2023 journal article

Tissue clearing and three-dimensional imaging of the whole cochlea and vestibular system from multiple large-animal models

STAR PROTOCOLS, 4(2).

By: A. Moatti n , Y. Cai n, C. Li n, K. Popowski n , K. Cheng n, F. Ligler*, A. Greenbaum n

author keywords: imaged cochleae using open -source software.; several mm; larger animal inner; open -source software
TL;DR: A protocol that enables three-dimensional (3D) characterization of intact inner ears from large-animal models is presented and a pipeline to count hair cells in antibody-stained and 3D imaged cochleae using open-source software is detailed. (via Semantic Scholar)
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Added: September 5, 2023

The inner ear of humans and large animals is embedded in a thick and dense bone that makes dissection challenging. Here, we present a protocol that enables three-dimensional (3D) characterization of intact inner ears from large-animal models. We describe steps for decalcifying bone, using solvents to remove color and lipids, and imaging tissues in 3D using confocal and light sheet microscopy. We then detail a pipeline to count hair cells in antibody-stained and 3D imaged cochleae using open-source software. For complete details on the use and execution of this protocol, please refer to (Moatti et al., 2022).1