2023 journal article
Tissue clearing and three-dimensional imaging of the whole cochlea and vestibular system from multiple large-animal models
STAR PROTOCOLS, 4(2).
author keywords: imaged cochleae using open -source software.; several mm; larger animal inner; open -source software
TL;DR:
A protocol that enables three-dimensional (3D) characterization of intact inner ears from large-animal models is presented and a pipeline to count hair cells in antibody-stained and 3D imaged cochleae using open-source software is detailed.
(via Semantic Scholar)
doi.org (publisher)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: September 5, 2023
The inner ear of humans and large animals is embedded in a thick and dense bone that makes dissection challenging. Here, we present a protocol that enables three-dimensional (3D) characterization of intact inner ears from large-animal models. We describe steps for decalcifying bone, using solvents to remove color and lipids, and imaging tissues in 3D using confocal and light sheet microscopy. We then detail a pipeline to count hair cells in antibody-stained and 3D imaged cochleae using open-source software. For complete details on the use and execution of this protocol, please refer to (Moatti et al., 2022).1.
