Works (173)

Updated: April 4th, 2024 17:29

2023 article

Combined Infection Control Interventions Protect Essential Food Workers from Occupational Exposures to SARS-CoV-2 in the Agricultural Environment

Cooper, D. K., Sobolik, J. S., Kovacevic, J., Rock, C. M., Sajewski, E. T., Guest, J. L., … Leon, J. S. (2023, June 13). APPLIED AND ENVIRONMENTAL MICROBIOLOGY.

author keywords: COVID-19; quantitative microbial risk assessment (QMRA); vaccinations; nonpharmaceutical interventions (NPIs); produce industry worker
TL;DR: This model has demonstrated that the elevated daily SARS-CoV-2 infection risk experienced by indoor and outdoor produce workers can be reduced below 1% when vaccinations (optimal vaccine efficacy, 86 to 99%) are implemented with recommended infection control strategies (e.g., handwashing, surface disinfection, universal masking, physical distancing, and increased ventilation). (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: June 26, 2023

2023 journal article

Evaluation of a novel chlorine dioxide-based packaging technology to reduce human enteric virus contamination on refrigerated tomatoes and blueberries

FRONTIERS IN SUSTAINABLE FOOD SYSTEMS, 7.

By: R. Goulter n, J. Frye n, W. Kerr*, A. Richard*, M. Johnston* & L. Jaykus n

author keywords: human norovirus; hepatitis a virus; food safety; packaging; active packaging; fresh produce
Source: Web Of Science
Added: October 10, 2023

2022 journal article

Comparative Assessment of the Efficacy of Commercial Hand Sanitizers Against Human Norovirus Evaluated by an in vivo Fingerpad Method

FRONTIERS IN MICROBIOLOGY, 13.

author keywords: human norovirus; hand sanitizer; hand hygiene; fingerpad method; alcohol
TL;DR: There remains a need for more hand sanitizer formulations having greater activity against hNoV, a virus that is comparatively recalcitrant relative to other pathogens of concern in community, healthcare, and food preparation environments. (via Semantic Scholar)
Source: Web Of Science
Added: May 16, 2022

2022 journal article

Controlling risk of SARS-CoV-2 infection in essential workers of enclosed food manufacturing facilities

FOOD CONTROL, 133.

author keywords: COVID-19; Quantitative microbial risk assessment; Vaccination; Aerosol; Droplet; And fomite-mediated transmission
TL;DR: Current industry SARS-CoV-2 risk reduction strategies, particularly when bundled, provide significant protection to essential food workers. (via Semantic Scholar)
Source: Web Of Science
Added: December 20, 2021

2022 journal article

Cross-Contamination to Surfaces in Consumer Kitchens with MS2 as a Tracer Organism in Ground Turkey Patties

JOURNAL OF FOOD PROTECTION, 85(11), 1594–1603.

By: M. Kirchner n, S. Everhart n, L. Doring n, C. Smits n, J. Faircloth n, M. Duong n, R. Goulter n, L. Goodson n ...

author keywords: Consumer behavior; Cross-contamination; Foodborne illness; Kitchen
MeSH headings : Animals; Food Handling; Foodborne Diseases; Turkeys; Lettuce; Food Microbiology; Food Contamination / analysis
TL;DR: The high level of MS2 on spice containers drove the significant differences between surfaces, suggesting the significance of spice containers as a vehicle for cross-contamination, despite the absence of previous reports to this effect. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: January 30, 2023

2022 journal article

Decontamination of SARS-CoV-2 from cold-chain food packaging provides no marginal benefit in risk reduction to food workers

FOOD CONTROL, 136.

author keywords: COVID-19; Quantitative microbial risk assessment; Cold-chain fomite-mediated transmission; Plastic packaging
TL;DR: Given that standard infection control interventions reduced risks well below 1 × 10−4 (World Health Organization water quality risk thresholds), additional packaging decontamination suggest no marginal benefit in risk reduction. (via Semantic Scholar)
Source: Web Of Science
Added: May 16, 2022

2022 journal article

Efficacy and Mechanisms of Copper Ion-Catalyzed Inactivation of Human Norovirus br

ACS INFECTIOUS DISEASES, 8(4), 855–864.

By: B. Mertens n, M. Moore n, L. Jaykus n & O. Velev n

author keywords: norovirus; viral inactivation; antiviral; copper; Tulane virus; disinfection
MeSH headings : Alloys / pharmacology; Capsid Proteins; Catalysis; Copper / pharmacology; Ions; Norovirus / drug effects; Norovirus / physiology; Virus Inactivation
TL;DR: The combined data demonstrate that stabilized Cu(I) ion solutions show promise as highly effective noroviral disinfectants in solution that can potentially be utilized at low concentrations for inactivation of human noroviruses. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: May 16, 2022

2022 article

Efficacy of an alcohol-based surface disinfectant formulation against human norovirus

Escudero-Abarca, B. I., Goulter, R. M., Bradshaw, J., Faircloth, J., Leslie, R. A., Manuel, C. S., … Jaykus, L.-A. (2022, February 19). JOURNAL OF APPLIED MICROBIOLOGY.

author keywords: food safety; food virology; human intestinal enteroids; human norovirus; surface disinfection; surface sanitizing; Tulane virus
MeSH headings : Disinfectants / pharmacology; Disinfection / methods; Ethanol; Humans; Norovirus / genetics; Sodium Hypochlorite / pharmacology; Soil; Stainless Steel
TL;DR: To evaluate the anti‐noroviral efficacy of PURELL® surface sanitizer and disinfectant spray (PSS, an alcohol‐based formulation) using human norovirus GII.4 Sydney and its cultivable surrogate, Tulane virus (TuV, infectivity assay), compared to sodium hypochlorite (NaOCl) solutions. (via Semantic Scholar)
Source: Web Of Science
Added: March 14, 2022

2022 journal article

Observational Study of the Impact of a Food Safety Intervention on Consumer Poultry Washing

JOURNAL OF FOOD PROTECTION, 85(4), 615–625.

By: E. Shumaker n, M. Kirchner n, S. Cates*, L. Shelley n, R. Goulter n, L. Goodson n, C. Bernstein, A. Lavallee*, L. Jaykus n, B. Chapman n

author keywords: Consumers; Meal preparation; Observation; Poultry washing; Test kitchen
MeSH headings : Animals; Escherichia coli; Food Contamination / analysis; Food Contamination / prevention & control; Food Handling / methods; Food Microbiology; Food Safety; Hand; Humans; Poultry
TL;DR: The need to change the frame of "don't wash your poultry" messaging to instead focus on preventing contamination of sinks and continuing to emphasize the importance of handwashing and cleaning and sanitizing surfaces is demonstrated. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: September 19, 2022

2022 journal article

Survey evaluation of dog owners' feeding practices and dog bowls' hygiene assessment in domestic settings

PLOS ONE, 17(4).

By: E. Luisana n, K. Saker n, L. Jaykus n & C. Getty n

MeSH headings : Animals; Dogs; Food Contamination / prevention & control; Food Handling; Humans; Hygiene; Pets; Surveys and Questionnaires; Water
TL;DR: A need for pet food handling and dish hygiene guideline education to minimize bacterial contamination of dishes, especially for high-risk populations is suggested. (via Semantic Scholar)
Source: Web Of Science
Added: July 5, 2022

2022 article

The Efficacy of Commercial Surface Sanitizers against Norovirus on Formica Surfaces with and without Inclusion of a Wiping Step

Faircloth, J., Goulter, R. M., Manuel, C. S., Arbogast, J. W., Escudero-Abarca, B., & Jaykus, L.-A. (2022, August 25). APPLIED AND ENVIRONMENTAL MICROBIOLOGY.

author keywords: human norovirus; Tulane virus; sanitize; disinfect; wiping
MeSH headings : Animals; Calicivirus, Feline; Cats; Disinfectants / pharmacology; Ethanol; Gastroenteritis; Humans; Norovirus / genetics; Quaternary Ammonium Compounds; Virus Inactivation; Water
TL;DR: Assessment of the anti-hNoV efficacy of various surface sanitizer chemistries, as applied to a laminate material commonly used for restaurant tabletops, using standard surface assays and a newly developed wiping protocol highlights the importance of wiping in the process of sanitization. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: Web Of Science
Added: September 6, 2022

2021 journal article

Analysis of Bacterial Communities by 16S rRNA Gene Sequencing in a Melon-Producing Agro-environment

MICROBIAL ECOLOGY, 82(3), 613–622.

author keywords: Cantaloupe farms; 16 s RNA; Sequencing; Bacterial population
MeSH headings : Bacteria / genetics; Cucurbitaceae; Genes, rRNA; RNA, Ribosomal, 16S / genetics; Salmonella
TL;DR: Information is provided on the cantaloupe production microbiome, which can inform future research into critical food safety issues such as antimicrobial resistance, virulence, and genomic epidemiology. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: March 8, 2021

2021 journal article

Comparison of a one-step real-time RT-PCR and a nested real-time RT-PCR for a genogroup II norovirus reveals differences in sensitivity depending upon assay design and visualization

PLOS ONE, 16(4).

By: C. Manuel n, C. Suther*, M. Moore n & L. Jaykus n

MeSH headings : Caliciviridae Infections / diagnosis; Caliciviridae Infections / genetics; Caliciviridae Infections / metabolism; Feces / virology; Female; Genome, Viral; Humans; Male; Norovirus / genetics; RNA, Viral / genetics; RNA, Viral / metabolism; Real-Time Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction
TL;DR: A controlled comparison of two published NoV detection assays shows the complexities in designing and interpreting molecular techniques having a sufficient detection limit to detect low levels of viruses that might be anticipated in contaminated food and environmental samples. (via Semantic Scholar)
Source: Web Of Science
Added: June 10, 2021

2021 review

Cross-Contamination on Atypical Surfaces and Venues in Food Service Environments

[Review of ]. JOURNAL OF FOOD PROTECTION, 84(8), 1239–1251.

By: M. Kirchner n, R. Goulter n, B. Chapman n, J. Clayton & L. Jaykus n

author keywords: Atypical surface; Atypical venue; Cross-contamination; Food service; Pathogens; Retail
MeSH headings : Food Contamination / analysis; Food Handling; Food Microbiology; Food Safety; Food Services; Foodborne Diseases; Hand; Hand Disinfection; Humans
TL;DR: This review highlights key surfaces and venues that have the potential to be cross-contaminated and have been underestimated or not fully investigated and knowledge gaps indicate where further work is needed to fully understand the role of these surfaces and venue inCross-contamination and how it can be prevented. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: July 26, 2021

2021 journal article

Generation of Nucleic Acid Aptamer Candidates against a Novel Calicivirus Protein Target

VIRUSES-BASEL, 13(9).

By: J. Faircloth n, M. Moore n, S. Stoufer*, M. Kim* & L. Jaykus n

author keywords: norovirus; aptamer; VPg; detection; Norwalk virus; Tulane virus; infectivity; therapeutics
MeSH headings : Aptamers, Nucleotide / genetics; Caliciviridae / genetics; DNA, Single-Stranded / genetics; DNA, Single-Stranded / metabolism; Genome, Viral; Humans; Nucleic Acids / genetics; Nucleic Acids / metabolism; SELEX Aptamer Technique / methods; Viral Proteins / genetics; Viral Proteins / metabolism
TL;DR: A number of aptamer candidates found to be broadly reactive and specific for in vitro-expressed VPgs across genus that could be used for future application in detection or therapeutics are reported. (via Semantic Scholar)
Source: Web Of Science
Added: October 12, 2021

2021 journal article

Harnessing Whole Genome Sequence Data for Facility-Specific Signatures for Listeria monocytogenes: A Case Study With Turkey Processing Plants in the United States

FRONTIERS IN SUSTAINABLE FOOD SYSTEMS, 5.

By: P. Brown n, Y. Chen*, R. Siletzky n, C. Parsons n, L. Jaykus n, J. Eifert*, E. Ryser*, C. Logue* ...

Contributors: P. Brown n, P. Brown* & P. Brown

Ed(s): P. Brown n

author keywords: Listeria monocytogenes; inlA; whole genome sequencing; turkey processing plant; persistence; plant-specific signatures; cgMLST; wgMLST
TL;DR: Deploying whole genome sequencing data for intra-sample strain analysis allows for a more complete environmental surveillance of L. monocytogenes in food processing facilities, reducing the risk of failing to detect strains that may be clinically relevant and potentially novel. (via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: November 15, 2021

2021 journal article

Potassium chloride-based replacers: modeling effects on sodium and potassium intakes of the US population with cross-sectional data from NHANES 2015-2016 and 2009-2010

AMERICAN JOURNAL OF CLINICAL NUTRITION, 114(1), 220–230.

author keywords: dietary modeling; potassium chloride; reformulation; salt; sodium chloride replacer; sodium reduction
MeSH headings : Cross-Sectional Studies; Food; Humans; Models, Biological; Nutrition Surveys; Potassium Chloride / administration & dosage; Potassium, Dietary / administration & dosage; Sodium, Dietary / administration & dosage; United States
TL;DR: The F&P replacement of NaCl with KCl in top-ranking sources of dietary sodium modeled in this study can result in decreased sodium to a level consistent with the short-term intake goal targeted by the FDA of 3000 mg/d, with the mean potassium intake remaining in the range recommended for the apparently healthy population. (via Semantic Scholar)
Source: Web Of Science
Added: August 2, 2021

2021 journal article

Salmonella enterica colonization and fitness in pre-harvest cantaloupe production

FOOD MICROBIOLOGY, 93.

By: K. Burris*, O. Simmons, H. Webb, R. Moore, L. Jaykus*, J. Zheng, E. Reed, C. Ferreira, E. Brown, R. Bell

author keywords: Salmonella; Cantaloupe; Soil; Food safety; Internalization; Blossom
MeSH headings : Cucumis melo / microbiology; Food Contamination / analysis; Food Handling; Food Microbiology; Food Safety; Foodborne Diseases; Fruit / microbiology; Salmonella; Salmonella enterica / genetics; Salmonella enterica / growth & development; Serotyping; Soil; Soil Microbiology; Temperature
TL;DR: It is demonstrated that contaminated soil and blossoms can lead to Salmonella internalization into the plant or fruit at a relatively high frequency. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: October 5, 2020

2021 journal article

The Cantaloupe Farm Environment Has a Diverse Genetic Pool of Antibiotic-Resistance and Virulence Genes

FOODBORNE PATHOGENS AND DISEASE, 18(7), 469–476.

author keywords: antimicrobial resistance; cantaloupe farms; virulence genes; Salmonella; E. coli O157:H7
MeSH headings : Anti-Bacterial Agents / pharmacology; Cucumis melo / microbiology; Drug Resistance, Bacterial; Drug Resistance, Microbial; Environmental Microbiology; Farms; Food Contamination / analysis; Food Handling / methods; Food Microbiology; Mexico; Microbial Sensitivity Tests; Salmonella / genetics; Salmonella / isolation & purification; Salmonella / pathogenicity; Virulence
TL;DR: A diverse pool of antibiotic-resistance and VGs in cantaloupe production is demonstrated, and Salmonella genes invA and spiA were the most abundant among VGs. (via Semantic Scholar)
Source: Web Of Science
Added: May 17, 2021

2020 journal article

An Observational Study of Thermometer Use by Consumers When Preparing Ground Turkey Patties

JOURNAL OF FOOD PROTECTION, 83(7), 1167–1174.

