Jessica Torres-Kolbus

Works (6)

Updated: July 5th, 2023 15:46

2014 journal article

Genetically encoded unstrained olefins for live cell labeling with tetrazine dyes

CHEMICAL COMMUNICATIONS, 50(86), 13085–13088.

By: Y. Lee *, Y. Kurra*, Y. Yang *, J. Torres-Kolbus n, A. Deiters n & W. Liu *

MeSH headings : Alkenes / chemistry; Amino Acids / metabolism; Amino Acyl-tRNA Synthetases / genetics; Amino Acyl-tRNA Synthetases / metabolism; Bacterial Outer Membrane Proteins / chemistry; Bacterial Outer Membrane Proteins / metabolism; Cycloaddition Reaction; Escherichia coli / metabolism; Escherichia coli Proteins / chemistry; Escherichia coli Proteins / metabolism; Fluorescein / chemistry; Fluorescent Dyes / chemistry; Green Fluorescent Proteins / chemistry; Heterocyclic Compounds, 1-Ring / chemistry; Hydrolases / chemistry; Hydrolases / metabolism; Mutation; RNA, Transfer / metabolism
TL;DR: A number of non-canonical amino acids (NCAAs) with unstrained olefins are genetically encoded using mutant pyrrolysyl-t RNA synthetase-tRNA(Pyl)(CUA) pairs, leading to selective labeling of proteins bearing these NCAAs in live cells. (via Semantic Scholar)
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Added: August 6, 2018

2014 article

Genetically encoded unstrained olefins for live cell labeling with tetrazine dyes (vol 50, pg 13085, 2014)

Lee, Y.-J., Kurra, Y., Yang, Y., Torres-Kolbus, J., Deiters, A., & Liu, W. R. (2014, November 11). CHEMICAL COMMUNICATIONS, Vol. 50, pp. 13396–13396.

By: Y. Lee *, Y. Kurra*, Y. Yang *, J. Torres-Kolbus n, A. Deiters n & W. Liu *

TL;DR: Genetically encoded unstrained olefins for live cell labeling with tetrazine dyes is shown to be a viable alternative for high-performance liquid chromatography of Na6(CO3)(SO4)2, Na2SO4, and Na2CO3 for cell labeling. (via Semantic Scholar)
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Added: August 6, 2018

2014 journal article

Synthesis of Non-linear Protein Dimers through a Genetically Encoded Thiol-ene Reaction

PLOS ONE, 9(9).

By: J. Torres-Kolbus n, C. Chou n, J. Liu * & A. Deiters n

MeSH headings : Alkenes / metabolism; Animals; Fluorescent Dyes / metabolism; Green Fluorescent Proteins / metabolism; Lysine / metabolism; Methanosarcina barkeri / metabolism; Muramidase / metabolism; Myoglobin / metabolism; Protein Multimerization; Sulfhydryl Compounds / metabolism
TL;DR: The genetic encoding of a set of alkene lysines is demonstrated using the orthogonal PylRS/PylTCUA pair in Escherichia coli, showing the applicability of this approach in the formation of heterogeneous non-linear fused proteins. (via Semantic Scholar)
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Added: August 6, 2018

2014 journal article

Two Rapid Catalyst-Free Click Reactions for In Vivo Protein Labeling of Genetically Encoded Strained Alkene/Alkyne Functionalities

BIOCONJUGATE CHEMISTRY, 25(9), 1730–1738.

By: Y. Kurra*, K. Odoi*, Y. Lee *, Y. Yang *, T. Lu*, S. Wheeler *, J. Torres-Kolbus n, A. Deiters n, W. Liu *

MeSH headings : Alkenes / chemistry; Alkynes / chemistry; Catalysis; Click Chemistry; Cyclooctanes / chemistry; Escherichia coli Proteins / chemistry; Kinetics; Models, Molecular; Protein Conformation; Proteins / chemistry; Staining and Labeling / methods; Tyrosine / chemistry
TL;DR: To apply these “click” reactions for site-specific protein labeling, five tyrosine derivatives that contain a norbornene, trans-cyclooctene, or cyclooctyne entity were genetically encoded into proteins in Escherichia coli using an engineered pyrrolysyl-tRNA synthetase-tRNACUAPyl pair. (via Semantic Scholar)
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2012 journal article

Genetically encoded norbornene directs site-specific cellular protein labelling via a rapid bioorthogonal reaction

NATURE CHEMISTRY, 4(4), 298–304.

By: K. Lang *, L. Davis*, J. Torres-Kolbus n, C. Chou n, A. Deiters n & J. Chin *

MeSH headings : Amino Acids / metabolism; Amino Acyl-tRNA Synthetases / metabolism; Cell Line; Fluorescent Dyes / chemistry; Green Fluorescent Proteins / genetics; Green Fluorescent Proteins / metabolism; Humans; Lysine / analogs & derivatives; Lysine / metabolism; Norbornanes / chemistry; Proteins / genetics; Proteins / metabolism; Rhodamines / chemistry
TL;DR: The genetic encoding of a norbornene amino acid using the pyrrolysyl tRNA synthetase/tRNA(CUA) pair in Escherichia coli and mammalian cells and the rapid bioorthogonal site-specific labelling of a protein on the mammalian cell surface is demonstrated. (via Semantic Scholar)
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Added: August 6, 2018

2012 journal article

Photocontrol of Tyrosine Phosphorylation in Mammalian Cells via Genetic Encoding of Photocaged Tyrosine

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 134(29), 11912–11915.

By: E. Arbely *, J. Torres-Kolbus n, A. Deiters n & J. Chin *

MeSH headings : Amino Acyl-tRNA Synthetases / genetics; Escherichia coli / genetics; Escherichia coli / metabolism; HEK293 Cells; Humans; Light; Methanosarcina / enzymology; Methanosarcina / genetics; Mutation; Phosphorylation; Protein Engineering / methods; STAT1 Transcription Factor / genetics; STAT1 Transcription Factor / metabolism; Signal Transduction; Tyrosine / genetics; Tyrosine / metabolism
TL;DR: By photocaging Tyr701 of STAT1, it is demonstrated that it is possible to photocontrol tyrosine phosphorylation and signal transduction in mammalian cells. (via Semantic Scholar)
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