Zidan Li

Works (6)

Updated: August 30th, 2025 05:03

2025 article

Inducible genome-wide mutagenesis for improvement of pDNA production by E. coli

Li, Z., Sun, G., Al’Abri, I., Zhou, Y., & Crook, N. (2025, August 13). Microbial Cell Factories, Vol. 24.

By: Z. Li n, G. Sun n, I. Al’Abri n, Y. Zhou  n & N. Crook* 

author keywords: Plasmid DNA; <italic>Escherichia coli</italic>; Strain engineering; Genome mutagenesis
topics (OpenAlex): Bacteriophages and microbial interactions; CRISPR and Genetic Engineering; Bacterial Genetics and Biotechnology
Sources: Web Of Science, NC State University Libraries
Added: August 25, 2025

2023 article

Chips, guts, and gas: unraveling volatile microbial mysteries in real time!

Li, Z., & Crook, N. C. (2023, December 28). Trends in Biotechnology, Vol. 42, pp. 144–146.

By: Z. Li n & N. Crook n 

MeSH headings : Hydrogen Sulfide; Gastrointestinal Tract; Escherichia coli / genetics
topics (OpenAlex): 3D Printing in Biomedical Research; Advanced Chemical Sensor Technologies; Diet and metabolism studies
TL;DR: The role of hydrogen sulfide in the gastrointestinal role is explored by engineering Escherichia coli to titrate H2S levels in a gas-impermeable gut-on-chip device. (via Semantic Scholar)
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Sources: Web Of Science, ORCID, NC State University Libraries
Added: December 29, 2023

2023 article

Engineering the Maize Root Microbiome: A Rapid MoClo Toolkit and Identification of Potential Bacterial Chassis for Studying Plant–Microbe Interactions

Schaik, J., Li, Z., Cheadle, J., & Crook, N. (2023, September 15). ACS Synthetic Biology, Vol. 9.

By: J. Schaik n, Z. Li n, J. Cheadle n  & N. Crook n 

author keywords: modular cloning; synthetic community; maize; promoter trapping; electroporation; toolkit
MeSH headings : Zea mays / genetics; Zea mays / microbiology; Bacteria / genetics; Plasmids / genetics; Genetic Engineering / methods; Microbiota / genetics
topics (OpenAlex): Plant-Microbe Interactions and Immunity; Legume Nitrogen Fixing Symbiosis; Microbial Community Ecology and Physiology; Plant tissue culture and regeneration
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Sources: Web Of Science, ORCID, NC State University Libraries
Added: September 16, 2023

2023 article

Improving therapeutic protein secretion in the probiotic yeast Saccharomyces boulardii using a multifactorial engineering approach

Durmusoglu, D., Al’Abri, I., Li, Z., Williams, T. I., Collins, L. B., Martínez, J. L., & Crook, N. (2023, June 7). Microbial Cell Factories, Vol. 22.

By: D. Durmusoglu n, I. Al’Abri n, Z. Li n, T. Williams n , L. Collins n , J. Martínez *, N. Crook n 

Contributors: D. Durmusoglu n, I. Al’Abri n, Z. Li n, T. Williams n , L. Collins n , J. Martínez *, N. Crook n 

MeSH headings : Saccharomyces cerevisiae / genetics; Saccharomyces cerevisiae / metabolism; Saccharomyces boulardii / genetics; Saccharomyces boulardii / metabolism; Saccharomyces / genetics; Saccharomyces / metabolism; Probiotics / metabolism; Endopeptidases / metabolism
topics (OpenAlex): Fungal and yeast genetics research; Bacterial Genetics and Biotechnology; Microbial Inactivation Methods; Transgenic Plants and Applications; Microbial Metabolic Engineering and Bioproduction
TL;DR: A quadruple protease-deficient Sb strain is constructed that produces 5045 mg/L secretory NPA, an improvement of tenfold over wild-type Sb and highlights the ability of proteomics to highlight under-explored mediators of this process. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: June 19, 2023

2022 article

A Novel Method of Inducible Directed Evolution to Evolve Complex Phenotypes

Al'Abri, I., Li, Z., Haller, D., & Crook, N. (2022, January 1). BIO-PROTOCOL, Vol. 12.

By: I. Al'Abri n, Z. Li n, D. Haller n & N. Crook n 

author keywords: Directed Evolution; Inducible Systems; Complex Phenotypes; Pathways; Gene Clusters; P1 Phage; Mutagenesis
topics (OpenAlex): Genomics and Phylogenetic Studies; CRISPR and Genetic Engineering; Bacteriophages and microbial interactions
TL;DR: A new directed evolution method (Inducible Directed Evolution, IDE) is developed that combines the specificity and throughput of recent continuous directed evolution methods with the ease of ALE, which enables long DNA sequences to be mutated in a high throughput manner via a simple series of incubation steps. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: June 12, 2023

2022 article

Inducible directed evolution of complex phenotypes in bacteria

Al’Abri, I. S., Haller, D. J., Li, Z., & Crook, N. (2022, February 1). Nucleic Acids Research, Vol. 2.

By: I. Al’Abri n, D. Haller n, Z. Li n & N. Crook n 

MeSH headings : Bacteria / genetics; Bacteria / metabolism; Bacteria / virology; Bacteriophages / genetics; Directed Molecular Evolution / methods; Mutagenesis; Phenotype; Plasmids / genetics
topics (OpenAlex): CRISPR and Genetic Engineering; Bacteriophages and microbial interactions; Polyamine Metabolism and Applications
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Sources: Web Of Science, ORCID, NC State University Libraries
Added: February 13, 2022

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