By: M. Duong n, E. Shumaker*, S. Cates*, L. Shelley n, L. Goodson n, C. Bernstein*, A. Lavallee*, M. Kirchner n ...

MeSH headings : Animals; Cooking; Food Handling; Food Safety; Humans; North Carolina; Thermometers
TL;DR: This study demonstrates the importance of timing and framing of a behavioral intervention for thermometer use, and highlights considerations for the development of additional messages (e.g., proper insertion). (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 24, 2020

2020 journal article

Both Handwashing and an Alcohol-Based Hand Sanitizer Intervention Reduce Soil and Microbial Contamination on Farmworker Hands during Harvest, but Produce Type Matters

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 86(18).

By: J. Prince-Guerra*, M. Nace*, R. Lyles*, A. Aceituno*, F. Bartz*, J. Arbogast*, J. Gentry-Shields n, L. Jaykus n ...

author keywords: bacterial indicators; environmental microbiology; food microbiology; food-borne pathogens; fruit; hand hygiene; produce; vegetables
MeSH headings : Bacterial Load / drug effects; Capsicum / growth & development; Crop Production; Crops, Agricultural / classification; Cucumis melo / growth & development; Ethanol / chemistry; Farmers; Hand / microbiology; Hand Disinfection / instrumentation; Hand Sanitizers / administration & dosage; Hand Sanitizers / chemistry; Humans; Solanum lycopersicum / growth & development; Mexico; Soil
TL;DR: It is found that a two-step ABHS intervention was similar to handwashing with soap and water at reducing bacteria on farmworker hands, and produce commodity type should be considered when developing hand hygiene interventions on farms. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: Web Of Science
Added: September 28, 2020

2020 journal article

Colonization and Internalization of Salmonella enterica and Its Prevalence in Cucumber Plants

Frontiers in Microbiology, 11.

By: K. Burris n, O. Simmons n, H. Webb n, L. Deese n, R. Moore n, L. Jaykus n, J. Zheng*, E. Reed* ...

author keywords: Salmonella; cucumber; fitness; internalization; blossom
TL;DR: Investigation of routes of colonization and internalization of S. enterica into cucumber plants by various routes of contamination identified blossoms as an important route by which Salmonella internalized at a high percentage into cucumbers, and S. Poona, the same strain isolated from the 2015 outbreak of cucumbers imported from Mexico, was shown to be well-adapted to the blossom niche. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: July 13, 2020

2020 journal article

Comparison of estimated norovirus infection risk reductions for a single fomite contact scenario with residual and nonresidual hand sanitizers

AMERICAN JOURNAL OF INFECTION CONTROL, 48(5), 538–544.

author keywords: Norovirus; Hand sanitizer; Risk reduction; Risk target
MeSH headings : Adult; Caliciviridae Infections / prevention & control; Caliciviridae Infections / virology; Ethanol / administration & dosage; Female; Fomites / virology; Hand / virology; Hand Disinfection / methods; Hand Sanitizers / administration & dosage; Hand Sanitizers / chemistry; Humans; Male; Norovirus / drug effects; Risk Reduction Behavior; Time Factors; Virus Inactivation / drug effects
TL;DR: Residual antinoroviral hand sanitizers may reduce infection risks for up to 4 hours, and log10 virus and infection risk reductions were consistently greater for the residualHand sanitizer under all scenarios. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: June 15, 2020

2020 journal article

Efficacy of alcohol-based hand sanitizers against human norovirus using RNase-RT-qPCR with validation by human intestinal enteroid replication

LETTERS IN APPLIED MICROBIOLOGY, 71(6), 605–610.

By: B. Escudero-Abarca n, R. Goulter n, J. Arbogast*, R. Leslie*, K. Green* & L. Jaykus n

author keywords: disinfection; food safety; human intestinal enteroids; human norovirus; RT-qPCR
MeSH headings : Caliciviridae Infections / virology; Drug Evaluation, Preclinical; Ethanol / pharmacology; Hand Sanitizers / pharmacology; Humans; Intestines / virology; Norovirus / drug effects; Norovirus / genetics; Norovirus / growth & development; Norovirus / physiology; Real-Time Polymerase Chain Reaction / instrumentation; Ribonucleases / metabolism; Virus Replication / drug effects
TL;DR: The purpose of this study was to evaluate the anti‐HuNoV efficacy of two alcohol‐based commercial hand sanitizers and 60% ethanol by suspension assay using RNase‐RT‐qPCR, with subsequent validation of efficacy by HuNoV cultivation using the HIE model. (via Semantic Scholar)
Source: Web Of Science
Added: October 19, 2020

2020 journal article

In-House Validation of a Rinse-Membrane Filtration Method for Processing Fresh Produce Samples for Downstream Cultural Detection of Salmonella, Escherichia coli O157:H7, and Listeria

JOURNAL OF FOOD PROTECTION, 83(9), 1592–1597.

author keywords: Escherichia coli O157:H7; Listeria; Membrane filtration; Pathogen detection; Salmonella
MeSH headings : Colony Count, Microbial; Escherichia coli O157; Food Microbiology; Listeria; Listeria monocytogenes; Salmonella
TL;DR: Overall, the RMFM performed similar or better than the homogenization and sponge surface rubbing methods, and is a good alternative for processing large numbers of produce samples for bacterial pathogen detection. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: September 28, 2020

2020 article

The impact of COVID-19 on food systems, safety, and security-a symposium report

Cable, J., Jaykus, L.-A., Hoelzer, K., Newton, J., & Torero, M. (2021, January). ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, Vol. 1484, pp. 3–8.

By: J. Cable, L. Jaykus n, K. Hoelzer*, J. Newton* & M. Torero*

author keywords: COVID-19; SARS-CoV-2; food safety; food surveillance; food security
MeSH headings : COVID-19 / economics; COVID-19 / epidemiology; Congresses as Topic; Food Safety; Food Services / economics; Food Services / legislation & jurisprudence; Food Services / organization & administration; Food Services / standards; Humans; Pandemics / economics; SARS-CoV-2
TL;DR: The recent SARS‐CoV‐2 global pandemic has affected multiple aspects of the food systems, from federal governments’ decisions to limit food exports, to the ability of government agencies to inspect food and facilities to the able of consumers to dine at restaurants. (via Semantic Scholar)
Source: Web Of Science
Added: September 14, 2020

2019 book

Science Breakthroughs to Advance Food and Agricultural Research by 2030

In SCIENCE BREAKTHROUGHS TO ADVANCE FOOD AND AGRICULTURAL RESEARCH BY 2030 (pp. 1–228).

By: D. Allison, C. Brown, L. Goddard, M. Guerinot, J. Jansson, L. Jaykus, H. Jensen, R. Khosla ...

UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: November 24, 2020

2018 personal communication

Disinfectant testing against human norovirus surrogates-What infection preventionists need to know

Clayton, J. S., Bolinger, H. K., & Jaykus, L.-A. (2018, November).

By: J. Clayton, H. Bolinger* & L. Jaykus n

MeSH headings : Caliciviridae Infections / prevention & control; Calicivirus, Feline / drug effects; Disinfectants / pharmacology; Humans; Norovirus / drug effects; United States; United States Environmental Protection Agency; Virus Inactivation / drug effects
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: January 21, 2019

2018 journal article

Phylogroups, pathotypes, biofilm formation and antimicrobial resistance of Escherichia coli isolates in farms and packing facilities of tomato, jalapeno pepper and cantaloupe from Northern Mexico

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 290, 96–104.

By: H. Andrea Corzo-Ariyama*, A. Garcia-Heredia*, N. Heredia*, S. Garcia*, J. Leon*, L. Jaykus n, L. Solis-Soto*

author keywords: Phylogroup; Pathotypes; Biofilm; Antibiotic resistance; E. coli
MeSH headings : Anti-Bacterial Agents / pharmacology; Biofilms / drug effects; Capsicum / microbiology; Cucumis melo / microbiology; Drug Resistance, Bacterial / drug effects; Environmental Microbiology; Escherichia coli / classification; Escherichia coli / drug effects; Escherichia coli / genetics; Escherichia coli / pathogenicity; Farmers; Farms; Humans; Solanum lycopersicum / microbiology; Manufacturing and Industrial Facilities; Mexico; Phylogeny
TL;DR: Analysis of naturally occurring E. coli strains isolated from the jalapeño pepper, tomato and cantaloupe farm environments, in Northeast Mexico, suggests potential health risks for consumers and identifies antibiotic resistant E.coli strains that may contain gene markers for pathogenicity and which can form biofilms. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: February 11, 2019

2018 journal article

Predicting human norovirus infectivity - Recent advances and continued challenges

Food Microbiology, 76, 337–345.

By: C. Manuel n, M. Moore* & L. Jaykus n

author keywords: Norovirus; Infectivity; Cell culture; Histo-blood group antigen; Aptamer
MeSH headings : Animals; Caliciviridae Infections / virology; Cell Culture Techniques / methods; Disease Models, Animal; Gastroenteritis / virology; Humans; In Vitro Techniques / methods; Mice; Norovirus / growth & development; Norovirus / isolation & purification; Norovirus / physiology; Virology / methods
TL;DR: The purpose of this review is to present the multiple recent techniques used to assess human norovirus infectivity, including recently described human Norovirus in vitro cultivation systems, cultivable surrogate viruses, animal models, and alternative molecular techniques, and discuss their advantages and disadvantages in the context of determining human norova infectivity. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Crossref
Added: February 24, 2020

2018 journal article

Use of DNA aptamer for sandwich type detection of Listeria monocytogenes

ANALYTICAL BIOCHEMISTRY, 557, 27–33.

MeSH headings : Aptamers, Nucleotide / chemistry; Aptamers, Nucleotide / genetics; Enzyme-Linked Immunosorbent Assay; Listeria monocytogenes / cytology; Listeria monocytogenes / isolation & purification
TL;DR: A single stranded (ss) DNA aptamer, specific to members of Listeria genus, was used to develop a two-site binding sandwich assay for capture and detection of L. monocytogenes. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: NC State University Libraries
Added: October 19, 2018

2018 review

Virus-Bacteria Interactions: Implications and Potential for the Applied and Agricultural Sciences

[Review of ]. VIRUSES-BASEL, 10(2).

By: M. Moore* & L. Jaykus n

author keywords: virus-bacteria interaction; agricultural sciences; translational medicine; foodborne pathogens; agronomy; influenza; norovirus
MeSH headings : Agriculture; Animals; Bacterial Physiological Phenomena; Coinfection; Foodborne Diseases / microbiology; Foodborne Diseases / virology; Humans; Microbial Interactions; Virus Physiological Phenomena; Waterborne Diseases / microbiology; Waterborne Diseases / virology
TL;DR: Eukaryotic virus–bacteria interactions have importance and deserve more investigation, as numerous potential consequences and applications arising from their discovery are relevant to the applied sciences. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2017 review

A Systematic Review of the Use of Social Media for Food Safety Risk Communication

[Review of ]. JOURNAL OF FOOD PROTECTION, 80(9), 1537–1549.

By: K. Overbey n, L. Jaykus n & B. Chapman n

Contributors: K. Overbey n, L. Jaykus n & B. Chapman n

author keywords: Foodborne illness; Food safety; Infectious disease; Social media
MeSH headings : Consumer Product Safety; Food Safety; Health Communication; Humans; Risk; Social Media
TL;DR: The wide reach, immediacy, and information gathering capacities of social media were frequently cited benefits, and suggestions for social media best practices were inconsistent among studies, and study designs were highly variable. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2017 journal article

A plate-based histo-blood group antigen binding assay for evaluation of human norovirus receptor binding ability

ANALYTICAL BIOCHEMISTRY, 533, 56–59.

By: M. Moore n & L. Jaykus n

author keywords: Norovirus; Disinfection; Capsid functionality; Histo-blood group antigens; Receptor binding assay
MeSH headings : Amino Acid Sequence / genetics; Blood Group Antigens / immunology; Blood Group Antigens / isolation & purification; Capsid Proteins / blood; Gastroenteritis / diagnosis; Gastroenteritis / virology; Genotype; Humans; Norovirus / genetics; Norovirus / immunology; Norovirus / isolation & purification; Norovirus / pathogenicity; Protein Binding
TL;DR: A streamlined version of a receptor-binding assay with minimal time-to-result while maintaining accuracy and high throughput is presented, validating assay performance for physical and chemical inactivation treatments of a norovirus GII. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2017 journal article

Contamination of Fresh Produce by Microbial Indicators on Farms and in Packing Facilities: Elucidation of Environmental Routes

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 83(11).

By: F. Bartz*, J. Lickness*, N. Heredia*, A. Aceituno*, K. Newman*, D. Hodge*, L. Jaykus n, S. Garcia*, J. Leon*

author keywords: environmental microbiology; food-borne pathogens; produce
MeSH headings : Bacteria / genetics; Bacteria / isolation & purification; Farms; Food Contamination / analysis; Food Microbiology / instrumentation; Food Packaging / instrumentation; Hand / microbiology; Humans; Mexico; Workforce
TL;DR: The relationship between microbial contamination on hands, soil, and water and contamination on fresh produce in the agricultural production environment can be used to support the design of targeted interventions to prevent or reduce microbial contamination of fresh produce with associated reductions in foodborne illness. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2017 journal article

Development of a Recombinase Polymerase Amplification Assay for Detection of Epidemic Human Noroviruses

SCIENTIFIC REPORTS, 7.

By: M. Moore n & L. Jaykus n

MeSH headings : Caliciviridae Infections / diagnosis; Caliciviridae Infections / genetics; Epidemics / prevention & control; Humans; Norovirus / genetics; Norovirus / isolation & purification; Real-Time Polymerase Chain Reaction / methods; Recombinases / analysis; Recombinases / genetics; Reverse Transcriptase Polymerase Chain Reaction / methods
TL;DR: The reported RT-RPA method shows promise for sensitive point-of-care detection of epidemic human norovirus detection, and is the fastest human Norovirus amplification method to date. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2017 journal article

Efficacy of Neutral Electrolyzed Water for Inactivation of Human Norovirus

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 83(16).

