Elumali Sivamani

Works (8)

Updated: July 16th, 2023 21:18

2009 journal article

Sequence analysis of rice rubi3 promoter gene expression cassettes for improved transgene expression

PLANT SCIENCE, 177(6), 549–556.

By: E. Sivamani n, J. Starmer* & R. Qu n

author keywords: Exon; GUS; IME; rRNA; rubi3 promoter; Translation
TL;DR: Sequence analysis suggests that complementarity between sequences immediately 5′ of a translation initiation codon and the rice 17S rRNA may be responsible for the reduction in protein levels from constructs containing the GUS leader sequence. (via Semantic Scholar)
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15. Life on Land (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2008 journal article

Activity of the 5 ' regulatory regions of the rice polyubiquitin rubi3 gene in transgenic rice plants as analyzed by both GUS and GFP reporter genes

PLANT CELL REPORTS, 27(10), 1587–1600.

By: J. Lu n, E. Sivamani n, X. Li * & R. Qu n

author keywords: GFP; GUS; intron; rice rubi3 promoter; transgenic plant
MeSH headings : 5' Untranslated Regions / genetics; Base Pairing / genetics; Blotting, Southern; DNA, Bacterial / genetics; Gene Dosage; Gene Expression Regulation, Plant; Genes, Plant; Genes, Reporter; Glucuronidase / genetics; Green Fluorescent Proteins / genetics; Introns / genetics; Oryza / cytology; Oryza / genetics; Plant Leaves / cytology; Plant Leaves / enzymology; Plant Roots / cytology; Plant Roots / enzymology; Plants, Genetically Modified; Plasmids / genetics; Polyubiquitin / genetics; Promoter Regions, Genetic; Regulatory Sequences, Nucleic Acid / genetics
TL;DR: The rubi3 promoter should be an important addition to the arsenal of strong and constitutive promoters for monocot transformation and biotechnology. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
15. Life on Land (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2008 journal article

Gene expression enhancement mediated by the 5 ' UTR intron of the rice rubi3 gene varied remarkably among tissues in transgenic rice plants

MOLECULAR GENETICS AND GENOMICS, 279(6), 563–572.

By: J. Lu n, E. Sivamani n, K. Azhakanandam n, P. Samadder n, X. Li * & R. Qu n

author keywords: IME; intron; rubi3 gene promoter; transgenic rice; translation
MeSH headings : 5' Untranslated Regions / chemistry; Gene Expression Regulation, Plant; Genes, Plant; Genes, Reporter; Introns; Oryza / genetics; Plants, Genetically Modified / genetics; Plants, Genetically Modified / metabolism; Polyubiquitin / genetics; Promoter Regions, Genetic; Protein Biosynthesis; RNA, Messenger / metabolism; Tissue Distribution; Transformation, Genetic
TL;DR: Substantial variation in the magnitudes of intron-mediated enhancement of gene expression (IME) among tissues in rice plants is revealed and the importance of using transgenic plants for IME studies is revealed. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2008 journal article

Protamine-mediated DNA coating remarkably improves bombardment transformation efficiency in plant cells

PLANT CELL REPORTS, 28(2), 213–221.

By: E. Sivamani n, R. DeLong * & R. Qu n

author keywords: DNA coating; DNA delivery; GUS; Plant transformation; Particle bombardment; Protamine
MeSH headings : DNA / chemistry; DNA / genetics; Oryza / metabolism; Plants / genetics; Plasmids / genetics; Protamines / chemistry; Transformation, Genetic / genetics
TL;DR: It is suggested that protamine protects plasmid DNA longer than spermidine when being delivered inside the cells, probably by forming a nano-scale complex, and thus helps improve the efficiency of particle bombardment-mediated plant transformation. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2007 journal article

Expression of the bacteriophage T4 lysozyme gene in tall fescue confers resistance to gray leaf spot and brown patch diseases

TRANSGENIC RESEARCH, 17(1), 47–57.

By: S. Dong n, H. Shew  n, L. Tredway n, J. Lu n, E. Sivamani n, E. Miller n , R. Qu n

author keywords: fungal resistance; Magnaporthe grisea; Rhizoctonia solani; T4 lysozyme; tall fescue
MeSH headings : Bacteriophage T4 / enzymology; Bacteriophage T4 / genetics; Cloning, Molecular; Festuca / enzymology; Festuca / genetics; Festuca / microbiology; Gene Expression; Genes, Viral; Genetic Vectors; Magnaporthe / pathogenicity; Muramidase / genetics; Plant Diseases / genetics; Plant Diseases / microbiology; Plants, Genetically Modified; Rhizobium / genetics; Rhizoctonia / pathogenicity
TL;DR: It is demonstrated that the bacteriophage T4 lysozyme gene confers resistance to both gray leaf spot and brown patch diseases in transgenic tall fescue plants, which may have wide applications in engineered fungal disease resistance in various crops. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2007 journal article

Resistance of transgenic tall fescue to two major fungal diseases

PLANT SCIENCE, 173(5), 501–509.

By: S. Dong n, L. Tredway n, H. Shew  n, G. Wang *, E. Sivamani n & R. Qu n

author keywords: alfalfa AGLU1 gene; dermaseptin SI gene; fungal resistance; Magnaporthe grisea; Rhizoctonia solani; rice Pi9 gene
TL;DR: Of 15 T0 transgenic plants examined, 6 showed high levels of resistance to M. grisea, and 3 had enhanced resistance to R. solani, and the resistance in most of these cases was highly significant. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2006 journal article

Expression enhancement of a rice polyubiquitin gene promoter

PLANT MOLECULAR BIOLOGY, 60(2), 225–239.

By: E. Sivamani n & R. Qu n

author keywords: exon; gene expression; GUS; IME; intron; polyubiquitin; promoter; rice
MeSH headings : 5' Untranslated Regions; Base Sequence; Blotting, Western; DNA Primers; Gene Expression Regulation, Plant; Glucuronidase / genetics; Introns; Luciferases / genetics; Oryza / genetics; Polyubiquitin / genetics; Promoter Regions, Genetic; Reverse Transcriptase Polymerase Chain Reaction
TL;DR: The resultant 5′ regulatory sequence, consisting of the rubi3 promoter, 5′ UTR exon and intron, and the mutated first 9 nt coding sequence, has an activity nearly 90-fold greater than the rubu3 promoter only (without the 5′UTR intron), and 2.2-foldgreater than the maize Ubi1 gene promoter (including its 5″ UTR introns). (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2005 journal article

Selective elimination of perennial ryegrass by activation of a pro-herbicide through engineering E-coli argE gene

MOLECULAR BREEDING, 15(4), 339–347.

By: X. Chen n, W. Yang n, E. Sivamani n, A. Bruneau n, B. Wang n & R. Qu n

author keywords: argE; N-acetyl-phosphinothricin; perennial ryegrass; phosphinothricin; summer persistence; transgenics
TL;DR: This approach provides a means to selectively remove a group of transgenic plants without affecting other plants growing with them and to cause self elimination of the plants. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Source: Web Of Science
Added: August 6, 2018

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