Kristopher Scott Moorefield

MeSH headings : Amino Acid Sequence; Animals; COS Cells; Chlorocebus aethiops; Humans; Interphase; Molecular Sequence Data; Nuclear Matrix / chemistry; Nuclear Matrix / metabolism; Protein Array Analysis; Protein Structure, Tertiary; Sequence Deletion; Sp Transcription Factors / analysis; Sp Transcription Factors / metabolism; Sp2 Transcription Factor / analysis; Sp2 Transcription Factor / genetics; Sp2 Transcription Factor / metabolism; Transfection; Zinc Fingers / genetics

TL;DR: It is concluded that Sp2 preferentially associates with the nuclear matrix and speculate that this subcellular localization plays an important role in the regulation of Sp2 function. (via Semantic Scholar)

MeSH headings : Animals; Base Sequence; COS Cells; Consensus Sequence; Cricetinae; DNA-Binding Proteins / chemistry; DNA-Binding Proteins / genetics; DNA-Binding Proteins / metabolism; Epithelial Cells / cytology; Epithelial Cells / physiology; Humans; Male; Mammals; Mice; Promoter Regions, Genetic / physiology; Prostate / cytology; Prostate-Specific Antigen / genetics; Protein Structure, Tertiary; Recombinant Fusion Proteins / genetics; Recombinant Fusion Proteins / metabolism; Sp1 Transcription Factor / chemistry; Sp1 Transcription Factor / genetics; Sp1 Transcription Factor / metabolism; Sp2 Transcription Factor; Sp3 Transcription Factor; Tetrahydrofolate Dehydrogenase / genetics; Transcription Factors / chemistry; Transcription Factors / genetics; Transcription Factors / metabolism; Transcription, Genetic / physiology

TL;DR: Sp2 is functionally distinct relative to other Sp family members and suggest that Sp2 may play a unique role in cell physiology, using chimeric molecules prepared with portions of Sp1 and Sp2 and the human prostate-specific antigen promoter. (via Semantic Scholar)

author keywords: sumoylation; trichostatin A; SP3; PML; transcriptional regulation; trans-activation; trans-repression

MeSH headings : Amino Acid Substitution; Animals; Blotting, Western; COS Cells; Cell Line; Cell Line, Tumor; Cell Nucleus / chemistry; Cell Nucleus / metabolism; Chlorocebus aethiops; DNA-Binding Proteins / chemistry; DNA-Binding Proteins / genetics; DNA-Binding Proteins / metabolism; Enzyme Inhibitors / pharmacology; Ethylmaleimide / chemistry; Humans; Hydroxamic Acids / pharmacology; Immunohistochemistry; Lysine / metabolism; Neoplasm Proteins / genetics; Neoplasm Proteins / metabolism; Nuclear Proteins / genetics; Nuclear Proteins / metabolism; Promoter Regions, Genetic / genetics; Promyelocytic Leukemia Protein; Prostate-Specific Antigen / genetics; Protein Isoforms / genetics; Protein Isoforms / metabolism; SUMO-1 Protein / genetics; SUMO-1 Protein / metabolism; Sp1 Transcription Factor / chemistry; Sp1 Transcription Factor / metabolism; Sp3 Transcription Factor; Tetrahydrofolate Dehydrogenase / genetics; Transcription Factors / chemistry; Transcription Factors / genetics; Transcription Factors / metabolism; Transcriptional Activation / drug effects; Transfection; Tumor Suppressor Proteins; Two-Hybrid System Techniques; Ubiquitin-Conjugating Enzymes / metabolism

TL;DR: It is concluded that Sp3 isoforms are sumoylated in vivo and this post-translational modification plays an important role in the regulation of Sp3-mediated transcription. (via Semantic Scholar)

MeSH headings : Amino Acids / chemistry; Animals; Binding, Competitive; Cell Line; Cell Nucleus / metabolism; DNA-Binding Proteins / genetics; DNA-Binding Proteins / metabolism; Drosophila; Gene Expression; Genes, MDR / genetics; Humans; Insecta; Mutagenesis, Site-Directed; Mutation; Plasmids / metabolism; Polymerase Chain Reaction; Promoter Regions, Genetic; Protein Binding; Protein Biosynthesis; Protein Isoforms; Protein Structure, Tertiary; Proto-Oncogene Proteins p21(ras) / genetics; Sequence Analysis, DNA; Sp3 Transcription Factor; Tetrahydrofolate Dehydrogenase / genetics; Time Factors; Transcription Factors / genetics; Transcription Factors / metabolism; Transcription, Genetic; Transcriptional Activation; Transfection; Tumor Cells, Cultured

TL;DR: Biochemical and functional analyses of mutated proteins indicate that functions encoded by the M2 carboxyl terminus, such as DNA binding activity and the capacity to form multimeric complexes, are not required or sufficient for transcriptional repression. (via Semantic Scholar)