By: E. Moorman n, N. Montazeri n & L. Jaykus n

author keywords: disinfection; environmental contamination; norovirus; public health; surrogate; virus inactivation
MeSH headings : Disinfection / instrumentation; Disinfection / methods; Electrolysis; Gastroenteritis / virology; Humans; Norovirus / drug effects; Norovirus / genetics; Norovirus / physiology; Viral Proteins / genetics; Viral Proteins / metabolism; Virus Inactivation / drug effects; Water / chemistry; Water / pharmacology
Source: Web Of Science
Added: August 6, 2018

2017 journal article

Evaluation of a Surface Sampling Method for Recovery of Human Noroviruses Prior to Detection Using Reverse Transcription Quantitative PCR

JOURNAL OF FOOD PROTECTION, 80(2), 231–236.

By: G. Tung-Thompson n, B. Escudero-Abarca n, J. Outlaw n, A. Ganee*, S. Cassard*, C. Mabilat*, L. Jaykus n

author keywords: Norovirus; Sampling; Surfaces; Wipes
MeSH headings : Caliciviridae Infections / virology; Feces / virology; Gastroenteritis / epidemiology; Humans; Norovirus / genetics; North Carolina; RNA, Viral; Reverse Transcriptase Polymerase Chain Reaction; Reverse Transcription
TL;DR: Wipes evaluated for their efficacy at recovering human norovirus from hard surfaces and foods may be a valuable tool for investigations of Norovirus outbreaks and studies of norov virus prevalence. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2017 journal article

Human norovirus binding to select bacteria representative of the human gut microbiota

PLOS ONE, 12(3).

By: E. Almand n, M. Moore n, J. Outlaw n & L. Jaykus n

MeSH headings : Bacillus / isolation & purification; Bacillus / metabolism; Citrobacter / isolation & purification; Citrobacter / metabolism; Culture Media / chemistry; Enterobacter cloacae / isolation & purification; Enterobacter cloacae / metabolism; Enterococcus faecium / isolation & purification; Enterococcus faecium / metabolism; Feces / microbiology; Fimbriae, Bacterial / chemistry; Fimbriae, Bacterial / metabolism; Fimbriae, Bacterial / ultrastructure; Gastrointestinal Microbiome / physiology; Hafnia alvei / isolation & purification; Hafnia alvei / metabolism; Humans; Klebsiella / isolation & purification; Klebsiella / metabolism; Microbial Interactions; Norovirus / metabolism; Norovirus / ultrastructure; Staphylococcus aureus / isolation & purification; Staphylococcus aureus / metabolism
TL;DR: The findings reported here further elucidate and inform the dynamics between human noroviruses and enteric bacteria with implications for norovirus pathogenesis. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2017 journal article

Keys to successful grant writing

Journal of Food Science, 82(7), 1511–1512.

By: L. Jaykus

Source: NC State University Libraries
Added: August 6, 2018

2017 journal article

Microbial Load of Fresh Produce and Paired Equipment Surfaces in Packing Facilities Near the US and Mexico Border

JOURNAL OF FOOD PROTECTION, 80(4), 582–589.

author keywords: Equipment; Farm; Microbial indicator; Packing; Produce
MeSH headings : Colony Count, Microbial; Enterococcus; Escherichia coli; Food Handling; Food Microbiology; Mexico
TL;DR: Equipment surface and produce microbial loads were correlated, but correlations varied from none to high depending on the equipment surface, and the importance of packing facility sanitation to protect produce quality and safety is confirmed. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2017 review

Norovirus Binding to Ligands Beyond Histo-Blood Group Antigens

[Review of ]. FRONTIERS IN MICROBIOLOGY, 8.

By: E. Almand n, M. Moore n & L. Jaykus n

author keywords: norovirus; histo-blood group antigens; virus-bacteria interaction; transkingdom; enteric virus
TL;DR: This review seeks to supplement the already well-covered HBGA-norovirus literature by covering non-HBGA human norovirus ligands and inhibitors to provide investigators with a more comprehensive view of norov virus ligands. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2017 journal article

Recombinase polymerase amplification: a promising point-of-care detection method for enteric viruses

FUTURE VIROLOGY, 12(8), 421–429.

By: M. Moore n & L. Jaykus n

author keywords: detection; enteric virus; in-field; point-of-care; recombinase polymerase amplification
TL;DR: This report reviews the use of recombinase polymerase amplification for virus detection, showing that the method has favorable fundamental properties supporting its promise for rapid point-of-care detection of enteric viruses. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2017 review

The effect of hand-hygiene interventions on infectious disease-associated absenteeism in elementary schools: A systematic literature review

[Review of ]. AMERICAN JOURNAL OF INFECTION CONTROL, 45(6), 682–689.

By: Z. Wang*, M. Lapinski*, E. Quilliam*, L. Jaykus n & A. Fraser*

author keywords: Acute gastroenteritis; Respiratory illness; Health education; Hand washing; Children
MeSH headings : Absenteeism; Child; Communicable Disease Control / methods; Communicable Diseases / epidemiology; Female; Hand Hygiene / methods; Humans; Male; Schools / statistics & numerical data
TL;DR: Evidence is indicated to show hand‐hygiene interventions had an effect on reducing acute gastrointestinal illness‐associated absenteeism but inadequate evidence is available to show anEffect on respiratory illness‐ associated absenteeism. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2017 journal article

Virucidal Activity of Fogged Chlorine Dioxide-and Hydrogen Peroxide-Based Disinfectants against Human Norovirus and Its Surrogate, Feline Calicivirus, on Hard-to-Reach Surfaces

FRONTIERS IN MICROBIOLOGY, 8.

author keywords: hydrogen peroxide; chlorine dioxide; fogged disinfectant; norovirus inactivation; surface disinfection; public health
TL;DR: Hydrogen peroxide delivered by fog showed promising virucidal activity against FCV by meeting the United States EPA 4-log10 reduction criteria for an anti-noroviral disinfectant; however, fogged chlorine dioxide-surfactant-based product did not achieve a 4- log10 inactivation. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2017 review

Virus-bacteria interactions: An emerging topic in human infection

[Review of ]. Viruses-Basel, 9(3).

By: E. Almand, M. Moore & L. Jaykus

Source: NC State University Libraries
Added: August 6, 2018

2016 review

A systematic review of human norovirus survival reveals a greater persistence of human norovirus RT-qPCR signals compared to those of cultivable surrogate viruses

[Review of ]. INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 216, 40–49.

By: A. Knight*, J. Haines*, A. Stals*, D. Li*, M. Uyttendaele*, A. Knight, L. Jaykus n

author keywords: Systematic review; Norovirus; RT-qPCR; Survival; Persistence; Heat; Chlorine
MeSH headings : Caliciviridae Infections / virology; Chlorine / pharmacology; Gastroenteritis / virology; Hot Temperature; Humans; Norovirus / isolation & purification; Norovirus / physiology; Real-Time Polymerase Chain Reaction; Temperature; Viral Load
TL;DR: Overall the data suggest that hNoV are frequently more resistant to typical food and environmental control measures compared with cultivable surrogate viruses, when basing data on comparative RT-qPCR results. (via Semantic Scholar)
UN Sustainable Development Goal Categories
10. Reduced Inequalities (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2016 journal article

Assessment of Risk Communication about Undercooked Hamburgers by Restaurant Servers

JOURNAL OF FOOD PROTECTION, 79(12), 2113–2118.

By: E. Thomas*, A. Binder n, A. Mclaughlin n, L. Jaykus n, D. Hanson n, D. Powell, B. Chapman n

Contributors: B. Chapman n, B. Chapman, D. Hanson n, D. Powell, L. Jaykus n, L. Jaykus, A. McLAUGHLIN n, E. Thomas*, A. Binder n

author keywords: Hamburger safety; Measuring doneness of hamburgers; Risk communication
MeSH headings : Animals; Cooking; Food Handling; Food Safety; Humans; Meat Products; Restaurants; United States
TL;DR: The majority of servers reported an unreliable method of doneness or other incorrect information related to burger doneness and safety, and the current risk communication language in the Model Food Code does not sufficiently fill these gaps. (via Semantic Scholar)
UN Sustainable Development Goal Categories
13. Climate Action (Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2016 journal article

Capture and concentration of viral and bacterial foodborne pathogens using apolipoprotein H

JOURNAL OF MICROBIOLOGICAL METHODS, 128, 88–95.

By: E. Almand n, R. Goulter n & L. Jaykus n

author keywords: ApoH; Detection; Norovirus; Bacteria; Concentration
MeSH headings : Colony Count, Microbial; DNA, Bacterial / isolation & purification; Escherichia coli O157 / isolation & purification; Food Contamination / analysis; Food Microbiology; Foodborne Diseases / diagnosis; Foodborne Diseases / microbiology; Foodborne Diseases / virology; Listeria monocytogenes / isolation & purification; Norwalk virus / isolation & purification; RNA, Viral / isolation & purification; Real-Time Polymerase Chain Reaction; Salmonella enteritidis / isolation & purification; Sequence Analysis, RNA; Staphylococcus aureus / isolation & purification; beta 2-Glycoprotein I / chemistry
TL;DR: ApoH has the potential to be a broadly reactive ligand for separating and concentrating representative foodborne pathogens, both bacteria and viruses. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2016 journal article

Efficacy of a disinfectant containing silver dihydrogen citrate against GI.6 and GII.4 human norovirus

JOURNAL OF APPLIED MICROBIOLOGY, 122(1), 78–86.

By: C. Manuel n, M. Moore n & L. Jaykus n

author keywords: antimicrobials; disinfection; environmental; food safety; norovirus; viruses
MeSH headings : Capsid Proteins / genetics; Citrates / pharmacology; Disinfectants / pharmacology; Humans; Norovirus / drug effects; Norovirus / genetics; Real-Time Polymerase Chain Reaction; Silver Compounds / pharmacology
TL;DR: This study tested the efficacy of an antimicrobial product containing a blend of silver ions and citric acid (silver dihydrogen citrate; SDC) against GI. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2016 journal article

Efficacy of two hygiene methods to reduce soil and microbial contamination on farmworker hands during harvest

FOOD CONTROL, 59, 787–792.

By: A. Aceituno*, N. Heredia*, A. Stern*, F. Bartz*, F. Venegas*, L. Solis-Soto*, J. Gentry-Shields n, L. Jaykus n, J. Leon*, S. Garcia*

author keywords: Foodbome disease; Hand hygiene; Alcohol-based hand sanitizer; Antimicrobial efficacy
TL;DR: The hand washing with soap group, compared to the control group, had significantly lower amounts of soil on hands and soil concentrations and the presence of soil and microbes on the hands of agricultural field workers. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2016 journal article

Human Norovirus Aptamer Exhibits High Degree of Target Conformation-Dependent Binding Similar to That of Receptors and Discriminates Particle Functionality

MSPHERE, 1(6).

By: M. Moore n, B. Bobay n, B. Mertens n & L. Jaykus n

author keywords: aptamer; GII.4; histo-blood group antigen; infectivity; noroviruses
TL;DR: A hitherto unexplored property of a different type of ligand, a nucleic acid aptamer, to mimic receptor binding behavior and assess capsid functionality for a selected strain of norovirus is reported, suggesting that other aptamers may show promise in the study of other fastidious microorganisms. (via Semantic Scholar)
UN Sustainable Development Goal Categories
10. Reduced Inequalities (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2016 journal article

Microbial Indicator Profiling of Fresh Produce and Environmental Samples from Farms and Packing Facilities in Northern Mexico

JOURNAL OF FOOD PROTECTION, 79(7), 1197–1209.

By: N. Heredia*, C. Caballero*, C. Cardenas*, K. Molina*, R. Garcia*, L. Solis*, V. Burrowes*, F. Bartz* ...

author keywords: Foodborne disease; Food safety; Microbiology
MeSH headings : Colony Count, Microbial; Escherichia coli O157; Farms; Food Microbiology; Fruit / microbiology; Mexico; Salmonella
TL;DR: The finding that microbial contamination on produce farms is influenced by produce type and production step can inform the design of effective approaches to mitigate microbial contamination. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2016 conference paper

Natural microbiota of raspberries (Rubus idaeus) and strawberries (Fragaria x ananassa): Microbial survey, bacterial isolation and identification, and biofilm characterization

Xi international rubus and ribes symposium, 1133, 521–526.

By: J. Baugher & L. Jaykus

Source: NC State University Libraries
Added: August 6, 2018

2016 article

Risk Assessment for Foodborne Viruses

VIRUSES IN FOODS, 2ND EDITION, pp. 471–503.

By: E. Bradshaw n & L. Jaykus n

TL;DR: This chapter provides an overview of the steps of risk analysis with a focus on risk assessment for foodborne viruses, particularly quantitative efforts that model and estimate the risks these viruses pose to human health. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2016 journal article

Somatic Coliphage Profiles of Produce and Environmental Samples from Farms in Northern M,xico

FOOD AND ENVIRONMENTAL VIROLOGY, 8(3), 221–226.

author keywords: Foodborne disease; Food safety; Microbiology; Viruses
MeSH headings : Adult; Capsicum / virology; Coliphages / classification; Coliphages / genetics; Coliphages / isolation & purification; Cucumis melo / virology; Farms; Female; Food Contamination / analysis; Fresh Water / virology; Fruit / virology; Hand / virology; Humans; Solanum lycopersicum / virology; Male; Mexico; Soil Microbiology
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2015 journal article

Aerosolization of a Human Norovirus Surrogate, Bacteriophage MS2, during Simulated Vomiting

PLOS ONE, 10(8), e0134277.

By: G. Tung-Thompson n, D. Libera n, K. Koch*, F. de los Reyes n & L. Jaykus n

Contributors: G. Tung-Thompson n, D. Libera n, K. Koch*, F. De Los Reyes n & L. Jaykus n

Ed(s): C. Bauch

MeSH headings : Aerosols / analysis; Caliciviridae Infections / complications; Caliciviridae Infections / transmission; Caliciviridae Infections / virology; Disease Outbreaks; Gastroenteritis / epidemiology; Gastroenteritis / virology; Humans; Levivirus / isolation & purification; Models, Biological; Norovirus / isolation & purification; Vomiting / complications; Vomiting / virology
TL;DR: This is the first study to document and measure aerosolization of a NoV surrogate in a similitude-based physical model, which has implications for better understanding the transmission dynamics of human NoV and for risk modeling purposes, both of which can help in designing effective infection control measures. (via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: August 6, 2018

2015 journal article

An observational study of frequency of provider hand contacts in child care facilities in North Carolina and South Carolina

AMERICAN JOURNAL OF INFECTION CONTROL, 43(2), 107–111.

