Works (2)

Updated: July 5th, 2023 15:58

2005 journal article

Characterization and enzymatic degradation of Sup35NM, a yeast prion-like protein

PROTEIN SCIENCE, 14(9), 2228–2235.

By: C. Chen n, K. Rojanatavorn n, A. Clark n & J. Shih n

author keywords: BSE; prion; PrPSc; Sup35NM; yeast prion; prion surrogate protein; enzymatic degradation
MeSH headings : Amino Acid Sequence; Blotting, Western; Congo Red / metabolism; Endopeptidase K / metabolism; Escherichia coli / genetics; Fluorescence; Microscopy, Electron / methods; Molecular Sequence Data; Peptide Fragments / chemistry; Peptide Fragments / isolation & purification; Peptide Fragments / metabolism; Peptide Hydrolases / metabolism; Peptide Termination Factors; Prions / chemistry; Prions / isolation & purification; Prions / metabolism; Saccharomyces cerevisiae Proteins / chemistry; Saccharomyces cerevisiae Proteins / isolation & purification; Saccharomyces cerevisiae Proteins / metabolism; Temperature; Time Factors
TL;DR: The degradation of Sup35NM aggregates by keratinase and proteinase K under various conditions was studied and compared and will be of value in understanding the mechanism and optimization of the degradation process. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2004 journal article

Increased production of Bacillus keratinase by chromosomal integration of multiple copies of the kerA gene


By: J. Wang*, K. Rojanatavorn n & J. Shih n

author keywords: keratinase; chromosomal integration; hyperproduction; Bacillus licheniformis
MeSH headings : Bacillus / enzymology; Bacillus / genetics; Bacillus subtilis / enzymology; Bacillus subtilis / genetics; Cell Proliferation; Chromosomes, Bacterial / genetics; Cloning, Molecular; Enzyme Activation; Gene Dosage; Gene Expression Regulation, Bacterial / physiology; Gene Expression Regulation, Enzymologic / physiology; Genetic Enhancement / methods; Mutagenesis, Site-Directed / genetics; Peptide Hydrolases / biosynthesis; Peptide Hydrolases / chemistry; Peptide Hydrolases / genetics; Promoter Regions, Genetic; Protein Engineering / methods; Recombinant Proteins / metabolism; Substrate Specificity
TL;DR: The strong constitutive promoter P43 not only increased the keratinase production in plasmid‐based expression in DB104 but also improved the enzyme yield of the integrants of T399D. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

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