Zidan Li

College of Engineering

Works (5)

Updated: April 5th, 2024 15:09

2024 article

Chips, guts, and gas: unraveling volatile microbial mysteries in real time!

Li, Z., & Crook, N. C. (2024, February). TRENDS IN BIOTECHNOLOGY, Vol. 42, pp. 144–146.

By: Z. Li n & N. Crook n

TL;DR: The role of hydrogen sulfide in the gastrointestinal role is explored by engineering Escherichia coli to titrate H2S levels in a gas-impermeable gut-on-chip device. (via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: December 29, 2023

2023 article

Engineering the Maize Root Microbiome: A Rapid MoClo Toolkit and Identification of Potential Bacterial Chassis for Studying Plant-Microbe Interactions

Schaik, J., Li, Z., Cheadle, J., & Crook, N. (2023, September 15). ACS SYNTHETIC BIOLOGY, Vol. 9.

By: J. Schaik n, Z. Li n, J. Cheadle n & N. Crook n

author keywords: modular cloning; synthetic community; maize; promoter trapping; electroporation; toolkit
Sources: Web Of Science, ORCID, NC State University Libraries
Added: September 16, 2023

2023 journal article

Improving therapeutic protein secretion in the probiotic yeast Saccharomyces boulardii using a multifactorial engineering approach


MeSH headings : Saccharomyces cerevisiae / genetics; Saccharomyces cerevisiae / metabolism; Saccharomyces boulardii / genetics; Saccharomyces boulardii / metabolism; Saccharomyces / genetics; Saccharomyces / metabolism; Probiotics / metabolism; Endopeptidases / metabolism
TL;DR: A quadruple protease-deficient Sb strain is constructed that produces 5045 mg/L secretory NPA, an improvement of tenfold over wild-type Sb and highlights the ability of proteomics to highlight under-explored mediators of this process. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: June 19, 2023

2022 journal article

A Novel Method of Inducible Directed Evolution to Evolve Complex Phenotypes


By: I. Al'Abri*, Z. Li*, D. Haller* & N. Crook*

author keywords: Directed Evolution; Inducible Systems; Complex Phenotypes; Pathways; Gene Clusters; P1 Phage; Mutagenesis
TL;DR: A new directed evolution method (Inducible Directed Evolution, IDE) is developed that combines the specificity and throughput of recent continuous directed evolution methods with the ease of ALE, which enables long DNA sequences to be mutated in a high throughput manner via a simple series of incubation steps. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: June 12, 2023

2022 article

Inducible directed evolution of complex phenotypes in bacteria

Al'Abri, I. S., Haller, D. J., Li, Z., & Crook, N. (2022, February 12). NUCLEIC ACIDS RESEARCH, Vol. 2.

By: I. Al'Abri n, D. Haller n, Z. Li n & N. Crook n

MeSH headings : Bacteria / genetics; Bacteria / metabolism; Bacteria / virology; Bacteriophages / genetics; Directed Molecular Evolution / methods; Mutagenesis; Phenotype; Plasmids / genetics
Sources: Web Of Science, ORCID, NC State University Libraries
Added: February 13, 2022

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