Ruth McMillan

author keywords: T cell receptor; Tcrb; Transcription; V(D)J recombination; Thymocytes; Double negative

MeSH headings : Acetylation; Animals; Chromatin Assembly and Disassembly; Epigenesis, Genetic; Gene Rearrangement, T-Lymphocyte; Genes, T-Cell Receptor beta; Histones / metabolism; Humans; Protein Processing, Post-Translational; Thymus Gland / enzymology; Thymus Gland / immunology; Transcription, Genetic; VDJ Recombinases / metabolism

TL;DR: Examining Tcrb assembly in developing thymocytes, the central roles of RSS elements and germline promoters as foci for epigenetic reorganization of recombinationally accessible gene segments are reviewed in light of recent findings and persistent questions. (via Semantic Scholar)

author keywords: Chromatin immunoprecipitation; Histone; Epigenetics; cad; TEA; Thymocyte; Rag

MeSH headings : Animals; Aspartate Carbamoyltransferase / genetics; Aspartate Carbamoyltransferase / metabolism; Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing) / genetics; Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing) / metabolism; Cell Line; Chromatin / genetics; Chromatin / isolation & purification; Chromatin / metabolism; Chromatin Immunoprecipitation / methods; DNA-Binding Proteins / genetics; DNA-Binding Proteins / metabolism; Dihydroorotase / genetics; Dihydroorotase / metabolism; Gene Knockdown Techniques; Histones / genetics; Histones / metabolism; Mice; Mice, Knockout; RNA Polymerase II / genetics; RNA Polymerase II / metabolism; T-Lymphocytes / metabolism

TL;DR: A streamlined ChIP is reported to efficiently carry samples from chromatin to qPCR-compatible DNA in as little as 4 h and it is shown that the protocol readily quantified histone modifications in chromatin from 10(4) Rag-deficient DN thymocytes. (via Semantic Scholar)

author keywords: T cell receptor; Transcription; V(D)J recombination; Thymocytes; Double negative; E47; GATA-3; Runx1

MeSH headings : Animals; Base Sequence; Cell Differentiation / immunology; Cell Line; Chromatin / immunology; Chromatin / metabolism; Core Binding Factor Alpha 2 Subunit / immunology; Core Binding Factor Alpha 2 Subunit / metabolism; GATA3 Transcription Factor / immunology; GATA3 Transcription Factor / metabolism; Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; Homeodomain Proteins / genetics; Homeodomain Proteins / immunology; Homeodomain Proteins / metabolism; Mice; Mice, Knockout; Molecular Sequence Data; Promoter Regions, Genetic; Receptors, Antigen, T-Cell, alpha-beta / genetics; T-Lymphocytes / immunology; T-Lymphocytes / metabolism; TCF Transcription Factors / immunology; TCF Transcription Factors / metabolism; Thymus Gland / immunology; Thymus Gland / metabolism; Transcription Factor 7-Like 1 Protein; Tumor Suppressor Protein p53 / genetics; Tumor Suppressor Protein p53 / immunology; Tumor Suppressor Protein p53 / metabolism; Upstream Stimulatory Factors / immunology; Upstream Stimulatory Factors / metabolism

TL;DR: The dependence of 5'PDbeta2 on E47 is consistent with the reported role of E proteins in post-DN1 thymocyte development and V-to-DJbeta recombination and a complex interplay among Runx1, GATA-3, and E47 transcription factors. (via Semantic Scholar)

MeSH headings : Animals; Base Sequence; Cell Differentiation / genetics; Cell Differentiation / immunology; Cell Line; Cell Line, Tumor; Gene Rearrangement, beta-Chain T-Cell Antigen Receptor / genetics; Germ-Line Mutation / immunology; Mice; Mice, Inbred C57BL; Mice, Knockout; Molecular Sequence Data; Promoter Regions, Genetic / immunology; Receptors, Antigen, T-Cell, alpha-beta / genetics; Receptors, Antigen, T-Cell, alpha-beta / metabolism; Thymus Gland / cytology; Thymus Gland / enzymology; Thymus Gland / immunology; Thymus Gland / metabolism; Transcription, Genetic / immunology; Transcriptional Activation / immunology; VDJ Recombinases / genetics

TL;DR: The unique organization and timing of Dβ2 promoter activity are consistent with a model in which promoter placement selectively regulates the rearrangement potential of D β2 during TCR β locus assembly. (via Semantic Scholar)