2023 journal article

Evaluating the optimal tissue thickness for mass spectrometry imaging using infrared matrix-assisted laser desorption electrospray ionization

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 37(22).

By: A. Joignant n, K. Knizner n, Y. Xi n & D. Muddiman n 

TL;DR: This work defines an optimal sample thickness for IR-MALDESI-MSI and demonstrates the utility of optimizing tissue thickness for MSI platforms of comparable Z resolution. (via Semantic Scholar)
Source: Web Of Science
Added: October 30, 2023

RationaleInfrared matrix‐assisted laser desorption electrospray ionization (IR‐MALDESI) utilizes a 2970 nm mid‐IR laser to desorb samples with depth resolutions (Z) on the order of micrometers. Conventionally, 5–20 μm thick tissue sections are used to characterize different applications of the IR‐MALDESI source, but an optimal thickness has not been systematically investigated.