Harold E Swaisgood

Works (47)

Updated: August 16th, 2024 13:38

2007 journal article

Trypsin imobilization on derivatized cellulose beads by biospecific avidin-biotin interaction and characterization of the immobilized activity

Journal of Food Biochemistry, 26(2), 119–129.

By: V. Janolino n & H. Swaisgood n

TL;DR: It is indicated that cellulose beads can be biotinylated for use as bioselective support for trypsin bioreactors and avidin-biotin interaction. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Source: NC State University Libraries
Added: August 6, 2018

2004 journal article

Cross-linking and rheological changes of whey proteins treated with microbial transglutaminase

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 52(5), 1170–1176.

By: V. Truong n, D. Clare, G. Catignani n & H. Swaisgood n

author keywords: whey protein; immobilized transglutaminase; transglutaminase; protein cross-linking; electrophoresis; Western blots; rheology
MeSH headings : Adsorption; Bacteria / enzymology; Cross-Linking Reagents; Enzymes, Immobilized; Milk Proteins / chemistry; Milk Proteins / metabolism; Rheology; Transglutaminases / metabolism; Whey Proteins
TL;DR: Interestingly, the gelling point of WPI solutions increased from 68 to 94 degrees C after a 4-h reaction, and the gel strength was drastically decreased, suggesting that a process could be developed to produce heat-stable whey proteins for various food applications. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2004 review

Nomenclature of the proteins of cows' milk - Sixth revision

[Review of ]. JOURNAL OF DAIRY SCIENCE, 87(6), 1641–1674.

By: H. Farrell*, R. Jimenez-Flores *, G. Bleck, E. Brown *, J. Butler *, L. Creamer*, C. Hicks *, C. Hollar, K. Ng-Kwai-Hang*, H. Swaisgood n

author keywords: milk protein; structure; nomenclature; review
MeSH headings : Amino Acid Sequence; Animals; Caseins / chemistry; Caseins / classification; Cattle; Immunoglobulins / chemistry; Immunoglobulins / classification; Lactoglobulins; Milk Proteins / chemistry; Milk Proteins / classification; Molecular Sequence Data; Molecular Weight; Sequence Analysis, Protein; Sequence Homology, Amino Acid; Terminology as Topic; Whey Proteins
TL;DR: Significant findings related to nomenclature and protein methodology are elucidation of several new genetic variants of the major milk proteins, establishment by sequencing techniques and sequence alignment of the bovine caseins and whey proteins as the reference point for the nomenClature of all homologous milk proteins. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2004 personal communication

Rebuttal on a semi-pilot-scale procedure for isolating and purifying soybean (Glycine max) lectin

Fasina, Y. O., Swaisgood, H. E., Garlich, J. D., & Classen, H. L. (2004, May 19).

By: Y. Fasina  n, H. Swaisgood n, J. Garlich n & H. Classen n

UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2004 review

The importance of disulfide bridging

[Review of ]. BIOTECHNOLOGY ADVANCES, 23(1), 71–73.

By: H. Swaisgood n

MeSH headings : Disulfides / chemistry; Disulfides / metabolism; Food Handling; Lactoglobulins / chemistry; Lactoglobulins / metabolism; Protein Disulfide-Isomerases / chemistry; Protein Disulfide-Isomerases / metabolism; Proteins / chemistry; Proteins / metabolism; Sulfhydryl Compounds / chemistry; Sulfhydryl Compounds / metabolism
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2003 journal article

A semi-pilot-scale procedure for isolating and purifying soybean (Glycine max) lectin

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 51(16), 4532–4538.

By: Y. Fasina  n, H. Swaisgood n, J. Garlich n & H. Classen n

author keywords: soybean lectin (SBL); Glycine max; lectin purification; scale-up; affinity chromatography; SDS- and native-PAGE
MeSH headings : Chromatography, Affinity; Electrophoresis, Polyacrylamide Gel; Pilot Projects; Plant Lectins / isolation & purification; Soybean Proteins / isolation & purification; Soybeans / chemistry; Ultrafiltration
TL;DR: Electrophoretic analysis and hemagglutination assay revealed that the freeze-dried SBL was similar to Sigma-grade SBL in purity and activity (35 and 33 HU/mg protein, respectively). (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2003 review

Biodefense properties of milk: The role of antimicrobial proteins and peptides

[Review of ]. CURRENT PHARMACEUTICAL DESIGN, 9(16), 1239–1255.

