Extracellular production of a functional soy cystatin by Bacillus subtilis
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 52(16), 5052–5056.
author keywords: protease inhibitor; cloning; signal peptide; secretion; surimi
MeSH headings : Bacillus subtilis / genetics; Cloning, Molecular; Cystatins / biosynthesis; Cystatins / genetics; Cystatins / metabolism; Escherichia coli / genetics; Glucose / pharmacology; Recombinant Proteins; Soybeans / chemistry; Soybeans / genetics; Transfection
TL;DR:
A recombinant Bacillus subtilis producing soy cystatin was developed by subcloning with a soy cysteine protease gene cloned in Escherichia coli and two gene fragments of signal peptides from kerA and sacB were introduced and compared for secretion efficiency of Cystatin.
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