Works (47)

Updated: March 10th, 2025 12:14

2007 article

TRYPSIN IMMOBILIZATION ON DERIVATIZED CELLULOSE BEADS BY BIOSPECIFIC AVIDIN-BIOTIN INTERACTION AND CHARACTERIZATION OF THE IMMOBILIZED ACTIVITY1

JANOLINO, V. I. O. L. E. T. A. G., & SWAISGOOD, H. A. R. O. L. D. E. (2007, February 23). Journal of Food Biochemistry.

By: V. Janolino n & H. Swaisgood n

topics (OpenAlex): Biotin and Related Studies; Advanced biosensing and bioanalysis techniques; Electrochemical sensors and biosensors
TL;DR: It is indicated that cellulose beads can be biotinylated for use as bioselective support for trypsin bioreactors and avidin-biotin interaction. (via Semantic Scholar)
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Source: NC State University Libraries
Added: August 6, 2018

2004 article

Cross-Linking and Rheological Changes of Whey Proteins Treated with Microbial Transglutaminase

Truong, V.-D., Clare, D. A., Catignani, G. L., & Swaisgood, H. E. (2004, February 7). Journal of Agricultural and Food Chemistry.

By: V. Truong n, D. Clare n, G. Catignani n & H. Swaisgood n

author keywords: whey protein; immobilized transglutaminase; transglutaminase; protein cross-linking; electrophoresis; Western blots; rheology
MeSH headings : Adsorption; Bacteria / enzymology; Cross-Linking Reagents; Enzymes, Immobilized; Milk Proteins / chemistry; Milk Proteins / metabolism; Rheology; Transglutaminases / metabolism; Whey Proteins
topics (OpenAlex): Proteins in Food Systems; Muscle metabolism and nutrition; biodegradable polymer synthesis and properties
TL;DR: Interestingly, the gelling point of WPI solutions increased from 68 to 94 degrees C after a 4-h reaction, and the gel strength was drastically decreased, suggesting that a process could be developed to produce heat-stable whey proteins for various food applications. (via Semantic Scholar)
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Source: Web Of Science
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2004 article

Nomenclature of the Proteins of Cows’ Milk—Sixth Revision

Farrell, H. M., Jimenez-Flores, R., Bleck, G. T., Brown, E. M., Butler, J. E., Creamer, L. K., … Swaisgood, H. E. (2004, June 1). Journal of Dairy Science.

By: H. Farrell*, R. Jimenez-Flores*, G. Bleck, E. Brown*, J. Butler*, L. Creamer*, C. Hicks*, C. Hollar, K. Ng-Kwai-Hang*, H. Swaisgood n

author keywords: milk protein; structure; nomenclature; review
MeSH headings : Amino Acid Sequence; Animals; Caseins / chemistry; Caseins / classification; Cattle; Immunoglobulins / chemistry; Immunoglobulins / classification; Lactoglobulins; Milk Proteins / chemistry; Milk Proteins / classification; Molecular Sequence Data; Molecular Weight; Sequence Analysis, Protein; Sequence Homology, Amino Acid; Terminology as Topic; Whey Proteins
topics (OpenAlex): Animal Diversity and Health Studies; Milk Quality and Mastitis in Dairy Cows; Glycosylation and Glycoproteins Research
TL;DR: Significant findings related to nomenclature and protein methodology are elucidation of several new genetic variants of the major milk proteins, establishment by sequencing techniques and sequence alignment of the bovine caseins and whey proteins as the reference point for the nomenClature of all homologous milk proteins. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2004 article

Rebuttal on A Semi-Pilot-Scale Procedure for Isolating and Purifying Soybean (Glycine max) Lectin

Fasina, Y. O., Swaisgood, H. E., Garlich, J. D., & Classen, H. L. (2004, April 20). Journal of Agricultural and Food Chemistry.

By: Y. Fasina n, H. Swaisgood n, J. Garlich n & H. Classen n

topics (OpenAlex): Glycosylation and Glycoproteins Research; Carbohydrate Chemistry and Synthesis; Polysaccharides and Plant Cell Walls
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Source: Web Of Science
Added: August 6, 2018

2004 article

The importance of disulfide bridging

Swaisgood, H. E. (2004, December 15). Biotechnology Advances.

By: H. Swaisgood n

MeSH headings : Disulfides / chemistry; Disulfides / metabolism; Food Handling; Lactoglobulins / chemistry; Lactoglobulins / metabolism; Protein Disulfide-Isomerases / chemistry; Protein Disulfide-Isomerases / metabolism; Proteins / chemistry; Proteins / metabolism; Sulfhydryl Compounds / chemistry; Sulfhydryl Compounds / metabolism
topics (OpenAlex): Redox biology and oxidative stress; Insect and Pesticide Research; Genomics, phytochemicals, and oxidative stress
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Source: Web Of Science
Added: August 6, 2018

2003 article

A Semi-Pilot-Scale Procedure for Isolating and Purifying Soybean (Glycine max) Lectin

Fasina, Y. O., Swaisgood, H. E., Garlich, J. D., & Classen, H. L. (2003, July 1). Journal of Agricultural and Food Chemistry.

