Works (5)

Updated: July 16th, 2023 21:18

2021 journal article

Constitutive activation of nitrate reductase in tobacco alters flowering time and plant biomass

SCIENTIFIC REPORTS, 11(1).

By: J. Lu n, N. Chandrakanth n, R. Lewis n, K. Andres n, L. Bovet*, S. Goepfert*, R. Dewey n

MeSH headings : Biomass; Environment; Flowers / genetics; Gene Expression Regulation, Plant; Gene-Environment Interaction; Genetic Association Studies; Nitrate Reductase / genetics; Nitrate Reductase / metabolism; Organ Specificity / genetics; Plant Development / genetics; Tobacco / physiology; Transcriptional Activation
TL;DR: It is shown that plants expressing a constitutively active NR construct, designated 35S:S523D-NR, display an early-flowering phenotype that is also associated with a substantial reduction in plant biomass, and the mechanism by which yield was restored differed markedly between the two crosses. (via Semantic Scholar)
Source: Web Of Science
Added: March 22, 2021

2016 journal article

Expression of a constitutively active nitrate reductase variant in tobacco reduces tobacco-specific nitrosamine accumulation in cured leaves and cigarette smoke

PLANT BIOTECHNOLOGY JOURNAL, 14(7), 1500–1510.

By: J. Lu n, L. Zhang n, R. Lewis n, L. Bovet*, S. Goepfert*, A. Jack*, J. Crutchfield*, H. Ji*, R. Dewey n

author keywords: nitrate reductase; tobacco; tobacco-specific nitrosamines; burley; nitrogen-assimilation pathway; NNN; NNK
MeSH headings : Carcinogens / metabolism; Metabolic Networks and Pathways; Nitrate Reductase / genetics; Nitrates / metabolism; Nitrogen / metabolism; Nitrosamines / metabolism; Plant Leaves / genetics; Plant Leaves / metabolism; Plants, Genetically Modified / metabolism; Tobacco / genetics; Tobacco / metabolism; Tobacco Products
TL;DR: Investigation of the potential of molecular genetic strategies to lower nitrate levels in burley tobaccos by overexpressing genes encoding key enzymes of the nitrogen‐assimilation pathway found only the expression of a constitutively active nitrate reductase (NR) dramatically decreased free nitrates levels in the leaves. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2008 journal article

Expression of the bacteriophage T4 lysozyme gene in tall fescue confers resistance to gray leaf spot and brown patch diseases

TRANSGENIC RESEARCH, 17(1), 47–57.

By: S. Dong n, H. Shew n, L. Tredway n, J. Lu n, E. Sivamani n, E. Miller n, R. Qu n

author keywords: fungal resistance; Magnaporthe grisea; Rhizoctonia solani; T4 lysozyme; tall fescue
MeSH headings : Bacteriophage T4 / enzymology; Bacteriophage T4 / genetics; Cloning, Molecular; Festuca / enzymology; Festuca / genetics; Festuca / microbiology; Gene Expression; Genes, Viral; Genetic Vectors; Magnaporthe / pathogenicity; Muramidase / genetics; Plant Diseases / genetics; Plant Diseases / microbiology; Plants, Genetically Modified; Rhizobium / genetics; Rhizoctonia / pathogenicity
TL;DR: It is demonstrated that the bacteriophage T4 lysozyme gene confers resistance to both gray leaf spot and brown patch diseases in transgenic tall fescue plants, which may have wide applications in engineered fungal disease resistance in various crops. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2008 journal article

Gene expression enhancement mediated by the 5 ' UTR intron of the rice rubi3 gene varied remarkably among tissues in transgenic rice plants

MOLECULAR GENETICS AND GENOMICS, 279(6), 563–572.

By: J. Lu n, E. Sivamani n, K. Azhakanandam n, P. Samadder n, X. Li* & R. Qu n

author keywords: IME; intron; rubi3 gene promoter; transgenic rice; translation
MeSH headings : 5' Untranslated Regions / chemistry; Gene Expression Regulation, Plant; Genes, Plant; Genes, Reporter; Introns; Oryza / genetics; Plants, Genetically Modified / genetics; Plants, Genetically Modified / metabolism; Polyubiquitin / genetics; Promoter Regions, Genetic; Protein Biosynthesis; RNA, Messenger / metabolism; Tissue Distribution; Transformation, Genetic
TL;DR: Substantial variation in the magnitudes of intron-mediated enhancement of gene expression (IME) among tissues in rice plants is revealed and the importance of using transgenic plants for IME studies is revealed. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2008 journal article

Transcriptional and post-transcriptional enhancement of gene expression by the 5 ' UTR intron of rice rubi3 gene in transgenic rice cells

MOLECULAR GENETICS AND GENOMICS, 279(4), 429–439.

By: P. Samadder n, E. Sivamani n, J. Lu n, X. Li* & R. Qu n

author keywords: EJC; exon; gene expression; IME; nuclear run-on
MeSH headings : 5' Untranslated Regions; Base Sequence; DNA Primers / genetics; Gene Expression Regulation, Plant; Genes, Plant; Genes, Reporter; Introns; Oryza / genetics; Oryza / metabolism; Plant Proteins / genetics; Plants, Genetically Modified; Polyubiquitin / genetics; RNA Processing, Post-Transcriptional; RNA Splicing; RNA, Plant / genetics; RNA, Plant / metabolism; Transcription, Genetic
TL;DR: Results demonstrated transcriptional enhancement by a plant intron, but suggested that post-transcriptional event(s) be the major source of IME, implying a strong role of the intron in RNA processing and/or export. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

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