By: A. Fraser*, K. Wohlgenant*, S. Cates*, . Chen*, L. Jaykus n, Y. Li n, B. Chapman n

Contributors: A. Fraser*, K. Wohlgenant*, S. Cates*, X. Chen*, L. Jaykus n, Y. Li n, B. Chapman n

author keywords: Child care providers; Porous surfaces; Nonporous surfaces; Hand contact; Observational study
MeSH headings : Child; Child Day Care Centers; Child, Preschool; Environmental Exposure; Fomites; Hand Disinfection; Humans; Infant; Infection Control / methods; North Carolina; Play and Playthings; Sanitation / methods; South Carolina
TL;DR: The frequency surfaces were touched by child care providers to identify surfaces that should be cleaned and sanitized to effectively treat porous surfaces was determined. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: August 6, 2018

2015 journal article

Associations between Weather and Microbial Load on Fresh Produce Prior to Harvest

JOURNAL OF FOOD PROTECTION, 78(4), 849–854.

By: M. Ward, R. Dhingra*, J. Remais*, H. Chang*, L. Johnston n, L. Jaykus n, J. Leon

MeSH headings : Bacterial Load; Crops, Agricultural / growth & development; Crops, Agricultural / microbiology; Enterococcus / isolation & purification; Escherichia coli / isolation & purification; Food Microbiology; Foodborne Diseases; Rain; Temperature; United States; Weather
TL;DR: Temperature and precipitation may have a positive but complex relationship with microbial indicators, as precipitation may create moist conditions conducive to bacterial growth, spread contamination onto the field, or wash contamination off of the plant. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2015 journal article

Comparison of process control viruses for use in extraction and detection of human norovirus from food matrices

FOOD RESEARCH INTERNATIONAL, 77, 320–325.

By: J. Gentry-Shields n & L. Jaykus n

author keywords: Norovirus; Process control virus; RT-qPCR; Processing efficiency
TL;DR: This study compared five PCVs to two HuNoV strains for recovery during the steps of elution, polyethylene glycol precipitation (PEG), and RNA extraction from select foods (lettuce and sliced deli ham), and found the virus that least mimicked the behavior of Hu noV during sample processing was MNV-1. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2015 journal article

Destruction of the Capsid and Genome of GII.4 Human Norovirus Occurs during Exposure to Metal Alloys Containing Copper

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 81(15), 4940–4946.

By: C. Manuel n, M. Moore n & L. Jaykus n

MeSH headings : Alloys / toxicity; Blotting, Western; Capsid / drug effects; Copper / toxicity; Feces / virology; Genome, Viral / drug effects; Humans; Microscopy, Electron, Transmission; Norovirus / drug effects; Norovirus / physiology; Real-Time Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; Virus Attachment / drug effects
TL;DR: It is suggested that copper surfaces destroy HuNoV and may be useful in preventing environmental transmission of the virus in at-risk settings. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2015 journal article

Generation and characterization of nucleic acid aptamers targeting the capsid P domain of a human norovirus GII.4 strain

JOURNAL OF BIOTECHNOLOGY, 209, 41–49.

By: M. Moore n, B. Escudero-Abarca n, S. Suh n & L. Jaykus n

author keywords: SELEX; Aptamer; Norovirus; Molecular detection; Viral diagnostics
MeSH headings : Aptamers, Nucleotide / chemistry; Aptamers, Nucleotide / metabolism; Capsid Proteins / genetics; Capsid Proteins / metabolism; Feces / virology; Humans; Norovirus / classification; Norovirus / genetics; Norovirus / isolation & purification; SELEX Aptamer Technique / methods; Sensitivity and Specificity
TL;DR: This is the first demonstration of human NoV P domain protein as a functional target for the selection of nucleic acid aptamers that specifically bind and broadly recognize diversehuman NoV strains. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2015 article

Human Norovirus as a Foodborne Pathogen: Challenges and Developments

ANNUAL REVIEW OF FOOD SCIENCE AND TECHNOLOGY, VOL 6, Vol. 6, pp. 411–433.

By: M. Moore n, R. Goulter n & L. Jaykus n

author keywords: epidemiology; detection; evolution; cell culture; infectivity
MeSH headings : Animals; Caliciviridae Infections / epidemiology; Caliciviridae Infections / virology; Cell Line; Food / virology; Gastroenteritis / epidemiology; Gastroenteritis / virology; Humans; Norovirus / classification; Norovirus / pathogenicity; Phylogeny
TL;DR: A brief overview of NoVs and their genetic structure is provided and the challenges and recent developments related to human NoVs regarding viral evolution, transmission, epidemiology, outbreak identification, cultivation, animal and human models, and detection are presented. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2015 review

Microbial Hazards in Irrigation Water: Standards, Norms, and Testing to Manage Use of Water in Fresh Produce Primary Production

[Review of ]. COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY, 14(4), 336–356.

By: M. Uyttendaele*, L. Jaykus n, P. Amoah*, A. Chiodini*, D. Cunliffe, L. Jacxsens*, K. Holvoet*, L. Korsten* ...

author keywords: water; irrigation; outbreaks; fresh produce; good practices; testing
Source: Web Of Science
Added: August 6, 2018

2015 journal article

Validation of a novel rinse and filtration method for efficient processing of fresh produce samples for microbiological indicator enumeration

Journal of Food Protection, 78(3), 525–530.

MeSH headings : Bacteria / isolation & purification; Capsicum / microbiology; Colony Count, Microbial; Consumer Product Safety; Cucumis melo / microbiology; Cucurbitaceae / microbiology; Decontamination / methods; Enterococcus faecalis / isolation & purification; Escherichia coli / isolation & purification; Filtration; Food Handling / methods; Food Microbiology; Fruit / microbiology; Salmonella typhimurium / isolation & purification; Solutions
TL;DR: The rinse and filtration method performed equivalent to, and sometimes better than, either of the compared methods, and may have logistical advantages when processing large numbers of samples, improving sampling efficiency and facilitating microbial detection. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: NC State University Libraries
Added: August 6, 2018

2015 review

Zero Risk Does Not Exist: Lessons Learned from Microbial Risk Assessment Related to Use of Water and Safety of Fresh Produce

[Review of ]. COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY, 14(4), 387–410.

By: A. De Keuckelaere*, L. Jacxsens*, P. Amoah*, G. Medema*, P. McClure*, L. Jaykus n, M. Uyttendaele*

author keywords: fruits and vegetables; health risk; mitigation strategies; quantitative microbial risk assessment; water
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2014 journal article

Development and evaluation of aptamer magnetic capture assay in conjunction with real-time PCR for detection of Campylobacter jejuni

LWT-FOOD SCIENCE AND TECHNOLOGY, 56(2), 256–260.

By: S. Suh n, H. Dwivedi n & L. Jaykus n

author keywords: Aptamer; Aptamer magnetic capture; Campylobacter jejuni; Real-time PCR; Pathogen detection
TL;DR: A prototype method for the concentration and detection of Campylobacter jejuni was developed using a previously reported biotinylated DNA aptamer in conjunction with qPCR, and nucleic acid aptamers are promising alternatives to antibodies for magnetic bead-based capture followed byqPCR detection. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2014 article

Editorial overview: Environmental virology

Jaykus, L.-A., & Meschke, J. S. (2014, February). CURRENT OPINION IN VIROLOGY, Vol. 4, pp. VII-IX.

By: L. Jaykus n & J. Meschke*

MeSH headings : Disease Transmission, Infectious / prevention & control; Disinfection / methods; Environmental Microbiology; Humans; Virus Diseases / transmission; Viruses / isolation & purification
Source: Web Of Science
Added: August 6, 2018

2014 journal article

Hygienic conditions in child-care facilities in North Carolina and South Carolina: An integrated microbial and observational study

AMERICAN JOURNAL OF INFECTION CONTROL, 42(7), 781–786.

By: Y. Li n, L. Jaykus n, S. Cates n, K. Wohlgenant*, . Chen* & A. Fraser*

author keywords: Microbial indicators; Sanitation; Family daycare home; Child care centers
MeSH headings : Bacteria, Aerobic / isolation & purification; Bacterial Load; Child Day Care Centers; Child, Preschool; Enterobacteriaceae / isolation & purification; Environmental Microbiology; Female; Humans; Infant; Infection Control / methods; Male; North Carolina; Sanitation / methods; South Carolina
TL;DR: This unique study, which combined observational and microbiological data, provided revealing information about the relationship between hygiene indicators and sanitary practices in child-care facilities in the southeastern United States. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2014 journal article

Microbiological analysis of environmental samples collected from child care facilities in North and South Carolina

AMERICAN JOURNAL OF INFECTION CONTROL, 42(10), 1049–1055.

By: Y. Li n, A. Fraser*, . Chen*, S. Cates*, K. Wohlgenant* & L. Jaykus n

author keywords: Childcare facilities; Day care; Environmental sampling; Microbiological indicators; Enteric pathogens
MeSH headings : Bacteria / isolation & purification; Caregivers; Child Day Care Centers; Child, Preschool; Environmental Microbiology; Female; Hand / microbiology; Hand / virology; Humans; Infant; Infection Control / methods; Male; Norovirus / isolation & purification; North Carolina; South Carolina
TL;DR: The relative absence of pathogens and biotype I E coli in environmental samples suggests the child care facilities sampled in this study managed fecal contamination well. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2014 journal article

Molecular methods used to estimate thermal inactivation of a prototype human norovirus: More heat resistant than previously believed?

FOOD MICROBIOLOGY, 41, 91–95.

By: B. Escudero-Abarca n, H. Rawsthorne n, R. Goulter n, S. Suh n & L. Jaykus n

author keywords: Human norovirus; Thermal inactivation; Heat resistance
MeSH headings : Azides / chemistry; Caliciviridae Infections / virology; Hot Temperature; Humans; Norovirus / chemistry; Norovirus / genetics; Norovirus / isolation & purification; Norovirus / physiology; Polymerase Chain Reaction; Propidium / analogs & derivatives; Propidium / chemistry; RNA, Viral / chemistry; RNA, Viral / genetics; Virus Inactivation
TL;DR: Electron microscopy data applied to heat-treated virus-like particles supported this relatively high degree of thermal resistance and suggest that SMV is more heat resistant than common cultivable HuNoV surrogates. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2014 journal article

Persistence of Human Norovirus RT-qPCR Signals in Simulated Gastric Fluid

FOOD AND ENVIRONMENTAL VIROLOGY, 7(1), 32–40.

By: G. Tung-Thompson n, J. Gentry-Shields n, A. Fraser* & L. Jaykus n

author keywords: Norovirus; Persistence; Simulated gastric fluid; Vomitus
MeSH headings : Acids / pharmacology; Gastric Acid / chemistry; Humans; Hydrogen-Ion Concentration; Models, Biological; Norovirus / drug effects; Norovirus / genetics; Norovirus / isolation & purification; Real-Time Polymerase Chain Reaction
TL;DR: It is suggested that HuNoV suspended in vomitus-like material can persist for long periods, a likely contributor to foodborne transmission. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2014 journal article

Sanitation in classroom and food preparation areas in child-care facilities in North Carolina and South Carolina

Journal of Environmental Health, 77(4), 20–27.

By: K. Wohlgenant, S. Cates, A. Fraser, B. Chapman, L. Jaykus & X. Chen

Source: NC State University Libraries
Added: August 6, 2018

2014 journal article

Selection and characterization of DNA aptamers specific for Listeria species

ANALYTICAL BIOCHEMISTRY, 459, 39–45.

By: S. Suh n, H. Dwivedi n, S. Choi n & L. Jaykus n

author keywords: Listeria monocytogenes; Listeria spp.; Aptamer; SELEX; Pathogen detection
MeSH headings : Aptamers, Nucleotide / chemistry; Aptamers, Nucleotide / genetics; Aptamers, Nucleotide / metabolism; Base Sequence; Binding Sites; Cell Survival; Listeria monocytogenes / cytology; Listeria monocytogenes / metabolism; SELEX Aptamer Technique / methods; Species Specificity; Substrate Specificity
TL;DR: This is the first study in which SELEX targeted bacterial cells at different growth phases and found that aptamers that bind to different cell surface moieties could increase the analytical sensitivity of future capture and detection assays. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2014 journal article

Selection, Characterization and Application of Nucleic Acid Aptamers for the Capture and Detection of Human Norovirus Strains

PLOS ONE, 9(9).

By: B. Escudero-Abarca n, S. Suh n, M. Moore n, H. Dwivedi n & L. Jaykus n

MeSH headings : Aptamers, Nucleotide / chemistry; Aptamers, Nucleotide / metabolism; Bacteriological Techniques / methods; DNA, Viral / chemistry; DNA, Viral / metabolism; Feces / virology; Food Microbiology; Humans; Lettuce / virology; Molecular Structure; Norovirus / classification; Norovirus / genetics; Norovirus / isolation & purification; Sensitivity and Specificity
TL;DR: These ssDNA aptamer candidates show promise as broadly reactive reagents for use in HuNoV capture and detection assays in various sample types. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2014 journal article

Tracing Temperature Patterns of Cut Leafy Greens during Service in North Carolina School Food Service

JOURNAL OF FOOD PROTECTION, 77(9), 1495–1500.

By: E. Thomas*, B. Chapman n, L. Jaykus* & T. Phister*

Contributors: E. Thomas*, B. Chapman n, L. Jaykus* & T. Phister*

MeSH headings : Food Contamination / analysis; Food Handling / instrumentation; Food Handling / methods; Food Handling / standards; Food Services / standards; Humans; Lettuce / microbiology; North Carolina; Plant Leaves / microbiology; Schools / statistics & numerical data; Spinacia oleracea / microbiology; Temperature; Vegetables / microbiology
TL;DR: The results demonstrate the importance of developing a protocol for continuous temperature monitoring of leafy greens served in school lunch programs and demonstrate the two policies that North Carolina has in place to control the temperature of these products. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: August 6, 2018

2014 journal article

Use of Bacteroidales Microbial Source Tracking To Monitor Fecal Contamination in Fresh Produce Production

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 80(2), 612–617.

By: K. Ravaliya n, J. Gentry-Shields n, S. Garcia*, N. Heredia*, A. Aceituno*, F. Bartz*, J. Leon*, L. Jaykus n

MeSH headings : Agricultural Irrigation / methods; Agriculture; Bacteroidetes / genetics; Capsicum / microbiology; Cucumis melo / microbiology; Escherichia coli / genetics; Feces / microbiology; Food Contamination / analysis; Food Microbiology / methods; Genetic Markers; Humans; Mexico; RNA, Ribosomal, 16S; Vegetables / microbiology
TL;DR: Evidence is provided that Bacteroidales markers may serve as alternative indicators for fecal contamination in fresh produce production, allowing for determination of both general contamination and that derived from the human host. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2013 journal article

Efficacy of Commonly Used Disinfectants for Inactivation of Human Noroviruses and Their Surrogates

JOURNAL OF FOOD PROTECTION, 76(7), 1210–1217.

