@article{park_wang_lee_jameel_jin_park_2016, title={Effect of the Two-Stage Autohydrolysis of Hardwood on the Enzymatic Saccharification and Subsequent Fermentation with an Efficient Xylose-Utilizing Saccharomyces cerevisiae}, volume={11}, ISSN={["1930-2126"]}, DOI={10.15376/biores.11.4.9584-9595}, abstractNote={To effectively utilize sugars during the fermentation process, it is important to develop a process that can minimize the generation of inhibiting compounds such as furans and acids, and a robust micro-organism that can co-ferment both glucose and xylose into products. In this study, the feasibility of efficient ethanol production was investigated using a combination of two approaches: two-stage autohydrolysis of biomass and fermentation using an engineered Saccharomyces cerevisiae to produce ethanol. When the hardwood chips were autohydrolyzed at 140 °C, followed by the second treatment at 180 °C, a higher yield of sugar conversion and fewer inhibitory effects on subsequent fermentation were achieved compared with the results from single-stage autohydrolysis. A higher overall yield of ethanol resulted by using an engineered yeast strain, SR8. This observation suggests the possibility of the feasible combination of two-stage autohydrolysis and the recombinant yeast.}, number={4}, journal={BIORESOURCES}, author={Park, Junyeong and Wang, Ziyu and Lee, Won-Heong and Jameel, Hasan and Jin, Yong-Su and Park, Sunkyu}, year={2016}, month={Nov}, pages={9584–9595} }
 @article{zu_li_zhang_li_wang_jameel_chang_2014, title={Pretreatment of corn stover for sugar production using dilute hydrochloric acid followed by lime}, volume={152}, ISSN={["1873-2976"]}, DOI={10.1016/j.biortech.2013.11.034}, abstractNote={In this study, a two stage process was evaluated to increase the sugar recovery. Firstly, corn stover was treated with diluted hydrochloric acid to maximize the xylose yield, and then the residue was treated with lime to alter the lignin structure and swell the cellulose surface. The optimal condition was 120 °C and 40 min for diluted hydrochloric acid pretreatment followed by lime pretreatment at 60 °C for 12h with lime loading at 0.1 g/g of substrate. The glucose and xylose yield was 78.0% and 97.0%, respectively, with cellulase dosage at 5 FPU/g of substrate. The total glucose yield increased to 85.9% when the cellulase loading was increased to 10 FPU/g of substrate. This two stage process was effective due to the swelling of the internal surface, an increase in the porosity and a decrease in the degree of polymerization.}, journal={BIORESOURCE TECHNOLOGY}, author={Zu, Shuai and Li, Wen-zhi and Zhang, Mingjian and Li, Zihong and Wang, Ziyu and Jameel, Hasan and Chang, Hou-min}, year={2014}, month={Jan}, pages={364–370} }
 @article{zhang_li_zu_huo_zhu_wang_2013, title={Catalytic hydrogenation for bio-oil upgrading by a supported NiMoB amorphous alloy}, volume={36}, number={12}, journal={Chemical Engineering & Technology}, author={Zhang, M. J. and Li, W. Z. and Zu, S. and Huo, W. and Zhu, X. F. and Wang, Z. Y.}, year={2013}, pages={2108–2116} }
 @article{he_ji_cheng_wang_qian_li_gong_wang_2013, title={Structural characterization and immunostimulatory activity of a novel protein-bound polysaccharide produced by Hirsutella sinensis Liu, Guo, Yu & Zeng}, volume={141}, number={2}, journal={Food Chemistry}, author={He, L. and Ji, P. F. and Cheng, J. W. and Wang, Y. B. and Qian, H. and Li, W. Q. and Gong, X. G. and Wang, Z. Y.}, year={2013}, pages={946–953} }