By: D. Clare n, G. Catignani* & H. Swaisgood*

author keywords: biodefense proteins and peptides; antimicrobial milk proteins; antimicrobial milk peptides; immunoregulatory milk proteins; immunoregulatory milk peptides; review article
MeSH headings : Adjuvants, Immunologic / chemistry; Adjuvants, Immunologic / pharmacology; Animals; Anti-Bacterial Agents / chemistry; Anti-Bacterial Agents / pharmacology; Colostrum / chemistry; Colostrum / immunology; Humans; Milk / chemistry; Milk / immunology; Milk Proteins / chemistry; Milk Proteins / immunology; Milk Proteins / pharmacology; Milk, Human / chemistry; Milk, Human / immunology; Peptides
TL;DR: The principal biological properties associated with each of these contributing milk components are reviewed with a special emphasis on the role of biodefensive milk peptides. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2003 journal article

Investigating the possibility of monitoring lectin levels in commercial soybean meals intended for poultry feeding using steam-heated soybean meal as a model

POULTRY SCIENCE, 82(4), 648–656.

By: Y. Fasina*, H. Classen*, J. Garlich*, H. Swaisgood* & D. Clare*

author keywords: soybean meal; lectin; urease activity; trypsin inhibitor; protein solubility
MeSH headings : Animal Feed / analysis; Animal Nutritional Physiological Phenomena; Animals; Food Handling / methods; Hydrogen-Ion Concentration; Nutritive Value; Plant Lectins / analysis; Plant Lectins / metabolism; Poultry / metabolism; Soybean Proteins / analysis; Soybean Proteins / metabolism; Temperature; Time Factors; Trypsin Inhibitors / analysis; Urease / metabolism
TL;DR: The denaturation pattern of UA was highly correlated with that of SBL, indicating that UA could be used for monitoring lectin levels in commercial meals, and it was concluded that UA of 0.03 to 0.09 units of pH change are indicative of adequately processed meals that contain negligible Lectin levels. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2003 journal article

Production and characterization of bio-immobilized keratinase in proteolysis and keratinolysis

ENZYME AND MICROBIAL TECHNOLOGY, 32(7), 812–819.

By: J. Wang n, H. Swaisgood n & J. Shih  n

author keywords: keratinase; bio-immobililzation; streptavidin; fusion protein
TL;DR: Extracellular production of keratinase–streptavidin fusion protein was accomplished by the cloning of Bacillus subtilis with a transforming plasmid carrying the kerA - stp fusion gene. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2002 journal article

Bioimmobilization of keratinase using Bacillus subtilis and Escherichia coli systems

BIOTECHNOLOGY AND BIOENGINEERING, 81(4), 421–429.

By: J. Wang n, H. Swaisgood n & J. Shih  n

author keywords: keratinase; immobilization; streptavidin; fusion protein
MeSH headings : Bacillus subtilis / classification; Bacillus subtilis / enzymology; Bacillus subtilis / genetics; Cells, Cultured; Enzymes, Immobilized / chemical synthesis; Enzymes, Immobilized / genetics; Enzymes, Immobilized / isolation & purification; Enzymes, Immobilized / metabolism; Escherichia coli / classification; Escherichia coli / enzymology; Gene Expression Regulation, Bacterial; Gene Expression Regulation, Enzymologic; Macromolecular Substances; Peptide Hydrolases / classification; Peptide Hydrolases / genetics; Peptide Hydrolases / isolation & purification; Peptide Hydrolases / metabolism; Protein Binding; Protein Engineering / methods; Recombinant Fusion Proteins / biosynthesis; Recombinant Fusion Proteins / genetics; Species Specificity; Streptavidin / biosynthesis; Streptavidin / classification; Streptavidin / genetics; Streptavidin / isolation & purification
TL;DR: A new genetic construct was developed to produce the keratinase-streptavidin fusion protein, so that the purification and immobilization of the fusion protein onto a biotinylated matrix can be accomplished in a single step. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2002 journal article

Immobilization and utilization of the recombinant fusion proteins trypsin-streptavidin and streptavidin-transglutaminase for modification of whey protein isolate functionality

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 50(13), 3723–3730.