By: G. Tung n, D. Macinga, J. Arbogast & L. Jaykus*

MeSH headings : Caliciviridae Infections / virology; Colony Count, Microbial; Disinfectants / pharmacology; Disinfection / methods; Dose-Response Relationship, Drug; Drug Resistance, Viral; Ethanol / pharmacology; Food Safety; Gastroenteritis / virology; Humans; Norovirus / drug effects; Norovirus / growth & development; Quaternary Ammonium Compounds / pharmacology; Sodium Hypochlorite / pharmacology; Virus Inactivation / drug effects
TL;DR: This study clearly demonstrates the relative ineffectiveness of common active disinfectant ingredients against Hu noV and highlights the fact that the cultivable surrogates do not always mimic HuNoV strains. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2013 journal article

Laboratory Evidence of Norwalk Virus Contamination on the Hands of Infected Individuals

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 79(24), 7875–7881.

By: P. Liu*, B. Escudero n, L. Jaykus n, J. Montes, R. Goulter n, M. Lichtenstein*, M. Fernandez*, J. Lee* ...

MeSH headings : Caliciviridae Infections / virology; Gastroenteritis / virology; Hand / virology; Humans; Norwalk virus / isolation & purification; RNA, Viral / genetics; RNA, Viral / isolation & purification; Real-Time Polymerase Chain Reaction; Viral Load
TL;DR: Findings provide definitive evidence of NV contamination on the hands of infected subjects observed under controlled clinical research conditions, and support the need for better hand hygiene strategies to prevent NoV transmission. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2013 journal article

Nucleic acid aptamers for capture and detection of Listeria spp

JOURNAL OF BIOTECHNOLOGY, 167(4), 454–461.

By: S. Suh n & L. Jaykus*

author keywords: Listeria monocytogenes; Listeria spp; DNA aptamer; Aptamer magnetic capture; Immunomagnetic separation; Real-time PCR
MeSH headings : Aptamers, Nucleotide / chemical synthesis; Aptamers, Nucleotide / economics; Bacteriological Techniques / methods; Base Sequence; Biotinylation; Flow Cytometry; Listeria monocytogenes / isolation & purification; Molecular Sequence Data; Real-Time Polymerase Chain Reaction
TL;DR: Biotinylated ssDNA aptamers are promising ligands for food-borne pathogen concentration prior to detection using molecular methods and showed similar binding affinity for other members of the Listeria genus and lowbinding affinity for non-Listeria species. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2013 journal article

Selection of DNA aptamers for capture and detection of Salmonella Typhimurium using a whole-cell SELEX approach in conjunction with cell sorting

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 97(8), 3677–3686.

By: H. Dwivedi n, R. Smiley n & L. Jaykus n

author keywords: Salmonella; DNA aptamers; Real-time PCR; Whole-cell SELEX
MeSH headings : Aptamers, Nucleotide; Bacteriological Techniques / methods; Cross Reactions; Flow Cytometry / methods; Food Microbiology / methods; Salmonella typhimurium / isolation & purification; Sensitivity and Specificity
TL;DR: This study provides proof-of-concept that biotinylated aptamers selected by whole-cell SELEX can be used in a qPCR-based capture-detection platform for Salmonella Typhimurium. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2012 journal article

Persistence and Transferability of Noroviruses on and between Common Surfaces and Foods

JOURNAL OF FOOD PROTECTION, 75(5), 927–935.

By: B. Escudero n, H. Rawsthorne n, C. Gensel n & L. Jaykus n

MeSH headings : Colony Count, Microbial; Consumer Product Safety; Equipment Contamination; Food Contamination / analysis; Food Handling / methods; Food Microbiology; Humans; Hygiene; Norovirus / growth & development; Reverse Transcriptase Polymerase Chain Reaction; Risk Assessment; Time Factors; Viral Plaque Assay
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2012 journal article

Persistence of Norwalk Virus, Male-Specific Coliphage, and Escherichia coli on Stainless Steel Coupons and in Phosphate-Buffered Saline

JOURNAL OF FOOD PROTECTION, 75(12), 2151–2157.

By: P. Liu*, L. Jaykus n, E. Wong* & C. Moe*

MeSH headings : Coliphages / growth & development; Consumer Product Safety; Equipment Contamination; Escherichia coli / growth & development; Food Contamination / analysis; Food Microbiology; Humans; Hydrogen-Ion Concentration; Models, Biological; Norwalk virus / growth & development; Stainless Steel; Temperature; Time Factors
TL;DR: Findings indicate that NoVs are highly persistent on environmental surfaces and in PBS solution at different temperatures, and E. coli does not appear to be an appropriate surrogate for NoVs, MS2 could be more relevant for modeling the environmental persistence of NoVs under wet conditions, but not under dry conditions. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2011 journal article

Pathogen-produce pair attribution risk ranking tool to prioritize fresh produce commodity and pathogen combinations for further evaluation (P(3)ARRT)

FOOD CONTROL, 22(12), 1865–1872.

By: M. Anderson*, L. Jaykus n, S. Beaulieu* & S. Dennis*

author keywords: Fresh produce; Pathogens; Risk ranking
TL;DR: A semi-quantitative risk ranking tool to rank the relative public health impact of pathogen-produce commodity combinations, based on explicit data-driven criteria, and enterohemorrhagic E. coli in leafy greens consistently ranked first, followed by Salmonella spp. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2011 journal article

Porcine parvovirus removal using trimer and biased hexamer peptides

Biotechnology Journal, 7(4), 558–565.

By: C. Heldt n, P. Gurgel n, L. Jaykus n & R. Carbonell n

author keywords: Affinity adsorption; Biosafety; Bioseparation; Ligand; Virus removal
MeSH headings : Adsorption; Chromatography, Affinity; Humans; Parvovirus, Porcine / chemistry; Parvovirus, Porcine / isolation & purification; Peptides / chemical synthesis; Peptides / chemistry; Plasma / chemistry; Plasma / virology
TL;DR: This study demonstrates that trimeric and hexameric peptides may have different, matrix‐specific roles to play in virus removal applications, and in general, the hexamer ligand may perform better for binding of specific viruses, whereas the trimer ligand might have more broadly reactive virus‐binding properties. (via Semantic Scholar)
Sources: Crossref, Web Of Science
Added: August 6, 2018

2010 review

Climate change and food safety: A review

[Review of ]. FOOD RESEARCH INTERNATIONAL, 43(7), 1745–1765.

By: M. Tirado*, R. Clarke*, L. Jaykus n, A. McQuatters-Gollop* & J. Franke

author keywords: Climate change; Food safety; Food control; Foodborne diseases; Microbiological contamination; Zoonosis-animal health; Plant Health; Biotoxins-mycotoxins; HABs-marine toxins; Chemical contamination; Surveillance-monitoring; Emergency response; Risk assessment; Predictive modeling
Source: Web Of Science
Added: August 6, 2018

2010 review

Detection of pathogens in foods: the current state-of-the-art and future directions

[Review of ]. CRITICAL REVIEWS IN MICROBIOLOGY, 37(1), 40–63.

By: H. Dwivedi n & L. Jaykus*

MeSH headings : Bacteria / genetics; Bacteria / isolation & purification; Bacteriological Techniques / economics; DNA, Bacterial / genetics; Enzyme-Linked Immunosorbent Assay; Food Microbiology / economics; Food Microbiology / methods; Nucleic Acid Amplification Techniques; Polymerase Chain Reaction
TL;DR: This article reviews recent advancements in food borne pathogen detection and discusses future methods with a focus on pre-analytical sample processing, culture independent methods, and biosensors. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2010 journal article

Development of molecular-based methods for determination of high histamine producing bacteria in fish

International Journal of Food Microbiology, 139(3), 161–167.

By: K. Björnsdóttir-Butler n, G. Bolton n, L. Jaykus n, P. McClellan-Green n & D. Green n

author keywords: Histamine; Bacteria; DNA probes; Colony lift hybridization; Fish
MeSH headings : Agar / analysis; Animals; Colony Count, Microbial; Culture Media; DNA Probes; DNA, Bacterial / analysis; Digoxigenin / chemistry; Food Contamination; Food Microbiology; Foodborne Diseases / microbiology; Genes, Bacterial; Gram-Negative Bacteria / genetics; Gram-Negative Bacteria / isolation & purification; Histamine / analysis; Histidine Decarboxylase / genetics; Polymerase Chain Reaction; Seafood / microbiology
TL;DR: The use of digoxigenin (DIG) labeled DNA probes with colony lift hybridization shows promise for accurate and specific enumeration of histamine producing bacteria in scombroid fish. (via Semantic Scholar)
Sources: Crossref, Web Of Science
Added: August 6, 2018

2010 journal article

Effectiveness of Liquid Soap and Hand Sanitizer against Norwalk Virus on Contaminated Hands

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 76(2), 394–399.

By: P. Liu*, Y. Yuen*, H. Hsiao*, L. Jaykus n & C. Moe*

MeSH headings : Adult; Disinfectants / pharmacology; Ethanol / pharmacology; Hand / microbiology; Humans; Norwalk virus / drug effects; Norwalk virus / genetics; Norwalk virus / isolation & purification; Reproducibility of Results; Reverse Transcriptase Polymerase Chain Reaction; Soaps / pharmacology; Sodium Hypochlorite / pharmacology
TL;DR: Despite the promise of alcohol-based sanitizers for the control of pathogen transmission, they may be relatively ineffective against the HuNoV, reinforcing the need to develop and evaluate new products against this important group of viruses. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2010 journal article

Quantification of Norwalk Virus Inocula: Comparison of Endpoint Titration and Real-Time Reverse Transcription-PCR Methods

JOURNAL OF MEDICAL VIROLOGY, 82(9), 1612–1616.

By: P. Liu*, H. Hsiao*, L. Jaykus n & C. Moe*

author keywords: Norwalk virus; RT-qPCR; inoculum; endpoint titration
MeSH headings : Feces / virology; Gastroenteritis / virology; Humans; Limit of Detection; Norwalk virus / genetics; Norwalk virus / isolation & purification; RNA, Viral / analysis; Reverse Transcriptase Polymerase Chain Reaction; Sensitivity and Specificity
TL;DR: RT‐qPCR was 1.1–1.6 log10 more sensitive than endpoint titration RT‐PCR when the same RNA release method, PCR primers and thermocycle program were used, which has important implications for many experimental interpretations, not the least of which is estimating the median infectious dose in human challenge studies. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2010 journal article

Selection and characterization of DNA aptamers with binding selectivity to Campylobacter jejuni using whole-cell SELEX

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 87(6), 2323–2334.

By: H. Dwivedi n, R. Smiley n & L. Jaykus n

author keywords: Food-borne pathogen; Molecular-based assay; Rapid microbial method; Aptamers
MeSH headings : Animals; Aptamers, Nucleotide / chemistry; Aptamers, Nucleotide / genetics; Campylobacter jejuni / chemistry; Campylobacter jejuni / genetics; Campylobacter jejuni / isolation & purification; Kinetics; Nucleic Acid Conformation; Poultry / microbiology; SELEX Aptamer Technique / methods; Species Specificity
TL;DR: Whole-cell SELEX is a promising technique to design aptamer-based molecular probes for microbial pathogens without tedious isolation and purification of complex markers or targets. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2009 journal article

Correlation of Mannitol Fermentation with Virulence-Associated Genotypic Characteristics inVibrio vulnificusIsolates from Oysters and Water Samples in the Gulf of Mexico

Foodborne Pathogens and Disease, 7(1), 97–101.

By: S. Drake n, B. Whitney n, J. Levine n, A. DePaola* & L. Jaykus n

MeSH headings : Animals; Atlantic Ocean; Fermentation; Food Microbiology; Foodborne Diseases / prevention & control; Genotype; Louisiana; Mannitol / metabolism; Ostreidae / microbiology; Phenotype; RNA, Ribosomal, 16S / genetics; RNA, Ribosomal, 16S / metabolism; Seafood / microbiology; Seasons; Seawater / microbiology; Species Specificity; Statistics as Topic; Vibrio vulnificus / growth & development; Vibrio vulnificus / isolation & purification; Vibrio vulnificus / metabolism; Vibrio vulnificus / pathogenicity; Virulence / genetics
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Sources: Web Of Science, Crossref, NC State University Libraries
Added: August 6, 2018

2009 journal article

Detection of Gram-Negative Histamine-Producing Bacteria in Fish: A Comparative Study

JOURNAL OF FOOD PROTECTION, 72(9), 1987–1991.

By: K. Bjornsdottir n, G. Bolton n, P. McClellan-Green n, L. Jaykus n & D. Green n

MeSH headings : Agar; Animals; Chromatography, High Pressure Liquid; Colony Count, Microbial / methods; Consumer Product Safety; Fishes / microbiology; Food Contamination / analysis; Foodborne Diseases / microbiology; Gram-Negative Bacteria / isolation & purification; Gram-Negative Bacteria / metabolism; Histamine / biosynthesis; Histamine / toxicity; Humans; Polymerase Chain Reaction; Predictive Value of Tests; Seafood / microbiology; Sensitivity and Specificity
TL;DR: There was a high degree of concordance between results of the potentiometric and PCR methods, but neither of these methods detected low-histamine bacteria. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2009 journal article

Development of a Fluorescent In Situ Method for Visualization of Enteric Viruses

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 75(24), 7822–7827.

By: H. Rawsthorne n, T. Phister n & L. Jaykus n

MeSH headings : Animals; Biotin; Cell Line; Hepatitis A virus / isolation & purification; Hepatitis A virus / ultrastructure; Humans; Indicators and Reagents; Mice; Microscopy, Confocal / methods; Microscopy, Fluorescence / methods; Norovirus / isolation & purification; Norovirus / ultrastructure; Onions / virology; Quantum Dots; Streptavidin
TL;DR: The biotinylation process did not impact virus infectivity, nor did it interfere with the interactions between the virus and host cells or model produce items, and the method provides a powerful tool for the labeling and detection of enteric viruses (and their surrogates) which can be used to track virus behavior in situ. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2009 journal article

Development of a Risk-Ranking Framework to Evaluate Potential High-Threat Microorganisms, Toxins, and Chemicals in Food

JOURNAL OF FOOD SCIENCE, 74(2), R39–R45.

