Sara E. Blumer-Schuette

Works (12)

Updated: July 5th, 2023 15:53

2016 journal article

A Highly Thermostable Kanamycin Resistance Marker Expands the Tool Kit for Genetic Manipulation of Caldicellulosiruptor bescii

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 82(14), 4421–4428.

By: G. Lipscomb*, J. Conway n, S. Blumer-Schuette n, R. Kelly n  & M. Adams *

MeSH headings : Firmicutes / genetics; Firmicutes / radiation effects; Genetics, Microbial / methods; Genomic Instability / radiation effects; Hot Temperature; Kanamycin Resistance; Molecular Biology / methods; Selection, Genetic
TL;DR: A selection system for C. bescii using the antibiotic kanamycin is developed and it is shown that, in combination with the biosynthetic gene marker, it can be used to efficiently delete genes in this organism. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2016 journal article

Multidomain, Surface Layer-associated Glycoside Hydrolases Contribute to Plant Polysaccharide Degradation by Caldicellulosiruptor Species

JOURNAL OF BIOLOGICAL CHEMISTRY, 291(13), 6732–6747.

By: J. Conway n, W. Pierce n, J. Le n, G. Harper n, J. Wright n, A. Tucker n, J. Zurawski n, L. Lee n, S. Blumer-Schuette n, R. Kelly n 

author keywords: biofuel; cell surface; cell surface enzyme; enzyme; glycoside hydrolase; plant cell wall; Caldicellulosiruptor; S-layer; lignocellulose
MeSH headings : Bacterial Proteins / chemistry; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Cloning, Molecular; Clostridiales / chemistry; Clostridiales / classification; Clostridiales / enzymology; Escherichia coli / genetics; Escherichia coli / metabolism; Gene Expression; Genome, Bacterial; Glucans / metabolism; Glycoside Hydrolases / chemistry; Glycoside Hydrolases / genetics; Glycoside Hydrolases / metabolism; Kinetics; Mutation; Phylogeny; Polysaccharides / metabolism; Protein Binding; Protein Engineering; Protein Structure, Tertiary; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Substrate Specificity; Wood / metabolism; Xylans / metabolism
TL;DR: The results here provide new insights into the architecture and role of SLHdomain GHs and demonstrate that hemicellulose degradation can be enhanced through non-native SLH domain GHs engineered into the genomes of Caldicelluosiruptor species. (via Semantic Scholar)
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15. Life on Land (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 journal article

Comparative Analysis of Extremely Thermophilic Caldicellulosiruptor Species Reveals Common and Unique Cellular Strategies for Plant Biomass Utilization

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 81(20), 7159–7170.

By: J. Zurawski n, J. Conway n, L. Lee n, H. Simpson  n, J. Izquierdo  n, S. Blumer-Schuette n, I. Nookaew *, M. Adams *, R. Kelly n 

MeSH headings : Bacteria / metabolism; Biomass; Cellulase / metabolism; Panicum / enzymology; Panicum / metabolism; Plants / enzymology
TL;DR: Results here show that capacity for plant biomass deconstruction varies across Caldicellulosiruptor species and depends in a complex way on GH genome inventory, substrate composition, and gene regulation. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 journal article

Discrete and Structurally Unique Proteins (Tapirins) Mediate Attachment of Extremely Thermophilic Caldicellulosiruptor Species to Cellulose

JOURNAL OF BIOLOGICAL CHEMISTRY, 290(17), 10645–10656.

By: S. Blumer-Schuette n, M. Alahuhta *, J. Conway n, L. Lee n, J. Zurawski n, R. Giannone *, R. Hettich *, V. Lunin *, M. Himmel *, R. Kelly n 

MeSH headings : Adsorption; Bacteria / genetics; Bacteria / metabolism; Bacteria / ultrastructure; Bacterial Adhesion / physiology; Bacterial Proteins / chemistry; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Binding Sites; Cellulose / metabolism; Fimbriae, Bacterial / metabolism; Genes, Bacterial; Models, Molecular; Phylogeny; Plants / microbiology; Protein Structure, Tertiary; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Recombinant Proteins / metabolism
TL;DR: Structurally unique, discrete proteins (tāpirins), not directly associated with cellulases, that mediate attachment to cellulose by species in the noncellulosomal, extremely thermophilic bacterial genus Caldicellulosiruptor are identified and define a new class of polysaccharide adhesins. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2014 review

Thermophilic lignocellulose deconstruction

[Review of ]. FEMS MICROBIOLOGY REVIEWS, 38(3), 393–448.

