2022 journal article
Tissue Culture and Regeneration of Three Rose Cultivars
HORTSCIENCE, 57(11), 1430–1435.
Methods of in vitro regeneration protocols were developed for three elite rose cultivars, Chewnicebell (Oso Easy Italian Ice ® ), Bucbi (Carefree Beauty™), and Cheweyesup (Ringo All-Star™). We evaluated the effects of different types and concentrations of auxins [dichlorophenoxyacetic acid (2,4-D) and trichlorophenoxyacetic acid (2,4,5-T)], carbohydrates [sucrose, glucose, and fructose], and cytokinins [thidiazuron (TDZ) and 6-bezylaminopurine (BAP)] on callus induction and regeneration from leaf explants. The greatest amount of regenerative callus was obtained on media containing 10 µM 2,4-D and 30 g·L −1 sucrose for Italian Ice ® (40%), 10 µM 2,4-D and 60 g·L −1 glucose for Carefree Beauty™ (24%), and 5 µM 2,4,5-T and 30 g·L −1 sucrose for Ringo All-Star™ (32%). The greatest regeneration occurred when callus was transferred to media consisting of 1/2 MS media supplemented with 2.9 µM GA 3 and 5 µM TDZ for Italian Ice ® and Ringo All-Star™, and with 2.9 µM GA 3 and 20 µM TDZ for Carefree Beauty™. Plantlets regenerated from callus were cultured on maintenance media and successfully transferred ex vitro. This study highlights the genotype-specific responses among rose cultivars and provides the first reports of in vitro regeneration for Italian Ice ® and Ringo All-Star™.