2009 journal article
Molybdenum and tungsten in Campylobacter jejuni: their physiological role and identification of separate transporters regulated by a single ModE-like protein
MOLECULAR MICROBIOLOGY, 74(3), 758–771.
MeSH headings : ATP-Binding Cassette Transporters / genetics; ATP-Binding Cassette Transporters / metabolism; Amino Acid Sequence; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Campylobacter jejuni / genetics; Campylobacter jejuni / metabolism; Electron Transport / genetics; Escherichia coli / genetics; Escherichia coli / metabolism; Formate Dehydrogenases / genetics; Formate Dehydrogenases / metabolism; Humans; Membrane Transport Proteins / genetics; Membrane Transport Proteins / metabolism; Molecular Sequence Data; Molybdenum / chemistry; Molybdenum / metabolism; Nitrate Reductase / genetics; Nitrate Reductase / metabolism; Nitrate Reductase (NAD(P)H) / metabolism; Nitrate Reductases / chemistry; Nitrate Reductases / genetics; Nitrate Reductases / metabolism; Phylogeny; Protein Engineering; Pterins / metabolism; Sequence Alignment; Sequence Homology, Amino Acid; Transcription Factors / metabolism; Tungsten / chemistry; Tungsten / metabolism
TL;DR:
Results indicate that mod is primarily a molybdenum transporter that can also transport tungsten, while tup is a tungsen‐specific transporter.
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Added: August 6, 2018
Summary Campylobacter jejuni is an important human pathogen that causes millions of cases of food‐borne enteritis each year. The C. jejuni respiratory chain is highly branched and contains at least four enzymes predicted to contain a metal binding pterin (MPT), with the metal being either molybdenum or tungsten. Also predicted are two separate transport systems, one for molybdenum encoded by modABC and a second for tungsten encoded by tupABC. Both transport systems were mutated and the activities of the four predicted MPT‐containing enzymes were assayed in the presence of molybdenum and tungsten in wild‐type and mod and tup backgrounds. Results indicate that mod is primarily a molybdenum transporter that can also transport tungsten, while tup is a tungsten‐specific transporter. The MPT‐containing enzymes nitrate reductase, sulphite oxidase, and SN oxide reductase are strict molybdoenzymes while formate dehydrogenase prefers tungsten. A ModE‐like protein regulates both transporters, repressing mod in the presence of both molybdenum and tungsten and tup only in the presence of tungsten. Like other ModE proteins, the C. jejuni ModE binds DNA through a helix–turn–helix DNA binding domain, but unlike other members of the ModE family it does not have a metal binding domain.