By: R. Newsome n, N. Tran n, G. Paoli n, L. Jaykus n, B. Tompkin n, M. Miliotis n, T. Ruthman n, E. Hartnett n ...

author keywords: food safety; risk; risk ranking
MeSH headings : Computer Simulation; Eggs / microbiology; Food / standards; Food Analysis; Food Handling / standards; Foodborne Diseases / prevention & control; Humans; Listeria monocytogenes / isolation & purification; Monte Carlo Method; Risk Assessment / methods; Salmonella / isolation & purification; United States; United States Food and Drug Administration
TL;DR: The Institute of Food Technologists developed a risk-ranking framework prototype to enable comparison of microbiological and chemical hazards in foods and to assist policy makers, risk managers, risk analysts, and others in determining the relative public health impact of specific hazard-food combinations. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2009 book

Food-borne microbes: Shaping the host ecosystem

Lee-Ann Jaykus

UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: NC State University Libraries
Added: August 6, 2018

2009 journal article

Impact of Storage Temperature and Product pH on the Survival of Listeria monocytogenes in Vacuum-Packaged Souse

JOURNAL OF FOOD PROTECTION, 72(3), 637–643.

By: M. Kim n, W. Bang n, M. Drake n, D. Hanson n & L. Jaykus n

MeSH headings : Animals; Colony Count, Microbial; Consumer Product Safety; Food Contamination / analysis; Food Contamination / prevention & control; Food Microbiology; Food Packaging / methods; Food Preservation / methods; Humans; Hydrogen-Ion Concentration; Listeria monocytogenes / growth & development; Meat Products / microbiology; Swine; Temperature; Time Factors; Vacuum
TL;DR: It is indicated that conventionally produced souse does not support the growth of L. monocytogenes and that inactivation of the organism is more likely in products formulated at a lower pH (< or = 4.3) without affecting consumer acceptance. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2009 journal article

Influence of Peptide Ligand Surface Density and Ethylene Oxide Spacer Arm on the Capture of Porcine Parvovirus

BIOTECHNOLOGY PROGRESS, 25(5), 1411–1418.

By: C. Heldt n, P. Gurgel n, L. Jaykus n & R. Carbonell n

author keywords: viral clearance; affinity resins; parvovirus; affinity ligands; combinatorial libraries
MeSH headings : Adsorption; Cells, Cultured; Chromatography, Affinity / methods; Ethylene Oxide / chemistry; Humans; Ligands; Particle Size; Parvovirus, Porcine / isolation & purification; Peptides / chemistry; Plasma / chemistry; Surface Properties
TL;DR: Results indicate that low peptide densities enhance binding selectivity, facilitating specific peptide‐virus binding even in the presence of plasma proteins which can theoretically bind nonspecifically. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2009 journal article

PCR-Based Method Using Propidium Monoazide To Distinguish Viable from Nonviable Bacillus subtilis Spores

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 75(9), 2936–2939.

By: H. Rawsthorne n, C. Dock n & L. Jaykus n

MeSH headings : Azides / metabolism; Azides / pharmacology; Bacillus subtilis / genetics; Bacillus subtilis / metabolism; Bacillus subtilis / physiology; DNA / metabolism; Fluorescent Dyes / metabolism; Fluorescent Dyes / pharmacology; Microbial Viability; Polymerase Chain Reaction / methods; Propidium / analogs & derivatives; Propidium / metabolism; Propidium / pharmacology; Spores / genetics; Spores / metabolism; Spores / physiology
TL;DR: A molecular-based method which is able to discriminate between viable and inactivated Bacillus subtilis spores by utilizing the DNA-intercalating dye propidium monoazide is described. (via Semantic Scholar)
UN Sustainable Development Goal Categories
10. Reduced Inequalities (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2009 journal article

Persistence of Human Noroviruses on Food Preparation Surfaces and Human Hands

FOOD AND ENVIRONMENTAL VIROLOGY, 1(3-4), 141–147.

By: P. Liu*, Y. Chien*, E. Papafragkou n, H. Hsiao*, L. Jaykus n & C. Moe*

author keywords: Norovirus; Real-time RT-PCR; Persistence; Environment
TL;DR: The results support the epidemiological evidence that food preparation surfaces and human hands can act as vehicles for human NoV transmission long after the initial contamination event has occurred. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2009 journal article

Quantitative exposure model for the transmission of norovirus in retail food preparation

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 133(1-2), 38–47.

By: A. Mokhtari* & L. Jaykus n

author keywords: Probabilistic risk assessment; Foodborne disease; Cross-contamination; Personal hygiene; Food handier; Sensitivity analysis
MeSH headings : Caliciviridae Infections / prevention & control; Caliciviridae Infections / transmission; Disease Reservoirs / virology; Feces / virology; Food Contamination / analysis; Food Contamination / prevention & control; Food Handling / standards; Foodborne Diseases / prevention & control; Foodborne Diseases / virology; Gastroenteritis / prevention & control; Gastroenteritis / virology; Hand Disinfection / standards; Humans; Hygiene / standards; Models, Theoretical; Norovirus
TL;DR: A probabilistic exposure assessment which models the dynamics of the transmission of HuNoV in the retail food preparation environment and suggests that gloving and hand-washing compliance are most effective in controlling contamination of food products when practiced simultaneously. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2009 review

Sample Preparation: The Forgotten Beginning

[Review of ]. JOURNAL OF FOOD PROTECTION, 72(8), 1774–1789.

By: B. Brehm-Stecher*, C. Young*, L. Jaykus n & M. Tortorello*

MeSH headings : Bacteriological Techniques; Food Contamination / prevention & control; Food Handling / methods; Food Microbiology; Food Technology; Humans
TL;DR: The purpose of this review is to present the current state of sample preparation, to identify knowledge gaps and opportunities for improvement, and to recognize the need to support greater research and development efforts on preparative methods in food microbiology. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2009 journal article

Selection, characterization, and application of DNA aptamers for the capture and detection of Salmonella enterica serovars

MOLECULAR AND CELLULAR PROBES, 23(1), 20–28.

By: R. Joshi*, H. Janagama*, H. Dwivedi n, T. Kumar*, L. Jaykus n, J. Schefers*, S. Sreevatsan*

author keywords: Salmonella; Pre-analytical processing; Aptamers; Food borne pathogens; Detection
MeSH headings : Animals; Aptamers, Nucleotide / genetics; Aptamers, Nucleotide / isolation & purification; Base Sequence; Blotting, Western; Chickens; DNA Footprinting; Deoxyribonucleases / metabolism; Electrophoretic Mobility Shift Assay; Mass Spectrometry; Membrane Proteins / chemistry; Molecular Sequence Data; SELEX Aptamer Technique; Salmonella enterica / classification; Salmonella enterica / genetics; Salmonella enterica / isolation & purification
TL;DR: The applicability of Salmonella specific aptamers for pre-analytical sample processing as applied to food and environmental sample matrices is demonstrated. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2008 journal article

Determination of molecular phylogenetics of Vibrio parahaemolyticus strains by multilocus sequence typing

JOURNAL OF BACTERIOLOGY, 190(8), 2831–2840.

MeSH headings : Bacterial Typing Techniques / methods; Cluster Analysis; DNA Fingerprinting; DNA, Bacterial / chemistry; DNA, Bacterial / genetics; Environmental Microbiology; Genes, Bacterial; Genetic Variation; Genotype; Humans; Molecular Epidemiology; Molecular Sequence Data; Phylogeny; Sequence Analysis, DNA; Vibrio Infections / microbiology; Vibrio parahaemolyticus / classification; Vibrio parahaemolyticus / genetics; Vibrio parahaemolyticus / isolation & purification
TL;DR: The data reported in this study indicate that V. parahaemolyticus is genetically diverse with a semiclonal population structure and an epidemic structure similar to that of Vibrio cholerae. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2008 journal article

Effectiveness of icing as a postharvest treatment for control of Vibrio vulnificus and Vibrio parahaemolyticus in the eastern oyster (Crassostrea virginica)

JOURNAL OF FOOD PROTECTION, 71(7), 1475–1480.

By: K. Melody*, R. Senevirathne*, M. Janes*, L. Jaykus n & J. Supan*

MeSH headings : Analysis of Variance; Animals; Colony Count, Microbial; Consumer Product Safety; Crassostrea / microbiology; Factor Analysis, Statistical; Food Contamination / analysis; Food Contamination / prevention & control; Food Handling / methods; Food Handling / standards; Food Preservation / methods; Humans; Ice; Seasons; Shellfish / microbiology; Time Factors; Vibrio parahaemolyticus / growth & development; Vibrio vulnificus / growth & development
TL;DR: On-board and dockside icing did not predictably reduce the levels of V. vulnificus or V. parahaemolyticus in oysters, and icing negatively impacted oyster survival during subsequent cold storage. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2008 article

Identification of trimeric peptides that bind porcine parvovirus from mixtures containing human blood plasma

BIOTECHNOLOGY PROGRESS, Vol. 24, pp. 554–560.

By: C. Heldt n, P. Gurgel*, L. Jaykus* & R. Carbonell*

MeSH headings : Animals; Complex Mixtures / isolation & purification; Complex Mixtures / metabolism; Humans; Oligopeptides / metabolism; Parvovirus, Porcine / isolation & purification; Parvovirus, Porcine / metabolism; Plasma / metabolism; Plasma / virology
TL;DR: The results of this work indicate that small peptide ligand resins have the potential to be used in virus removal processes where removal of contaminating virus is necessary to ensure product safety. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2008 journal article

Improved inactivation of nonenveloped enteric viruses and their surrogates by a novel alcohol-based hand sanitizer

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 74(16), 5047–5052.

MeSH headings : Analysis of Variance; Anti-Infective Agents, Local / pharmacology; Caliciviridae Infections / prevention & control; Disinfection / methods; Ethanol / pharmacology; Food Handling; Food Microbiology; Gastroenteritis / prevention & control; Hand Disinfection / methods; Humans; Infection Control; Norovirus / drug effects; Norovirus / growth & development; Viral Plaque Assay
TL;DR: It is concluded that this new ethanol-based hand sanitizer containing a synergistic blend of polyquaternium polymer and organic acid is a promising option for reducing the transmission of enteric viruses, including norovirus, by food handlers and care providers. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2008 journal article

Inactivation of bacterial pathogens in human milk by high-pressure processing

JOURNAL OF FOOD PROTECTION, 71(1), 109–118.

By: S. Viazis n, B. Farkas n & L. Jaykus n

MeSH headings : Colony Count, Microbial; Escherichia coli / drug effects; Escherichia coli / growth & development; Food Handling / methods; Food Microbiology; Humans; Hydrostatic Pressure / adverse effects; Listeria monocytogenes / drug effects; Listeria monocytogenes / growth & development; Milk Banks / standards; Milk, Human / microbiology; Nutritive Value; Peptones / pharmacology; Staphylococcus aureus / drug effects; Staphylococcus aureus / growth & development; Streptococcus agalactiae / drug effects; Streptococcus agalactiae / growth & development; Temperature; Time Factors
TL;DR: Investigation of the efficacy of high-pressure processing for inactivation of selected bacterial pathogens in human milk suggests that HPP may be a promising alternative for pasteurization of human milk. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2008 journal article

Microbial Concentrations on Fresh Produce Are Affected by Postharvest Processing, Importation, and Season

JOURNAL OF FOOD PROTECTION, 71(12), 2389–2397.

By: E. Ailes*, J. Leon, L. Jaykus*, L. Johnston*, H. Clayton, S. Blanding, D. Kleinbaum, L. Backer, C. Moe

MeSH headings : Bacteria / growth & development; Bacteria / isolation & purification; Colony Count, Microbial; Commerce; Consumer Product Safety; Disease Outbreaks / prevention & control; Enterobacteriaceae / growth & development; Enterobacteriaceae / isolation & purification; Escherichia coli / growth & development; Escherichia coli / isolation & purification; Food Contamination / analysis; Food Contamination / prevention & control; Food Handling / methods; Food Handling / standards; Food Microbiology; Food Packaging / methods; Food Packaging / standards; Food Preservation / methods; Logistic Models; Predictive Value of Tests; Seasons; United States; Vegetables / microbiology; Vegetables / standards
TL;DR: A predictive, multivariate logistic regression model was developed to identify and quantify factors that were associated with detectable concentrations of E. coli contamination on produce, which included produce type, season of collection, and packing step. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2008 journal article

Outbreak of Norovirus Infection among River Rafters Associated with Packaged Delicatessen Meat, Grand Canyon, 2005

Clinical Infectious Diseases, 48(1), 31–37.

By: M. Malek*, E. Barzilay*, A. Kramer*, B. Camp*, L. Jaykus n, B. Escudero‐Abarca n, G. Derrick*, P. White* ...

MeSH headings : Caliciviridae Infections / epidemiology; Case-Control Studies; Disease Outbreaks; Environmental Microbiology; Feces / virology; Foodborne Diseases / epidemiology; Foodborne Diseases / virology; Gastroenteritis / epidemiology; Humans; Meat Products / virology; Norovirus / isolation & purification; United States / epidemiology
TL;DR: Food handlers can contaminate ready-to-eat meats with norovirus during processing, and meat-processing practices should include specific measures to prevent contamination with enteric viruses and subsequent widespread outbreaks. (via Semantic Scholar)
Source: Crossref
Added: February 24, 2020

2008 journal article

Rapid and sensitive detection of hepatitis A virus in representative food matrices

JOURNAL OF VIROLOGICAL METHODS, 147(1), 177–187.

By: E. Papafragkou n, M. Plante*, K. Mattison*, S. Bidawid*, K. Karthikeyan*, J. Farber*, L. Jaykus n

author keywords: hepatitis A virus; food virology; viral extraction methods
MeSH headings : Animals; Cell Line; Disease Outbreaks; Food / virology; Hepatitis A / epidemiology; Hepatitis A virus / isolation & purification; Humans; Magnetics; Nucleic Acid Hybridization; Reverse Transcriptase Polymerase Chain Reaction; Sensitivity and Specificity
TL;DR: This is the first application of the automated magnetic capture technology to the concentration of viruses from foods, and it offers promise for facilitating the rapid detection of HAV from naturally contaminated products. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2007 article

Accurate identification of desired clones from 16S-23S rDNA spacer libraries using single PCR

Gonzalez-Escalona, N., Jaykus, L.-A., & DePaola, A. (2007, January 1). ANALYTICAL BIOCHEMISTRY, Vol. 360, pp. 146–147.

By: N. Gonzalez-Escalona*, L. Jaykus n & A. DePaola*

MeSH headings : Base Sequence; Cloning, Molecular; DNA Primers; DNA, Ribosomal / genetics; Electrophoresis, Polyacrylamide Gel; Polymerase Chain Reaction / methods; RNA, Ribosomal, 16S / genetics; RNA, Ribosomal, 23S / genetics
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2007 review

An overview of Vibrio vulnificus and Vibrio parahaemolyticus

[Review of ]. COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY, 6(4), 120–144.

By: S. Drake*, A. DePaola & L. Jaykus*

TL;DR: The reader will gain an understanding of why these organisms pose a public health risk and how improving understanding of their behavior in the environment and the host can aid in reducing that risk in the future. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2007 journal article

Comparison of direct genome Restriction Enzyme analysis and pulsed-field gel electrophoresis for typing of Vibrio vulnificus and their correspondence with multilocus sequence typing data

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 73(22), 7494–7500.