By: Y. Fasina n, H. Swaisgood n, J. Garlich n & H. Classen n

author keywords: soybean lectin (SBL); Glycine max; lectin purification; scale-up; affinity chromatography; SDS- and native-PAGE
MeSH headings : Chromatography, Affinity; Electrophoresis, Polyacrylamide Gel; Pilot Projects; Plant Lectins / isolation & purification; Soybean Proteins / isolation & purification; Soybeans / chemistry; Ultrafiltration
topics (OpenAlex): Proteins in Food Systems; Phytase and its Applications; Transgenic Plants and Applications
TL;DR: Electrophoretic analysis and hemagglutination assay revealed that the freeze-dried SBL was similar to Sigma-grade SBL in purity and activity (35 and 33 HU/mg protein, respectively). (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2003 article

Biodefense Properties of Milk: The Role of Antimicrobial Proteins and Peptides

Clare, D., Catignani, G., & Swaisgood, H. (2003, June 1). Current Pharmaceutical Design.

By: D. Clare n, G. Catignani* & H. Swaisgood*

author keywords: biodefense proteins and peptides; antimicrobial milk proteins; antimicrobial milk peptides; immunoregulatory milk proteins; immunoregulatory milk peptides; review article
MeSH headings : Adjuvants, Immunologic / chemistry; Adjuvants, Immunologic / pharmacology; Animals; Anti-Bacterial Agents / chemistry; Anti-Bacterial Agents / pharmacology; Colostrum / chemistry; Colostrum / immunology; Humans; Milk / chemistry; Milk / immunology; Milk Proteins / chemistry; Milk Proteins / immunology; Milk Proteins / pharmacology; Milk, Human / chemistry; Milk, Human / immunology; Peptides
topics (OpenAlex): Infant Nutrition and Health; Pediatric health and respiratory diseases; Breastfeeding Practices and Influences
TL;DR: The principal biological properties associated with each of these contributing milk components are reviewed with a special emphasis on the role of biodefensive milk peptides. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2003 article

Investigating the possibility of monitoring lectin levels in commercial soybean meals intended for poultry feeding using steam-heated soybean meal as a model

Fasina, Y. O., Classen, H. L., Garlich, J. D., Swaisgood, H. E., & Clare, D. A. (2003, April 1). Poultry Science.

By: Y. Fasina*, H. Classen*, J. Garlich*, H. Swaisgood* & D. Clare*

author keywords: soybean meal; lectin; urease activity; trypsin inhibitor; protein solubility
MeSH headings : Animal Feed / analysis; Animal Nutritional Physiological Phenomena; Animals; Food Handling / methods; Hydrogen-Ion Concentration; Nutritive Value; Plant Lectins / analysis; Plant Lectins / metabolism; Poultry / metabolism; Soybean Proteins / analysis; Soybean Proteins / metabolism; Temperature; Time Factors; Trypsin Inhibitors / analysis; Urease / metabolism
topics (OpenAlex): Animal Nutrition and Physiology; Proteins in Food Systems; Phytase and its Applications
TL;DR: The denaturation pattern of UA was highly correlated with that of SBL, indicating that UA could be used for monitoring lectin levels in commercial meals, and it was concluded that UA of 0.03 to 0.09 units of pH change are indicative of adequately processed meals that contain negligible Lectin levels. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2003 article

Production and characterization of bio-immobilized keratinase in proteolysis and keratinolysis

Wang, J.-J., Swaisgood, H. E., & Shih, J. C. H. (2003, April 30). Enzyme and Microbial Technology.

By: J. Wang n, H. Swaisgood n & J. Shih n

author keywords: keratinase; bio-immobililzation; streptavidin; fusion protein
topics (OpenAlex): Enzyme Production and Characterization; Animal Genetics and Reproduction; Protein Hydrolysis and Bioactive Peptides
TL;DR: Extracellular production of keratinase–streptavidin fusion protein was accomplished by the cloning of Bacillus subtilis with a transforming plasmid carrying the kerA - stp fusion gene. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2002 article

Bioimmobilization of keratinase using Bacillus subtilis and Escherichia coli systems

Wang, J. J., Swaisgood, H. E., & Shih, J. C. H. (2002, December 17). Biotechnology and Bioengineering.

