2016 article
Histological Analysis of the Arabidopsis Gynoecium and Ovules Using Chloral Hydrate Clearing and Differential Interference Contrast Light Microscopy
OOGENESIS: METHODS AND PROTOCOLS, Vol. 1457, pp. 1–7.
author keywords: Ovule; Chloral hydrate; Nomarski optics; Ovary; Seed development; Seedpod
MeSH headings : Arabidopsis / cytology; Arabidopsis / physiology; Chloral Hydrate; Cytological Techniques; Microscopy, Interference; Ovule / cytology
TL;DR:
The use of chloral hydrate optical clearing paired with differential interference contrast microscopy allows the analysis of internal structures of developing plant organs without the need for paraffin embedding and sectioning.
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Added: August 6, 2018
The use of chloral hydrate optical clearing paired with differential interference contrast microscopy allows the analysis of internal structures of developing plant organs without the need for paraffin embedding and sectioning. This approach is appropriate for the analysis of the developing gynoecium or seedpod of the flowering plant Arabidopsis thaliana and many other types of fixed plant material. Early stages of ovule development are observable with this approach.