Michael L. Sikes

Also known as: Michael L Sikes

Works (11)

Updated: February 22nd, 2026 12:10

2025 article

Knockdown of USF1 and USF2 drives prolonged changes in the gene expression response of M12-5B3 cells to DNA damage

Bellingham-Johnstun, K. S., Metzger, L. A., Stone, J. L., Gerrish, K., Bushel, P. R., & Sikes, M. L. (2025, July 14). PLoS ONE, Vol. 20.

By: K. Bellingham-Johnstun n, L. Metzger n, J. Stone  n, K. Gerrish, P. Bushel * & M. Sikes n 

MeSH headings : Animals; Mice; DNA Damage; Upstream Stimulatory Factors / genetics; Upstream Stimulatory Factors / metabolism; Cell Line, Tumor; Gene Knockdown Techniques; Tumor Suppressor Protein p53 / metabolism; Tumor Suppressor Protein p53 / genetics; Gene Expression Regulation, Neoplastic / radiation effects; Gene Expression Profiling
topics (OpenAlex): DNA Repair Mechanisms; PARP inhibition in cancer therapy; Immune Cell Function and Interaction
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3. Good Health and Well-being (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: September 8, 2025

2023 article

Pharmacogenomic Analyses Implicate B Cell Developmental Status and MKL1 as Determinants of Sensitivity toward Anti-CD20 Monoclonal Antibody Therapy

Small, G. W., Akhtari, F. S., Green, A. J., Havener, T. M., Sikes, M., Quintanhila, J., … Wiltshire, T. (2023, June 7). Cells, Vol. 12.

By: G. Small*, F. Akhtari *, A. Green n, T. Havener *, M. Sikes n , J. Quintanhila*, R. Gonzalez *, D. Reif*  ...

author keywords: rituximab (Rituxan (R)); ofatumumab (Arzerra (R),& nbsp;Kesimpta (R)); obinutuzumab (Gazyva (R)); CD20 (MS4A1); MKL1 (MRTFA); TGFB1
MeSH headings : Antibodies, Monoclonal / pharmacology; Antibodies, Monoclonal / therapeutic use; Antibodies, Monoclonal / genetics; Antigens, CD20 / genetics; Antineoplastic Agents; Pharmacogenomic Testing; Prednisolone; Humans
topics (OpenAlex): Chronic Lymphocytic Leukemia Research; Lymphoma Diagnosis and Treatment; Monoclonal and Polyclonal Antibodies Research
TL;DR: Results suggest that the efficacy of anti-CD20 mAb therapy may be influenced by B cell developmental status as well as polymorphism in the MKL1 gene. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, ORCID
Added: June 8, 2023

2022 journal article

Hands-on immunology: Engaging learners of all ages through tactile teaching tools

Frontiers in Microbiology, 13.

By: F. Harris  n, M. Sikes n , M. Bergman n, C. Goller n , A. Hasley n, C. Sjogren n, M. Ramirez n , C. Gordy n 

Contributors: M. Ramirez n 

author keywords: tactile teaching tool-guided inquiry learning; antibody; epitope; MHC; haplotype; universal design for learning; inclusive teaching; active learning
topics (OpenAlex): Innovative Teaching Methods; Problem and Project Based Learning; Experimental Learning in Engineering
TL;DR: Two TTT-GIL activities are reported here designed to engage diverse learners from middle schoolers to masters students in exploring molecular interactions within the immune system and can be adapted for use with learners of different levels and educational backgrounds. (via Semantic Scholar)
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3. Good Health and Well-being (OpenAlex)
4. Quality Education (Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: October 25, 2022

2011 article

The intestinal microbiota, gastrointestinal environment and colorectal cancer: a putative role for probiotics in prevention of colorectal cancer?

Azcárate-Peril, M. A., Sikes, M., & Bruno-Bárcena, J. M. (2011, June 24). American Journal of Physiology-Gastrointestinal and Liver Physiology, Vol. 301, pp. G401–G424.