By: J. Wang n, H. Swaisgood n & J. Shih n

author keywords: keratinase; immobilization; streptavidin; fusion protein
MeSH headings : Bacillus subtilis / classification; Bacillus subtilis / enzymology; Bacillus subtilis / genetics; Cells, Cultured; Enzymes, Immobilized / chemical synthesis; Enzymes, Immobilized / genetics; Enzymes, Immobilized / isolation & purification; Enzymes, Immobilized / metabolism; Escherichia coli / classification; Escherichia coli / enzymology; Gene Expression Regulation, Bacterial; Gene Expression Regulation, Enzymologic; Macromolecular Substances; Peptide Hydrolases / classification; Peptide Hydrolases / genetics; Peptide Hydrolases / isolation & purification; Peptide Hydrolases / metabolism; Protein Binding; Protein Engineering / methods; Recombinant Fusion Proteins / biosynthesis; Recombinant Fusion Proteins / genetics; Species Specificity; Streptavidin / biosynthesis; Streptavidin / classification; Streptavidin / genetics; Streptavidin / isolation & purification
topics (OpenAlex): Enzyme Production and Characterization; Animal Genetics and Reproduction; Bone and Dental Protein Studies
TL;DR: A new genetic construct was developed to produce the keratinase-streptavidin fusion protein, so that the purification and immobilization of the fusion protein onto a biotinylated matrix can be accomplished in a single step. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2002 article

Immobilization and Utilization of the Recombinant Fusion Proteins Trypsin−Streptavidin and Streptavidin−Transglutaminase for Modification of Whey Protein Isolate Functionality

Wilcox, C. P., Clare, D. A., Valentine, V. W., & Swaisgood, H. E. (2002, May 21). Journal of Agricultural and Food Chemistry.

By: C. Wilcox n, D. Clare n, V. Valentine n & H. Swaisgood n

author keywords: immobilization; trypsin; transglutaminase; whey protein; functionality
MeSH headings : Adsorption; Biotinylation; Chemical Phenomena; Chemistry, Physical; Cross-Linking Reagents; DNA, Recombinant; Dithiothreitol / pharmacology; Enzymes, Immobilized; Escherichia coli / genetics; Gels; Glass; Milk Proteins / chemistry; Milk Proteins / metabolism; Recombinant Fusion Proteins; Streptavidin / genetics; Temperature; Transglutaminases / genetics; Transglutaminases / metabolism; Trypsin / genetics; Trypsin / metabolism; Viscosity; Whey Proteins
topics (OpenAlex): Proteins in Food Systems; Blood properties and coagulation; Transgenic Plants and Applications
TL;DR: By combining limited proteolysis with controlled cross-linking, it was possible to create derivatives of whey proteins with enhanced functional properties that allowed for recycling of the enzyme, eliminated the requirement for a downstream inactivation step, and permitted control over the extent of modification. (via Semantic Scholar)
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Source: Web Of Science
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2002 article

Isolation and Partial Characterization of CD36 from Skim Milk

Wilcox, C. P., Janolino, V. G., & Swaisgood, H. E. (2002, August 1). Journal of Dairy Science.

By: C. Wilcox n, V. Janolino n & H. Swaisgood n

author keywords: CD36; milk fat globule membrane; skim milk; sulfhydryl oxidase
MeSH headings : Amino Acid Sequence; Animals; Blotting, Western; CD36 Antigens; Cattle; Centrifugation; Chromatography, Gel; Electrophoresis, Polyacrylamide Gel; Glycolipids / analysis; Glycoproteins / analysis; Glycosylation; Lipid Droplets; Membrane Glycoproteins / chemistry; Membrane Glycoproteins / isolation & purification; Milk / chemistry; Molecular Sequence Data; Molecular Weight; Organic Anion Transporters / chemistry; Organic Anion Transporters / isolation & purification; Ultrafiltration
topics (OpenAlex): Glycosylation and Glycoproteins Research; Polyamine Metabolism and Applications; Protein purification and stability
TL;DR: CD36, a common milk fat globule membrane glycoprotein, was isolated from skim milk by methods similar to those previously utilized for the isolation of sulfhydryl oxidase, giving similar purity as observed by electrophoresis and suggested tissue-specific glycosylation. (via Semantic Scholar)
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Source: Web Of Science
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2002 article

Modification of the Rheological Properties of Whey Protein Isolate through the Use of an Immobilized Microbial Transglutaminase

Wilcox, C. P., & Swaisgood, H. E. (2002, August 30). Journal of Agricultural and Food Chemistry.

By: C. Wilcox n & H. Swaisgood n

author keywords: immobilization; transglutaminase; viscosity; whey protein isolate; gelation; cross linking
MeSH headings : Adsorption; Avidin / chemistry; Bacteria / enzymology; Biotinylation; Cross-Linking Reagents; Enzymes, Immobilized; Glass; Microspheres; Milk Proteins / chemistry; Rheology; Solutions; Transglutaminases / metabolism; Viscosity; Whey Proteins
topics (OpenAlex): Proteins in Food Systems; Polysaccharides Composition and Applications; Microencapsulation and Drying Processes
TL;DR: The functional properties of several batches of WPI were modified using <10 mg of the same enzyme, illustrating the capacity of immobilized enzymes to be used more frequently in applications of this nature. (via Semantic Scholar)
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Source: Web Of Science
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2002 article

Purification and Characterization of the Fusion Protein Trypsin-Streptavidin Expressed in Escherichia coli

Zhao, F., Clare, D. A., Catignani, G. L., & Swaisgood, H. E. (2002, August 1). Journal of Protein Chemistry.

