Nicholas J. Watkins

Works (3)

Updated: August 16th, 2024 13:37

1998 journal article

In vitro assembly of the mouse U14 snoRNP core complex and identification of a 65-kDa box C/D-binding protein

RNA, 4(5), 582–593.

By: N. Watkins n, D. Newman n, J. Kuhn n & E. Maxwell n

author keywords: nucleolus; RNA processing, RNA-protein interaction; snoRNA
MeSH headings : Animals; Ascitic Fluid / cytology; Cell Extracts; Cross-Linking Reagents; HeLa Cells; Humans; Mice; Molecular Weight; Nucleic Acid Conformation; Oocytes; Protein Binding; RNA, Small Nuclear / chemistry; RNA, Small Nuclear / genetics; RNA, Small Nuclear / metabolism; RNA-Binding Proteins / chemistry; RNA-Binding Proteins / isolation & purification; RNA-Binding Proteins / metabolism; Ribonucleoproteins, Small Nuclear / biosynthesis; Ribonucleoproteins, Small Nuclear / chemistry; Ultraviolet Rays; Xenopus
TL;DR: An in vitro U14 snoRNP assembly system is established and electrophoretic mobility-shift analysis demonstrated that all the sequences and structures of the box C/D core motif required for U14 processing are also necessary for protein binding and sno RNP assembly. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

1998 journal article

Processing of vertebrate box C/D small nucleolar RNAs in plant cells

EUROPEAN JOURNAL OF BIOCHEMISTRY, 253(1), 154–160.

By: D. Leader, G. Clark, J. Boag, J. Watters, C. Simpson, N. Watkins*, E. Maxwell n, J. Brown

author keywords: small nucleolar RNA; box C/D small nucleolar RNA; small nucleolar RNA processing; U14
MeSH headings : Animals; Base Sequence; Female; Humans; In Vitro Techniques; Introns; Mice; Oligodeoxyribonucleotides / genetics; Oocytes / metabolism; Plants, Genetically Modified; Plants, Toxic; RNA Processing, Post-Transcriptional; RNA Splicing; RNA, Small Nuclear / chemistry; RNA, Small Nuclear / genetics; RNA, Small Nuclear / metabolism; Tobacco / genetics; Tobacco / metabolism; Xenopus
TL;DR: The similarity of processing behaviour of the vertebrate box C/D constructs in tobacco protoplasts and Xenopus oocytes suggests the mechanism of processing, involving recognition and association of proteins, is conserved in plants. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

1997 journal article

Identification of specific nucleotide sequences and structural elements required for intronic U14 snorna processing

RNA, 3(1), 17–26.

By: L. Xia, N. Watkins & E. Maxwell

Source: NC State University Libraries
Added: August 6, 2018

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