2023 article

Blood Supplementation Enhances Bartonella henselae Growth and Molecular Detection of Bacterial DNA in Liquid Culture

Liedig, C., Neupane, P., Lashnits, E., Breitschwerdt, E. B., & Maggi, R. G. (2023, May 25). MICROBIOLOGY SPECTRUM.

By: C. Liedig n, P. Neupane *, E. Lashnits* , E. Breitschwerdt n  & R. Maggi n 

author keywords: BAPGM; Bartonella culture; Bartonella henselae; bartonellosis; blood supplementation; diagnostics
MeSH headings : Humans; Animals; Horses / genetics; Dogs; Sheep; Bartonella henselae / genetics; DNA, Bacterial / genetics; DNA, Bacterial / analysis; Bartonella Infections / diagnosis; Bartonella Infections / microbiology; Bartonella Infections / veterinary; Bartonella / genetics; Dietary Supplements; Mammals
TL;DR: The addition of sheep blood to liquid culture media increased the Bartonella henselae DNA concentration compared to controls, additionally improving PCR direct detection sensitivity. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: June 12, 2023

This study aims to improve diagnostic detection of Bartonella henselae . Patient samples are combined with enriched bacterial cultures aimed at growing Bartonella henselae for the best possible chance at detection. However, current Bartonella growth methods could be improved. The DNA extraction method used by most laboratories should also be optimized. Sheep blood was added to increase the growth of Bartonella henselae and multiple DNA extraction methods were to be compared to each other.