By: F. Zhao n, D. Clare n, G. Catignani n & H. Swaisgood n

author keywords: affinity column; fusion protein; protein expression; protein purification; trypsin; streptavidin
MeSH headings : Blotting, Western; Chromatography, Affinity; Electrophoresis, Polyacrylamide Gel; Escherichia coli / genetics; Recombinant Fusion Proteins / chemistry; Recombinant Fusion Proteins / genetics; Recombinant Fusion Proteins / isolation & purification; Streptavidin / chemistry; Trypsin / chemistry
topics (OpenAlex): Biotin and Related Studies; Click Chemistry and Applications; Monoclonal and Polyclonal Antibodies Research
TL;DR: The molecular size of the soluble purified fusion protein was determined by size-exclusion chromatography using Superose 12 FPLC, indicating that the soluble protein exists as a monomer; thus, the presence of the trypsin domain must prevent the streptavidin domain from tetramer formation. (via Semantic Scholar)
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Source: Web Of Science
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2001 article

Gelation properties of dispersions containing polymerized and native whey protein isolate

Vardhanabhuti, B., Foegeding, E. A., McGuffey, M. K., Daubert, C. R., & Swaisgood, H. E. (2001, January 1). Food Hydrocolloids.

By: B. Vardhanabhuti n, E. Foegeding n, M. McGuffey n, C. Daubert n & H. Swaisgood n

author keywords: whey protein isolate; gelation; rheological properties
topics (OpenAlex): Proteins in Food Systems; Microencapsulation and Drying Processes; biodegradable polymer synthesis and properties
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Source: Web Of Science
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2001 article

IDENTIFICATION OF PEPTIDE LIGANDS GENERATED BY COMBINATORIAL CHEMISTRY THAT BIND α-LACTALBUMIN

Gurgel, P. V., Carbonell, R. G., & Swaisgood, H. E. (2001, August 31). Separation Science and Technology.

By: P. Gurgel n, R. Carbonell n & H. Swaisgood n

topics (OpenAlex): Monoclonal and Polyclonal Antibodies Research; Glycosylation and Glycoproteins Research; Protein purification and stability
TL;DR: The identification of the hexapeptide WHWRKR, obtained from a combinatorial library, that shows affinity for α-lactalbumin is described. (via Semantic Scholar)
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Source: Web Of Science
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2001 article

Molecular design, expression, and affinity immobilization of a trypsin-streptavidin fusion protein☆

Clare, D. A., Valentine, V. W., Catignani, G. L., & Swaisgood, H. E. (2001, April 1). Enzyme and Microbial Technology.

By: D. Clare n, V. Valentine n, G. Catignani n & H. Swaisgood n

author keywords: cloning; protein expression; immobilized enzymes; trypsin
topics (OpenAlex): Biotin and Related Studies; Bacterial Genetics and Biotechnology; Advanced biosensing and bioanalysis techniques
TL;DR: This enzyme bioreactor should serve as an excellent prototype for future studies that will examine the effect of limited proteolysis on functional characteristics of milk proteins, including gelling, emulsifying and foaming properties. (via Semantic Scholar)
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Source: Web Of Science
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2001 patent

Protein ingredient for carrying lipophilic nutrients

Washington, DC: U.S. Patent and Trademark Office.

By: H. Swaisgood, Q. Wang & J. Allen

Source: NC State University Libraries
Added: August 6, 2018

2001 article

Studies of the Binding of α-Lactalbumin to Immobilized Peptide Ligands

Gurgel, P. V., Carbonell, R. G., & Swaisgood, H. E. (2001, November 22). Journal of Agricultural and Food Chemistry.

By: P. Gurgel n, R. Carbonell n & H. Swaisgood n

author keywords: alpha-lactalbumin; peptide ligands; bioselective adsorption; binding mechanism
MeSH headings : Adsorption; Amino Acid Sequence; Lactalbumin / metabolism; Ligands; Mutagenesis, Site-Directed; Oligopeptides / metabolism; Protein Binding; Protein Precursors / metabolism; Recombinant Proteins / metabolism; Resins, Plant; Thermodynamics
topics (OpenAlex): Chemical Synthesis and Analysis; Protein purification and stability; Infant Nutrition and Health
TL;DR: The present work investigates the mechanism of binding of α-lactalbumin to the peptide ligand WH WRKR and its variants HWRKR and acetylated WHWRKR immobilized on a polymethacrylate chromatographic resin. (via Semantic Scholar)
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2000 article

Bioactive Milk Peptides: A Prospectus

Clare, D. A., & Swaisgood, H. E. (2000, June 1). Journal of Dairy Science.

