Robert M. Grossfeld

Works (15)

Updated: April 4th, 2024 20:11

2010 article

Application of Low-Frequency Alternating Current Electric Fields Via Interdigitated Electrodes: Effects on Cellular Viability, Cytoplasmic Calcium, and Osteogenic Differentiation of Human Adipose-Derived Stem Cells

McCullen, S. D., McQuilling, J. P., Grossfeld, R. M., Lubischer, J. L., Clarke, L. I., & Loboa, E. G. (2010, March 30). Tissue Engineering Part C Methods, Vol. 16, pp. 1377–1386.

By: S. McCullen n, J. McQuilling  n, R. Grossfeld n, J. Lubischer n , L. Clarke n  & E. Loboa n

MeSH headings : Adipose Tissue / cytology; Adult Stem Cells / cytology; Adult Stem Cells / metabolism; Adult Stem Cells / physiology; Calcium / metabolism; Cell Differentiation / physiology; Cell Proliferation; Cell Survival / physiology; Cells, Cultured; Cytoplasm / metabolism; Electric Stimulation / methods; Electricity; Electrodes; Humans; Osteoblasts / metabolism; Osteoblasts / physiology; Osteogenesis / physiology
topics (OpenAlex): Planarian Biology and Electrostimulation; Electromagnetic Fields and Biological Effects; 3D Printing in Biomedical Research
TL;DR: This is the first study to evaluate the effects of sinusoidal AC electric fields on hASCs and to demonstrate that acute and chronic electric field exposure can significantly increase intracellular calcium signaling and the deposition of accreted calcium under osteogenic stimulation, respectively. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2005 article

Effect of N‐acetylaspartylglutamate (NAAG) on non‐quantal and spontaneous quantal release of acetylcholine at the neuromuscular synapse of rat

Malomouzh, A. I., Nikolsky, E. E., Lieberman, E. M., Sherman, J. A., Lubischer, J. L., Grossfeld, R. M., & Urazaev, A. K. (2005, June 10). Journal of Neurochemistry, Vol. 94, pp. 257–267.

By: A. Malomouzh *, E. Nikolsky*, E. Lieberman*, J. Sherman n, J. Lubischer n , R. Grossfeld n, A. Urazaev*

author keywords: glutamate; muscle endplate; nitric oxide; N-methyl-D-aspartate receptor
MeSH headings : Acetylcholine / metabolism; Animals; Dipeptides / pharmacology; Extracellular Space / metabolism; Hydrolysis; In Vitro Techniques; Male; Motor Neurons / metabolism; NG-Nitroarginine Methyl Ester / pharmacology; Nerve Endings / metabolism; Neuromuscular Junction / metabolism; Nitric Oxide / antagonists & inhibitors; Nitric Oxide / metabolism; Rats; Rats, Wistar; Synapses / metabolism
topics (OpenAlex): Ion channel regulation and function; Neuroscience and Neuropharmacology Research; Cardiac electrophysiology and arrhythmias
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Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2005 article

Regulation of glutamate carboxypeptidase II hydrolysis ofN‐acetylaspartylglutamate (NAAG) in crayfish nervous tissue is mediated by glial glutamate and acetylcholine receptors

Urazaev, A. K., Grossfeld, R. M., & Lieberman, E. M. (2005, March 15). Journal of Neurochemistry.

By: A. Urazaev*, R. Grossfeld n & E. Lieberman*

author keywords: glutamate carboxypeptidase II; group II metabotrophic glutamate receptor; N-acetylaspartylglutamate; nicotinic acetylcholine receptor; NMDA receptor; 2-(phosphonomethyl)-pentanedioic acid
MeSH headings : Animals; Astacoidea; Dipeptides / metabolism; Excitatory Amino Acid Antagonists / pharmacology; Glutamate Carboxypeptidase II / metabolism; Hydrolysis / drug effects; Neuroglia / drug effects; Neuroglia / metabolism; Neurons / drug effects; Neurons / metabolism; Receptors, Cholinergic / metabolism; Receptors, Metabotropic Glutamate / metabolism
topics (OpenAlex): Neuroscience and Neuropharmacology Research; Neurobiology and Insect Physiology Research; Ion channel regulation and function
TL;DR: It is concluded that NAAG released from stimulated nerve fibers activates its own hydrolysis via stimulation of GCPII activity mediated through glial mGLURII, NMDA and ACh receptors. (via Semantic Scholar)
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2002 article

Glutamine uptake and metabolism to N-acetylaspartylglutamate (NAAG) by crayfish axons and glia

Buttram, J. G., Engler, J. A., Grossfeld, R. M., Urazaev, A. K., & Lieberman, E. M. (2002, October 1). Comparative Biochemistry and Physiology Part B Biochemistry and Molecular Biology.

