@article{hetzler_marinakos_lott_mohammad_lass-napiorkowska_kolbe_turrentine_fields_overton_marie_et al._2024, title={Adeno-associated virus genome quantification with amplification-free CRISPR-Cas12a}, volume={3}, ISSN={["1476-5462"]}, DOI={10.1038/s41434-024-00449-x}, journal={GENE THERAPY}, author={Hetzler, Zach and Marinakos, Stella M. and Lott, Noah and Mohammad, Noor and Lass-Napiorkowska, Agnieszka and Kolbe, Jenna and Turrentine, Lauren and Fields, Delaney and Overton, Laurie and Marie, Helena and et al.}, year={2024}, month={Mar} }
 @article{jamalzadegan_kim_mohammad_koduri_hetzler_lee_dickey_wei_2024, title={Liquid Metal-Based Biosensors: Fundamentals and Applications}, volume={1}, ISSN={["1616-3028"]}, url={https://doi.org/10.1002/adfm.202308173}, DOI={10.1002/adfm.202308173}, abstractNote={Abstract}, journal={ADVANCED FUNCTIONAL MATERIALS}, author={Jamalzadegan, Sina and Kim, Sooyoung and Mohammad, Noor and Koduri, Harshita and Hetzler, Zach and Lee, Giwon and Dickey, Michael D. and Wei, Qingshan}, year={2024}, month={Jan} }
 @article{mohammad_talton_dalgan_hetzler_steksova_wei_2024, title={Ratiometric nonfluorescent CRISPR assay utilizing Cas12a-induced plasmid supercoil relaxation}, volume={7}, ISSN={["2399-3669"]}, DOI={10.1038/s42004-024-01214-2}, abstractNote={Most CRISPR-based biosensors rely on labeled reporter molecules and expensive equipment for signal readout. A recent approach quantifies analyte concentration by sizing λ DNA reporters via gel electrophoresis, providing a simple solution for label-free detection. Here, we report an alternative strategy for label-free CRISPR-Cas12a, which relies on Cas12a trans-nicking induced supercoil relaxation of dsDNA plasmid reporters to generate a robust and ratiometric readout. The ratiometric CRISPR (rCRISPR) measures the relative percentage of supercoiled plasmid DNA to the relaxed circular DNA by gel electrophoresis for more accurate target concentration quantification. This simple method is two orders of magnitude more sensitive than the typical fluorescent reporter. This self-referenced strategy solves the potential application limitations of previously demonstrated DNA sizing-based CRISPR-Dx without compromising the sensitivity. Finally, we demonstrated the applicability of rCRISPR for detecting various model DNA targets such as HPV 16 and real AAV samples, highlighting its feasibility for point-of-care CRISPR-Dx applications.}, number={1}, journal={COMMUNICATIONS CHEMISTRY}, author={Mohammad, Noor and Talton, Logan and Dalgan, Selen and Hetzler, Zach and Steksova, Anastasiia and Wei, Qingshan}, year={2024}, month={Jun} }
 @article{wang_sadeghi_velayati_paul_hetzler_danilov_ligler_wei_2023, title={Low-rate smartphone videoscopy for microsecond luminescence lifetime imaging with machine learning}, volume={2}, ISSN={["2752-6542"]}, url={https://doi.org/10.1093/pnasnexus/pgad313}, DOI={10.1093/pnasnexus/pgad313}, abstractNote={Abstract}, number={10}, journal={PNAS NEXUS}, author={Wang, Yan and Sadeghi, Sina and Velayati, Alireza and Paul, Rajesh and Hetzler, Zach and Danilov, Evgeny and Ligler, Frances S. and Wei, Qingshan}, editor={Reis, RuiEditor}, year={2023}, month={Sep} }
 @article{hetzler_lott_poonam_dalgan_wei_2023, title={Single-use biosensors for biomanufacturing: Perspective on the state-of-the-art}, volume={28}, ISSN={["2468-4511"]}, url={https://doi.org/10.1016/j.cobme.2023.100512}, DOI={10.1016/j.cobme.2023.100512}, abstractNote={The biomanufacturing industry has experienced significant transformations in the past 10 years, driven by changing industry trends and rapidly maturing new technologies. However, the pace of process monitoring technology development has lagged behind. This review focuses on the current major gaps in critical monitoring technology required for industry advancement, with a particular focus on single-use biosensors. There is a large unmet need for single-use biosensors in upstream and downstream processing as the industry transitions to single-use bioreactors. Emerging fields like cell and gene therapy that are still lacking numerous satisfactory at-line product characterization sensors will also be covered.}, journal={CURRENT OPINION IN BIOMEDICAL ENGINEERING}, author={Hetzler, Zach and Lott, Noah and Poonam, Aditi Dey and Dalgan, Selen and Wei, Qingshan}, year={2023}, month={Dec} }
 @article{mohammad_talton_hetzler_gongireddy_wei_2023, title={Unidirectional trans-cleaving behavior of CRISPR-Cas12a unlocks for an ultrasensitive assay using hybrid DNA reporters containing a 3' toehold}, volume={8}, ISSN={["1362-4962"]}, url={https://doi.org/10.1093/nar/gkad715}, DOI={10.1093/nar/gkad715}, abstractNote={Abstract}, journal={NUCLEIC ACIDS RESEARCH}, author={Mohammad, Noor and Talton, Logan and Hetzler, Zach and Gongireddy, Megha and Wei, Qingshan}, year={2023}, month={Aug} }
 @article{hetzler_wang_krafft_jamalzadegan_overton_kudenov_ligler_wei_2022, title={Flexible sensor patch for continuous carbon dioxide monitoring}, volume={10}, ISSN={["2296-2646"]}, DOI={10.3389/fchem.2022.983523}, abstractNote={Monitoring and measurement of carbon dioxide (CO2) is critical for many fields. The gold standard CO2 sensor, the Severinghaus electrode, has remained unchanged for decades. In recent years, many other CO2 sensor formats, such as detection based upon pH-sensitive dyes, have been demonstrated, opening the door for relatively simple optical detection schemes. However, a majority of these optochemical sensors require complex sensor preparation steps and are difficult to control and repeatably execute. Here, we report a facile CO2 sensor generation method that suffers from none of the typical fabrication issues. The method described here utilizes polydimethylsiloxane (PDMS) as the flexible sensor matrix and 1-hydroxypyrene-3,6,8-trisulfonate (HPTS), a pH-sensitive dye, as the sensing material. HPTS, a base (NaOH), and glycerol are loaded as dense droplets into a thin PDMS layer which is subsequently cured around the droplet. The fabrication process does not require prior knowledge in chemistry or device fabrication and can be completed as quickly as PDMS cures (∼2 h). We demonstrate the application of this thin-patch sensor for in-line CO2 quantification in cell culture media. To this end, we optimized the sensing composition and quantified CO2 in the range of 0–20 kPa. A standard curve was generated with high fidelity (R2 = 0.998) along with an analytical resolution of 0.5 kPa (3.7 mm Hg). Additionally, the sensor is fully autoclavable for applications requiring sterility and has a long working lifetime. This flexible, simple-to-manufacture sensor has a myriad of potential applications and represents a new, straightforward means for optical carbon dioxide measurement.}, journal={FRONTIERS IN CHEMISTRY}, author={Hetzler, Zach and Wang, Yan and Krafft, Danny and Jamalzadegan, Sina and Overton, Laurie and Kudenov, Michael W. and Ligler, Frances S. and Wei, Qingshan}, year={2022}, month={Sep} }