MeSH headings : Bacterial Typing Techniques / methods; DNA, Bacterial / genetics; DNA, Bacterial / metabolism; Electrophoresis, Gel, Pulsed-Field / methods; Genome, Bacterial / genetics; Humans; Phylogeny; Reproducibility of Results; Restriction Mapping / methods; Vibrio vulnificus / classification; Vibrio vulnificus / genetics; Vibrio vulnificus / isolation & purification
TL;DR: DGREA established clearer relationships among V. vulnificus strains and was more consistent with MLST than with PFGE, and is a very promising tool for epidemiological and ecological studies of Vibrio vulnIFICus. (via Semantic Scholar)
UN Sustainable Development Goal Categories
10. Reduced Inequalities (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2007 journal article

Evaluation of BBL CHROMagar Listeria agar for the isolation and identification of Listeria monocytogenes from food and environmental samples

JOURNAL OF MICROBIOLOGICAL METHODS, 68(1), 82–87.

By: V. Hegde*, C. Leon-Velarde, C. Stam*, L. Jaykus* & J. Odumeru

author keywords: Listeria monocytogenes; Listeria species; chromogenic agar
MeSH headings : Agar; Bacteriological Techniques / methods; Chromogenic Compounds / chemistry; Culture Media; Food Microbiology; Listeria monocytogenes / isolation & purification; Listeriosis / microbiology; Listeriosis / prevention & control; Sensitivity and Specificity
TL;DR: BBL CHROMagar Listeria offers a high degree of specificity for the confirmation of suspect L. monocytogenes colonies, whereas non-chromogenic selective agars evaluated were not differential for L.monocytogens. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2007 journal article

Feasibility of utilizing bioindicators for testing microbial inactivation in sweetpotato purees processed with a continuous-flow microwave system

JOURNAL OF FOOD SCIENCE, 72(5), E235–E242.

By: T. Brinley, C. Stam, V. Truong*, P. Coronel*, P. Kumar*, J. Simunovic*, K. Sandeep*, G. Cartwright* ...

author keywords: aseptic; bloindicators; continuous-flow; microwave; purees
MeSH headings : Bacillus / growth & development; Bacillus subtilis / growth & development; Colony Count, Microbial; Feasibility Studies; Food Contamination / analysis; Food Handling / methods; Food Microbiology; Hot Temperature; Ipomoea batatas / microbiology; Microwaves; Reproducibility of Results; Sensitivity and Specificity; Spores, Bacterial; Sterilization / methods; Time Factors
TL;DR: This study presents the first report suggesting that bioindicators such as the flexible, food-grade plastic pouches can be used for microbial validation of commercial sterilization in aseptic processing of foods using a continuous-flow microwave system. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2007 journal article

Genotypes, serotypes, and antibiotic resistance profiles of Salmonella isolated from commercial North Carolina Turkey farms

JOURNAL OF FOOD PROTECTION, 70(6), 1328–1333.

By: F. Santos n, D. D'Souza*, L. Jaykus n, P. Ferket n & B. Sheldon n

MeSH headings : Age Factors; Animals; Anti-Bacterial Agents / pharmacology; Consumer Product Safety; DNA, Bacterial / analysis; Drug Resistance, Bacterial; Drug Resistance, Multiple, Bacterial; Electrophoresis, Gel, Pulsed-Field / veterinary; Feces / microbiology; Food Contamination / prevention & control; Food Microbiology; Genotype; Humans; Microbial Sensitivity Tests; North Carolina; Phylogeny; Salmonella / classification; Salmonella / drug effects; Salmonella / genetics; Salmonella / isolation & purification; Salmonella Food Poisoning / prevention & control; Seasons; Serotyping / veterinary; Turkeys / microbiology
TL;DR: Salmonella enterica serotypes, genotypes and AbR patterns were affected by bird age but not by season or farm, and dendogram analysis indicated that sequence variability between serotypes was more significant than within serotypes. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2007 journal article

Listeriosis prevention for older adults: Effective messages and delivery methods

EDUCATIONAL GERONTOLOGY, 33(7), 587–606.

By: S. Cates*, K. Kosa*, C. Moore n, L. Jaykus n, T. Ten Eyck* & P. Cowen n

TL;DR: The need to reach older adults with information on listeriosis prevention was identified and adoption of the recommended practices was not widespread following exposure to the educational materials. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2007 journal article

Second-order modeling of variability and uncertainty in microbial hazard characterization

JOURNAL OF FOOD PROTECTION, 70(2), 363–372.

MeSH headings : Adolescent; Adult; Animals; Campylobacter jejuni / pathogenicity; Child; Child, Preschool; Colony Count, Microbial; Consumer Product Safety; Female; Food Contamination / analysis; Food Contamination / statistics & numerical data; Food Microbiology; Humans; Infant; Logistic Models; Male; Middle Aged; Models, Biological; Monte Carlo Method; Predictive Value of Tests; Risk Assessment
TL;DR: An analytical framework that permits quantitative consideration of variability and uncertainty in microbial hazard characterization is described and it is indicated that uncertainty associated with dose-response modeling has a dominating influence on the analytical outcome. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2007 journal article

Typing of Vibrio vulnificus strains by variability in their 16S-23S rRNA Intergenic Spacer regions

FOODBORNE PATHOGENS AND DISEASE, 4(3), 327–337.

By: N. Gonzalez-Escalona n, L. Jaykus n & A. DePaola*

MeSH headings : Animals; Bacterial Typing Techniques; Base Sequence; Cluster Analysis; DNA, Bacterial / analysis; DNA, Intergenic; DNA, Ribosomal Spacer / genetics; Food Contamination / analysis; Gene Amplification; Genetic Variation; Humans; Molecular Sequence Data; RNA, Ribosomal, 16S / genetics; RNA, Ribosomal, 23S / genetics; Sequence Alignment; Sequence Analysis, DNA; Shellfish / microbiology; Species Specificity; Vibrio vulnificus / classification; Vibrio vulnificus / genetics
TL;DR: The results demonstrate the feasibility of the ISR1 technique for V. vulnificus subtyping and suggest thatISR1 patterns appear to be linked to rrs sequence types and perhaps with virulence. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2006 journal article

A field study of the microbiological quality of fresh produce of domestic and Mexican origin

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 112(2), 83–95.

By: L. Johnston n, L. Jaykus n, D. Moll*, J. Anciso*, B. Mora* & C. Moe*

author keywords: produce; microbiological indicators; food safety; cantaloupe; Enterococcus; antibiotic resistance
MeSH headings : Anti-Bacterial Agents / pharmacology; Bacteria / drug effects; Bacteria / isolation & purification; Bacteria / pathogenicity; Colony Count, Microbial; Consumer Product Safety; Cucurbitaceae / microbiology; Drug Resistance, Bacterial; Enterococcus faecalis / drug effects; Enterococcus faecalis / isolation & purification; Enterococcus faecium / drug effects; Enterococcus faecium / isolation & purification; Food Contamination / analysis; Food Handling / methods; Food Handling / standards; Food Microbiology; Food Packaging / methods; Food Packaging / standards; Mexico; Spices / microbiology; Vegetables / microbiology; Vegetables / standards
TL;DR: This study does not support the assumption that domestic produce is of higher microbial quality than Mexican produce, and evaluates the prevalence of select pathogens on fresh produce, including leafy green, herbs, melons, and vegetables. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2006 journal article

Application of classification and regression trees for sensitivity analysis of the Escherichia coli O157 : H7 food safety process risk model

JOURNAL OF FOOD PROTECTION, 69(3), 609–618.

By: A. Mokhtari n, H. Frey n & L. Jaykus n

MeSH headings : Consumer Product Safety; Escherichia coli O157 / growth & development; Food Handling / methods; Food Microbiology; Food-Processing Industry / standards; Humans; Models, Biological; Regression Analysis; Risk Assessment / methods; Risk Assessment / standards; Sensitivity and Specificity; Temperature; Time Factors
TL;DR: The capabilities of classification and regression trees analysis are demonstrated as an alternative to other statistically based sensitivity analysis methods, and one that can readily address specific characteristics that are common in microbial food safety process risk models. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2006 journal article

Consumer knowledge, storage, and handling practices regarding Listeria in frankfurters and deli meats: Results of a web-based survey

JOURNAL OF FOOD PROTECTION, 69(7), 1630–1639.

By: S. Cates*, R. Morales*, S. Karns*, L. Jaykus n, K. Kosa*, T. Teneyck*, C. Moore n, P. Cowen n

MeSH headings : Adolescent; Adult; Age Distribution; Animals; Consumer Product Safety; Data Collection; Female; Food Handling / methods; Food Microbiology; Health Education; Health Knowledge, Attitudes, Practice; Health Promotion; Humans; Listeria / growth & development; Listeriosis / prevention & control; Male; Meat Products / microbiology; Middle Aged; Risk Assessment; Sex Distribution; Surveys and Questionnaires; Time Factors; United States
TL;DR: The need to develop targeted educational initiatives on listeriosis prevention is identified, with men, more-educated individuals, and individuals living in metropolitan areas were more likely to engage in risky storage practices. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2006 journal article

Consumer phase risk assessment for Listeria monocytogenes in deli meats

Risk Analysis, 26(1), 89–103.

By: H. Yang, A. Mokhtari, L. Jaykus, R. Morales, S. Cates & P. Cowen

Source: NC State University Libraries
Added: August 6, 2018

2006 journal article

Consumer-phase Salmonella enterica serovar Enteritidis risk assessment for egg-containing food products

RISK ANALYSIS, 26(3), 753–768.

By: A. Mokhtari n, C. Moore*, H. Yang*, L. Jaykus*, R. Morales, S. Cates, P. Cowen*

author keywords: consumer-phase risk assessment; domestic food handling and storage; Salmonella enterica serovar Enteritidis; sensitivity analysis; shell eggs
MeSH headings : Bacteriophage Typing; Consumer Product Safety; Cooking; Eggs; Food; Food Handling; Food Microbiology; Models, Statistical; Risk; Risk Assessment / methods; Risk Factors; Salmonella Food Poisoning / etiology; Salmonella Food Poisoning / prevention & control; Salmonella Infections / etiology; Salmonella enteritidis / pathogenicity
TL;DR: A one‐dimensional probabilistic model of the role of domestic food handling behaviors on salmonellosis risk associated with the consumption of eggs and egg‐containing foods is described and provides a science‐based approach to inform consumer risk communications regarding safe egg handling practices. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2006 journal article

Persistence of caliciviruses on enviromnental surfaces and their transfer to food

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 108(1), 84–91.

By: . DH D'Souza, A. Sair*, K. Williams n, E. Papafragkou n, J. Jean*, C. Moore n, L. Jaykus n

author keywords: norovirus; feline calicivirus; persistence; transfer; food
MeSH headings : Calicivirus, Feline / growth & development; Calicivirus, Feline / isolation & purification; Calicivirus, Feline / physiology; Consumer Product Safety; Environmental Microbiology; Equipment Contamination; Food Contamination / analysis; Food Microbiology; Humans; Norovirus / growth & development; Norovirus / isolation & purification; Norovirus / physiology; RNA, Viral / analysis; RNA, Viral / isolation & purification; Reverse Transcriptase Polymerase Chain Reaction; Stainless Steel / analysis; Time Factors; Viral Plaque Assay
TL;DR: This study confirms lengthy NoV persistence on common food preparation surfaces and their ease of transfer, confirming a potential role for environmental contamination in the propagation of viral gastroenteritis. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2006 journal article

Recovery and detection of Vibrio vulnificus during cold storage

FOOD MICROBIOLOGY, 24(6), 664–670.

By: W. Bang n, M. Drake n & L. Jaykus n

author keywords: V. vulnificus; cold storage; cell recovery
MeSH headings : Agar; Cold Temperature; Colony Count, Microbial / methods; Consumer Product Safety; Culture Media / chemistry; DNA, Bacterial / analysis; Food Microbiology; Food Preservation / methods; Gene Amplification; Humans; Polymerase Chain Reaction; RNA, Bacterial / analysis; Reverse Transcriptase Polymerase Chain Reaction; Time Factors; Vibrio vulnificus / growth & development; Vibrio vulnificus / isolation & purification
TL;DR: Different cultural techniques and molecular methods for the detection of Vibrio vulnificus during cold storage in a model broth system were compared to aid in the design of optimum methods to recover and/or detect V. vulnIFICus cells subjected to sublethal stress that might be encountered in food processing and storage. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2006 journal article

Surrogates for the study of norovirus stability and inactivation in the environment: A comparison of murine norovirus and feline calicivirus

JOURNAL OF FOOD PROTECTION, 69(11), 2761–2765.

By: J. Cannon*, E. Papafragkou n, G. Park*, J. Osborne n, L. Jaykus n & J. Vinje*

MeSH headings : Animals; Caliciviridae / growth & development; Caliciviridae Infections / epidemiology; Caliciviridae Infections / virology; Cats; Disease Models, Animal; Gastroenteritis / epidemiology; Gastroenteritis / virology; Humans; Hydrogen-Ion Concentration; Mice; Norovirus / growth & development; Species Specificity; Temperature; Time Factors
TL;DR: The genetic relatedness of MNV-1 to human NoVs combined with its ability to survive under gastric pH levels makes this virus a promising and relevant surrogate for studying environmental survival ofhuman NoVs. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2006 journal article

Upstream sample processing facilitates PCR detection of Listeria monocytogenes in mayonnaise-based ready-to-eat (RTE) salads

FOOD MICROBIOLOGY, 23(6), 584–590.

By: J. Isonhood n, M. Drake n & L. Jaykus n

author keywords: PCR; bacterial concentration; pathogen detection; Listeria; deli salad
MeSH headings : Blotting, Southern; Colony Count, Microbial; DNA, Bacterial / analysis; Food Contamination / analysis; Food Microbiology; Gene Amplification; Listeria monocytogenes / isolation & purification; Polymerase Chain Reaction / methods; Solanum tuberosum / microbiology; Time Factors
TL;DR: The two-step method reported here facilitates the production of a final sample concentrate of reduced volume and improved purity which was compatible with PCR amplification, offering further progress in efforts to reduce or eliminate cultural enrichment in an effort to speed time to results when applying molecular methods to the detection of pathogens in foods. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2006 journal article

Validation of a green fluorescent protein-labeled strain of Vibrio vulnificus for use in the evaluation of postharvest strategies for handling of raw oysters

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 72(11), 7205–7211.

