2017 journal article
Response to Zambon et al.
Current Biology, 27(3), R101–R102.
MeSH headings : Constriction; Myosin Heavy Chains; Myosin Type II; Myosins; Schizosaccharomyces; Schizosaccharomyces pombe Proteins
TL;DR:
It is concluded that Myo2p is the major motor involved in ring contraction in S. pombe and most of the differences observed by Zambon et al. can be attributed to their use of the Rlc1p-3GFP marker, which genetically interacts with myo2-E1.
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open access via www.cell.com (publisher PDF)
Source: Crossref
Added: February 21, 2020
Stimulated by our 2015 Current Biology paper [1Laplante C. Berro J. Karatekin E. Hernandez-Leyva A. Lee R. Pollard T.D. Three myosins contribute uniquely to the assembly and constriction of the fission yeast cytokinetic contractile ring.Curr. Biol. 2015; 25: 1955-1965Abstract Full Text Full Text PDF PubMed Scopus (48) Google Scholar], Zambon et al. reinvestigated how three myosin isoforms participate in the formation and constriction of the contractile ring in fission yeast. Our paper presented evidence that these myosin isoforms have distinct roles: “Conventional myosin-II Myo2 is crucial to ring assembly, unconventional myosin-II Myp2 is most important for ring constriction, and type V myosin Myo51 aids the other two myosins.” Zambon et al. used different markers to reexamine the contributions of the three myosins to cytokinesis and concluded “that Myo2p is the major motor involved in ring contraction in S. pombe.” Here, we show that most of the differences observed by Zambon et al. can be attributed to their use of the Rlc1p-3GFP marker, which genetically interacts with myo2-E1.