 @article{zhou_xu_wang_cheng_li_qu_2012, title={Dilute sulfuric acid pretreatment of transgenic switchgrass for sugar production}, volume={104}, ISSN={["0960-8524"]}, DOI={10.1016/j.biortech.2011.11.051}, abstractNote={Conventional Alamo switchgrass and its transgenic counterparts with reduced/modified lignin were subjected to dilute sulfuric acid pretreatment for improved sugar production. At 150 °C, the effects of acid concentration (0.75%, 1%, 1.25%) and residence time (5, 10, 20, 30 min) on sugar productions in pretreatment and enzymatic hydrolysis were investigated, with the optimal pretreatment conditions determined for each switchgrass genotype based on total sugar yield and the amounts of sugar degradation products generated during the pretreatment. The results show that genetic engineering, although did not cause an appreciable lignin reduction, resulted in a substantial increase in the ratio of acid soluble lignin:acid insoluble lignin, which led to considerably increased sugar productions in both pretreatment and enzymatic hydrolysis. At an elevated threshold concentration of combined 5-hydroxyfuranmethal and furfural (2.0 g/L), the overall carbohydrate conversions of conventional switchgrass and its transgenic counterparts, 10/9-40 and 11/5-47, reached 75.9%, 82.6%, and 82.2%, respectively.}, journal={BIORESOURCE TECHNOLOGY}, author={Zhou, Xu and Xu, Jiele and Wang, Ziyu and Cheng, Jay J. and Li, Ruyu and Qu, Rongda}, year={2012}, month={Jan}, pages={823–827} }
 @article{wang_xu_pandey_cheng_li_qu_2012, title={Improvement of Sugar Production from Transgenic Switchgrass with Low-Temperature Alkali Pretreatment}, volume={26}, ISSN={["1520-5029"]}, DOI={10.1021/ef3004575}, abstractNote={Genetically modified switchgrass (cv. Alamo) and its conventional plant were both pretreated using two groups of conditions: lime at 50 °C and the combination of lime and NaOH at ambient temperature. The results show that the transgenic plant (with altered lignin content and composition) was more susceptible to alkali pretreatment than the conventional plant. At the recommended conditions (0.1 g/g of raw biomass and 12 h) for lime pretreatment at 50 °C, the glucan and xylan conversions of transgenic switchgrass were 12 and 10%, respectively, higher than those of the conventional plant. These increases were reduced to 7 and 8% for glucan and xylan conversions, respectively, when the best conditions (0.025 g of lime/g of raw biomass, 0.1 g of NaOH/g of raw biomass, and 6 h) for combined alkali pretreatment at ambient temperature were employed. The advantage of transgenics over a conventional plant in sugar production could be maximized if proper pretreatment conditions were used.}, number={5}, journal={ENERGY & FUELS}, author={Wang, Ziyu and Xu, Jiele and Pandey, Pankaj and Cheng, Jay J. and Li, Ruyu and Qu, Rongda}, year={2012}, month={May}, pages={3054–3061} }
 @misc{xu_wang_cheng_2011, title={Bermuda grass as feedstock for biofuel production: A review}, volume={102}, ISSN={["1873-2976"]}, DOI={10.1016/j.biortech.2011.05.070}, abstractNote={Bermuda grass is a promising feedstock for the production of fuel ethanol in the Southern United States. This paper presents a review of the significant amount of research on the conversion of Bermuda grass to ethanol and a brief discussion on the factors affecting the biomass production in the field. The biggest challenge of biomass conversion comes from the recalcitrance of lignocellulose. A variety of chemical, physico-chemical, and biological pretreatment methods have been investigated to improve the digestibility of Bermuda grass with encouraging results reported. The subsequent enzymatic hydrolysis and fermentation steps have also been extensively studied and effectively optimized. It is expected that the development of genetic engineering technologies for the grass and fermenting organisms has the potential to greatly improve the economic viability of Bermuda grass-based fuel ethanol production systems. Other energy applications of Bermuda grass include anaerobic digestion for biogas generation and pyrolysis for syngas production.}, number={17}, journal={BIORESOURCE TECHNOLOGY}, author={Xu, Jiele and Wang, Ziyu and Cheng, Jay J.}, year={2011}, month={Sep}, pages={7613–7620} }
 @article{xu_wang_sharma-shivappa_cheng_2011, title={Enzymatic hydrolysis of switchgrass and coastal bermuda grass pretreated using different chemical methods}, volume={6}, number={3}, journal={BioResources}, author={Xu, J. L. and Wang, Z. Y. and Sharma-Shivappa, R. R. and Cheng, J. J.}, year={2011}, pages={2990–3003} }