By: D. Clare n & H. Swaisgood n

author keywords: bioactive peptide; milk proteins; functional foods
MeSH headings : Angiotensin-Converting Enzyme Inhibitors / chemistry; Angiotensin-Converting Enzyme Inhibitors / metabolism; Animals; Cattle; Fibrinolytic Agents / chemistry; Fibrinolytic Agents / metabolism; Milk / microbiology; Milk / physiology; Milk Proteins / chemistry; Milk Proteins / pharmacology; Milk Proteins / therapeutic use; Opioid Peptides / chemistry; Opioid Peptides / physiology; Peptides / analysis; Peptides / physiology
topics (OpenAlex): Protein Hydrolysis and Bioactive Peptides; Insect Utilization and Effects; Meat and Animal Product Quality
TL;DR: This work reviews the scientific literature and attempts to stimulate consideration of the continued use of bioactive peptides and their expanded development as a commercial product. (via Semantic Scholar)
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Source: Web Of Science
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2000 article

Fractionation of whey proteins with a hexapeptide ligand affinity resin

Gurgel, P. V., Carbonell, R. G., & Swaisgood, H. E. (2000, November 1). Bioseparation.

By: P. Gurgel n, R. Carbonell n & H. Swaisgood n

author keywords: alpha-lactalbumin; bioselective adsorption; peptide ligands; whey protein isolate
MeSH headings : Animals; Cattle; Chromatography, Affinity / methods; Lactalbumin / isolation & purification; Ligands; Milk Proteins / isolation & purification; Oligopeptides; Resins, Synthetic; Whey Proteins
topics (OpenAlex): Protein Hydrolysis and Bioactive Peptides; Infant Nutrition and Health; Biopolymer Synthesis and Applications
TL;DR: This study describes two methods for production of α-lactalbumin from whey protein isolate using bioselective adsorption and the combined use of an amino resin followed by the WHWRKR resin produce a highly purified α- lact Albumin (100%). (via Semantic Scholar)
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2000 article

Thermodynamics of Unfolding of β-Trypsin at pH 2.8

Brumano, M. H. N., Rogana, E., & Swaisgood, H. E. (2000, October 1). Archives of Biochemistry and Biophysics.

By: M. Brumano*, E. Rogana* & H. Swaisgood n

MeSH headings : Animals; Calorimetry; Cattle; Circular Dichroism; Hydrogen-Ion Concentration; Protein Folding; Spectrometry, Fluorescence; Spectrophotometry; Temperature; Thermodynamics; Trypsin / chemistry; Trypsin / metabolism; Ultraviolet Rays; Urea / pharmacology
topics (OpenAlex): Spectroscopy and Quantum Chemical Studies; Protein Structure and Dynamics; Protein Interaction Studies and Fluorescence Analysis
TL;DR: The value for the stability of β-trypsin estimated from spectroscopic data is in reasonable agreement with the value calculated from urea titration curves, which supports the two-state model for the unfolding of this protein. (via Semantic Scholar)
Source: NC State University Libraries
Added: August 6, 2018

1999 article

Binding of Lipophilic Nutrients to β-Lactoglobulin Prepared by Bioselective Adsorption

Wang, Q., Allen, J. C., & Swaisgood, H. E. (1999, February 1). Journal of Dairy Science.

By: Q. Wang n, J. Allen n & H. Swaisgood n

author keywords: binding lipophilic nutrients; beta-lactoglobulin; bioselective adsorption
MeSH headings : Adsorption; Binding, Competitive; Diterpenes; Ergocalciferols / metabolism; Hydrogen-Ion Concentration; Lactoglobulins / metabolism; Retinaldehyde / metabolism; Retinyl Esters; Spectrometry, Fluorescence; Tryptophan / chemistry; Vitamin A / analogs & derivatives; Vitamin A / metabolism
topics (OpenAlex): Biochemical Analysis and Sensing Techniques; Vitamin D Research Studies; Regulation of Appetite and Obesity
TL;DR: The results are consistent with the model in which the retinoids and vitamin D2 bind in the calyx formed by the beta-barrel; palmitate and a second molecule of vitamin D 2 bind in a surface pocket near the dimer contact region. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

1999 article

Modification of rheological properties of whey protein isolates by limited proteolysis

Huang, X. L., Catignani, G. L., & Swaisgood, H. E. (1999, March 1). Food / Nahrung.

By: X. Huang n, G. Catignani n & H. Swaisgood*

topics (OpenAlex): Proteins in Food Systems; Microencapsulation and Drying Processes; Muscle metabolism and nutrition
Source: Web Of Science
Added: August 6, 2018

1998 article

Calcium Binding of Phosphopeptides Derived from Hydrolysis of a αs-Casein or β-Casein Using Immobilized Trypsin

Park, O., Swaisgood, H. E., & Allen, J. C. (1998, November 1). Journal of Dairy Science.

By: O. Park n, H. Swaisgood n & J. Allen n

author keywords: casein; immobilized trypsin; casein phosphopeptide; calcium binding
MeSH headings : Amino Acid Sequence; Animals; Binding Sites; Calcium / metabolism; Caseins / chemistry; Caseins / metabolism; Enzymes, Immobilized; Hydrolysis; Milk / chemistry; Molecular Sequence Data; Peptide Fragments / metabolism; Phosphopeptides / metabolism; Trypsin / metabolism
topics (OpenAlex): Protein Hydrolysis and Bioactive Peptides; Meat and Animal Product Quality; Proteins in Food Systems
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1998 article

Cloning and expression of a streptavidin-lipase fusion gene in Escherichia coli and characterization of the immobilized fusion protein

Lee, P., & Swaisgood, H. E. (1998, March 1). Enzyme and Microbial Technology.

