Peychii Lee

Works (3)

Updated: July 5th, 2023 16:04

1998 journal article

Cloning and expression of a streptavidin-lipase fusion gene in Escherichia coli and characterization of the immobilized fusion protein

ENZYME AND MICROBIAL TECHNOLOGY, 22(4), 246–254.

By: P. Lee n & H. Swaisgood n

author keywords: streptavidin-lipase fusion gene; fusion protein; Escherichia coli; cloning; transesterification
TL;DR: Streptavidin-lipase fusion protein was purified and immobilized in a single step from recombinant cell lysates by bioselective adsorption on 6-(biotinamido)hexanamidopropyl-controlled pore glass and exhibited a pH optimum between 7 and 8 and a preference for short-chain fatty acids. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

1997 journal article

Characterization of a chemically conjugated lipase bioreactor

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 45(8), 3350–3356.

By: P. Lee n & H. Swaisgood n

author keywords: biotinylated lipase; immobilized lipase; immobilized avidin; interesterification
TL;DR: The hydrolytic specific activity of lipase immobilization on CPG-3000 and on nonporous beads was essentially the same as that for the biotinylated free enzyme, whereas that immobilized on C PG-2000 was about 50% less. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

1997 journal article

Modification of milkfat physical properties by immobilized Pseudomonas fluorescens lipase

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 45(8), 3343–3349.

By: P. Lee n & H. Swaisgood n

author keywords: milkfat; immobilized lipase; streptavidin-lipase; fusion protein; interesterification
TL;DR: A bifunctional fusion protein was constructed by fusion of the streptavidin gene from Streptomyces avidinii to the lipase gene from Pseudomonas fluorescens, and the resulting strePTavidin−lipase was expressed in Escherichia coli. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

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