By: C. Wilcox n, D. Clare n, V. Valentine n & H. Swaisgood n

author keywords: immobilization; trypsin; transglutaminase; whey protein; functionality
MeSH headings : Adsorption; Biotinylation; Chemical Phenomena; Chemistry, Physical; Cross-Linking Reagents; DNA, Recombinant; Dithiothreitol / pharmacology; Enzymes, Immobilized; Escherichia coli / genetics; Gels; Glass; Milk Proteins / chemistry; Milk Proteins / metabolism; Recombinant Fusion Proteins; Streptavidin / genetics; Temperature; Transglutaminases / genetics; Transglutaminases / metabolism; Trypsin / genetics; Trypsin / metabolism; Viscosity; Whey Proteins
TL;DR: By combining limited proteolysis with controlled cross-linking, it was possible to create derivatives of whey proteins with enhanced functional properties that allowed for recycling of the enzyme, eliminated the requirement for a downstream inactivation step, and permitted control over the extent of modification. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2002 journal article

Isolation and partial characterization of CD36 from skim milk

JOURNAL OF DAIRY SCIENCE, 85(8), 1903–1908.

By: C. Wilcox n, V. Janolino n & H. Swaisgood n

author keywords: CD36; milk fat globule membrane; skim milk; sulfhydryl oxidase
MeSH headings : Amino Acid Sequence; Animals; Blotting, Western; CD36 Antigens; Cattle; Centrifugation; Chromatography, Gel; Electrophoresis, Polyacrylamide Gel; Glycolipids / analysis; Glycoproteins / analysis; Glycosylation; Lipid Droplets; Membrane Glycoproteins / chemistry; Membrane Glycoproteins / isolation & purification; Milk / chemistry; Molecular Sequence Data; Molecular Weight; Organic Anion Transporters / chemistry; Organic Anion Transporters / isolation & purification; Ultrafiltration
TL;DR: CD36, a common milk fat globule membrane glycoprotein, was isolated from skim milk by methods similar to those previously utilized for the isolation of sulfhydryl oxidase, giving similar purity as observed by electrophoresis and suggested tissue-specific glycosylation. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2002 journal article

Modification of the rheological properties of whey protein isolate through the use of an immobilized microbial transglutaminase

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 50(20), 5546–5551.

By: C. Wilcox n & H. Swaisgood n

author keywords: immobilization; transglutaminase; viscosity; whey protein isolate; gelation; cross linking
MeSH headings : Adsorption; Avidin / chemistry; Bacteria / enzymology; Biotinylation; Cross-Linking Reagents; Enzymes, Immobilized; Glass; Microspheres; Milk Proteins / chemistry; Rheology; Solutions; Transglutaminases / metabolism; Viscosity; Whey Proteins
TL;DR: The functional properties of several batches of WPI were modified using <10 mg of the same enzyme, illustrating the capacity of immobilized enzymes to be used more frequently in applications of this nature. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2002 journal article

Purification and characterization of the fusion protein trypsin-streptavidin expressed in Escherichia coli

JOURNAL OF PROTEIN CHEMISTRY, 21(6), 413–418.

By: F. Zhao n, D. Clare n, G. Catignani n & H. Swaisgood n

author keywords: affinity column; fusion protein; protein expression; protein purification; trypsin; streptavidin
MeSH headings : Blotting, Western; Chromatography, Affinity; Electrophoresis, Polyacrylamide Gel; Escherichia coli / genetics; Recombinant Fusion Proteins / chemistry; Recombinant Fusion Proteins / genetics; Recombinant Fusion Proteins / isolation & purification; Streptavidin / chemistry; Trypsin / chemistry
TL;DR: The molecular size of the soluble purified fusion protein was determined by size-exclusion chromatography using Superose 12 FPLC, indicating that the soluble protein exists as a monomer; thus, the presence of the trypsin domain must prevent the streptavidin domain from tetramer formation. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2001 journal article

Gelation properties of dispersions containing polymerized and native whey protein isolate

FOOD HYDROCOLLOIDS, 15(2), 165–175.

By: B. Vardhanabhuti n, E. Foegeding n, M. McGuffey n, C. Daubert n & H. Swaisgood n

author keywords: whey protein isolate; gelation; rheological properties
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2001 journal article

Identification of peptide ligands generated by combinatorial chemistry that bind alpha-lactalbumin

SEPARATION SCIENCE AND TECHNOLOGY, 36(11), 2411–2431.