By: P. Lee n & H. Swaisgood n

author keywords: streptavidin-lipase fusion gene; fusion protein; Escherichia coli; cloning; transesterification
topics (OpenAlex): Biotin and Related Studies; Enzyme Catalysis and Immobilization; Bacterial Genetics and Biotechnology
TL;DR: Streptavidin-lipase fusion protein was purified and immobilized in a single step from recombinant cell lysates by bioselective adsorption on 6-(biotinamido)hexanamidopropyl-controlled pore glass and exhibited a pH optimum between 7 and 8 and a preference for short-chain fatty acids. (via Semantic Scholar)
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1998 journal article

Construction and expression of a bifunctional single-chain antibody against Bacillus cereus spores

Applied and Environmental Microbiology, 64(7), 2490–2496.

By: K. Koo, P. Foegeding & H. Swaisgood

Source: NC State University Libraries
Added: August 6, 2018

1998 journal article

Development of a streptavidin-conjugated single-chain antibody that binds Bacillus cereus spores

Applied and Environmental Microbiology, 64(7), 2497–2502.

By: K. Koo, P. Foegeding & H. Swaisgood

Source: NC State University Libraries
Added: August 6, 2018

1998 article

Isolation of RNA and DNA fragments using diatomaceous earth

Koo, K., Foegeding, P. M., & Swaisgood, H. E. (1998, July 1). Biotechnology Techniques.

By: K. Koo, P. Foegeding* & H. Swaisgood*

topics (OpenAlex): Plant Disease Resistance and Genetics; Molecular Biology Techniques and Applications; Bacteriophages and microbial interactions
TL;DR: A series of simple and phenol-free silica-based protocols for isolating and cleaning RNA or DNA fragments from different sources were developed and Cytoplasmic RNA isolated from hybridoma cells by this method was used in RT-PCR. (via Semantic Scholar)
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1998 article

Protein Concentration Dependence of Palmitate Binding to β-Lactoglobulin

Wang, Q., Allen, J. C., & Swaisgood, H. E. (1998, January 1). Journal of Dairy Science.

By: Q. Wang n, J. Allen n & H. Swaisgood n

author keywords: beta-lactoglobulin; palmitate; palmitate binding
topics (OpenAlex): Proteins in Food Systems; Muscle metabolism and nutrition; Phytase and its Applications
TL;DR: Formation of the dimer, by hydrophobic interactions at the monomers contact site, eliminated palmitate binding sites on the monomer but formed a higher affinity pocket for binding to theDimer. (via Semantic Scholar)
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1997 article

Binding of Retinoids to β-Lactoglobulin Isolated by Bioselective Adsorption

Wang, Q., Allen, J. C., & Swaisgood, H. E. (1997, June 1). Journal of Dairy Science.

By: Q. Wang n, J. Allen n & H. Swaisgood n

author keywords: beta-lactoglobulin; retinoid binding; bioselective adsorption; whey protein isolate
MeSH headings : Adsorption; Animals; Cattle; Chromatography, Gel / methods; Chromatography, Gel / veterinary; Female; Hydrogen-Ion Concentration; Lactoglobulins / analysis; Lactoglobulins / chemistry; Lactoglobulins / metabolism; Milk / chemistry; Milk Proteins / analysis; Protein Binding; Retinoids / chemistry; Retinoids / metabolism; Spectrometry, Fluorescence / methods; Spectrometry, Fluorescence / veterinary; Whey Proteins
topics (OpenAlex): Proteins in Food Systems; Biochemical Analysis and Sensing Techniques; Antioxidant Activity and Oxidative Stress
TL;DR: Binding of β-lactoglobulin (LG) that had been prepared by bioselective adsorption on N-retinyl-Celite TM was determined from changes in the fluorescence quenching of the protein tryptophanyl residues, suggesting that a tryptophile is in the binding site. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

1997 article

Binding of Vitamin D and Cholesterol to β-Lactoglobulin

Wang, Q., Allen, J. C., & Swaisgood, H. E. (1997, June 1). Journal of Dairy Science.

By: Q. Wang n, J. Allen n & H. Swaisgood n

author keywords: beta-lactoglobulin; vitamin D binding; cholesterol binding; bioselective adsorption
MeSH headings : Adsorption; Animals; Cattle; Cholecalciferol / chemistry; Cholecalciferol / metabolism; Cholesterol / chemistry; Cholesterol / metabolism; Chromatography, Gel / methods; Chromatography, Gel / veterinary; Dehydrocholesterols / metabolism; Ergocalciferols / chemistry; Ergocalciferols / metabolism; Ergosterol / chemistry; Ergosterol / metabolism; Female; Lactoglobulins / chemistry; Lactoglobulins / metabolism; Protein Binding; Retinoids / chemistry; Retinoids / metabolism; Spectrometry, Fluorescence / methods; Spectrometry, Fluorescence / veterinary; Vitamin D / chemistry; Vitamin D / metabolism
topics (OpenAlex): Proteins in Food Systems; Muscle metabolism and nutrition; Vitamin D Research Studies
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UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

1997 article

Characterization of a Chemically Conjugated Lipase Bioreactor

Lee, P., & Swaisgood, H. E. (1997, August 1). Journal of Agricultural and Food Chemistry.

