Clay Morrison

author keywords: Flavivirus; West Nile virus; TLR3; NS1; Innate immunity

MeSH headings : Amino Acid Substitution; DNA Mutational Analysis; HeLa Cells; Host-Pathogen Interactions; Humans; Immune Evasion; Mutant Proteins / genetics; Mutant Proteins / immunology; Toll-Like Receptor 3 / antagonists & inhibitors; Toll-Like Receptor 3 / immunology; Viral Nonstructural Proteins / genetics; Viral Nonstructural Proteins / immunology; Virus Replication; West Nile virus / genetics; West Nile virus / immunology; West Nile virus / physiology

TL;DR: A novel FACS-based screen to attempt to separate the functions of the WNV non-structural protein 1 and virus replication was developed, suggesting that the two functions are not easily separated and may be contained within overlapping domains. (via Semantic Scholar)

author keywords: West Nile virus; Flaviviridae; Nonstructural protein; Toll like receptor; Innate immunity

MeSH headings : Animals; HeLa Cells; Humans; Immunity, Innate; Luciferases / metabolism; Macrophages / immunology; Macrophages / metabolism; Mice; Mice, Inbred C57BL; Myeloid Cells / immunology; Myeloid Cells / metabolism; Poly I-C / pharmacology; Signal Transduction / immunology; Specific Pathogen-Free Organisms; Toll-Like Receptor 3 / antagonists & inhibitors; Viral Nonstructural Proteins / immunology; Viral Nonstructural Proteins / physiology; West Nile virus / immunology; West Nile virus / metabolism

TL;DR: Investigating whether the secreted form of NS1 inhibits innate immune signaling pathways in uninfected cells reduces TLR3 signaling and WNV replicon particle-mediated cytokine transcription in popliteal lymph nodes found the protein associated predominantly with macrophages and dendritic cells in the draining lymph node. (via Semantic Scholar)

author keywords: Chromatin immunoprecipitation; Histone; Epigenetics; cad; TEA; Thymocyte; Rag

MeSH headings : Animals; Aspartate Carbamoyltransferase / genetics; Aspartate Carbamoyltransferase / metabolism; Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing) / genetics; Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing) / metabolism; Cell Line; Chromatin / genetics; Chromatin / isolation & purification; Chromatin / metabolism; Chromatin Immunoprecipitation / methods; DNA-Binding Proteins / genetics; DNA-Binding Proteins / metabolism; Dihydroorotase / genetics; Dihydroorotase / metabolism; Gene Knockdown Techniques; Histones / genetics; Histones / metabolism; Mice; Mice, Knockout; RNA Polymerase II / genetics; RNA Polymerase II / metabolism; T-Lymphocytes / metabolism

TL;DR: A streamlined ChIP is reported to efficiently carry samples from chromatin to qPCR-compatible DNA in as little as 4 h and it is shown that the protocol readily quantified histone modifications in chromatin from 10(4) Rag-deficient DN thymocytes. (via Semantic Scholar)

MeSH headings : Adaptation, Biological / genetics; Amplified Fragment Length Polymorphism Analysis; Animals; Biomimetics; Body Patterning; Butterflies / physiology; Chromosome Mapping; Chromosomes, Artificial, Bacterial; Crosses, Genetic; Evolution, Molecular; Genes, Insect; Genetic Drift; Genetic Linkage; Genetic Markers; Genetic Variation; Phenotype; Predatory Behavior; Selection, Genetic; Wings, Animal / anatomy & histology; Wings, Animal / physiology

TL;DR: Comparative genetic mapping shows red wing color loci are in homologous regions of the genome of H. erato and H. melpomene and implies they evolved independently using somewhat different developmental routes, but are nonetheless regulated by the same switch locus. (via Semantic Scholar)