author keywords: catalysis; decarboxylation; enzymes; iron; reaction mechanisms

MeSH headings : Catalysis; Glycine / analogs & derivatives; Glycine / chemical synthesis; Hydroxylation; Iron / chemistry; Models, Molecular; Nitriles / chemical synthesis; Nuclear Magnetic Resonance, Biomolecular; Oxidation-Reduction; Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase / chemistry; X-Ray Diffraction

TL;DR: The plausible mechanism of three Fe/2OG enzymes, Sav607, ScoE and SfaA, catalyzed isonitrile formation is reported, and the X-ray structures of iron loaded ScoE in complex with its substrate and the intermediate, along with biochemical and biophysical data reveal that -N≡C bond formation involves two cycles of Fe/ 2OG enzyme catalysis. (via Semantic Scholar)