Lynette Marie Johnston

Works (7)

Updated: July 5th, 2023 15:58

2017 journal article

Microbial Load of Fresh Produce and Paired Equipment Surfaces in Packing Facilities Near the US and Mexico Border

JOURNAL OF FOOD PROTECTION, 80(4), 582–589.

By: K. Newman *, F. Bartz *, L. Johnston n, C. Moe *, L. Jaykus n & J. Leon *

author keywords: Equipment; Farm; Microbial indicator; Packing; Produce
MeSH headings : Colony Count, Microbial; Enterococcus; Escherichia coli; Food Handling; Food Microbiology; Mexico
TL;DR: Equipment surface and produce microbial loads were correlated, but correlations varied from none to high depending on the equipment surface, and the importance of packing facility sanitation to protect produce quality and safety is confirmed. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2015 journal article

Associations between Weather and Microbial Load on Fresh Produce Prior to Harvest

JOURNAL OF FOOD PROTECTION, 78(4), 849–854.

By: M. Ward, R. Dhingra*, J. Remais *, H. Chang *, L. Johnston n, L. Jaykus n, J. Leon

MeSH headings : Bacterial Load; Crops, Agricultural / growth & development; Crops, Agricultural / microbiology; Enterococcus / isolation & purification; Escherichia coli / isolation & purification; Food Microbiology; Foodborne Diseases; Rain; Temperature; United States; Weather
TL;DR: Temperature and precipitation may have a positive but complex relationship with microbial indicators, as precipitation may create moist conditions conducive to bacterial growth, spread contamination onto the field, or wash contamination off of the plant. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2008 journal article

Microbial Concentrations on Fresh Produce Are Affected by Postharvest Processing, Importation, and Season

JOURNAL OF FOOD PROTECTION, 71(12), 2389–2397.

By: E. Ailes *, J. Leon *, L. Jaykus n, L. Johnston n, H. Clayton*, S. Blanding*, D. Kleinbaum *, L. Backer*, C. Moe *

MeSH headings : Bacteria / growth & development; Bacteria / isolation & purification; Colony Count, Microbial; Commerce; Consumer Product Safety; Disease Outbreaks / prevention & control; Enterobacteriaceae / growth & development; Enterobacteriaceae / isolation & purification; Escherichia coli / growth & development; Escherichia coli / isolation & purification; Food Contamination / analysis; Food Contamination / prevention & control; Food Handling / methods; Food Handling / standards; Food Microbiology; Food Packaging / methods; Food Packaging / standards; Food Preservation / methods; Logistic Models; Predictive Value of Tests; Seasons; United States; Vegetables / microbiology; Vegetables / standards
TL;DR: A predictive, multivariate logistic regression model was developed to identify and quantify factors that were associated with detectable concentrations of E. coli contamination on produce, which included produce type, season of collection, and packing step. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2007 journal article

Design and synthesis of boronic-acid-labeled thymidine triphosphate for incorporation into DNA

Nucleic Acids Research, 35(4), 1222–1229.

By: N. Lin n, J. Yan n, Z. Huang  n, C. Altier n, M. Li  n, N. Carrasco n, M. Suyemoto n, L. Johnston n ...

MeSH headings : Aptamers, Nucleotide / chemistry; Boron Compounds / chemical synthesis; Boron Compounds / chemistry; Boron Compounds / metabolism; DNA / biosynthesis; DNA / chemistry; DNA Primers; DNA-Directed DNA Polymerase / metabolism; Polymerase Chain Reaction; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Templates, Genetic; Thymine Nucleotides / chemical synthesis; Thymine Nucleotides / chemistry; Thymine Nucleotides / metabolism
TL;DR: It is demonstrated that DNA polymerase can effectively recognize the B-TTP as the template for DNA polymerization, that allows PCR amplification of boronic acid-labeled DNA. (via Semantic Scholar)
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Source: NC State University Libraries
Added: August 6, 2018

2005 journal article

A field study of the microbiological quality of fresh produce

JOURNAL OF FOOD PROTECTION, 68(9), 1840–1847.

By: L. Johnston n, L. Jaykus n, D. Moll*, M. Martinez*, J. Anciso*, B. Mora*, C. Moe *

MeSH headings : Bacteria / growth & development; Bacteria / isolation & purification; Colony Count, Microbial; Consumer Product Safety; Food Contamination / analysis; Food Handling / methods; Food Handling / standards; Food Microbiology; Fruit / microbiology; Fruit / standards; Vegetables / microbiology; Vegetables / standards
TL;DR: It is demonstrated that each step from production to consumption may affect the microbial load of produce and reinforces government recommendations for ensuring a high-quality product. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2005 journal article

A simple method for the direct detection of Salmonella and Escherichia coli O157 : H7 from raw alfalfa sprouts and spent irrigation water using PCR

JOURNAL OF FOOD PROTECTION, 68(11), 2256–2263.

By: L. Johnston n, D. Elhanafi n, M. Drake n & L. Jaykus n

MeSH headings : Colony Count, Microbial; Consumer Product Safety; DNA, Bacterial / analysis; Escherichia coli O157 / isolation & purification; Food Microbiology; Medicago sativa / microbiology; Polymerase Chain Reaction / methods; Salmonella / isolation & purification; Sensitivity and Specificity; Water Microbiology
TL;DR: Progress was made toward development of a rapid, inexpensive, and sensitive method for the detection of sprout-associated pathogens that is relevant to current industrial practices and needs. (via Semantic Scholar)
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6. Clean Water and Sanitation (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2004 journal article

Antimicrobial resistance of Enterococcus species isolated from produce

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 70(5), 3133–3137.

By: L. Johnston n & L. Jaykus n

MeSH headings : Anti-Bacterial Agents / pharmacology; Drug Resistance, Bacterial; Enterococcus / drug effects; Enterococcus / isolation & purification; Enterococcus faecalis / drug effects; Enterococcus faecalis / isolation & purification; Enterococcus faecium / drug effects; Enterococcus faecium / isolation & purification; Food Microbiology; Fruit / microbiology; Humans; Lettuce / microbiology; Microbial Sensitivity Tests; Plants, Medicinal / microbiology; Southwestern United States
TL;DR: Enterococcus faecium strains had a much higher prevalence of resistance to ciprofloxacin, tetracycline, and nitrofurantoin than E. faecalis strains, and thirty-four percent of the isolate had multiple-drug-resistance patterns, excluding intrinsic resistance. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

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