Works (6)

Updated: July 5th, 2023 15:46

2014 article

Correction: Genetically encoded unstrained olefins for live cell labeling with tetrazine dyes

Lee, Y.-J., Kurra, Y., Yang, Y., Torres-Kolbus, J., Deiters, A., & Liu, W. R. (2014, January 1). Chemical Communications.

By: Y. Lee*, Y. Kurra*, Y. Yang*, J. Torres-Kolbus n, A. Deiters n & W. Liu*

topics (OpenAlex): bioluminescence and chemiluminescence research; Click Chemistry and Applications
TL;DR: Genetically encoded unstrained olefins for live cell labeling with tetrazine dyes is shown to be a viable alternative for high-performance liquid chromatography of Na6(CO3)(SO4)2, Na2SO4, and Na2CO3 for cell labeling. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2014 article

Genetically encoded unstrained olefins for live cell labeling with tetrazine dyes

Lee, Y.-J., Kurra, Y., Yang, Y., Torres-Kolbus, J., Deiters, A., & Liu, W. R. (2014, January 1). Chemical Communications.

By: Y. Lee*, Y. Kurra*, Y. Yang*, J. Torres-Kolbus n, A. Deiters n & W. Liu*

MeSH headings : Alkenes / chemistry; Amino Acids / metabolism; Amino Acyl-tRNA Synthetases / genetics; Amino Acyl-tRNA Synthetases / metabolism; Bacterial Outer Membrane Proteins / chemistry; Bacterial Outer Membrane Proteins / metabolism; Cycloaddition Reaction; Escherichia coli / metabolism; Escherichia coli Proteins / chemistry; Escherichia coli Proteins / metabolism; Fluorescein / chemistry; Fluorescent Dyes / chemistry; Green Fluorescent Proteins / chemistry; Heterocyclic Compounds, 1-Ring / chemistry; Hydrolases / chemistry; Hydrolases / metabolism; Mutation; RNA, Transfer / metabolism
topics (OpenAlex): Click Chemistry and Applications; Chemical Synthesis and Analysis; Advanced biosensing and bioanalysis techniques
TL;DR: A number of non-canonical amino acids (NCAAs) with unstrained olefins are genetically encoded using mutant pyrrolysyl-t RNA synthetase-tRNA(Pyl)(CUA) pairs, leading to selective labeling of proteins bearing these NCAAs in live cells. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2014 article

Synthesis of Non-linear Protein Dimers through a Genetically Encoded Thiol-ene Reaction

Torres-Kolbus, J., Chou, C., Liu, J., & Deiters, A. (2014, September 2). PLoS ONE.

By: J. Torres-Kolbus n, C. Chou n, J. Liu* & A. Deiters n

MeSH headings : Alkenes / metabolism; Animals; Fluorescent Dyes / metabolism; Green Fluorescent Proteins / metabolism; Lysine / metabolism; Methanosarcina barkeri / metabolism; Muramidase / metabolism; Myoglobin / metabolism; Protein Multimerization; Sulfhydryl Compounds / metabolism
topics (OpenAlex): Click Chemistry and Applications; Chemical Synthesis and Analysis; Fluorine in Organic Chemistry
TL;DR: The genetic encoding of a set of alkene lysines is demonstrated using the orthogonal PylRS/PylTCUA pair in Escherichia coli, showing the applicability of this approach in the formation of heterogeneous non-linear fused proteins. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2014 article

Two Rapid Catalyst-Free Click Reactions for In Vivo Protein Labeling of Genetically Encoded Strained Alkene/Alkyne Functionalities

Kurra, Y., Odoi, K. A., Lee, Y.-J., Yang, Y., Lu, T., Wheeler, S. E., … Liu, W. R. (2014, August 26). Bioconjugate Chemistry.

By: Y. Kurra*, K. Odoi*, Y. Lee*, Y. Yang*, T. Lu*, S. Wheeler*, J. Torres-Kolbus n, A. Deiters n, W. Liu*

MeSH headings : Alkenes / chemistry; Alkynes / chemistry; Catalysis; Click Chemistry; Cyclooctanes / chemistry; Escherichia coli Proteins / chemistry; Kinetics; Models, Molecular; Protein Conformation; Proteins / chemistry; Staining and Labeling / methods; Tyrosine / chemistry
topics (OpenAlex): Click Chemistry and Applications; Monoclonal and Polyclonal Antibodies Research; Chemical Synthesis and Analysis
TL;DR: To apply these “click” reactions for site-specific protein labeling, five tyrosine derivatives that contain a norbornene, trans-cyclooctene, or cyclooctyne entity were genetically encoded into proteins in Escherichia coli using an engineered pyrrolysyl-tRNA synthetase-tRNACUAPyl pair. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2012 article

Genetically encoded norbornene directs site-specific cellular protein labelling via a rapid bioorthogonal reaction

Lang, K., Davis, L., Torres-Kolbus, J., Chou, C., Deiters, A., & Chin, J. W. (2012, February 3). Nature Chemistry.

By: K. Lang*, L. Davis*, J. Torres-Kolbus n, C. Chou n, A. Deiters n & J. Chin*

MeSH headings : Amino Acids / metabolism; Amino Acyl-tRNA Synthetases / metabolism; Cell Line; Fluorescent Dyes / chemistry; Green Fluorescent Proteins / genetics; Green Fluorescent Proteins / metabolism; Humans; Lysine / analogs & derivatives; Lysine / metabolism; Norbornanes / chemistry; Proteins / genetics; Proteins / metabolism; Rhodamines / chemistry
topics (OpenAlex): Click Chemistry and Applications; Monoclonal and Polyclonal Antibodies Research; Chemical Synthesis and Analysis
TL;DR: The genetic encoding of a norbornene amino acid using the pyrrolysyl tRNA synthetase/tRNA(CUA) pair in Escherichia coli and mammalian cells and the rapid bioorthogonal site-specific labelling of a protein on the mammalian cell surface is demonstrated. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2012 article

Photocontrol of Tyrosine Phosphorylation in Mammalian Cells via Genetic Encoding of Photocaged Tyrosine

Arbely, E., Torres-Kolbus, J., Deiters, A., & Chin, J. W. (2012, July 3). Journal of the American Chemical Society.

By: E. Arbely*, J. Torres-Kolbus n, A. Deiters n & J. Chin*

MeSH headings : Amino Acyl-tRNA Synthetases / genetics; Escherichia coli / genetics; Escherichia coli / metabolism; HEK293 Cells; Humans; Light; Methanosarcina / enzymology; Methanosarcina / genetics; Mutation; Phosphorylation; Protein Engineering / methods; STAT1 Transcription Factor / genetics; STAT1 Transcription Factor / metabolism; Signal Transduction; Tyrosine / genetics; Tyrosine / metabolism
topics (OpenAlex): RNA and protein synthesis mechanisms; interferon and immune responses; Cytokine Signaling Pathways and Interactions
TL;DR: By photocaging Tyr701 of STAT1, it is demonstrated that it is possible to photocontrol tyrosine phosphorylation and signal transduction in mammalian cells. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

Citation Index includes data from a number of different sources. If you have questions about the sources of data in the Citation Index or need a set of data which is free to re-distribute, please contact us.

Certain data included herein are derived from the Web of Science© and InCites© (2026) of Clarivate Analytics. All rights reserved. You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.