Colin A. Murphree

Works (3)

Updated: July 5th, 2023 15:36

2020 journal article

A CRISPR/dCas9 toolkit for functional analysis of maize genes

Plant Methods, 16(1).

By: I. Gentzel *, C. Park *, M. Bellizzi *, G. Xiao*, K. Gadhave n, C. Murphree n, Q. Yang n, J. LaMantia* ...

Contributors: I. Gentzel *, C. Park *, M. Bellizzi *, G. Xiao*, K. Gadhave n, C. Murphree n, Q. Yang n, J. Lamantia* ...

author keywords: Maize; Protoplasts; CRISPR; Cas9; Transcription activation; Transcription suppression
topics (OpenAlex): CRISPR and Genetic Engineering; Chromosomal and Genetic Variations; Insect Resistance and Genetics
TL;DR: This study describes a vector toolkit for analyzing dCas9-mediated activation (CRISPRa) or inactivation ( CRISPRi) of gene expression in maize protoplasts and expects that this maize protoplast toolkit will streamline the analysis of gRNA candidates and facilitate genetic studies of important trait genes in this transformation-recalcitrant plant. (via Semantic Scholar)
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Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: October 26, 2020

2018 article

Metabolic and Transcriptional Profiles of Dunaliella viridis Supplemented With Ammonium Derived From Glutamine

Dums, J., Murphree, C., Vasani, N., Young, D., & Sederoff, H. (2018, August 30). Frontiers in Marine Science, Vol. 5.

By: J. Dums n, C. Murphree n, N. Vasani n, D. Young n & H. Sederoff n 

Contributors: J. Dums n, C. Murphree n, N. Vasani n, D. Young n & H. Sederoff n 

author keywords: Dunaliella; nitrogen starvation; ammonium; amino acids; glutamine; biofuel; nitrogen recycling; transcriptome
topics (OpenAlex): Algal biology and biofuel production; Marine Bivalve and Aquaculture Studies; Marine and coastal ecosystems
TL;DR: The results indicate that a continuous, amino acid derived slow release of NH4+ to algae cultures could reduce the amount of synthetic nitrogen needed for growth, but optimization is needed to balance nitrogen starvation and cell division. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, ORCID
Added: February 18, 2019

2017 article

Amino Acids Are an Ineffective Fertilizer for Dunaliella spp. Growth

Murphree, C. A., Dums, J. T., Jain, S. K., Zhao, C. S., Young, D. Y., Khoshnoodi, N., … al. (2017, May 26). Frontiers in Plant Science, Vol. 8.

By: C. Murphree n, J. Dums n, S. Jain n, C. Zhao *, D. Young n, N. Khoshnoodi*, A. Tikunov *, J. Macdonald* ...

Contributors: C. Murphree n, J. Dums n, S. Jain n, C. Zhao *, D. Young n, N. Khoshnoodi*, A. Tikunov *, J. Macdonald*, G. Pilot *, H. Sederoff n 

author keywords: Dunaliella; nitrogen recycling; lipids; biofuel; amino acids; sustainability
topics (OpenAlex): Algal biology and biofuel production; Phosphorus and nutrient management; Microbial Fuel Cells and Bioremediation
TL;DR: It is found that only four amino acids (glutamine, histidine, cysteine, and tryptophan) rescue Dunaliella spp. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

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