2022 journal article
Mechanisms and modeling of wound repair in the intestinal epithelium
Tissue Barriers.
author keywords: IPEC-J2 cells; transepithelial electrical resistance; injury barrier function; cell model
MeSH headings : Humans; Caco-2 Cells; Intestinal Mucosa / metabolism; Intestines; Ischemia
TL;DR:
This review focuses on in vitro injury models and intestinal cell lines utilized in such systems and the use of intestine cell lines, IPEC-J2, Caco-2, T-84, HT-29, and IEC-6, to model intestinal epithelium.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Source: ORCID
Added: June 12, 2022
ABSTRACT The intestinal epithelial barrier is susceptible to injury from insults, such as ischemia or infectious disease. The epithelium’s ability to repair wounded regions is critical to maintaining barrier integrity. Mechanisms of intestinal epithelial repair can be studied with models that recapitulate the in vivo environment. This review focuses on in vitro injury models and intestinal cell lines utilized in such systems. The formation of artificial wounds in a controlled environment allows for the exploration of reparative physiology in cell lines modeling diverse aspects of intestinal physiology. Specifically, the use of intestinal cell lines, IPEC-J2, Caco-2, T-84, HT-29, and IEC-6, to model intestinal epithelium is discussed. Understanding the unique systems available for creating intestinal injury and the differences in monolayers used for in vitro work is essential for designing studies that properly capture relevant physiology for the study of intestinal wound repair.