TL;DR:
Immunohistochemistry in combination with 3D reconstruction of centers of rosette indicated that epitopes of the xyloglucan-specific antibody LM25 were highly abundant in the vasculature of Col-0 plants after sub-zero acclimation but absent in the XTH19 mutant, shedding new light on the potential roles of cell wall remodeling for the increased freezing tolerance observed after low temperature acclimations.
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