins / genetics
TL;DR: A multiplex High Resolution Melting PCR (HRM-PCR) analysis using a LightCycler480 instrument is developed that evaluated GST-T1 and GST-M1 genotypes in 504 DNA samples isolated from the blood of individuals residing in Zimapan, Lagunera, and Chihuahua regions in Mexico. (via Semantic Scholar)
UN Sustainable Development Goal Categories
10. Reduced Inequalities (OpenAlex)
Source: Crossref
Added: January 21, 2021

2010 journal article

Interspecies differences in metabolism of arsenic by cultured primary hepatocytes

Toxicology and Applied Pharmacology, 245(1), 47–56.

By: Z. Drobná*, F. Walton*, A. Harmon*, D. Thomas* & M. Stýblo*

author keywords: Arsenic; Primary hepatocytes; Metabolism; As3mt; Mammalian species
MeSH headings : Animals; Arsenic / metabolism; Arsenic / toxicity; Cells, Cultured; Dogs; Female; Glutathione / metabolism; Hepatocytes / drug effects; Hepatocytes / enzymology; Hepatocytes / metabolism; Humans; Macaca mulatta; Male; Methylation; Methyltransferases / metabolism; Mice; Middle Aged; Rabbits; Rats; Species Specificity
TL;DR: Immunoblot analyses indicate that the superior arsenic methylation capacities of dog, rat and monkey hepatocytes examined in this study may be associated with a higher As3mt expression, which may also contribute to the interspecies differences in the hepatocyte capacity to methylate iAs. (via Semantic Scholar)
Source: Crossref
Added: January 21, 2021

2009 journal article

Association of AS3MT polymorphisms and the risk of premalignant arsenic skin lesions

Toxicology and Applied Pharmacology, 239(2), 200–207.

By: O. Valenzuela*, Z. Drobná*, E. Hernández-Castellanos*, L. Sánchez-Peña*, G. García-Vargas*, V. Borja-Aburto*, M. Stýblo*, L. Del Razo*

author keywords: Arsenic-skin lesion; AS3MT polymorphism; Arsenic metabolism
MeSH headings : Adolescent; Adult; Arsenic / toxicity; Arsenic / urine; Case-Control Studies; Cross-Sectional Studies; DNA / genetics; Environmental Exposure / adverse effects; Environmental Exposure / analysis; Female; Gene Frequency; Genotype; Humans; Male; Methyltransferases / genetics; Mexico / epidemiology; Middle Aged; Mouth Mucosa / cytology; Polymorphism, Single Nucleotide; Precancerous Conditions / chemically induced; Precancerous Conditions / enzymology; Precancerous Conditions / epidemiology; Precancerous Conditions / genetics; Skin Neoplasms / chemically induced; Skin Neoplasms / enzymology; Skin Neoplasms / epidemiology; Skin Neoplasms / genetics; Water Pollutants, Chemical / toxicity; Water Pollutants, Chemical / urine; Young Adult
TL;DR: Findings indicate that Met287Thr influences the susceptibility to premalignant As skin lesions and might be at increased risk for other adverse health effects of iAs exposure. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Source: Crossref
Added: January 21, 2021

2009 journal article

Disruption of the Arsenic (+3 Oxidation State) Methyltransferase Gene in the Mouse Alters the Phenotype for Methylation of Arsenic and Affects Distribution and Retention of Orally Administered Arsenate

Chemical Research in Toxicology, 22(10), 1713–1720.

By: Z. Drobna*, H. Naranmandura*, K. Kubachka*, B. Edwards*, K. Herbin-Davis*, M. Styblo*, X. Le*, J. Creed* ...

MeSH headings : Administration, Oral; Animals; Arsenates / administration & dosage; Arsenates / pharmacokinetics; Arsenates / urine; Arsenic Poisoning; Arsenicals / metabolism; Arsenicals / urine; Female; Genotype; Humans; Male; Methyltransferases / genetics; Methyltransferases / metabolism; Mice; Mice, Inbred C57BL; Mice, Knockout; Phenotype; Tissue Distribution
TL;DR: A central role for As3mt in the metabolism of inorganic arsenic is confirmed and phenotypes for arsenic retention and distribution are markedly affected by the null genotype for arsenic methylation, indicating a close linkage between the metabolism and retention of arsenicals. (via Semantic Scholar)
Source: Crossref
Added: January 21, 2021

2009 journal article

Metabolism of arsenic in human liver: the role of membrane transporters

Archives of Toxicology, 84(1), 3–16.