By: J. Buttram n, J. Engler n, R. Grossfeld n, A. Urazaev* & E. Lieberman*

author keywords: axon; glia; NAAG; glutamate; glutamine; transporter
MeSH headings : Amino Acids / pharmacology; Animals; Astacoidea / metabolism; Astacoidea / physiology; Axons / metabolism; Dipeptides / biosynthesis; Glutamine / metabolism; Neuroglia / metabolism; Neurotransmitter Agents / biosynthesis; Radioactive Tracers; Sodium / pharmacology; Tritium
topics (OpenAlex): Neuroscience and Neuropharmacology Research; Amino Acid Enzymes and Metabolism; Photoreceptor and optogenetics research
TL;DR: It is reported here, that crayfish central nerve fibers synthesize NAAG from exogenous glutamine, and the metabolism and release of radiolabeled glutamine was influenced by extracellular Na(+). (via Semantic Scholar)
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2002 article

Mechanism of NMDA receptor contribution to axon‐to‐glia signaling in the crayfish medial giant nerve fiber

Gafurov, B. S., Urazaev, A. K., Grossfeld, R. M., & Lieberman, E. M. (2002, February 25). Glia.

By: B. Gafurov*, A. Urazaev*, R. Grossfeld n & E. Lieberman*

author keywords: glutamate; N-acetylaspartylglutamate; metabotropic glutamate receptor
MeSH headings : Acetylcholine / metabolism; Animals; Astacoidea / cytology; Astacoidea / metabolism; Axons / drug effects; Axons / metabolism; Axons / ultrastructure; Calcium / metabolism; Calcium / pharmacology; Calcium Channel Blockers / pharmacology; Calcium Channels / drug effects; Calcium Channels / metabolism; Cell Communication / drug effects; Cell Communication / physiology; Cell Membrane / drug effects; Cell Membrane / metabolism; Cell Membrane / ultrastructure; Dipeptides / metabolism; Excitatory Amino Acid Agonists / pharmacology; Excitatory Amino Acid Antagonists / pharmacology; Glutamic Acid / metabolism; Membrane Potentials / drug effects; Membrane Potentials / physiology; Nervous System / cytology; Nervous System / drug effects; Nervous System / metabolism; Neural Conduction / physiology; Neuroglia / cytology; Neuroglia / drug effects; Neuroglia / metabolism; Nicotinic Antagonists / pharmacology; Receptors, N-Methyl-D-Aspartate / drug effects; Receptors, N-Methyl-D-Aspartate / metabolism; Receptors, Nicotinic / metabolism; Sodium / metabolism; Sodium / pharmacology
topics (OpenAlex): Neurobiology and Insect Physiology Research; Neuroscience and Neuropharmacology Research; Photoreceptor and optogenetics research
TL;DR: It is proposed that NMDA receptor activation by glutamate, formed from extracellular N‐acetylaspartylglutamate during nerve stimulation, contributes to glial hyperpolarization by increasing intracellular Ca2+ via opening of voltage‐sensitive Ca2- channels. (via Semantic Scholar)
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2002 article

Properties of glutaminase of crayfish CNS: implications for axon–glia signaling

Engler, J. A., Gottesman, J. M., Harkins, J. C., Urazaev, A. K., Lieberman, E. M., & Grossfeld, R. M. (2002, October 1). Neuroscience.