By: S. Blumer-Schuette n, S. Brown *, K. Sander *, E. Bayer *, I. Kataeva*, J. Zurawski n, J. Conway n, M. Adams *, R. Kelly n 

author keywords: thermophilic microorganisms; cellulosome; lignocellulose deconstruction; carbohydrate-active enzymes; systems biology; bioenergy
MeSH headings : Biofuels; Cellulosomes / genetics; Cellulosomes / metabolism; Gram-Positive Endospore-Forming Bacteria / classification; Gram-Positive Endospore-Forming Bacteria / enzymology; Gram-Positive Endospore-Forming Bacteria / genetics; Hot Temperature; Lignin / metabolism; Molecular Sequence Data; Plants / metabolism; Soil Microbiology
TL;DR: Plant-biomass-deconstructing thermophilic bacteria from the genera Clostridium and Caldicellulosiruptor, which have potential as metabolic engineering platforms for producing biofuels, are compared and contrasted from a systems biology perspective. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2013 journal article

A thermophile under pressure: Transcriptional analysis of the response of Caldicellulosiruptor saccharolyticus to different H-2 partial pressures

INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, 38(4), 1837–1849.

By: A. Bielen*, M. Verhaart*, A. VanFossen n, S. Blumer-Schuette n, A. Stams *, J. Oost *, R. Kelly n , S. Kengen *

author keywords: Caldicellulosiruptor saccharolyticus; Hydrogen partial pressure; Chemostat; Metabolic shift; Gene expression; Rex transcriptional regulator
TL;DR: Transmission data provide evidence for the involvement of the redox sensing Rex protein in gene regulation under high P H 2 cultivation conditions and differential transcription was observed for genes involved in carbon metabolism, fatty acid biosynthesis and several transport systems. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2013 journal article

Stationary Phase and Nutrient Levels Trigger Transcription of a Genomic Locus Containing a Novel Peptide (TM1316) in the Hyperthermophilic Bacterium Thermotoga maritima

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 79(21), 6637–6646.

By: A. Frock n, C. Montero n, S. Blumer-Schuette n & R. Kelly n 

MeSH headings : Carbon / metabolism; Gene Expression Regulation, Bacterial / genetics; Gene Expression Regulation, Bacterial / physiology; Genetic Loci / genetics; Microscopy, Fluorescence; Molecular Sequence Annotation / methods; Nitrogen / metabolism; Operon / genetics; Peptides / genetics; Thermotoga maritima / genetics; Thermotoga maritima / growth & development; Thermotoga maritima / metabolism
TL;DR: The onset of stationary phase in T. maritima was accompanied by significant morphological changes and upregulation of several ORFs located in the TM1298-TM1336 genome locus, which includes a putative 31-amino-acid peptide (TM1316) that was the most highly transcribed gene in the transcriptome during stationary phase. (via Semantic Scholar)
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14. Life Below Water (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2012 journal article

Caldicellulosiruptor Core and Pangenomes Reveal Determinants for Noncellulosomal Thermophilic Deconstruction of Plant Biomass

JOURNAL OF BACTERIOLOGY, 194(15), 4015–4028.

By: S. Blumer-Schuette n, R. Giannone *, J. Zurawski n, I. Ozdemir n, Q. Ma *, Y. Yin *, Y. Xu *, I. Kataeva* ...

MeSH headings : Adhesins, Bacterial / analysis; Adhesins, Bacterial / genetics; Biomass; Carbohydrate Metabolism; Cellulases / analysis; Cellulases / genetics; Cellulose / metabolism; Genetic Variation; Genome, Bacterial; Gram-Positive Bacteria / enzymology; Gram-Positive Bacteria / genetics; Metabolic Networks and Pathways / genetics; Plants / chemistry; Proteome / analysis
TL;DR: Taking into account the core genomes, pangenomes, and individual genomes, the ancestral Caldicellulosiruptor was likely cellulolytic and evolved, in some cases, into species that lost the ability to degrade crystalline cellulose while maintaining the capacity to hydrolyze amorphous cellulose and hemICEllulose. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2012 journal article

S-Layer Homology Domain Proteins Csac_0678 and Csac_2722 Are Implicated in Plant Polysaccharide Deconstruction by the Extremely Thermophilic Bacterium Caldicellulosiruptor saccharolyticus

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 78(3), 768–777.