 @article{redding_wang_keshwani_cheng_2011, title={High temperature dilute acid pretreatment of coastal Bermuda grass for enzymatic hydrolysis}, volume={102}, ISSN={["1873-2976"]}, DOI={10.1016/j.biortech.2010.09.053}, abstractNote={Dilute sulfuric acid was used to pretreat coastal Bermuda grass at high temperature prior to enzymatic hydrolysis. After both pretreatment and enzymatic hydrolysis processes, the highest yield of total sugars (combined xylose and glucose) was 97% of the theoretical value. The prehydrolyzate liquor was analyzed for inhibitory compounds (furfural, hydroxymethylfurfural (HMF)) in order to assess potential risk for inhibition during the following fermentation. Accounting for the formation of the inhibitory compounds, a pretreatment with 1.2% acid at 140 °C for 30 min with a total sugar yield of 94% of the theoretical value may be more favorable for fermentation. From this study, it can be concluded that dilute sulfuric acid pretreatment can be successfully applied to coastal Bermuda grass to achieve high yields of monomeric glucose and xylose with acceptable levels of inhibitory compound formation.}, number={2}, journal={BIORESOURCE TECHNOLOGY}, author={Redding, Arthur P. and Wang, Ziyu and Keshwani, Deepak R. and Cheng, Jay J.}, year={2011}, month={Jan}, pages={1415–1424} }
 @article{wang_cheng_2011, title={Lime Pretreatment of Coastal Bermudagrass for Bioethanol Production}, volume={25}, ISSN={["1520-5029"]}, DOI={10.1021/ef2000932}, abstractNote={Coastal bermudagrass (CBG) is regarded as a potential lignocellulosic feedstock for bioethanol production in the southeast United States. Lime pretreatment of CBG for enhanced reducing sugar recovery was investigated in this study, which examined a variety of temperatures (21−121 °C) at a range of residence times with different lime loadings (0.02−0.20 g/g of dry biomass). During pretreatment, 10−20% lignin was removed. After enzymatic hydrolysis with excessive cellulases and cellobiase, the best total reducing sugar yield for the lime-pretreated CBG was 78% of the theoretical maximum, which is over 2 times more than that from the untreated CBG. The recommended condition is 100 °C for 15 min with a lime loading of 0.1 g/g of dry biomass, under which 87% glucan and 68% xylan were converted to glucose and xylose, respectively. Fermentation tests of the hydrolyzates indicated that more than 99% glucose in the hydrolyzate was used by the yeast during the fermentation, with ethanol yields of 95% of the theoret...}, number={4}, journal={ENERGY & FUELS}, author={Wang, Ziyu and Cheng, Jay J.}, year={2011}, month={Apr}, pages={1830–1836} }
 @misc{wang_xu_cheng_2011, title={Modeling biochemical conversion of lignocellulosic materials for sugar production: a Review}, volume={6}, number={4}, journal={BioResources}, author={Wang, Z. Y. and Xu, J. L. and Cheng, J. J.}, year={2011}, pages={5282–5306} }
 @article{wang_keshwani_redding_cheng_2010, title={Sodium hydroxide pretreatment and enzymatic hydrolysis of coastal Bermuda grass}, volume={101}, ISSN={["1873-2976"]}, DOI={10.1016/j.biortech.2009.12.097}, abstractNote={Coastal Bermuda grass was pretreated with NaOH at concentrations from 0.5% to 3% (w/v) for a residence time from 15 to 90 min at 121 °C. The pretreatments were evaluated based on total lignin removal and production of total reducing sugars, glucose and xylose from enzymatic hydrolysis of the pretreated biomass. Up to 86% lignin removal was observed. The optimal NaOH pretreatment conditions at 121 °C for total reducing sugars production as well as glucose and xylose yields are 15 min and 0.75% NaOH. Under these optimal pretreatment conditions, total reducing sugars yield was about 71% of the theoretical maximum, and the overall conversion efficiencies for glucan and xylan were 90.43% and 65.11%, respectively.}, number={10}, journal={BIORESOURCE TECHNOLOGY}, author={Wang, Ziyu and Keshwani, Deepak R. and Redding, Arthur P. and Cheng, Jay J.}, year={2010}, month={May}, pages={3583–3585} }