2023 article

Manipulating Fermentation Pathways in the Hyperthermophilic Archaeon Pyrococcus furiosus for Ethanol Production up to 95 degrees C Driven by Carbon Monoxide Oxidation

Lipscomb, G. L., Crowley, A. T., Nguyen, D. M. N., Keller, M. W., Hailey C. O'Quinn, Tanwee, T. N. N., … Adams, M. W. W. (2023, May 10). APPLIED AND ENVIRONMENTAL MICROBIOLOGY.

By: G. Lipscomb*, A. Crowley*, D. Nguyen *, M. Keller *, . Hailey C. O'Quinn*, T. Tanwee *, J. Vailionis *, K. Zhang * ...

author keywords: Pyrococcus furiosus; archaea; thermophile; ethanol; genetic engineering; CO dehydrogenase; bioethanol; carbon monoxide; hyperthermophiles; metabolic engineering; metabolic modeling
TL;DR: An engineered strain of P. furiosus is described that synthesizes ethanol at 95°C, utilizing a homologously-expressed native alcohol dehydrogenase, termed AdhF, and opens the door to the potential for ‘bioreactive distillation’ since fermentation can be performed well above the normal boiling point of ethanol. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: May 22, 2023

Previously, the highest temperature for biological ethanol production was 85°C. Here, we have engineered ethanol production at 95°C by the hyperthermophilic archaeon Pyrococcus furiosus .