Works (1)
Updated: July 5th, 2023 15:55
2006 journal article
A counterintuitive Mg2+-dependent and modification-assisted functional folding of mitochondrial tRNAs
JOURNAL OF MOLECULAR BIOLOGY, 362(4), 771–786.
author keywords: mitochondrial RNA folding; aminoacylation; tRNA(Met); tRNA(Leu)
MeSH headings : Aminoacylation; Animals; Base Sequence; Cattle; Circular Dichroism; Electrophoretic Mobility Shift Assay; Escherichia coli / chemistry; Magnesium / metabolism; Magnesium / pharmacology; Molecular Sequence Data; Nucleic Acid Conformation / drug effects; Nucleic Acid Denaturation; RNA / chemistry; RNA / genetics; RNA Stability / drug effects; RNA, Mitochondrial; RNA, Transfer / chemistry; RNA, Transfer / genetics; Temperature; Yeasts / chemistry
TL;DR:
Investigation of functional folding interactions of chemically synthesized and site-specifically modified mitochondrial and cytoplasmic tRNAs finds that Mg2+ is critical for folding of the truncated D-domain of bovine mtRNAMet with the tRNA's T-domain and indicates that conserved modifications assist and stabilize the formation of the functional mtRNA tertiary structure.
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