Banana fibers are a sustainable material with natural mechanical strength and antibacterial properties. These fibers are extracted from the large amount of waste produced by banana pseudo stems annually. However, despite their numerous advantages, their stiffness and rough texture impede their full use in the textile. This research investigates the degumming treatment of banana fibers using enzyme combination and chemical methods to achieve spinnable soft banana fibers. An L9 orthogonal array was used in a Taguchi design of the experiment to optimize the process parameters. For enzyme combination degumming, the experimental setup comprised different quantities of hemicellulase, laccase, amylase, and pectinase; for chemical degumming, varied amounts of sodium hydroxide (NaOH) were used. The results indicate that enzyme-based degumming procedures produce better results than chemical treatments. Optimum enzyme combinations for various fiber qualities were found using the Taguchi design of experiments. These combinations included Hemicellulase 5 %, Laccase 5 %, Amylase 3 %, and Hemicellulase 5 %, Laccase 3 %, Pectinase 5 %. Without degrading the cellulose structure, these ideal enzyme combinations produced fibers with lower lignin content and higher cellulose percentages, moisture content, and tenacity values. By determining the most efficient enzyme combinations and their effects on fiber qualities, the study offers sustainable fiber processing methods for textile grade banana fiber.