J. B. Rottman

author keywords: cytokines; mRNA expression; quantitative competitive polymerase chain reaction

MeSH headings : Animals; Base Sequence; Cats; Cattle; Cytokines / genetics; Cytokines / metabolism; Dogs; Gene Expression Regulation; Horses; Mammals; Molecular Sequence Data; Polymerase Chain Reaction / methods; Polymerase Chain Reaction / veterinary; RNA, Messenger / metabolism; RNA-Directed DNA Polymerase; Reproducibility of Results; Swine

TL;DR: Using consensus cytokine nucleic acid sequences, a previously described reverse transcription-quantitative competitive polymerase chain reaction technique is adapted to measure interleukin 2 (IL2), IL4, IL6, IL10, IL12, interferon γ, tumor necrosis factor α, and glyceraldehyde-3-phosphate dehydrogenase mRNA expression in the cow, cat, dog, horse, and pig. (via Semantic Scholar)

MeSH headings : Animals; Base Sequence; Blotting, Southern / veterinary; Cats / genetics; Cells, Cultured; Consensus Sequence; Cytokines / biosynthesis; Cytokines / chemistry; Cytokines / genetics; DNA Primers / chemistry; Electrophoresis, Agar Gel / veterinary; Gene Expression; Lymphocyte Activation; Lymphocytes / chemistry; Lymphocytes / metabolism; Male; Molecular Sequence Data; Oligonucleotide Probes / chemistry; Polymerase Chain Reaction / methods; Polymerase Chain Reaction / veterinary; RNA, Messenger / biosynthesis; RNA, Messenger / isolation & purification; Sensitivity and Specificity; Specific Pathogen-Free Organisms; Transcription, Genetic

TL;DR: The ability to detect feline cytokine expression would allow further characterization of the feline immune system and to detect the expression of other cytokines, a reverse transcription-polymerase chain reaction (RT-PCR) procedure was developed. (via Semantic Scholar)