By: M. Azcárate-Peril *, M. Sikes n  & J. Bruno-Bárcena n 

Contributors: M. Azcárate-Peril *, M. Sikes n  & J. Bruno-Bárcena n 

author keywords: lactic acid bacteria
MeSH headings : Antimutagenic Agents / pharmacology; Bile / physiology; Carcinogens; Cell Adhesion; Colorectal Neoplasms / genetics; Colorectal Neoplasms / microbiology; Colorectal Neoplasms / physiopathology; Colorectal Neoplasms / prevention & control; Diet; Gastrointestinal Tract / microbiology; Gastrointestinal Tract / physiology; Humans; Intestinal Mucosa / physiology; Intestines / microbiology; Metagenome; Oxidative Stress; Probiotics / therapeutic use
topics (OpenAlex): Gut microbiota and health; Probiotics and Fermented Foods; Digestive system and related health
TL;DR: Examination of probiotic metabolic activities that may have an effect on the prevention of CRC by scavenging toxic compounds or preventing their generation in situ are examined. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2010 article

Fur Negatively Regulates hns and Is Required for the Expression of HilA and Virulence in Salmonella enterica Serovar Typhimurium

Troxell, B., Sikes, M. L., Fink, R. C., Vazquez-Torres, A., Jones-Carson, J., & Hassan, H. M. (2010, November 13). Journal of Bacteriology, Vol. 193, pp. 497–505.

By: B. Troxell  n, M. Sikes n , R. Fink n, A. Vazquez-Torres *, J. Jones-Carson * & H. Hassan n 

MeSH headings : Animals; Bacterial Proteins / biosynthesis; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Chromatin Immunoprecipitation; DNA, Bacterial / metabolism; DNA-Binding Proteins / biosynthesis; DNA-Binding Proteins / metabolism; Disease Models, Animal; Gene Expression Regulation, Bacterial; Gene Knockout Techniques; Genes, Bacterial; Genomic Islands; Mice; Mice, Inbred C3H; Operon; Protein Binding; Repressor Proteins / genetics; Repressor Proteins / metabolism; Salmonella Infections, Animal / microbiology; Salmonella typhimurium / pathogenicity; Salmonella typhimurium / physiology; Survival Analysis; Trans-Activators / biosynthesis; Transcription Factors / biosynthesis; Virulence; Virulence Factors / biosynthesis
topics (OpenAlex): Salmonella and Campylobacter epidemiology; Bacteriophages and microbial interactions; Bacterial Genetics and Biotechnology
TL;DR: Fur is required for virulence in Salmonella enterica serovar Typhimurium and that Fur is necessary for the activation of hilA, as well as of other HilA-dependent genes, invF and sipC, and chromatin immunoprecipitation was used to show that Fur bound to the upstream region of hns in a metal-dependent fashion. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2010 article

Tetra-O-methyl nordihydroguaiaretic acid (Terameprocol) inhibits the NF-κB-dependent transcription of TNF-α and MCP-1/CCL2 genes by preventing RelA from binding its cognate sites on DNA

Oyegunwa, A. O., Sikes, M. L., Wilson, J. R., Scholle, F., & Laster, S. M. (2010, December 1). Journal of Inflammation, Vol. 7.

By: A. Oyegunwa n, M. Sikes n , J. Wilson  n, F. Scholle n  & S. Laster n

topics (OpenAlex): Immune Response and Inflammation; Natural product bioactivities and synthesis
TL;DR: TMP acts indirectly as an inhibitor of NF-κB-dependent transcription by preventing RelA from binding the promoters of certain key cytokine and chemokine genes. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2010 article

The Center of Accessibility: Dβ Control of V(D)J Recombination

Sikes, M. L., McMillan, R. E., & Bradshaw, J. M. (2010, October 1). Archivum Immunologiae Et Therapiae Experimentalis, Vol. 58, pp. 427–433.