By: P. Gurgel n, R. Carbonell n & H. Swaisgood n

TL;DR: The identification of the hexapeptide WHWRKR, obtained from a combinatorial library, that shows affinity for α-lactalbumin is described. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2001 journal article

Molecular design, expression, and affinity immobilization of a trypsin-streptavidin fusion protein

ENZYME AND MICROBIAL TECHNOLOGY, 28(6), 483–491.

By: D. Clare n, V. Valentine n, G. Catignani n & H. Swaisgood n

author keywords: cloning; protein expression; immobilized enzymes; trypsin
TL;DR: This enzyme bioreactor should serve as an excellent prototype for future studies that will examine the effect of limited proteolysis on functional characteristics of milk proteins, including gelling, emulsifying and foaming properties. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2001 patent

Protein ingredient for carrying lipophilic nutrients

Washington, DC: U.S. Patent and Trademark Office.

By: H. Swaisgood, Q. Wang & J. Allen

Source: NC State University Libraries
Added: August 6, 2018

2001 journal article

Studies of the binding of alpha-lactalbumin to immobilized peptide ligands

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 49(12), 5765–5770.

By: P. Gurgel n, R. Carbonell n & H. Swaisgood n

author keywords: alpha-lactalbumin; peptide ligands; bioselective adsorption; binding mechanism
MeSH headings : Adsorption; Amino Acid Sequence; Lactalbumin / metabolism; Ligands; Mutagenesis, Site-Directed; Oligopeptides / metabolism; Protein Binding; Protein Precursors / metabolism; Recombinant Proteins / metabolism; Resins, Plant; Thermodynamics
TL;DR: The present work investigates the mechanism of binding of α-lactalbumin to the peptide ligand WH WRKR and its variants HWRKR and acetylated WHWRKR immobilized on a polymethacrylate chromatographic resin. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2000 review

Bioactive milk peptides: A prospectus

[Review of ]. JOURNAL OF DAIRY SCIENCE, 83(6), 1187–1195.

By: D. Clare n & H. Swaisgood n

author keywords: bioactive peptide; milk proteins; functional foods
MeSH headings : Angiotensin-Converting Enzyme Inhibitors / chemistry; Angiotensin-Converting Enzyme Inhibitors / metabolism; Animals; Cattle; Fibrinolytic Agents / chemistry; Fibrinolytic Agents / metabolism; Milk / microbiology; Milk / physiology; Milk Proteins / chemistry; Milk Proteins / pharmacology; Milk Proteins / therapeutic use; Opioid Peptides / chemistry; Opioid Peptides / physiology; Peptides / analysis; Peptides / physiology
TL;DR: This work reviews the scientific literature and attempts to stimulate consideration of the continued use of bioactive peptides and their expanded development as a commercial product. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2000 journal article

Fractionation of whey proteins with a hexapeptide ligand affinity resin

BIOSEPARATION, 9(6), 385–392.

By: P. Gurgel n, R. Carbonell n & H. Swaisgood n

author keywords: alpha-lactalbumin; bioselective adsorption; peptide ligands; whey protein isolate
MeSH headings : Animals; Cattle; Chromatography, Affinity / methods; Lactalbumin / isolation & purification; Ligands; Milk Proteins / isolation & purification; Oligopeptides; Resins, Synthetic; Whey Proteins
TL;DR: This study describes two methods for production of α-lactalbumin from whey protein isolate using bioselective adsorption and the combined use of an amino resin followed by the WHWRKR resin produce a highly purified α- lact Albumin (100%). (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2000 journal article

Thermodynamics of unfolding of beta-trypsin at pH 2.8

Archives of Biochemistry and Biophysics, 382(1), 57–62.

By: M. Brumano *, E. Rogana* & H. Swaisgood n

MeSH headings : Animals; Calorimetry; Cattle; Circular Dichroism; Hydrogen-Ion Concentration; Protein Folding; Spectrometry, Fluorescence; Spectrophotometry; Temperature; Thermodynamics; Trypsin / chemistry; Trypsin / metabolism; Ultraviolet Rays; Urea / pharmacology
TL;DR: The value for the stability of β-trypsin estimated from spectroscopic data is in reasonable agreement with the value calculated from urea titration curves, which supports the two-state model for the unfolding of this protein. (via Semantic Scholar)
Source: NC State University Libraries
Added: August 6, 2018

1999 journal article

Binding of lipophilic nutrients to beta-lactoglobulin prepared by bioselective adsorption

JOURNAL OF DAIRY SCIENCE, 82(2), 257–264.

