Works (8)
Updated: January 20th, 2024 05:00
2023 journal article
Structural analysis and functional evaluation of the disordered ß-hexosyltransferase region from <i>Hamamotoa (Sporobolomyces) singularis</i>
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, 11.
author keywords: disorder; expression; kinetics; mutagenesis; transglycosylation; Hamamotoa singularis
TL;DR:
Functional analysis of each glycosylation site found within the C-terminal domain revealed reduced total secreted protein activity, indicating that glycosylation at all four locations is required by the host for the secretion of active enzyme and independent of the removed disordered N-terminal region.
(via Semantic Scholar)
open access via www.frontiersin.org (publisher PDF)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: December 31, 2023
2021 journal article
Safety and Modulatory Effects of Humanized Galacto-Oligosaccharides on the Gut Microbiome
FRONTIERS IN NUTRITION, 8.
author keywords: galactooligosaccharide (GOS); N-Acetyl-D-lactosamine (LacNAc); safety; human milk oligosaccharides (HMOs); LacNAc synthesis; mouse models
TL;DR:
The study demonstrated the biological synthesis of hGOS, and signaled its safety and ability to modulate the gut microbiome in vivo, promoting the growth of beneficial microorganisms, including Bifidobacterium and Akkermansia.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: April 9, 2021
2021 journal article
The pleiotropic effects of prebiotic galacto-oligosaccharides on the aging gut
MICROBIOME, 9(1).
author keywords: Gut microbiome; Prebiotics; Bifidobacterium; Intestinal permeability; Host-microbiota interactions; Diet; Antibiotics; Metagenomics; Transcriptomics; Organoids
MeSH headings : Aging / drug effects; Aging / genetics; Aging / physiology; Animals; Female; Gastrointestinal Microbiome / drug effects; Intestines / drug effects; Intestines / microbiology; Intestines / physiology; Mice; Mice, Inbred C57BL; Oligosaccharides / pharmacology; Phosphatidylinositol 3-Kinases / metabolism; Prebiotics; Proto-Oncogene Proteins c-akt / metabolism
TL;DR:
Stools from mice exhibiting variable bifidogenic response to GOS injected into colon organoids in the presence of prebiotics reproduced the response and non-response phenotypes observed in vivo suggesting that the composition and functionality of the microbiota are the main contributors to the phenotype.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: February 22, 2021
2021 article
The pleiotropic effects of prebiotic galacto-oligosaccharides on the aging gut (vol 9, 31, 2021)
Arnold, J. W., Roach, J., Fabela, S., Moorfield, E., Ding, S., Blue, E., … Azcarate-Peril, M. A. (2021, February 26). MICROBIOME, Vol. 9.
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: March 22, 2021
2015 journal article
A novel N-terminal region of the membrane beta-hexosyltransferase: its role in secretion of soluble protein by Pichia pastoris
MICROBIOLOGY-SGM, 162(1), 23–34.
Contributors: n & J. Bruno-Bárcena n
MeSH headings : Amino Acid Motifs; Amino Acid Sequence; Cell Membrane / chemistry; Cell Membrane / enzymology; Cell Membrane / genetics; Fungal Proteins / chemistry; Fungal Proteins / genetics; Fungal Proteins / metabolism; Gene Expression; Hexosyltransferases / chemistry; Hexosyltransferases / genetics; Hexosyltransferases / metabolism; Kinetics; Molecular Sequence Data; Pichia / genetics; Pichia / metabolism; Protein Transport; Yeasts / chemistry; Yeasts / enzymology; Yeasts / genetics
TL;DR:
Lead interference was demonstrated when MFα preceded the putative classical rBHT(1-22) leader, explaining the limited secretion of soluble protein by P. pastoris strains carrying combinations of the predicted structural domains.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018
2012 journal article
Heterologous Expression of a Bioactive beta-Hexosyltransferase, an Enzyme Producer of Prebiotics, from Sporobolomyces singularis
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 79(4), 1241–1249.
Contributors: n , M. Azcarate-Peril * & J. Bruno-Bárcena n
MeSH headings : Basidiomycota / enzymology; Basidiomycota / genetics; Cloning, Molecular; Enzyme Inhibitors / metabolism; Enzyme Stability; Escherichia coli / enzymology; Escherichia coli / genetics; Galactosyltransferases / chemistry; Galactosyltransferases / genetics; Galactosyltransferases / metabolism; Gene Expression; Glucose / metabolism; Hydrogen-Ion Concentration; Kinetics; Lactose / metabolism; Pichia / enzymology; Pichia / genetics; Prebiotics; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Temperature
TL;DR:
The heterologous expression of an optimized synthetically produced version of the β-hexosyltransferase gene (Bht) from Sporobolomyces singularis is described, the first report of a bioactive BHT from S. singularis that has been heterologously expressed.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018
2011 journal article
Meta-Analysis and Functional Validation of Nutritional Requirements of Solventogenic Clostridia Growing under Butanol Stress Conditions and Coutilization of D-Glucose and D-Xylose
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 77(13), 4473–4485.
Contributors: H. Heluane n, M. Dagher n & J. Bruno-Bárcena n
MeSH headings : Biofuels; Biotechnology / methods; Butanols / toxicity; Clostridium beijerinckii / drug effects; Clostridium beijerinckii / growth & development; Clostridium beijerinckii / metabolism; Culture Media / chemistry; Fermentation; Gene Expression Profiling; Gene Expression Regulation, Bacterial; Glucose / metabolism; Stress, Physiological; Xylose / metabolism
TL;DR:
Results indicated that C. beijerinckii SA-1 was able to coferment hexose/pentose sugar mixtures in the absence of a glucose repression effect, and the analysis suggests that the solvent and acid resistance mechanisms found in this strain are differentially regulated compared to strain NRRL B-527.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018
2010 article
Cell Immobilization for Production of Lactic Acid: Biofilms Do It Naturally
ADVANCES IN APPLIED MICROBIOLOGY, VOL 71, Vol. 71, pp. 113–148.
Contributors: n , A. Ragout*, F. Siñeriz * & J. Bruno-Bárcena n
MeSH headings : Biofilms / growth & development; Bioreactors; Biotechnology / methods; Cells, Immobilized; Fermentation; Industrial Microbiology / methods; Lactic Acid / biosynthesis; Lactobacillus / classification; Lactobacillus / growth & development; Lactobacillus / metabolism; Lactobacillus / physiology
TL;DR:
This chapter presents the results of a search for optimum parameters of lactic acid producing microorganisms and how they are affected by the physical, chemical, and biological variables of the process.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018
Employment
Updated: November 1st, 2021 12:02
Mammae Biosciences, Inc Raleigh, NC, US
North Carolina State University Raleigh, NC, US
Scientist
Plant and Microbial Biology
Education
Updated: December 13th, 2020 09:06
Michigan State University East Lansing, MI, US
PHD
Department of Microbial and Molecular Genetics
Funding History
Funding history based on the linked ORCID record. Updated: October 2nd, 2024 14:04
grant
August 1, 2023 - August 1, 2024
SBIR Phase I:Development of an enzymatic method to produce human milk oligosaccharides at commercial scale
NSF
Citation Index includes data from a number of different sources. If you have questions about the sources of data in the Citation Index or need a set of data which is free to re-distribute, please contact us.
Certain data included herein are derived from the Web of Science© and InCites© (2024) of Clarivate Analytics. All rights reserved. You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.