By: P. Lee n & H. Swaisgood n

author keywords: biotinylated lipase; immobilized lipase; immobilized avidin; interesterification
topics (OpenAlex): Enzyme Catalysis and Immobilization; Analytical Chemistry and Chromatography; Electrochemical sensors and biosensors
TL;DR: The hydrolytic specific activity of lipase immobilization on CPG-3000 and on nonporous beads was essentially the same as that for the biotinylated free enzyme, whereas that immobilized on C PG-2000 was about 50% less. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

1997 article

Comparison of the properties of trypsin immobilized on 2 Celite™ derivatives1Paper No. FSR 95-4 of the Journal Series of the Department of Food Science, North Carolina State University, Raleigh, NC 27695-7624. The use of trade names in this publication does not imply endorsement by the North Carolina Agricultural Research Service of the products named, nor criticism of similar ones not mentioned.1

Huang, X. L., Catignani, G. L., & Swaisgood, H. E. (1997, February 1). Journal of Biotechnology.

By: X. Huang n, G. Catignani n & H. Swaisgood n

author keywords: immobilized trypsin; succinamidopropyl-Celite; aminopropyl-Celite; beta-lactoglobulin
MeSH headings : Diatomaceous Earth / chemistry; Enzymes, Immobilized / chemistry; Enzymes, Immobilized / metabolism; Hydrogen-Ion Concentration; Kinetics; Trypsin / chemistry; Trypsin / metabolism
topics (OpenAlex): Phytase and its Applications; Enzyme Production and Characterization; Protein Hydrolysis and Bioactive Peptides
TL;DR: The two methods of immobilization on Celite gave biocataysts with similar kinetic properties but immobilized on SAPC yielded slightly higher loadings and higher specific activities. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

1997 article

Development and Characterization of a Bioselective Adsorption Matrix for Removal of Bacillus cereus Spores from Buffer and Milk

Darquea, D., Swaisgood, H. E., & Foegeding, P. M. (1997, December 1). LWT.

By: D. Darquea n, H. Swaisgood n & P. Foegeding n

topics (OpenAlex): Protein purification and stability; Monoclonal and Polyclonal Antibodies Research; Enzyme Production and Characterization
Source: NC State University Libraries
Added: August 6, 2018

1997 article

Micro‐scale Method for Determining Foaming Properties of Protein

HUANG, X. I. A. O. L. I. N. L., CATIGNANI, G. E. O. R. G. E. L., & SWAISGOOD, H. A. R. O. L. D. E. (1997, September 1). Journal of Food Science.

By: X. Huang n, G. Catignani n & H. Swaisgood n

author keywords: microscale whipping method; foam properties; protein
topics (OpenAlex): Proteins in Food Systems; Pickering emulsions and particle stabilization; Surfactants and Colloidal Systems
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Source: Web Of Science
Added: August 6, 2018

1997 article

Modification of Milkfat Physical Properties by ImmobilizedPseudomonas fluorescensLipase

Lee, P., & Swaisgood, H. E. (1997, August 1). Journal of Agricultural and Food Chemistry.

By: P. Lee n & H. Swaisgood n

author keywords: milkfat; immobilized lipase; streptavidin-lipase; fusion protein; interesterification
topics (OpenAlex): Enzyme Catalysis and Immobilization; Food Chemistry and Fat Analysis; Microbial Metabolic Engineering and Bioproduction
TL;DR: A bifunctional fusion protein was constructed by fusion of the streptavidin gene from Streptomyces avidinii to the lipase gene from Pseudomonas fluorescens, and the resulting strePTavidin−lipase was expressed in Escherichia coli. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

1997 article

Production and Purification of an Active Bovine Lysozyme in Tobacco (Nicotiana tabacum):  Utilization of Value-Added Crop Plants Traditionally Grown under Intensive Agriculture

Wilcox, C. P., Weissinger, A. K., Long, R. C., Fitzmaurice, L. C., Mirkov, T. E., & Swaisgood, H. E. (1997, July 1). Journal of Agricultural and Food Chemistry.