By: Z. Drobná*, F. Walton*, D. Paul*, W. Xing*, D. Thomas* & M. Stýblo*

author keywords: Arsenic; Human liver; Membrane transport
MeSH headings : Aged; Aged, 80 and over; Animals; Arsenic / metabolism; Cell Membrane / metabolism; Cells, Cultured; Female; Glucose Transporter Type 2 / metabolism; Humans; Liver / metabolism; Male; Membrane Transport Proteins / metabolism; Methylation; Middle Aged; Multidrug Resistance-Associated Protein 2; Multidrug Resistance-Associated Proteins / metabolism
TL;DR: The results show that the retention of iAs and methylarsenic metabolites (MAs) by hepatocytes exposed to sub-micromolar concentrations of arsenite correlates negatively with MRP2 expression, and suggest that MRP 2 plays an important role in the efflux of DMAs, thus, regulating kinetics of the methylation reactions and accumulation of i as and MAs by human hepatocytes. (via Semantic Scholar)
Source: Crossref
Added: January 21, 2021

2008 journal article

Oxidation state specific generation of arsines from methylated arsenicals based on l-cysteine treatment in buffered media for speciation analysis by hydride generation-automated cryotrapping-gas chromatography-atomic absorption spectrometry with the multiatomizer

Spectrochimica Acta Part B: Atomic Spectroscopy, 63(3), 396–406.

By: T. Matoušek*, A. Hernández-Zavala*, M. Svoboda*, L. Langrová*, B. Adair*, Z. Drobná*, D. Thomas*, M. Stýblo*, J. Dědina*

author keywords: speciation analysis; arsenic; hydride generation atomic absorption spectrometry; cryotrapping; multiatornizer
TL;DR: This method permits a high-throughput speciation analysis of metabolites of inorganic arsenic in relatively complex biological matrices such as cell culture systems without sample pretreatment, thus preserving the distribution of tri- and pentavalent species. (via Semantic Scholar)
Source: Crossref
Added: January 21, 2021

2008 journal article

Speciation analysis of arsenic in biological matrices by automated hydride generation-cryotrapping-atomic absorption spectrometry with multiple microflame quartz tube atomizer (multiatomizer)

J. Anal. At. Spectrom., 23(3), 342–351.

By: A. Hernández-Zavala*, T. Matoušek*, Z. Drobná*, D. Paul*, F. Walton*, B. Adair*, J. Dědina*, D. Thomas*, M. Stýblo*

TL;DR: The automated HG-CT-AAS system provides an effective and sensitive tool for analysis of all major human metabolites of iAs in complex biological matrices and the capacity to detect methylated As(III)- and As(V)-species was verified. (via Semantic Scholar)
Source: Crossref
Added: January 21, 2021

2006 journal article

shRNA Silencing of AS3MT Expression Minimizes Arsenic Methylation Capacity of HepG2 Cells

Chemical Research in Toxicology, 19(7), 894–898.

By: Z. Drobná*, W. Xing*, D. Thomas* & M. Stýblo*

MeSH headings : Arsenic / metabolism; Arsenic / toxicity; Base Sequence; Cell Line, Tumor; Hepatocytes / drug effects; Hepatocytes / metabolism; Humans; Methylation / drug effects; Methyltransferases / antagonists & inhibitors; Methyltransferases / genetics; Methyltransferases / metabolism; Molecular Sequence Data; RNA Interference; RNA, Small Interfering / metabolism; RNA, Small Interfering / pharmacology
TL;DR: Data suggest that AS3MT is the major enzyme in the oxidative methylation of inorganic arsenic (iAs) in mammalian species, although an As3MT-independent process may contribute to iAs methylation in human hepatic cells. (via Semantic Scholar)
Source: Crossref
Added: January 21, 2021

2005 journal article

Arsenic (+3 oxidation state) methyltransferase and the inorganic arsenic methylation phenotype

Toxicology and Applied Pharmacology, 204(2), 164–169.

By: J. Li*, S. Waters*, Z. Drobna*, V. Devesa*, M. Styblo* & D. Thomas*

author keywords: arsenic; inorganic arsenic; oxidation state
MeSH headings : Animals; Arsenic / metabolism; Base Sequence / genetics; Chromosomes, Human, Pair 8 / genetics; Cysteine / chemistry; Cysteine / genetics; Evolution, Molecular; Gene Deletion; Gene Expression Profiling / methods; Genotype; Humans; Methylation; Methyltransferases / genetics; Methyltransferases / metabolism; Mice; Models, Molecular; Molecular Sequence Data; Oxidation-Reduction; Pan troglodytes / genetics; Pan troglodytes / metabolism; Phenotype; Rats; Species Specificity; Time Factors
TL;DR: The null phenotype for inorganic arsenic methylation in the chimpanzee is likely due to the deletion in the gene for arsenic (+3 oxidation state) methyltransferase that yields an inactive truncated protein. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Source: Crossref
Added: August 28, 2020

2005 journal article

Commonalities in Metabolism of Arsenicals

Environmental Chemistry, 2(3), 161.

By: B. Adair, S. Waters, V. Devesa, Z. Drobna*, M. Styblo & D. Thomas

author keywords: arsenic; bioavailability; contaminant uptake; metabolism-affecting agents
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Source: Crossref
Added: August 28, 2020

2005 journal article

Metabolism and toxicity of arsenic in human urothelial cells expressing rat arsenic (+3 oxidation state)-methyltransferase

Toxicology and Applied Pharmacology, 207(2), 147–159.

