2022 journal article

Tissue Culture and Regeneration of Three Rose Cultivars

HORTSCIENCE, 57(11), 1430–1435.

By: D. Harmon n, D. Touchell n, T. Ranney n, K. Da n & W. Liu n

co-author countries: United States of America 🇺🇸
author keywords: auxin; carbohydrate; cytokinin; plant regeneration; micropropagation; somatic embryogenesis; Rosa ?hybrida
Source: Web Of Science
Added: November 7, 2022

Methods of in vitro regeneration protocols were developed for three elite rose cultivars, Chewnicebell (Oso Easy Italian Ice ® ), Bucbi (Carefree Beauty™), and Cheweyesup (Ringo All-Star™). We evaluated the effects of different types and concentrations of auxins [dichlorophenoxyacetic acid (2,4-D) and trichlorophenoxyacetic acid (2,4,5-T)], carbohydrates [sucrose, glucose, and fructose], and cytokinins [thidiazuron (TDZ) and 6-bezylaminopurine (BAP)] on callus induction and regeneration from leaf explants. The greatest amount of regenerative callus was obtained on media containing 10 µM 2,4-D and 30 g·L −1 sucrose for Italian Ice ® (40%), 10 µM 2,4-D and 60 g·L −1 glucose for Carefree Beauty™ (24%), and 5 µM 2,4,5-T and 30 g·L −1 sucrose for Ringo All-Star™ (32%). The greatest regeneration occurred when callus was transferred to media consisting of 1/2 MS media supplemented with 2.9 µM GA 3 and 5 µM TDZ for Italian Ice ® and Ringo All-Star™, and with 2.9 µM GA 3 and 20 µM TDZ for Carefree Beauty™. Plantlets regenerated from callus were cultured on maintenance media and successfully transferred ex vitro. This study highlights the genotype-specific responses among rose cultivars and provides the first reports of in vitro regeneration for Italian Ice ® and Ringo All-Star™.