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Works (5)
Updated: April 5th, 2024 15:09
2023 article
Chips, guts, and gas: unraveling volatile microbial mysteries in real time!
Li, Z., & Crook, N. C. (2024, February). TRENDS IN BIOTECHNOLOGY, Vol. 42, pp. 144–146.
TL;DR:
The role of hydrogen sulfide in the gastrointestinal role is explored by engineering Escherichia coli to titrate H2S levels in a gas-impermeable gut-on-chip device.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: December 29, 2023
2023 article
Engineering the Maize Root Microbiome: A Rapid MoClo Toolkit and Identification of Potential Bacterial Chassis for Studying Plant-Microbe Interactions
Schaik, J., Li, Z., Cheadle, J., & Crook, N. (2023, September 15). ACS SYNTHETIC BIOLOGY, Vol. 9.
author keywords: modular cloning; synthetic community; maize; promoter trapping; electroporation; toolkit
open access via doi.org (repository)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: September 16, 2023
2023 journal article
Improving therapeutic protein secretion in the probiotic yeast Saccharomyces boulardii using a multifactorial engineering approach
MICROBIAL CELL FACTORIES, 22(1).
Contributors: D. Durmusoglu n, I. Al’Abri n, n, T. Islam Williams n, L. Collins n , J. Martínez *, N. Crook n
MeSH headings : Saccharomyces cerevisiae / genetics; Saccharomyces cerevisiae / metabolism; Saccharomyces boulardii / genetics; Saccharomyces boulardii / metabolism; Saccharomyces / genetics; Saccharomyces / metabolism; Probiotics / metabolism; Endopeptidases / metabolism
TL;DR:
A quadruple protease-deficient Sb strain is constructed that produces 5045 mg/L secretory NPA, an improvement of tenfold over wild-type Sb and highlights the ability of proteomics to highlight under-explored mediators of this process.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: June 19, 2023
2022 journal article
A Novel Method of Inducible Directed Evolution to Evolve Complex Phenotypes
BIO-PROTOCOL, 12(20).
author keywords: Directed Evolution; Inducible Systems; Complex Phenotypes; Pathways; Gene Clusters; P1 Phage; Mutagenesis
TL;DR:
A new directed evolution method (Inducible Directed Evolution, IDE) is developed that combines the specificity and throughput of recent continuous directed evolution methods with the ease of ALE, which enables long DNA sequences to be mutated in a high throughput manner via a simple series of incubation steps.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: June 12, 2023
2022 article
Inducible directed evolution of complex phenotypes in bacteria
Al'Abri, I. S., Haller, D. J., Li, Z., & Crook, N. (2022, February 12). NUCLEIC ACIDS RESEARCH, Vol. 2.
MeSH headings : Bacteria / genetics; Bacteria / metabolism; Bacteria / virology; Bacteriophages / genetics; Directed Molecular Evolution / methods; Mutagenesis; Phenotype; Plasmids / genetics
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: February 13, 2022
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