2021 journal article
Multiomic analysis defines the first microRNA atlas across all small intestinal epithelial lineages and reveals novel markers of almost all major cell types
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 321(6), G668–G681.
author keywords: intestinal epithelium; marker; microRNA; Paneth; tuft
MeSH headings : Animals; Biomarkers / metabolism; Cell Lineage; Cell Separation; Cells, Cultured; Computational Biology; Dogs; Epithelial Cells / metabolism; Female; Flow Cytometry; Gene Expression Profiling; Intestinal Mucosa / cytology; Intestinal Mucosa / metabolism; Intestine, Small / cytology; Intestine, Small / metabolism; Male; Mice, Inbred C57BL; Mice, Transgenic; MicroRNAs / genetics; MicroRNAs / metabolism; Organoids; RNA-Seq; Single-Cell Analysis; Transcriptome
TL;DR:
Comprehensive microRNA profiling in all major cell types of the mouse SI epithelium revealed highly enriched microRNA markers for almost every major cell type, and observed that the two most-enriched microRNAs in secretory progenitors are miR-1224 andMiR-672, the latter of which is deleted in hominin species.
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UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: January 3, 2022
In this study, first, microRNA atlas (and searchable web server) across all major small intestinal epithelial cell types is presented. We have demonstrated microRNAs that uniquely mark several lineages, including enteroendocrine and tuft. Identification of a key marker of mouse secretory progenitor cells, miR-672, which we show is deleted in humans. We have used several in vivo models to establish miR-152 as a specific marker of Paneth cells, which are highly understudied in terms of microRNAs.