By: C. Wilcox n, A. Weissinger n, R. Long n, L. Fitzmaurice n, T. Mirkov n & H. Swaisgood n

author keywords: transgenic; lysozyme; recombinant protein; antimicrobial; Nicotiana tabacum
topics (OpenAlex): Transgenic Plants and Applications; Enzyme Production and Characterization; Animal Genetics and Reproduction
TL;DR: It was concluded that tobacco was an excellent choice for expression of the recombinant protein and that the purification process developed in this study demonstrates methodology for isolation of high-value enzymes from tobacco and other crop plants. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

1997 article

Purity and Yield of β-Lactoglobulin Isolated by an N-Retinyl-Celite Bioaffinity Column

Heddleson, R. A., Allen, J. C., Wang, Q., & Swaisgood, H. E. (1997, July 1). Journal of Agricultural and Food Chemistry.

By: R. Heddleson n, J. Allen n, Q. Wang n & H. Swaisgood n

author keywords: beta-lactoglobulin; alpha-lactalbumin; bioselective adsorption; N-retinyl-Celite
topics (OpenAlex): Infant Nutrition and Health; Enzyme Production and Characterization; Proteins in Food Systems
TL;DR: It is suggested that the retinal matrix shows promise in its application for creating hypoallergenic products and the isolation of high-purity β-lactoglobulin with useful functional properties. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

1994 patent

Method of removing contaminants from a contact lens using electroblotting

Washington, DC: U.S. Patent and Trademark Office.

By: H. Swaisgood & M. Walsh

Source: NC State University Libraries
Added: August 6, 2018

1993 article

Review and Update of Casein Chemistry

Swaisgood, H. E. (1993, October 1). Journal of Dairy Science.

By: H. Swaisgood n

author keywords: CASEIN CHEMISTRY; MILK PROTEINS, NOMENCLATURE; SYMPOSIUM
MeSH headings : Amino Acid Sequence; Animals; Calcium / metabolism; Caseins / analysis; Caseins / chemistry; Cattle; Milk Proteins / chemistry; Milk Proteins / genetics; Molecular Structure; Protein Structure, Secondary; Protein Structure, Tertiary; Terminology as Topic
topics (OpenAlex): Proteins in Food Systems; Protein Hydrolysis and Bioactive Peptides; Probiotics and Fermented Foods
TL;DR: Of all food proteins, bovine milk proteins are probably the most well characterized chemically, physically, and genetically and have significant amounts of secondary and, probably, tertiary structure. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

1992 journal article

Review and update of milk protein chemistry

Journal of Dairy Science, 75, 89.

By: H. Swaisgood

Source: NC State University Libraries
Added: August 6, 2018

1991 article

Protein Digestibility: In Vitro Methods of Assessment

Swaisgood, H. E., & Catignani, G. L. (1991, January 1). Advances in Food and Nutrition Research.

By: H. Swaisgood n & G. Catignani n

MeSH headings : Animals; Dietary Proteins / metabolism; Digestion
topics (OpenAlex): Muscle metabolism and nutrition; Proteins in Food Systems; Protein Hydrolysis and Bioactive Peptides
TL;DR: This chapter describes those changes in protein structure that lead to change in the digestibility of the protein and discusses several methods for assaying protein digestibility. (via Semantic Scholar)
Source: NC State University Libraries
Added: August 6, 2018

1990 article

Borden Symposium on Stability of Milk Proteins in Dairy Foods

Swaisgood, H. E., & Harwalkar, V. R. (1990, December 1). Journal of Dairy Science.

By: H. Swaisgood n & V. Harwalkar*

author keywords: MILK PROTEINS; STABILITY; DAIRY FOODS
topics (OpenAlex): Proteins in Food Systems; Enzyme Structure and Function; Probiotics and Fermented Foods
TL;DR: Any substantial structural perturbation due to salt, pH, alcohol, or heat treatment leads to irreversible changes in protein structure, even if a simulated physiological environment is restored. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

1989 article

Immobilized Enzymes as Processing Aids or Analytical Tools

Swaisgood, H. E., & Horton, H. R. (1989, January 1). ACS Symposium Series.

By: H. Swaisgood n & H. Horton n

topics (OpenAlex): Enzyme Catalysis and Immobilization; Enzyme Production and Characterization; Biofuel production and bioconversion
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UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: NC State University Libraries
Added: August 6, 2018

1989 article

Structural Changes in Milk Proteins

Swaisgood, H. E. (1989, January 1). Steinkopff EBooks.

By: H. Swaisgood n

Ed(s):

topics (OpenAlex): Protein Structure and Dynamics; Enzyme Structure and Function; RNA and protein synthesis mechanisms
TL;DR: This work has shown that proteins will refold to their native conformation from a random coil state under conditions which mimic the physiological environment and is a consequence of the thermodynamic nature of their structural stabilization. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Source: NC State University Libraries
Added: August 6, 2018

1983 article

Spectrophotometric Assay Using o-Phthaldialdehyde for Determination of Proteolysis in Milk and Isolated Milk Proteins

Church, F. C., Swaisgood, H. E., Porter, D. H., & Catignani, G. L. (1983, June 1). Journal of Dairy Science.

By: F. Church n, H. Swaisgood n, D. Porter n & G. Catignani n

topics (OpenAlex): Protein Hydrolysis and Bioactive Peptides; Meat and Animal Product Quality; Proteins in Food Systems
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

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