Works (6)
Updated: July 5th, 2023 15:46
2014 journal article
Genetically encoded unstrained olefins for live cell labeling with tetrazine dyes
CHEMICAL COMMUNICATIONS, 50(86), 13085–13088.
MeSH headings : Alkenes / chemistry; Amino Acids / metabolism; Amino Acyl-tRNA Synthetases / genetics; Amino Acyl-tRNA Synthetases / metabolism; Bacterial Outer Membrane Proteins / chemistry; Bacterial Outer Membrane Proteins / metabolism; Cycloaddition Reaction; Escherichia coli / metabolism; Escherichia coli Proteins / chemistry; Escherichia coli Proteins / metabolism; Fluorescein / chemistry; Fluorescent Dyes / chemistry; Green Fluorescent Proteins / chemistry; Heterocyclic Compounds, 1-Ring / chemistry; Hydrolases / chemistry; Hydrolases / metabolism; Mutation; RNA, Transfer / metabolism
TL;DR:
A number of non-canonical amino acids (NCAAs) with unstrained olefins are genetically encoded using mutant pyrrolysyl-t RNA synthetase-tRNA(Pyl)(CUA) pairs, leading to selective labeling of proteins bearing these NCAAs in live cells.
(via Semantic Scholar)
open access via europepmc.org (repository)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2014 article
Genetically encoded unstrained olefins for live cell labeling with tetrazine dyes (vol 50, pg 13085, 2014)
Lee, Y.-J., Kurra, Y., Yang, Y., Torres-Kolbus, J., Deiters, A., & Liu, W. R. (2014, November 11). CHEMICAL COMMUNICATIONS, Vol. 50, pp. 13396–13396.
TL;DR:
Genetically encoded unstrained olefins for live cell labeling with tetrazine dyes is shown to be a viable alternative for high-performance liquid chromatography of Na6(CO3)(SO4)2, Na2SO4, and Na2CO3 for cell labeling.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2014 journal article
Synthesis of Non-linear Protein Dimers through a Genetically Encoded Thiol-ene Reaction
PLOS ONE, 9(9).
MeSH headings : Alkenes / metabolism; Animals; Fluorescent Dyes / metabolism; Green Fluorescent Proteins / metabolism; Lysine / metabolism; Methanosarcina barkeri / metabolism; Muramidase / metabolism; Myoglobin / metabolism; Protein Multimerization; Sulfhydryl Compounds / metabolism
TL;DR:
The genetic encoding of a set of alkene lysines is demonstrated using the orthogonal PylRS/PylTCUA pair in Escherichia coli, showing the applicability of this approach in the formation of heterogeneous non-linear fused proteins.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2014 journal article
Two Rapid Catalyst-Free Click Reactions for In Vivo Protein Labeling of Genetically Encoded Strained Alkene/Alkyne Functionalities
BIOCONJUGATE CHEMISTRY, 25(9), 1730–1738.
MeSH headings : Alkenes / chemistry; Alkynes / chemistry; Catalysis; Click Chemistry; Cyclooctanes / chemistry; Escherichia coli Proteins / chemistry; Kinetics; Models, Molecular; Protein Conformation; Proteins / chemistry; Staining and Labeling / methods; Tyrosine / chemistry
TL;DR:
To apply these “click” reactions for site-specific protein labeling, five tyrosine derivatives that contain a norbornene, trans-cyclooctene, or cyclooctyne entity were genetically encoded into proteins in Escherichia coli using an engineered pyrrolysyl-tRNA synthetase-tRNACUAPyl pair.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2012 journal article
Genetically encoded norbornene directs site-specific cellular protein labelling via a rapid bioorthogonal reaction
NATURE CHEMISTRY, 4(4), 298–304.
MeSH headings : Amino Acids / metabolism; Amino Acyl-tRNA Synthetases / metabolism; Cell Line; Fluorescent Dyes / chemistry; Green Fluorescent Proteins / genetics; Green Fluorescent Proteins / metabolism; Humans; Lysine / analogs & derivatives; Lysine / metabolism; Norbornanes / chemistry; Proteins / genetics; Proteins / metabolism; Rhodamines / chemistry
TL;DR:
The genetic encoding of a norbornene amino acid using the pyrrolysyl tRNA synthetase/tRNA(CUA) pair in Escherichia coli and mammalian cells and the rapid bioorthogonal site-specific labelling of a protein on the mammalian cell surface is demonstrated.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2012 journal article
Photocontrol of Tyrosine Phosphorylation in Mammalian Cells via Genetic Encoding of Photocaged Tyrosine
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 134(29), 11912–11915.
MeSH headings : Amino Acyl-tRNA Synthetases / genetics; Escherichia coli / genetics; Escherichia coli / metabolism; HEK293 Cells; Humans; Light; Methanosarcina / enzymology; Methanosarcina / genetics; Mutation; Phosphorylation; Protein Engineering / methods; STAT1 Transcription Factor / genetics; STAT1 Transcription Factor / metabolism; Signal Transduction; Tyrosine / genetics; Tyrosine / metabolism
TL;DR:
By photocaging Tyr701 of STAT1, it is demonstrated that it is possible to photocontrol tyrosine phosphorylation and signal transduction in mammalian cells.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
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