2015 journal article

Directed Enzymatic Activation of 1-D DNA Tiles

ACS NANO, 9(2), 1072–1079.

By: S. Garg*, H. Chandran*, N. Gopalkrishnan*, T. LaBean n & J. Reif*

author keywords: DNA self assembly; DNA Polymerase; DNA tile; directed assembly; sequential assembly
MeSH headings : DNA / chemistry; DNA-Directed DNA Polymerase / metabolism; Enzyme Activation; Nanostructures / chemistry; Nanotechnology / methods
TL;DR: A simplified version of the Activatable tile assembly model is experimentally demonstrate the simultaneous assembly of protected DNA tiles and stepwise activated assembly where a set of inert tiles are activated sequentially one after another as a result of attachment to a growing 1-D assembly. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

The tile assembly model is a Turing universal model of self-assembly where a set of square shaped tiles with programmable sticky sides undergo coordinated self-assembly to form arbitrary shapes, thereby computing arbitrary functions. Activatable tiles are a theoretical extension to the Tile assembly model that enhances its robustness by protecting the sticky sides of tiles until a tile is partially incorporated into a growing assembly. In this article, we experimentally demonstrate a simplified version of the Activatable tile assembly model. In particular, we demonstrate the simultaneous assembly of protected DNA tiles where a set of inert tiles are activated via a DNA polymerase to undergo linear assembly. We then demonstrate stepwise activated assembly where a set of inert tiles are activated sequentially one after another as a result of attachment to a growing 1-D assembly. We hope that these results will pave the way for more sophisticated demonstrations of activated assemblies.