By: Q. Wang n, J. Allen* & H. Swaisgood*

author keywords: binding lipophilic nutrients; beta-lactoglobulin; bioselective adsorption
MeSH headings : Adsorption; Binding, Competitive; Diterpenes; Ergocalciferols / metabolism; Hydrogen-Ion Concentration; Lactoglobulins / metabolism; Retinaldehyde / metabolism; Retinyl Esters; Spectrometry, Fluorescence; Tryptophan / chemistry; Vitamin A / analogs & derivatives; Vitamin A / metabolism
TL;DR: The results are consistent with the model in which the retinoids and vitamin D2 bind in the calyx formed by the beta-barrel; palmitate and a second molecule of vitamin D 2 bind in a surface pocket near the dimer contact region. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

1999 journal article

Modification of rheological properties of whey protein isolates by limited proteolysis

NAHRUNG-FOOD, 43(2), 79–85.

By: X. Huang n, G. Catignani n & H. Swaisgood*

UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

1998 journal article

Calcium binding of phosphopeptides derived from hydrolysis of alpha(s)-Casein or beta-Casein using immobilized trypsin

JOURNAL OF DAIRY SCIENCE, 81(11), 2850–2857.

By: O. Park n, H. Swaisgood n & J. Allen n

author keywords: casein; immobilized trypsin; casein phosphopeptide; calcium binding
MeSH headings : Amino Acid Sequence; Animals; Binding Sites; Calcium / metabolism; Caseins / chemistry; Caseins / metabolism; Enzymes, Immobilized; Hydrolysis; Milk / chemistry; Molecular Sequence Data; Peptide Fragments / metabolism; Phosphopeptides / metabolism; Trypsin / metabolism
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

1998 journal article

Cloning and expression of a streptavidin-lipase fusion gene in Escherichia coli and characterization of the immobilized fusion protein

ENZYME AND MICROBIAL TECHNOLOGY, 22(4), 246–254.

By: P. Lee n & H. Swaisgood n

author keywords: streptavidin-lipase fusion gene; fusion protein; Escherichia coli; cloning; transesterification
TL;DR: Streptavidin-lipase fusion protein was purified and immobilized in a single step from recombinant cell lysates by bioselective adsorption on 6-(biotinamido)hexanamidopropyl-controlled pore glass and exhibited a pH optimum between 7 and 8 and a preference for short-chain fatty acids. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

1998 journal article

Construction and expression of a bifunctional single-chain antibody against Bacillus cereus spores

Applied and Environmental Microbiology, 64(7), 2490–2496.

By: K. Koo, P. Foegeding & H. Swaisgood

Source: NC State University Libraries
Added: August 6, 2018

1998 journal article

Development of a streptavidin-conjugated single-chain antibody that binds Bacillus cereus spores

Applied and Environmental Microbiology, 64(7), 2497–2502.

By: K. Koo, P. Foegeding & H. Swaisgood

Source: NC State University Libraries
Added: August 6, 2018

1998 journal article

Isolation of RNA and DNA fragments using diatomaceous earth

BIOTECHNOLOGY TECHNIQUES, 12(7), 549–552.

By: K. Koo, P. Foegeding* & H. Swaisgood*

TL;DR: A series of simple and phenol-free silica-based protocols for isolating and cleaning RNA or DNA fragments from different sources were developed and Cytoplasmic RNA isolated from hybridoma cells by this method was used in RT-PCR. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

1998 journal article

Protein concentration dependence of palmitate binding to beta-lactoglobulin

JOURNAL OF DAIRY SCIENCE, 81(1), 76–81.

By: Q. Wang n, J. Allen n & H. Swaisgood n

author keywords: beta-lactoglobulin; palmitate; palmitate binding
TL;DR: Formation of the dimer, by hydrophobic interactions at the monomers contact site, eliminated palmitate binding sites on the monomer but formed a higher affinity pocket for binding to theDimer. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

1997 journal article

Binding of retinoids to beta-lactoglobulin isolated by bioselective adsorption

JOURNAL OF DAIRY SCIENCE, 80(6), 1047–1053.