By: J. Engler*, J. Gottesman n, J. Harkins n, A. Urazaev*, E. Lieberman* & R. Grossfeld n

author keywords: N-acetylaspartylglutamate; glutamate; glutamine
MeSH headings : Animals; Astacoidea / enzymology; Axons / drug effects; Axons / enzymology; Central Nervous System / drug effects; Central Nervous System / enzymology; Glutaminase / antagonists & inhibitors; Glutaminase / metabolism; Neuroglia / drug effects; Neuroglia / enzymology; Signal Transduction / drug effects; Signal Transduction / physiology
topics (OpenAlex): Neuroendocrine regulation and behavior; Stress Responses and Cortisol; Neuroscience and Neuropharmacology Research
TL;DR: The results suggest that crayfish glutaminase activity is regulated by cellular changes in pH and glutamate concentration, which could impact availability of the axon-glia signaling agents glutamate and N-acetylaspartylglutamate. (via Semantic Scholar)
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2001 article

Mechanisms for clearance of released N-acetylaspartylglutamate in crayfish nerve fibers: Implications for axon–glia signaling

Urazaev, A. K., Buttram, J. G., Deen, J. P., Gafurov, B. S., Slusher, B. S., Grossfeld, R. M., & Lieberman, E. M. (2001, November 1). Neuroscience.

By: A. Urazaev*, J. Buttram*, J. Deen*, B. Gafurov*, B. Slusher *, R. Grossfeld n, E. Lieberman*

author keywords: N-acetylaspartylglutamate; glutamate; axon; glia; transport; glutamate carboxypeptidase II
MeSH headings : Animals; Astacoidea; Carboxypeptidases / metabolism; Cell Communication / physiology; Cell Membrane / metabolism; Cytoplasm / metabolism; Dipeptides / pharmacokinetics; Dizocilpine Maleate / pharmacology; Enzyme Inhibitors / pharmacology; Excitatory Amino Acid Antagonists / pharmacology; Extracellular Space / metabolism; Glutamate Carboxypeptidase II; Nerve Fibers / metabolism; Neuroglia / cytology; Neuroglia / physiology; Organophosphorus Compounds / pharmacology; Signal Transduction / physiology; Tritium
topics (OpenAlex): Neurobiology and Insect Physiology Research; Neuroscience and Neuropharmacology Research; Ion channel regulation and function
TL;DR: Results indicate that axon-to-glia signaling is terminated by NAAG hydrolysis and that the glutamate formed contributes to the glial electrical response in part via activation of NMDA receptors. (via Semantic Scholar)
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2001 article

N-Acetylaspartylglutamate (NAAG) is the probable mediator of axon-to-glia signaling in the crayfish medial giant nerve fiber

Gafurov, B., Urazaev, A. K., Grossfeld, R. M., & Lieberman, E. M. (2001, September 1). Neuroscience.

By: B. Gafurov*, A. Urazaev*, R. Grossfeld n & E. Lieberman*

author keywords: glutamate; 2-(phosphonomethyl)-pentanedioic acid; glutamate carboxypeptidase II
MeSH headings : Animals; Aspartic Acid / analogs & derivatives; Aspartic Acid / metabolism; Aspartic Acid / pharmacology; Astacoidea / cytology; Astacoidea / drug effects; Astacoidea / metabolism; Axons / drug effects; Axons / metabolism; Carboxypeptidases / antagonists & inhibitors; Carboxypeptidases / metabolism; Cell Communication / drug effects; Cell Communication / physiology; Cysteic Acid / pharmacology; Dipeptides / metabolism; Dipeptides / pharmacology; Enzyme Inhibitors / pharmacology; Excitatory Amino Acid Antagonists / pharmacology; Glutamate Carboxypeptidase II; Glutamic Acid / metabolism; Glutamic Acid / pharmacology; Membrane Potentials / drug effects; Membrane Potentials / physiology; N-Methylaspartate / pharmacology; Nervous System / cytology; Nervous System / drug effects; Nervous System / metabolism; Neuroglia / drug effects; Neuroglia / metabolism; Organophosphorus Compounds / pharmacology; Receptors, Glutamate / drug effects; Receptors, Glutamate / metabolism; Signal Transduction / drug effects; Signal Transduction / physiology; Time Factors
topics (OpenAlex): Neurobiology and Insect Physiology Research; Photoreceptor and optogenetics research; Neuroscience and Neuropharmacology Research
TL;DR: It is suggested that glutamate derived from NAAG hydrolysis appears in the periaxonal space under the conditions of these experiments and may contribute to the glial hyperpolarization. (via Semantic Scholar)
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2001 article

Synthesis and release of N-acetylaspartylglutamate (NAAG) by crayfish nerve fibers: implications for axon–glia signaling

Urazaev, A. K., Grossfeld, R. M., Fletcher, P. L., Speno, H., Gafurov, B. S., Buttram, J. G., & Lieberman, E. M. (2001, September 1). Neuroscience.

