Karen Butler

College of Sciences

Works (4)

Updated: July 5th, 2023 14:35

2022 journal article

High-Resolution Demultiplexing (HRdm) Ion Mobility Spectrometry-Mass Spectrometry for Aspartic and Isoaspartic Acid Determination and Screening

ANALYTICAL CHEMISTRY, 94(16), 6191–6199.

By: K. Butler n, J. Dodds n, T. Flick *, I. Campuzano * & E. Baker n 

MeSH headings : Chromatography, Liquid; Ion Mobility Spectrometry / methods; Isoaspartic Acid / analysis; Mass Spectrometry / methods; Peptides
TL;DR: The rapid high-throughput HRdm DTIMS-MS method was presented to present the first application of HRdm for isoAsp and Asp assessment and demonstrate screening capabilities for isomeric peptides in complex samples, resulting in a workflow highly suitable for biopharmaceutical research needs. (via Semantic Scholar)
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Added: May 23, 2022

2021 journal article

Utilizing Ion Mobility-Mass Spectrometry to Investigate the Unfolding Pathway of Cu/Zn Superoxide Dismutase

FRONTIERS IN CHEMISTRY, 9.

By: K. Butler n, Y. Takinami, A. Rainczuk, E. Baker n  & B. Roberts *

author keywords: ion mobility spectrometry; native mass spectrometry; superoxide dismutase; SOD1; drift tube ion mobility spectrometry; trapped ion mobility spectrometry
TL;DR: The observed CCSvalues were in excellent agreement with computational CCS values predicted from the homo-dimer crystal structure, showcasing the ability to use both IMS-MS platforms to provide valuable structural information for molecular modeling of protein interactions and structural assessments. (via Semantic Scholar)
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Added: March 15, 2021

2021 article

Utilizing liquid chromatography, ion mobility spectrometry, and mass spectrometry to assess INLIGHT (TM) derivatized N-linked glycans in biological samples

Butler, K. E., Kalmar, J. G., Muddiman, D. C., & Baker, E. S. (2021, August 4). ANALYTICAL AND BIOANALYTICAL CHEMISTRY, Vol. 8.

By: K. Butler n, J. Kalmar n, D. Muddiman n  & E. Baker n 

author keywords: Glycomics; Ion mobility spectrometry; INLIGHT (TM); N-Linked glycans; LC-IMS-MS
TL;DR: The combination of IMS and the natural (NAT) and stable-isotope label (SIL) in the INLIGHT™ strategy provided additional confidence for each glycan identification due to the mobility aligned NAT- and SIL-labeled glycans and further capabilities for isomer examinations. (via Semantic Scholar)
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Added: August 16, 2021

2020 journal article

Enhanced protocol for quantitativeN-linked glycomics analysis using Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT)(TM)

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 412(27), 7569–7579.

By: J. Kalmar n, K. Butler n, E. Baker n  & D. Muddiman n 

author keywords: Glycomics; Orbitrap; Sample preparation; INLIGHT (TM); N-linked glycans; HILIC; PGC; Reversed-phase; Chromatography
MeSH headings : Chromatography, Reverse-Phase / methods; Female; Fetuins / chemistry; Glycomics / methods; Glycosylation; Humans; Male; Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase / chemistry; Polysaccharides / analysis; Tandem Mass Spectrometry / methods
TL;DR: To assess the maximum sensitivity and separability of the INLIGHT™ preparation and analysis pipeline, several critical steps were investigated and the identification of low-abundance glycans, such as (Fuc)1(Gal)2(GlcNAc)4(Man)3(NeuAc)3, was possible. (via Semantic Scholar)
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Added: September 14, 2020

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