Works (9)

Updated: July 5th, 2023 16:02

2006 journal article

Keratinase technology: from feather degradation and feed additive,to prion destruction

CAB Reviews: Perspectives in Agriculture, Veterinary Science, Nutrition and Natural Resources, 1(042), 6.

By: J. Shih & J. Wang*

Source: NC State University Libraries
Added: August 6, 2018

2006 journal article

Sup35NM-His6 aggregates: A prion-like protein useful in prion degradation studies

ENZYME AND MICROBIAL TECHNOLOGY, 40(4), 976–981.

By: J. Wang*, R. Borwornpinyo & J. Shih n

author keywords: yeast prion; Sup35NM-His6 proteases; prion degradation
TL;DR: It is suggested that Sup35NM-His6 could be a useful tool to assess the effectiveness of prion degradation for the prevention of TSE. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2005 journal article

Development of an asporogenic Bacillus licheniformis for the production of keratinase

JOURNAL OF APPLIED MICROBIOLOGY, 98(3), 761–767.

By: J. Wang*, W. Greenhut n & J. Shih n

author keywords: asporogenic Bacillus; Bacillus licheniformis; keratinase
MeSH headings : Bacillus / genetics; Bacillus / metabolism; Bacillus / physiology; Bioreactors; Genes, Bacterial; Genetic Engineering; Hot Temperature; Industrial Microbiology; Microscopy, Electron; Peptide Hydrolases / biosynthesis; Polymerase Chain Reaction; Spores, Bacterial
TL;DR: B. licheniformis PWD‐1 is a keratin‐degrading, spore‐forming bacterium isolated from a poultry waste digester and a sporulation‐deficient mutant of B. lichensiformis WBG was developed and characterized. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2004 journal article

Extracellular production of a functional soy cystatin by Bacillus subtilis

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 52(16), 5052–5056.

By: I. Kang n, J. Wang n, J. Shih n & T. Lanier n

author keywords: protease inhibitor; cloning; signal peptide; secretion; surimi
MeSH headings : Bacillus subtilis / genetics; Cloning, Molecular; Cystatins / biosynthesis; Cystatins / genetics; Cystatins / metabolism; Escherichia coli / genetics; Glucose / pharmacology; Recombinant Proteins; Soybeans / chemistry; Soybeans / genetics; Transfection
TL;DR: A recombinant Bacillus subtilis producing soy cystatin was developed by subcloning with a soy cysteine protease gene cloned in Escherichia coli and two gene fragments of signal peptides from kerA and sacB were introduced and compared for secretion efficiency of Cystatin. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2004 journal article

Increased production of Bacillus keratinase by chromosomal integration of multiple copies of the kerA gene

BIOTECHNOLOGY AND BIOENGINEERING, 87(4), 459–464.

By: J. Wang*, K. Rojanatavorn n & J. Shih n

author keywords: keratinase; chromosomal integration; hyperproduction; Bacillus licheniformis
MeSH headings : Bacillus / enzymology; Bacillus / genetics; Bacillus subtilis / enzymology; Bacillus subtilis / genetics; Cell Proliferation; Chromosomes, Bacterial / genetics; Cloning, Molecular; Enzyme Activation; Gene Dosage; Gene Expression Regulation, Bacterial / physiology; Gene Expression Regulation, Enzymologic / physiology; Genetic Enhancement / methods; Mutagenesis, Site-Directed / genetics; Peptide Hydrolases / biosynthesis; Peptide Hydrolases / chemistry; Peptide Hydrolases / genetics; Promoter Regions, Genetic; Protein Engineering / methods; Recombinant Proteins / metabolism; Substrate Specificity
TL;DR: The strong constitutive promoter P43 not only increased the keratinase production in plasmid‐based expression in DB104 but also improved the enzyme yield of the integrants of T399D. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2003 journal article

Keratinase in starter diets improves growth of broiler chicks

POULTRY SCIENCE, 82(4), 664–670.

By: N. Odetallah*, J. Wang*, J. Garlich* & J. Shih

author keywords: broiler; protease; keratinase; growth performance; starter feed
MeSH headings : Age Factors; Animal Feed; Animal Nutritional Physiological Phenomena; Animals; Body Weight / drug effects; Chickens / growth & development; Digestive System / chemistry; Dose-Response Relationship, Drug; Female; Male; Peptide Hydrolases / administration & dosage; Peptide Hydrolases / pharmacology; Random Allocation; Soybeans; Viscosity; Weight Gain / drug effects; Zea mays
TL;DR: Results of these experiments indicate that the growth of broiler chickens can be significantly improved by dietary supplementation with PWD-1 keratinase. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2003 journal article

Production and characterization of bio-immobilized keratinase in proteolysis and keratinolysis

ENZYME AND MICROBIAL TECHNOLOGY, 32(7), 812–819.

By: J. Wang n, H. Swaisgood n & J. Shih n

author keywords: keratinase; bio-immobililzation; streptavidin; fusion protein
TL;DR: Extracellular production of keratinase–streptavidin fusion protein was accomplished by the cloning of Bacillus subtilis with a transforming plasmid carrying the kerA - stp fusion gene. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2002 journal article

Bioimmobilization of keratinase using Bacillus subtilis and Escherichia coli systems

BIOTECHNOLOGY AND BIOENGINEERING, 81(4), 421–429.

By: J. Wang n, H. Swaisgood n & J. Shih n

author keywords: keratinase; immobilization; streptavidin; fusion protein
MeSH headings : Bacillus subtilis / classification; Bacillus subtilis / enzymology; Bacillus subtilis / genetics; Cells, Cultured; Enzymes, Immobilized / chemical synthesis; Enzymes, Immobilized / genetics; Enzymes, Immobilized / isolation & purification; Enzymes, Immobilized / metabolism; Escherichia coli / classification; Escherichia coli / enzymology; Gene Expression Regulation, Bacterial; Gene Expression Regulation, Enzymologic; Macromolecular Substances; Peptide Hydrolases / classification; Peptide Hydrolases / genetics; Peptide Hydrolases / isolation & purification; Peptide Hydrolases / metabolism; Protein Binding; Protein Engineering / methods; Recombinant Fusion Proteins / biosynthesis; Recombinant Fusion Proteins / genetics; Species Specificity; Streptavidin / biosynthesis; Streptavidin / classification; Streptavidin / genetics; Streptavidin / isolation & purification
TL;DR: A new genetic construct was developed to produce the keratinase-streptavidin fusion protein, so that the purification and immobilization of the fusion protein onto a biotinylated matrix can be accomplished in a single step. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

1999 journal article

Fermentation production of keratinase from Bacillus licheniformis PWD-1 and a recombinant B-subtilis FDB-29

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 22(6), 608–616.

By: J. Wang n & J. Shih n

author keywords: keratinase production; fermentation; Bacillus; recombinant strain
TL;DR: After respective optimization of fermentation conditions, keratinase production by B. licheniformis PWD-1 is approximately 40% higher than thatBy B. subtilis FDB-29, a recombinant strain, and control of pH is not necessary. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

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