By: A. Urazaev*, R. Grossfeld n, P. Fletcher *, H. Speno*, B. Gafurov*, J. Buttram*, E. Lieberman*

author keywords: glutamate; NAAG; glutamate carboxypeptidase II; glia; axon; axon-glia interactions
MeSH headings : Animals; Aspartic Acid / metabolism; Astacoidea / cytology; Astacoidea / drug effects; Astacoidea / metabolism; Axons / drug effects; Axons / metabolism; Carbon Radioisotopes / metabolism; Carboxypeptidases / drug effects; Carboxypeptidases / metabolism; Cell Communication / drug effects; Cell Communication / physiology; Culture Media / chemistry; Culture Media / pharmacology; Cytoplasm / drug effects; Cytoplasm / metabolism; Dipeptides / biosynthesis; Dipeptides / metabolism; Dipeptides / pharmacokinetics; Electric Stimulation; Glutamate Carboxypeptidase II; Glutamic Acid / metabolism; Glutamic Acid / pharmacokinetics; Glutamine / metabolism; Nervous System / cytology; Nervous System / drug effects; Nervous System / metabolism; Neuroglia / drug effects; Neuroglia / metabolism; Organ Culture Techniques; Quisqualic Acid / pharmacology; Signal Transduction / drug effects; Signal Transduction / physiology; Tritium / metabolism; gamma-Aminobutyric Acid / metabolism
topics (OpenAlex): Nerve injury and regeneration; Pain Mechanisms and Treatments; Neuroscience and Neuropharmacology Research
TL;DR: Overall, these results suggest that glutamate is metabolized to NAAG in the giant axon and its periaxonal glia and that, upon stimulation, N AAG is released from theAxon and converted in part to glutamate by GCP II. (via Semantic Scholar)
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2000 article

Cloning and Sequence Analysis of a cDNA for an Inducible 70 kDa Heat Shock Protein (Hsp70) of the American Oyster (Crassostrea virginica)

Rathinam, A. V., Chen, T. T., & Grossfeld, R. M. (2000, January 1). DNA Sequence.

By: A. Rathinam, T. Chen* & R. Grossfeld n

MeSH headings : Amino Acid Sequence; Animals; Base Sequence; Cloning, Molecular; Consensus Sequence; Conserved Sequence; DNA, Complementary; HSP70 Heat-Shock Proteins / chemistry; HSP70 Heat-Shock Proteins / genetics; Hot Temperature; Molecular Sequence Data; Ostreidae / genetics; Sequence Analysis, DNA
topics (OpenAlex): Heat shock proteins research; thermodynamics and calorimetric analyses; Meat and Animal Product Quality
TL;DR: Northern blotting indicated that the cloned cDNA preferentially recognized an mRNA of about 2 kb that was virtually absent from visceral mass under basal conditions but greatly increased after in vivo heat shock of American and Pacific oysters, suggesting that the cDNA codes for an inducible Hsp70. (via Semantic Scholar)
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Source: NC State University Libraries
Added: August 6, 2018

2000 article

Uptake and metabolism of glutamate at non-synaptic regions of crayfish central nerve fibers: implications for axon–glia signaling

Kane, L. S., Buttram, J. G., Urazaev, A. K., Lieberman, E. M., & Grossfeld, R. M. (2000, May 1). Neuroscience.

