Lokesh K. Narayanan

Works (7)

Updated: July 5th, 2023 15:38

2021 journal article

Cell-Laden Nanocellulose/Chitosan-Based Bioinks for 3D Bioprinting and Enhanced Osteogenic Cell Differentiation

ACS APPLIED BIO MATERIALS, 4(3), 2342–2353.

By: P. Maturavongsadit  n, L. Narayanan n, P. Chansoria n, R. Shirwaiker n  & S. Benhabbour  n

author keywords: chitosan-based hydrogel; cellulose nanocrystal; bioink; osteogenic differentiation; bioprinting
MeSH headings : 3T3 Cells; Animals; Biocompatible Materials / chemistry; Biocompatible Materials / pharmacology; Bioprinting; Cell Differentiation / drug effects; Cells, Cultured; Cellulose / chemistry; Cellulose / pharmacology; Chitosan / chemistry; Chitosan / pharmacology; Ink; Materials Testing; Mice; Nanoparticles / chemistry; Osteogenesis / drug effects; Particle Size
TL;DR: The versatility of this CNCs-incorporated chitosan hydrogel makes it attractive as a bioink for 3D bioprinting to engineer scaffolds for bone tissue engineering and other therapeutic applications. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: April 19, 2021

2020 journal article

Effects of Autoclaving, EtOH, and UV Sterilization on the Chemical, Mechanical, Printability, and Biocompatibility Characteristics of Alginate

ACS BIOMATERIALS SCIENCE & ENGINEERING, 6(9), 5191–5201.

By: P. Chansoria n, L. Narayanan n, M. Wood n, C. Alvarado  n, A. Lin  n & R. Shirwaiker n 

author keywords: hydrogels; tissue engineering and regenerative medicine; biofabrication; printability; human adipose-derived stem cells (hASC); nuclear magnetic resonance spectroscopy
MeSH headings : Alginates; Anti-Bacterial Agents; Ethanol; Gram-Negative Bacteria; Gram-Positive Bacteria; Humans; Sterilization
TL;DR: The autoclaving process, while demonstrating broad spectrum sterility effectiveness, also resulted in most notable changes in alginate's key properties, which significantly reduced the molecular weight and polydispersity index, as determined via gel permeation chromatography, as well as the dynamic viscosity ofAlginate. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: October 12, 2020

2019 journal article

Ultrasound-assisted biofabrication and bioprinting of preferentially aligned three-dimensional cellular constructs

BIOFABRICATION, 11(3).

By: P. Chansoria  n, L. Narayanan n, K. Schuchard  n & R. Shirwaiker n 

author keywords: biofabrication; bioprinting; ultrasound; tissue engineering; 3D acoustic patterning; standing bulk acoustic waves; 3D cell manipulation
MeSH headings : Acoustics; Adipose Tissue / cytology; Alginates / pharmacology; Bioprinting / methods; Cell Survival / drug effects; Finite Element Analysis; Humans; Ink; Menisci, Tibial / drug effects; Pressure; Printing, Three-Dimensional; Stem Cells / cytology; Stem Cells / drug effects; Tissue Scaffolds / chemistry; Transducers; Ultrasonics; Viscosity
TL;DR: A new acoustophoretic three-dimensional (3D) biofabrication approach that utilizes radiation forces generated by superimposing ultrasonic bulk acoustic waves (BAW) to preferentially organize cellular arrays within single and multi-layered hydrogel constructs is described. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: May 20, 2019

2018 journal article

Label free process monitoring of 3D bioprinted engineered constructs via dielectric impedance spectroscopy

BIOFABRICATION, 10(3).

By: L. Narayanan n, T. Thompson n, R. Shirwaiker n  & B. Starly n 

author keywords: FDA PAT; Process Analytical Technology; bioprinting process parameters; biofabrication process monitoring; quality assessment
MeSH headings : Bioprinting / standards; Cell Line; Cell Survival / physiology; Cells, Cultured; Dielectric Spectroscopy / methods; Humans; Materials Testing / methods; Printing, Three-Dimensional; Stem Cells / cytology; Tissue Engineering / standards
TL;DR: The findings of this study support the use of dielectric impedance spectroscopy as a label-free and non-destructive method to characterize the critical quality attributes of bioprinted constructs. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2017 conference paper

Investigating dielectric impedance spectroscopy as a non-destructive quality assessment tool for 3D cellular constructs

Proceedings of the ASME 12th International Manufacturing Science and Engineering Conference - 2017, vol 4.

By: L. Narayanan n, T. Thompson n, A. Bhat*, B. Starly n  & R. Shirwaiker n 

Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018

2016 journal article

3D-Bioprinting of Polylactic Acid (PLA) Nanofiber–Alginate Hydrogel Bioink Containing Human Adipose-Derived Stem Cells

ACS Biomaterials Science & Engineering, 2(10), 1732–1742.

By: L. Narayanan n, P. Huebner  n, M. Fisher n, J. Spang *, B. Starly n  & R. Shirwaiker n 

Contributors: L. Narayanan n, P. Huebner  n, M. Fisher n, J. Spang *, B. Starly n  & R. Shirwaiker n 

author keywords: bioprinting; knee meniscus; alginate; nanofibers; adipose-derived stem cells; musculoskeletal soft tissue
TL;DR: This study focuses on the development and proof-of-concept assessment of a fibrous bioink composed of alginate hydrogel, polylactic acid nanofibers, and human adipose-derived stem cells for bioprinting such tissue constructs and shows that the nanofiber-reinforced bioink allowed higher levels of cell proliferation within biopprinted strands. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: August 6, 2018

2016 journal article

In vitro characterization of design and compressive properties of 3D-biofabricated/decellularized hybrid grafts for tracheal tissue engineering

JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS, 59, 572–585.

By: C. Johnson*, P. Sheshadri n, J. Ketchum *, L. Narayanan n, P. Weinberger * & R. Shirwaiker n 

Contributors: C. Johnson*, P. Sheshadri n, J. Ketchum *, L. Narayanan n, P. Weinberger * & R. Shirwaiker n 

author keywords: Trachea; Scaffolds; Tissue engineering; Decellularized ECM; Biofabrication
MeSH headings : Animals; Cartilage; Compressive Strength; Prosthesis Design; Swine; Tissue Engineering; Tissue Scaffolds; Trachea
TL;DR: The data support the possibility that future embodiments using a hybrid graft approach may reduce the need for intraluminal stenting in tracheal transplant recipients, and show that the resistance to compression offered by the hybrid grafts created using gamma radiation sterilized scaffolds was comparable to that of fresh trachesal segments. (via Semantic Scholar)
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Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

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