By: M. Sikes n , R. McMillan n & J. Bradshaw n

author keywords: T cell receptor; Tcrb; Transcription; V(D)J recombination; Thymocytes; Double negative
MeSH headings : Acetylation; Animals; Chromatin Assembly and Disassembly; Epigenesis, Genetic; Gene Rearrangement, T-Lymphocyte; Genes, T-Cell Receptor beta; Histones / metabolism; Humans; Protein Processing, Post-Translational; Thymus Gland / enzymology; Thymus Gland / immunology; Transcription, Genetic; VDJ Recombinases / metabolism
topics (OpenAlex): T-cell and B-cell Immunology; Immune Cell Function and Interaction; Immunotherapy and Immune Responses
TL;DR: Examining Tcrb assembly in developing thymocytes, the central roles of RSS elements and germline promoters as foci for epigenetic reorganization of recombinationally accessible gene segments are reviewed in light of recent findings and persistent questions. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2009 article

A streamlined method for rapid and sensitive chromatin immunoprecipitation

Sikes, M. L., Bradshaw, J. M., Ivory, W. T., Lunsford, J. L., McMillan, R. E., & Morrison, C. R. (2009, March 27). Journal of Immunological Methods, Vol. 344, pp. 58–63.

By: M. Sikes n , J. Bradshaw n, W. Ivory n, J. Lunsford n, R. McMillan n & C. Morrison n

author keywords: Chromatin immunoprecipitation; Histone; Epigenetics; cad; TEA; Thymocyte; Rag
MeSH headings : Animals; Aspartate Carbamoyltransferase / genetics; Aspartate Carbamoyltransferase / metabolism; Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing) / genetics; Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing) / metabolism; Cell Line; Chromatin / genetics; Chromatin / isolation & purification; Chromatin / metabolism; Chromatin Immunoprecipitation / methods; DNA-Binding Proteins / genetics; DNA-Binding Proteins / metabolism; Dihydroorotase / genetics; Dihydroorotase / metabolism; Gene Knockdown Techniques; Histones / genetics; Histones / metabolism; Mice; Mice, Knockout; RNA Polymerase II / genetics; RNA Polymerase II / metabolism; T-Lymphocytes / metabolism
topics (OpenAlex): Genomics and Chromatin Dynamics; Epigenetics and DNA Methylation; RNA Interference and Gene Delivery
TL;DR: A streamlined ChIP is reported to efficiently carry samples from chromatin to qPCR-compatible DNA in as little as 4 h and it is shown that the protocol readily quantified histone modifications in chromatin from 10(4) Rag-deficient DN thymocytes. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2009 article

Molybdenum and tungsten in Campylobacter jejuni: their physiological role and identification of separate transporters regulated by a single ModE‐like protein

Taveirne, M. E., Sikes, M. L., & Olson, J. W. (2009, October 14). Molecular Microbiology, Vol. 74, pp. 758–771.

By: M. Taveirne n , M. Sikes n  & J. Olson* 

MeSH headings : ATP-Binding Cassette Transporters / genetics; ATP-Binding Cassette Transporters / metabolism; Amino Acid Sequence; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Campylobacter jejuni / genetics; Campylobacter jejuni / metabolism; Electron Transport / genetics; Escherichia coli / genetics; Escherichia coli / metabolism; Formate Dehydrogenases / genetics; Formate Dehydrogenases / metabolism; Humans; Membrane Transport Proteins / genetics; Membrane Transport Proteins / metabolism; Molecular Sequence Data; Molybdenum / chemistry; Molybdenum / metabolism; Nitrate Reductase / genetics; Nitrate Reductase / metabolism; Nitrate Reductase (NAD(P)H) / metabolism; Nitrate Reductases / chemistry; Nitrate Reductases / genetics; Nitrate Reductases / metabolism; Phylogeny; Protein Engineering; Pterins / metabolism; Sequence Alignment; Sequence Homology, Amino Acid; Transcription Factors / metabolism; Tungsten / chemistry; Tungsten / metabolism
topics (OpenAlex): Trace Elements in Health; Metal Extraction and Bioleaching; Corrosion Behavior and Inhibition
TL;DR: Results indicate that mod is primarily a molybdenum transporter that can also transport tungsten, while tup is a tungsen‐specific transporter. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2009 article

Promoter activity 5′ of Dβ2 is coordinated by E47, Runx1, and GATA-3

McMillan, R. E., & Sikes, M. L. (2009, July 10). Molecular Immunology, Vol. 46, pp. 3009–3017.