By: Z. Drobna*, S. Waters*, V. Devesa*, A. Harmon*, D. Thomas* & M. Styblo*

author keywords: As; methyltransferase; AS3MT; metabolism; toxicity; urinary bladder; human
MeSH headings : Animals; Arsenic / metabolism; Arsenic / toxicity; Cell Line; Cell Survival / drug effects; Methylation; Methyltransferases / physiology; Rats; Urinary Bladder / drug effects; Urinary Bladder / metabolism
TL;DR: The resistance of UROtsa/F35 cells to moderate concentrations of MAs(III) was linked to its rapid conversion to DMAs and efflux of DMAs, which is a key factor contributing to the toxicity of acute iAs exposures in methylating cells. (via Semantic Scholar)
Source: Crossref
Added: August 28, 2020

2004 journal article

Comprehensive analysis of arsenic metabolites by pH-specific hydride generation atomic absorption spectrometry

J. Anal. At. Spectrom., 19(11), 1460–1467.

By: V. Devesa*, L. Maria Del Razo, B. Adair*, Z. Drobná*, S. Waters*, M. Hughes*, M. Stýblo*, D. Thomas*

UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Source: Crossref
Added: January 21, 2021

2004 journal article

Inhibition of insulin-dependent glucose uptake by trivalent arsenicals: possible mechanism of arsenic-induced diabetes

Toxicology and Applied Pharmacology, 198(3), 424–433.

By: F. Walton*, A. Harmon, D. Paul, Z. Drobna, Y. Patel & M. Styblo

author keywords: arsenic; diabetes; methylation; glucose uptake; insulin; GLUT4; PKB; Akt
MeSH headings : Animals; Arsenicals / adverse effects; Arsenicals / metabolism; Arsenicals / pharmacology; Cell Survival / drug effects; Cells, Cultured; Diabetes Mellitus, Type 2 / chemically induced; Glucose / metabolism; Insulin / pharmacology; Methylation; Mice
TL;DR: Results suggest that trivalent arsenicals inhibit insulin-stimulated glucose uptake by interfering with the PKB/Akt-dependent mobilization of GLUT4 transporters in adipocytes, which may be, in part, responsible for the development of type-2 diabetes in individuals chronically exposed to iAs. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Source: Crossref
Added: February 25, 2021

2004 journal article

Interindividual variation in the metabolism of arsenic in cultured primary human hepatocytes

Toxicology and Applied Pharmacology, 201(2), 166–177.

By: Z. Drobná*, S. Waters*, F. Walton*, E. LeCluyse*, D. Thomas* & M. Stýblo*

author keywords: arsenic; metabolism; methylation; variation; human; hepatocyte; cyt19
MeSH headings : Adult; Aromatase / biosynthesis; Aromatase / genetics; Arsenic / metabolism; Cell Differentiation / drug effects; Cells, Cultured; Child, Preschool; Enzyme-Linked Immunosorbent Assay; Female; Genotype; Hepatocytes / metabolism; Humans; Infant; Male; Methylation; Middle Aged; Poisons / metabolism; RNA, Messenger / biosynthesis; Reverse Transcriptase Polymerase Chain Reaction
TL;DR: Genetic polymorphism of cyt19 along with other cellular factors is likely responsible for interindividual differences in the capacity of primary human hepatocytes to retain and metabolize iAs(III). (via Semantic Scholar)
Source: Crossref
Added: January 21, 2021

2004 journal article

Reversal of P-glycoprotein mediated vincristine resistance of L1210/VCR cells by analogues of pentoxifylline

European Journal of Pharmaceutical Sciences, 21(2-3), 283–293.

By: I. Kupsáková*, A. Rybár*, P. Dočolomanský*, Z. Drobná*, U. Stein*, W. Walther*, M. Barančı́k*, A. Breier*

author keywords: P-glycoprotein; L1210 cell line; multidrug resistance; xanthines; pentoxifylline analogues; vincristine; structure-activity relationship
MeSH headings : ATP Binding Cassette Transporter, Subfamily B, Member 1 / metabolism; Animals; Antineoplastic Agents, Phytogenic / chemistry; Antineoplastic Agents, Phytogenic / pharmacology; Cell Line, Tumor; Crystallization; Drug Resistance, Neoplasm / drug effects; Inhibitory Concentration 50; Leukemia L1210; Linear Models; Mice; Models, Biological; Pentoxifylline / analogs & derivatives; Pentoxifylline / chemistry; Pentoxifylline / pharmacology; Quantitative Structure-Activity Relationship; Vincristine / chemistry; Vincristine / pharmacology
TL;DR: The results indicate that for an effective reversal of P-gp mediated MDR of the authors' cells the existence of a longer polar substituent in the position N1 plays a crucial role and the molar refractivity influences the effectiveness of xanthine positively. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Source: Crossref
Added: January 21, 2021

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