By: I. Ozdemir n, S. Blumer-Schuette n & R. Kelly n 

MeSH headings : Enzyme Stability; Glycoside Hydrolases / chemistry; Glycoside Hydrolases / genetics; Glycoside Hydrolases / metabolism; Gram-Positive Bacteria / enzymology; Gram-Positive Bacteria / genetics; Gram-Positive Bacteria / metabolism; Hydrogen-Ion Concentration; Membrane Glycoproteins / chemistry; Membrane Glycoproteins / genetics; Membrane Glycoproteins / metabolism; Molecular Weight; Open Reading Frames; Plants / microbiology; Polysaccharides / metabolism; Protein Binding; Sequence Deletion; Temperature
TL;DR: Roles for Csac-0678 and Csac_2722 in recruitment and hydrolysis of complex polysaccharides and the deconstruction of lignocellulosic biomass are indicated and related SLH domain proteins in other Caldicellulosiruptor genomes may also be important contributors to plant biomass utilization. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2010 journal article

Phylogenetic, Microbiological, and Glycoside Hydrolase Diversities within the Extremely Thermophilic, Plant Biomass-Degrading Genus Caldicellulosiruptor

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 76(24), 8084–8092.

By: S. Blumer-Schuette n, D. Lewis n & R. Kelly n 

MeSH headings : Bacterial Proteins / analysis; Biomass; Carbohydrate Metabolism; Cluster Analysis; DNA, Bacterial / chemistry; DNA, Bacterial / genetics; DNA, Ribosomal / chemistry; DNA, Ribosomal / genetics; Electrophoresis, Gel, Two-Dimensional; Genetic Variation; Glycoside Hydrolases / genetics; Gram-Positive Bacteria / classification; Gram-Positive Bacteria / genetics; Gram-Positive Bacteria / growth & development; Gram-Positive Bacteria / metabolism; Hot Temperature; Microarray Analysis; Molecular Sequence Data; Nucleic Acid Hybridization; Phylogeny; Plants / microbiology; Proteome / analysis; RNA, Ribosomal, 16S / genetics; Sequence Analysis, DNA
TL;DR: Overall, the genus Caldicellulosiruptor appears to contain more genomic and physiological diversity than previously reported, and this argues for continued efforts to isolate new members from high-temperature terrestrial biotopes. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2008 review

Extremely thermophilic microorganisms for biomass conversion: status and prospects

[Review of ]. CURRENT OPINION IN BIOTECHNOLOGY, 19(3), 210–217.

By: S. Blumer-Schuette n, I. Kataeva*, J. Westpheling *, M. Adams * & R. Kelly n 

MeSH headings : Archaea / classification; Archaea / genetics; Archaea / metabolism; Bacteria / classification; Bacteria / genetics; Bacteria / metabolism; Bioelectric Energy Sources; Biomass; Biotechnology / trends; Carbohydrate Metabolism; Cellulase / metabolism; Cellulose / metabolism; Energy-Generating Resources; Hot Temperature; Marine Biology; Phylogeny
TL;DR: Characterization of extremely thermophilic enzymes implicated in the deconstruction of lignocellulose can be identified in genome sequences, and many more promising biocatalysts probably remain annotated as 'hypothetical proteins'. (via Semantic Scholar)
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14. Life Below Water (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

journal article

Lignocellulosic biomass deconstruction by the extremely thermophilic genus caldicellulosiruptor

Conway, J. M., Zurawski, J. V., Lee, L. L., Blumer-Schuette, S. E., & Kelly, R. M. Thermophilic Microorganisms, 91–119.

By: J. Conway, J. Zurawski, L. Lee, S. Blumer-Schuette & R. Kelly 

Source: NC State University Libraries
Added: August 6, 2018

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