By: R. McMillan n & M. Sikes n 

author keywords: T cell receptor; Transcription; V(D)J recombination; Thymocytes; Double negative; E47; GATA-3; Runx1
MeSH headings : Animals; Base Sequence; Cell Differentiation / immunology; Cell Line; Chromatin / immunology; Chromatin / metabolism; Core Binding Factor Alpha 2 Subunit / immunology; Core Binding Factor Alpha 2 Subunit / metabolism; GATA3 Transcription Factor / immunology; GATA3 Transcription Factor / metabolism; Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; Homeodomain Proteins / genetics; Homeodomain Proteins / immunology; Homeodomain Proteins / metabolism; Mice; Mice, Knockout; Molecular Sequence Data; Promoter Regions, Genetic; Receptors, Antigen, T-Cell, alpha-beta / genetics; T-Lymphocytes / immunology; T-Lymphocytes / metabolism; TCF Transcription Factors / immunology; TCF Transcription Factors / metabolism; Thymus Gland / immunology; Thymus Gland / metabolism; Transcription Factor 7-Like 1 Protein; Tumor Suppressor Protein p53 / genetics; Tumor Suppressor Protein p53 / immunology; Tumor Suppressor Protein p53 / metabolism; Upstream Stimulatory Factors / immunology; Upstream Stimulatory Factors / metabolism
topics (OpenAlex): Ubiquitin and proteasome pathways; Cancer-related Molecular Pathways; Protein Kinase Regulation and GTPase Signaling
TL;DR: The dependence of 5'PDbeta2 on E47 is consistent with the reported role of E proteins in post-DN1 thymocyte development and V-to-DJbeta recombination and a complex interplay among Runx1, GATA-3, and E47 transcription factors. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2008 article

Differential Activation of Dual Promoters Alters Dβ2 Germline Transcription during Thymocyte Development

McMillan, R. E., & Sikes, M. L. (2008, March 1). The Journal of Immunology, Vol. 180, pp. 3218–3228.

By: R. McMillan n & M. Sikes n 

MeSH headings : Animals; Base Sequence; Cell Differentiation / genetics; Cell Differentiation / immunology; Cell Line; Cell Line, Tumor; Gene Rearrangement, beta-Chain T-Cell Antigen Receptor / genetics; Germ-Line Mutation / immunology; Mice; Mice, Inbred C57BL; Mice, Knockout; Molecular Sequence Data; Promoter Regions, Genetic / immunology; Receptors, Antigen, T-Cell, alpha-beta / genetics; Receptors, Antigen, T-Cell, alpha-beta / metabolism; Thymus Gland / cytology; Thymus Gland / enzymology; Thymus Gland / immunology; Thymus Gland / metabolism; Transcription, Genetic / immunology; Transcriptional Activation / immunology; VDJ Recombinases / genetics
topics (OpenAlex): T-cell and B-cell Immunology; Immune Cell Function and Interaction
TL;DR: The unique organization and timing of Dβ2 promoter activity are consistent with a model in which promoter placement selectively regulates the rearrangement potential of D β2 during TCR β locus assembly. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

Employment

Updated: January 28th, 2026 11:13

2003 - present

North Carolina State University Raleigh, NC, US
Associate Professor Biological Sciences

1999 - 2002

Vanderbilt University Nashville, Tennessee, US
Instructor Microbiology and Immunology

Education

Updated: January 28th, 2026 11:13

1995 - 1999

Vanderbilt University Nashville, Tennessee, US
Postdoctoral Fellow Microbiology and Immunology

1990 - 1995

Baylor College of Medicine Houston, Texas, US
PhD Cell Biology

1986 - 1990

University of Arkansas at Little Rock Little Rock, Arkansas, US
BS Biology

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