Works (3)
Updated: July 5th, 2023 16:04
1998 article
Cloning and expression of a streptavidin-lipase fusion gene in Escherichia coli and characterization of the immobilized fusion protein
Lee, P., & Swaisgood, H. E. (1998, March 1). Enzyme and Microbial Technology.
author keywords: streptavidin-lipase fusion gene; fusion protein; Escherichia coli; cloning; transesterification
topics (OpenAlex): Biotin and Related Studies; Enzyme Catalysis and Immobilization; Bacterial Genetics and Biotechnology
TL;DR:
Streptavidin-lipase fusion protein was purified and immobilized in a single step from recombinant cell lysates by bioselective adsorption on 6-(biotinamido)hexanamidopropyl-controlled pore glass and exhibited a pH optimum between 7 and 8 and a preference for short-chain fatty acids.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
1997 article
Characterization of a Chemically Conjugated Lipase Bioreactor
Lee, P., & Swaisgood, H. E. (1997, August 1). Journal of Agricultural and Food Chemistry.
author keywords: biotinylated lipase; immobilized lipase; immobilized avidin; interesterification
topics (OpenAlex): Enzyme Catalysis and Immobilization; Analytical Chemistry and Chromatography; Electrochemical sensors and biosensors
TL;DR:
The hydrolytic specific activity of lipase immobilization on CPG-3000 and on nonporous beads was essentially the same as that for the biotinylated free enzyme, whereas that immobilized on C PG-2000 was about 50% less.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
12. Responsible Consumption and Production
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
1997 article
Modification of Milkfat Physical Properties by ImmobilizedPseudomonas fluorescensLipase
Lee, P., & Swaisgood, H. E. (1997, August 1). Journal of Agricultural and Food Chemistry.
author keywords: milkfat; immobilized lipase; streptavidin-lipase; fusion protein; interesterification
topics (OpenAlex): Enzyme Catalysis and Immobilization; Food Chemistry and Fat Analysis; Microbial Metabolic Engineering and Bioproduction
TL;DR:
A bifunctional fusion protein was constructed by fusion of the streptavidin gene from Streptomyces avidinii to the lipase gene from Pseudomonas fluorescens, and the resulting strePTavidin−lipase was expressed in Escherichia coli.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
12. Responsible Consumption and Production
(Web of Science)
Source: Web Of Science
Added: August 6, 2018