Janet L Shurtleff

author keywords: Artemisinin; Artemisia annua; HPLC-MS; Metabolic profiling; Self-pollination; Genetic engineering

MeSH headings : Alkyl and Aryl Transferases / genetics; Antimalarials / isolation & purification; Antimalarials / metabolism; Artemisia annua / anatomy & histology; Artemisia annua / chemistry; Artemisia annua / enzymology; Artemisia annua / physiology; Artemisinins / isolation & purification; Artemisinins / metabolism; Chromatography, High Pressure Liquid; Cytochrome P-450 Enzyme System / genetics; Flowers / anatomy & histology; Flowers / chemistry; Flowers / enzymology; Flowers / physiology; Lactones / isolation & purification; Lactones / metabolism; Plant Leaves / anatomy & histology; Plant Leaves / chemistry; Plant Leaves / enzymology; Plant Leaves / physiology; Plant Proteins / genetics; Plants, Medicinal; Pollination / physiology; Seedlings / anatomy & histology; Seedlings / chemistry; Seedlings / enzymology; Seedlings / physiology; Self-Fertilization / physiology; Spectrometry, Mass, Electrospray Ionization; Time Factors

TL;DR: High performance liquid chromatography–mass spectrometry-based metabolic profiling analyses showed that leaves, flowers, and young seedlings of F2 plants produced artemisinin, and RT-PCR analysis showed that progeny of self-pollinated plants expressed the amorpha-4, 11-diene synthase (ADS) and cytochrome P450 monooxygenase 71 AV1 (CYP71AV1) genes, which are involved in art Artemisia annua (via Semantic Scholar)