By: Q. Wang n, J. Allen n & H. Swaisgood n

author keywords: beta-lactoglobulin; retinoid binding; bioselective adsorption; whey protein isolate
MeSH headings : Adsorption; Animals; Cattle; Chromatography, Gel / methods; Chromatography, Gel / veterinary; Female; Hydrogen-Ion Concentration; Lactoglobulins / analysis; Lactoglobulins / chemistry; Lactoglobulins / metabolism; Milk / chemistry; Milk Proteins / analysis; Protein Binding; Retinoids / chemistry; Retinoids / metabolism; Spectrometry, Fluorescence / methods; Spectrometry, Fluorescence / veterinary; Whey Proteins
TL;DR: Binding of β-lactoglobulin (LG) that had been prepared by bioselective adsorption on N-retinyl-Celite TM was determined from changes in the fluorescence quenching of the protein tryptophanyl residues, suggesting that a tryptophile is in the binding site. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

1997 journal article

Binding of vitamin D and cholesterol to beta-lactoglobulin

JOURNAL OF DAIRY SCIENCE, 80(6), 1054–1059.

By: Q. Wang n, J. Allen* & H. Swaisgood*

author keywords: beta-lactoglobulin; vitamin D binding; cholesterol binding; bioselective adsorption
MeSH headings : Adsorption; Animals; Cattle; Cholecalciferol / chemistry; Cholecalciferol / metabolism; Cholesterol / chemistry; Cholesterol / metabolism; Chromatography, Gel / methods; Chromatography, Gel / veterinary; Dehydrocholesterols / metabolism; Ergocalciferols / chemistry; Ergocalciferols / metabolism; Ergosterol / chemistry; Ergosterol / metabolism; Female; Lactoglobulins / chemistry; Lactoglobulins / metabolism; Protein Binding; Retinoids / chemistry; Retinoids / metabolism; Spectrometry, Fluorescence / methods; Spectrometry, Fluorescence / veterinary; Vitamin D / chemistry; Vitamin D / metabolism
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

1997 journal article

Characterization of a chemically conjugated lipase bioreactor

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 45(8), 3350–3356.

By: P. Lee n & H. Swaisgood n

author keywords: biotinylated lipase; immobilized lipase; immobilized avidin; interesterification
TL;DR: The hydrolytic specific activity of lipase immobilization on CPG-3000 and on nonporous beads was essentially the same as that for the biotinylated free enzyme, whereas that immobilized on C PG-2000 was about 50% less. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

1997 journal article

Comparison of the properties of trypsin immobilized on 2 Celite(TM) derivatives

JOURNAL OF BIOTECHNOLOGY, 53(1), 21–27.

By: X. Huang n, G. Catignani n & H. Swaisgood n

author keywords: immobilized trypsin; succinamidopropyl-Celite; aminopropyl-Celite; beta-lactoglobulin
MeSH headings : Diatomaceous Earth / chemistry; Enzymes, Immobilized / chemistry; Enzymes, Immobilized / metabolism; Hydrogen-Ion Concentration; Kinetics; Trypsin / chemistry; Trypsin / metabolism
TL;DR: The two methods of immobilization on Celite gave biocataysts with similar kinetic properties but immobilized on SAPC yielded slightly higher loadings and higher specific activities. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

1997 journal article

Development and characterization of a bioselective adsorption matrix for removal of Bacillus cereus spores from buffer and milk

Food Science & Technology = Lebensmittel-Wissenschaft & -Technologie, 30(8), 786–792.

By: D. Darquea n, H. Swaisgood n & P. Foegeding n

Source: NC State University Libraries
Added: August 6, 2018

1997 journal article

Micro-scale method for determining foaming properties of protein

JOURNAL OF FOOD SCIENCE, 62(5), 1028-&.

By: X. Huang n, G. Catignani n & H. Swaisgood n

author keywords: microscale whipping method; foam properties; protein
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

1997 journal article

Modification of milkfat physical properties by immobilized Pseudomonas fluorescens lipase

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 45(8), 3343–3349.

By: P. Lee n & H. Swaisgood n

author keywords: milkfat; immobilized lipase; streptavidin-lipase; fusion protein; interesterification
TL;DR: A bifunctional fusion protein was constructed by fusion of the streptavidin gene from Streptomyces avidinii to the lipase gene from Pseudomonas fluorescens, and the resulting strePTavidin−lipase was expressed in Escherichia coli. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

1997 journal article

Production and purification of an active bovine lysozyme in tobacco (Nicotiana tabacum): Utilization of value-added crop plants traditionally grown under intensive agriculture

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 45(7), 2793–2797.

