Colin A. Murphree

Works (3)

Updated: July 5th, 2023 15:36

2020 journal article

A CRISPR/dCas9 toolkit for functional analysis of maize genes

Plant Methods, 16(1).

By: I. Gentzel *, C. Park *, M. Bellizzi *, G. Xiao*, K. Gadhave n, C. Murphree n, Q. Yang n, J. LaMantia* ...

Contributors: I. Gentzel *, C. Park *, M. Bellizzi *, G. Xiao*, K. Gadhave n, C. Murphree n, Q. Yang n, J. Lamantia* ...

author keywords: Maize; Protoplasts; CRISPR; Cas9; Transcription activation; Transcription suppression
TL;DR: This study describes a vector toolkit for analyzing dCas9-mediated activation (CRISPRa) or inactivation ( CRISPRi) of gene expression in maize protoplasts and expects that this maize protoplast toolkit will streamline the analysis of gRNA candidates and facilitate genetic studies of important trait genes in this transformation-recalcitrant plant. (via Semantic Scholar)
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Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: October 26, 2020

2018 journal article

Metabolic and Transcriptional Profiles of Dunaliella viridis Supplemented With Ammonium Derived From Glutamine

FRONTIERS IN MARINE SCIENCE, 5(AUG).

By: J. Dums n, C. Murphree n, N. Vasani n, D. Young n & H. Sederoff n 

Contributors: J. Dums n, C. Murphree n, N. Vasani n, D. Young n & H. Sederoff n 

author keywords: Dunaliella; nitrogen starvation; ammonium; amino acids; glutamine; biofuel; nitrogen recycling; transcriptome
TL;DR: The results indicate that a continuous, amino acid derived slow release of NH4+ to algae cultures could reduce the amount of synthetic nitrogen needed for growth, but optimization is needed to balance nitrogen starvation and cell division. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, ORCID
Added: February 18, 2019

2017 journal article

Amino Acids Are an Ineffective Fertilizer for Dunaliella spp. Growth

FRONTIERS IN PLANT SCIENCE, 8.

By: C. Murphree n, J. Dums n, S. Jain n, C. Zhao *, D. Young n, N. Khoshnoodi*, A. Tikunov *, J. Macdonald* ...

Contributors: C. Murphree n, J. Dums n, S. Jain n, C. Zhao *, D. Young n, N. Khoshnoodi*, A. Tikunov *, J. Macdonald*, G. Pilot *, H. Sederoff n 

author keywords: Dunaliella; nitrogen recycling; lipids; biofuel; amino acids; sustainability
TL;DR: It is found that only four amino acids (glutamine, histidine, cysteine, and tryptophan) rescue Dunaliella spp. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

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