By: L. Kane n, J. Buttram n, A. Urazaev*, E. Lieberman* & R. Grossfeld n

author keywords: glutamate; axon; glia
MeSH headings : Animals; Aspartic Acid / metabolism; Astacoidea / cytology; Astacoidea / metabolism; Attention / physiology; Axons / drug effects; Axons / metabolism; Axons / ultrastructure; Body Temperature / physiology; Excitatory Amino Acid Agonists / pharmacology; Ganglia, Invertebrate / cytology; Ganglia, Invertebrate / drug effects; Ganglia, Invertebrate / metabolism; Glutamic Acid / metabolism; Inulin / pharmacology; Neuroglia / cytology; Neuroglia / drug effects; Neuroglia / metabolism; Signal Transduction / physiology; Sodium / metabolism; Time Factors; Tritium
topics (OpenAlex): Neuroscience and Neuropharmacology Research; Biochemical effects in animals; Photoreceptor and optogenetics research
TL;DR: These studies indicate that L-glutamate is taken up primarily by the periaxonal glia of crayfish central nerve fibers by a low-affinity, saturable, Na(+)-dependent transport system and is retained by the fibers primarily in that form. (via Semantic Scholar)
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Source: Web Of Science
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1998 article

Heat-shock proteins in axoplasm: High constitutive levels and transfer of inducible isoforms from glia

Sheller, R. A., Smyers, M. E., Grossfeld, R. M., Ballinger, M. L., & Bittner, G. D. (1998, June 22). The Journal of Comparative Neurology.

By: R. Sheller*, M. Smyers*, R. Grossfeld n, M. Ballinger* & G. Bittner *

author keywords: axon; crayfish; intercellular transfer; protein transfer; stress proteins
topics (OpenAlex): Heat shock proteins research; Cellular Mechanics and Interactions; Endoplasmic Reticulum Stress and Disease
TL;DR: Data indicate that MGA axoplasm contains relatively high levels of constitutive HSP 70s and that, after heat shock, MGAAxoplasm obtains inducible HSPs of 72 kDa, 84 k da, and 87 kDa from the glial sheath. (via Semantic Scholar)
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Source: Web Of Science
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1997 journal article

Effect of Osmotic Shock on Protein Synthesis of Oyster Hemocytes In Vitro

Comparative Biochemistry and Physiology Part A: Physiology, 116(1), 43–49.

By: C. Tirard n, R. Grossfeld n, J. Levine n  & S. Kennedy-Stoskopf n

author keywords: hemocytes; osmotic; oysters; salinity; stress proteins
topics (OpenAlex): Marine Bivalve and Aquaculture Studies; Calcium Carbonate Crystallization and Inhibition; Physiological and biochemical adaptations
TL;DR: In vitro studies conducted to evaluate the effect of changes in salinity on protein synthesis of oyster hemocytes found that preferential synthesis of these proteins may represent an adaptation to preserve or restore oyster cell functions under adverse conditions. (via Semantic Scholar)
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Sources: Web Of Science, Crossref, NC State University Libraries
Added: August 6, 2018

1997 article

STRESS PROTEIN SYNTHESIS AND PEROXIDASE ACTIVITY IN A SUBMERSED AQUATIC MACROPHYTE EXPOSED TO CADMIUM

Siesko, M. M., Fleming, W. J., & Grossfeld, R. M. (1997, January 1). Environmental Toxicology and Chemistry.

By: M. Siesko n, W. Fleming* & R. Grossfeld n

author keywords: biomarkers; stress proteins; cadmium; peroxidase; pondweed
topics (OpenAlex): Environmental Toxicology and Ecotoxicology; Plant Stress Responses and Tolerance
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14. Life Below Water (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

1995 journal article

Effect of hyperthermia in vitro on stress protein synthesisand accumulation in oyster haemocytes

Fish & Shellfish Immunology, 5(1), 9–25.

By: C. Tirard*, R. Grossfeld*, J. Levine n  & S. Kennedy-Stoskopf n

author keywords: OYSTERS; CRASSOSTREA-VIRGINICA; HEMOCYTES; IMMUNE; STRESS PROTEINS; HYPERTHERMIA
topics (OpenAlex): Heat shock proteins research; Physiological and biochemical adaptations; thermodynamics and calorimetric analyses
TL;DR: The results suggest that oyster haemocytes are remarkably resilient, and that SPs may contribute to their ability to resist or repair heat-evoked damage, and could permit them to maintain immune surveillance during or immediately following serious threats to survival of these sessile ectotherms. (via Semantic Scholar)
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Sources: Web Of Science, Crossref, NC State University Libraries
Added: August 6, 2018

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