By: C. Wilcox n, A. Weissinger n, R. Long n, L. Fitzmaurice n, T. Mirkov n & H. Swaisgood n

author keywords: transgenic; lysozyme; recombinant protein; antimicrobial; Nicotiana tabacum
TL;DR: It was concluded that tobacco was an excellent choice for expression of the recombinant protein and that the purification process developed in this study demonstrates methodology for isolation of high-value enzymes from tobacco and other crop plants. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

1997 journal article

Purity and yield of beta-lactoglobulin isolated by an N-retinyl-Celite bioaffinity column

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 45(7), 2369–2373.

By: R. Heddleson n, J. Allen n, Q. Wang n & H. Swaisgood n

author keywords: beta-lactoglobulin; alpha-lactalbumin; bioselective adsorption; N-retinyl-Celite
TL;DR: It is suggested that the retinal matrix shows promise in its application for creating hypoallergenic products and the isolation of high-purity β-lactoglobulin with useful functional properties. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

1994 patent

Method of removing contaminants from a contact lens using electroblotting

Washington, DC: U.S. Patent and Trademark Office.

By: H. Swaisgood & M. Walsh

Source: NC State University Libraries
Added: August 6, 2018

1993 journal article

REVIEW AND UPDATE OF CASEIN CHEMISTRY

JOURNAL OF DAIRY SCIENCE, 76(10), 3054–3061.

By: H. Swaisgood n

author keywords: CASEIN CHEMISTRY; MILK PROTEINS, NOMENCLATURE; SYMPOSIUM
MeSH headings : Amino Acid Sequence; Animals; Calcium / metabolism; Caseins / analysis; Caseins / chemistry; Cattle; Milk Proteins / chemistry; Milk Proteins / genetics; Molecular Structure; Protein Structure, Secondary; Protein Structure, Tertiary; Terminology as Topic
TL;DR: Of all food proteins, bovine milk proteins are probably the most well characterized chemically, physically, and genetically and have significant amounts of secondary and, probably, tertiary structure. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

1992 journal article

Review and update of milk protein chemistry

Journal of Dairy Science, 75, 89.

By: H. Swaisgood

Source: NC State University Libraries
Added: August 6, 2018

1991 journal article

Protein digestibility: In vitro methods of assessment

Advances in Food and Nutrition Research, 35, 185.

By: H. Swaisgood n & G. Catignani n

MeSH headings : Animals; Dietary Proteins / metabolism; Digestion
TL;DR: This chapter describes those changes in protein structure that lead to change in the digestibility of the protein and discusses several methods for assaying protein digestibility. (via Semantic Scholar)
Source: NC State University Libraries
Added: August 6, 2018

1990 journal article

BORDEN SYMPOSIUM ON STABILITY OF MILK-PROTEINS IN DAIRY FOODS

JOURNAL OF DAIRY SCIENCE, 73(12), 3591–3591.

By: H. Swaisgood n & V. Harwalkar*

author keywords: MILK PROTEINS; STABILITY; DAIRY FOODS
TL;DR: Any substantial structural perturbation due to salt, pH, alcohol, or heat treatment leads to irreversible changes in protein structure, even if a simulated physiological environment is restored. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

1989 journal article

Immobilized enzymes as processing aids or analytical tools

ACS Symposium Series, (389), 242.

By: H. Swaisgood n & H. Horton n

UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: NC State University Libraries
Added: August 6, 2018

1989 chapter

Structural changes in milk proteins

In C. A. Barth & E. Schlimme (Eds.), Milk proteins: nutritional, clinical, functional and technological aspects (p. 192).

By: H. Swaisgood n

Ed(s): . C. A. Barth & E. Schlimme

TL;DR: This work has shown that proteins will refold to their native conformation from a random coil state under conditions which mimic the physiological environment and is a consequence of the thermodynamic nature of their structural stabilization. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Source: NC State University Libraries
Added: August 6, 2018

1983 journal article

SPECTROPHOTOMETRIC ASSAY USING ORTHO-PHTHALDIALDEHYDE FOR DETERMINATION OF PROTEOLYSIS IN MILK AND ISOLATED MILK-PROTEINS

JOURNAL OF DAIRY SCIENCE, 66(6), 1219–1227.

By: F. Church  n, H. Swaisgood n, D. Porter n & G. Catignani n

UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

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