By: S. Drake n, D. Elhanafi n, W. Bang n, M. Drake n, D. Green n & L. Jaykus n

MeSH headings : Animals; Biomarkers / metabolism; Colony Count, Microbial; Culture Media; Food Handling / methods; Green Fluorescent Proteins / genetics; Green Fluorescent Proteins / metabolism; Hydrogen-Ion Concentration; Ostreidae / virology; Shellfish; Temperature; Time Factors; Vibrio vulnificus / genetics; Vibrio vulnificus / growth & development; Vibrio vulnificus / metabolism
TL;DR: Ease of detection and comparability to the wild-type parent make Vv-GFP a good candidate for use in studying the behavior of V. vulnificus upon exposure to sublethal stressors that might be encountered during postharvest handling of molluscan shellfish. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2005 journal article

A field study of the microbiological quality of fresh produce

JOURNAL OF FOOD PROTECTION, 68(9), 1840–1847.

By: L. Johnston n, L. Jaykus n, D. Moll*, M. Martinez*, J. Anciso*, B. Mora*, C. Moe*

MeSH headings : Bacteria / growth & development; Bacteria / isolation & purification; Colony Count, Microbial; Consumer Product Safety; Food Contamination / analysis; Food Handling / methods; Food Handling / standards; Food Microbiology; Fruit / microbiology; Fruit / standards; Vegetables / microbiology; Vegetables / standards
TL;DR: It is demonstrated that each step from production to consumption may affect the microbial load of produce and reinforces government recommendations for ensuring a high-quality product. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2005 journal article

A simple method for the direct detection of Salmonella and Escherichia coli O157 : H7 from raw alfalfa sprouts and spent irrigation water using PCR

JOURNAL OF FOOD PROTECTION, 68(11), 2256–2263.

By: L. Johnston n, D. Elhanafi n, M. Drake n & L. Jaykus n

MeSH headings : Colony Count, Microbial; Consumer Product Safety; DNA, Bacterial / analysis; Escherichia coli O157 / isolation & purification; Food Microbiology; Medicago sativa / microbiology; Polymerase Chain Reaction / methods; Salmonella / isolation & purification; Sensitivity and Specificity; Water Microbiology
TL;DR: Progress was made toward development of a rapid, inexpensive, and sensitive method for the detection of sprout-associated pathogens that is relevant to current industrial practices and needs. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2005 journal article

Detection and identification of histamine-producing bacteria associated with harvesting and processing mahimahi and yellowfin tuna

JOURNAL OF FOOD PROTECTION, 68(8), 1676–1682.

By: D. Allen n, D. Green n, G. Bolton n, L. Jaykus n & W. Cope n

MeSH headings : Animals; Consumer Product Safety; Food Contamination / analysis; Food Handling / methods; Food Microbiology; Food-Processing Industry / methods; Food-Processing Industry / standards; Gram-Negative Bacteria / isolation & purification; Gram-Negative Bacteria / metabolism; Histamine / analysis; Histamine / biosynthesis; Humans; North Carolina; Perciformes / microbiology; Seafood / microbiology; Tuna / microbiology
TL;DR: It is revealed that gram-negative bacteria might not be solely responsible for histamine production in at-risk fish, and no seafood safety risks were found under the standard industry practices observed in this study. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2005 journal article

Effect of food matrix and cell growth on PCR-based detection of Escherichia coli O157 : H7 in ground beef

JOURNAL OF FOOD PROTECTION, 68(2), 225–232.

By: T. Taylor n, D. Elhanafi n, M. Drake n & L. Jaykus n

MeSH headings : Animals; Cattle; Colony Count, Microbial; DNA, Bacterial / analysis; Escherichia coli O157 / genetics; Escherichia coli O157 / growth & development; Escherichia coli O157 / isolation & purification; Food Contamination / analysis; Food Handling / methods; Food Microbiology; Hot Temperature; Meat Products / microbiology; Polymerase Chain Reaction / methods; Sensitivity and Specificity
TL;DR: Bacterial cell recovery and PCR detection limits were similar when comparing autoclaved ground beef and raw ground beef, but cell recoveries were highly variable for raw ground Beef samples in which E. coli O157:H7 cells were allowed to grow before processing for detection. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2005 journal article

Molecular characterization of Listeria monocytogenes of the serotype 4b complex (4b, 4d, 4e) from two turkey processing plants

FOODBORNE PATHOGENS AND DISEASE, 2(3), 192–200.

MeSH headings : Animals; DNA, Bacterial / analysis; DNA, Bacterial / genetics; Disease Reservoirs; Electrophoresis, Gel, Pulsed-Field / methods; Environmental Microbiology; Equipment Contamination; Food Contamination / analysis; Food Microbiology; Food-Processing Industry / standards; Genotype; Humans; Listeria monocytogenes / classification; Listeria monocytogenes / genetics; Listeria monocytogenes / isolation & purification; Listeriosis; Serotyping; Turkeys / microbiology
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2004 journal article

Antimicrobial resistance of Enterococcus species isolated from produce

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 70(5), 3133–3137.

By: L. Johnston n & L. Jaykus n

MeSH headings : Anti-Bacterial Agents / pharmacology; Drug Resistance, Bacterial; Enterococcus / drug effects; Enterococcus / isolation & purification; Enterococcus faecalis / drug effects; Enterococcus faecalis / isolation & purification; Enterococcus faecium / drug effects; Enterococcus faecium / isolation & purification; Food Microbiology; Fruit / microbiology; Humans; Lettuce / microbiology; Microbial Sensitivity Tests; Plants, Medicinal / microbiology; Southwestern United States
TL;DR: Enterococcus faecium strains had a much higher prevalence of resistance to ciprofloxacin, tetracycline, and nitrofurantoin than E. faecalis strains, and thirty-four percent of the isolate had multiple-drug-resistance patterns, excluding intrinsic resistance. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2004 review

Bacterial separation and concentration from complex sample matrices: A review

[Review of ]. CRITICAL REVIEWS IN MICROBIOLOGY, 30(1), 7–24.

By: K. Stevens n & L. Jaykus n

MeSH headings : Bacteria / growth & development; Bacteria / isolation & purification; Bacteriological Techniques / methods; Colony Count, Microbial; Food Microbiology
TL;DR: The purpose of this review is to provide a detailed understanding of the science, possibilities, and limitations of separating and concentrating bacterial cells from the food matrix in an effort to further improve the ability to harness molecular methods for the rapid detection of foodborne pathogens. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2004 journal article

Diagnosis of Norwalk virus infection by indirect enzyme immunoassay detection of salivary antibodies to recombinant Norwalk virus antigen

CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, 11(6), 1028–1034.

By: C. Moe n, A. Sair n, L. Lindesmith n, M. Estes n & L. Jaykus n

MeSH headings : Adult; Antibodies, Viral / analysis; Caliciviridae Infections / diagnosis; Capsid Proteins / immunology; Enzyme-Linked Immunosorbent Assay; Female; Humans; Immunoglobulin A / analysis; Immunoglobulin G / analysis; Male; Norwalk virus / immunology; Predictive Value of Tests; Saliva / immunology; Saliva / virology
TL;DR: The application of this EIA to an elementary school outbreak indicated that 67% of the subjects with confirmed infections had >4-fold rises in anti-NoV IgA when an antigen in the same genetic cluster as the outbreak virus was used. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2004 journal article

Direct detection of bacterial pathogens in representative dairy products using a combined bacterial concentration-PCR approach

JOURNAL OF APPLIED MICROBIOLOGY, 97(6), 1115–1122.

By: K. Stevens n & L. Jaykus n

author keywords: PCR; bacterial concentration; pathogen detection Listeria; Salmonella
MeSH headings : Blotting, Southern / methods; Cheese / microbiology; Colony Count, Microbial / methods; Culture Media; DNA, Bacterial / analysis; Dairy Products / microbiology; Dimethyl Sulfoxide / pharmacology; Dithiothreitol / pharmacology; Food Microbiology; Glycerol / pharmacology; Listeria monocytogenes / isolation & purification; Nucleic Acid Hybridization / drug effects; Polyethylene Glycols / pharmacology; Polymerase Chain Reaction / methods; Salmonella enteritidis / isolation & purification; Solvents / pharmacology; Yogurt / microbiology
TL;DR: This work aims to develop a simple, rapid method to concentrate and purify bacteria and their nucleic acids from complex dairy food matrices in preparation for direct pathogen detection using polymerase chain reaction (PCR). (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2004 journal article

Epidemic clone I-specific genetic markers in strains of Listeria monocytogenes serotype 4b from foods

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 70(7), 4158–4164.

By: S. Yildirim n, W. Lin*, A. Hitchins*, L. Jaykus n, E. Altermann n, T. Klaenhammer n, S. Kathariou n

MeSH headings : Base Composition; DNA, Bacterial / analysis; Food Microbiology; Genetic Markers; Listeria monocytogenes / classification; Listeria monocytogenes / genetics; Serotyping
TL;DR: Examination of 34 serogroup 4 (putative or confirmed serotype 4b) isolates of L. monocytogenes obtained from various foods and food-processing environments, without known implication in illness, revealed that many of these strains had methylation of cytosines at GATC sites in the genome, rendering their DNA resistant to digestion by the restriction endonuclease Sau3AI. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2004 article

Epidemic clone I-specific genetic markers in strains of Listeria monocytogenes serotype 4b from foods (vol 70, pg 4158, 2004)

Yildirim, S., Lin, W., Hitchins, A. D., Jaykus, L. A., Altermann, E., Klaenhammer, T. R., & Kathariou, S. (2004, December). APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Vol. 70, pp. 7581–7581.

By: S. Yildirim n, W. Lin n, A. Hitchins n, L. Jaykus n, E. Altermann n, T. Klaenhammer*, S. Kathariou n

Source: Web Of Science
Added: August 6, 2018

2004 journal article

Managing food safety: A systematic approach

Food Technology, 58(10), 36–39.

By: L. Jaykus, G. Acuff, F. Busta, J. Dickson, C. Hollingsworth, J. Marcy, A. McNamara

Source: NC State University Libraries
Added: August 6, 2018

2004 journal article

Multiplex nucleic acid sequence-based amplification for simultaneous detection of several enteric viruses in model ready-to-eat foods

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 70(11), 6603–6610.

By: J. Jean n, . DH D'Souza & L. Jaykus n

MeSH headings : Animals; Food Contamination; Hepatitis A virus / genetics; Hepatitis A virus / isolation & purification; Humans; Lettuce / microbiology; Meat Products / microbiology; Norovirus / genetics; Norovirus / isolation & purification; Polymerase Chain Reaction; Self-Sustained Sequence Replication / methods
TL;DR: The multiplex NASBA system provides rapid and simultaneous detection of clinically relevant food-borne viruses in a single reaction tube and may be a promising alternative to reverse transcription-PCR for the detection of viral contamination of foods. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2003 journal article

Challenges to developing real-time methods to detect pathogens in foods

ASM News, 69(7), 341–347.

By: L. Jaykus

Source: NC State University Libraries
Added: August 6, 2018

2003 journal article

Detection of Listeria monocytogenes from a model food by fluorescence resonance energy transfer-based PCR with an asymmetric fluorogenic probe set

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 69(2), 1082–1088.

By: K. Koo n & L. Jaykus n

MeSH headings : Animals; Bacterial Proteins / genetics; Bacterial Toxins; Culture Media; DNA Probes; Fluorescence Resonance Energy Transfer; Fluorescent Dyes; Heat-Shock Proteins / genetics; Hemolysin Proteins; Listeria monocytogenes / growth & development; Listeria monocytogenes / isolation & purification; Milk / microbiology; Polymerase Chain Reaction; Sensitivity and Specificity
TL;DR: This study demonstrated that this asymmetric FRET-based amplification and detection protocol provides an alternative approach for endpoint detection of nucleic acid amplification products as applied to detection of pathogens in a model food. (via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2003 journal article

Nucleic acid sequence based amplification for the rapid and sensitive detection of Salmonella enterica from foods

JOURNAL OF APPLIED MICROBIOLOGY, 95(6), 1343–1350.

By: . DH D'Souza & L. Jaykus*

author keywords: detection; foods; NASBA; Salmonella enterica
MeSH headings : Bacteriological Techniques; Colony Count, Microbial; Feasibility Studies; Food Microbiology; Humans; Nucleic Acid Amplification Techniques / methods; RNA, Bacterial / isolation & purification; Salmonella enteritidis / isolation & purification
TL;DR: The purpose of this study was to apply nucleic acid sequence‐based amplification (NASBA) for the detection of Salmonella enterica serovar Enteritidis in representative foods. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2003 journal article

Survival and persistence of Campylobacter and Salmonella species under various organic loads on food contact surfaces

JOURNAL OF FOOD PROTECTION, 66(9), 1587–1594.

By: A. De Cesare n, B. Sheldon n, K. Smith n & L. Jaykus n

MeSH headings : Animals; Campylobacter / growth & development; Campylobacter / isolation & purification; Chickens / microbiology; Colony Count, Microbial; Culture Media; Equipment Contamination; Food Contamination / analysis; Food Handling / methods; Food Microbiology; Kinetics; Poultry Products / microbiology; Salmonella / growth & development; Salmonella / isolation & purification; Temperature
TL;DR: Findings indicate that both the contact surface and the level of organic matter can influence the survival and persistence of C. jejuni and Salmonella species on food contact surfaces. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2003 journal article

Transcriptional enhancement of RT-PCR for rapid and sensitive detection of Noroviruses

FEMS MICROBIOLOGY LETTERS, 226(2), 339–345.

By: J. Jean n, . D D'Souza & L. Jaykus n

author keywords: Norovirus; Norwalk-like virus; enteric virus; RT-PCR; transcriptional enhancement
MeSH headings : Environmental Microbiology; Food Microbiology; Norovirus / genetics; Norovirus / isolation & purification; Nucleic Acid Hybridization; Reproducibility of Results; Reverse Transcriptase Polymerase Chain Reaction / methods; Self-Sustained Sequence Replication / methods; Sensitivity and Specificity; Transcription, Genetic
TL;DR: The NASBA-derived primers performed comparably to other broadly reactive GII Norovirus primers with respect to detection limits and shows promise for improved detection sensitivity in instances where very low levels of virus contamination might be anticipated. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2003 journal article

Transfer of Salmonella and Campylobacter from stainless steel to romaine lettuce

JOURNAL OF FOOD PROTECTION, 66(12), 2231–2236.

By: C. Moore n, B. Sheldon n & L. Jaykus n

MeSH headings : Bacterial Adhesion; Campylobacter jejuni / growth & development; Campylobacter jejuni / physiology; Colony Count, Microbial; Equipment Contamination; Food Contamination; Food Microbiology; Lettuce / microbiology; Salmonella typhimurium / growth & development; Salmonella typhimurium / physiology; Stainless Steel
TL;DR: It is indicated that relatively high numbers of bacteria may be transferred to a food even 1 to 2 h after surface contamination, and can be used to support future projects aimed at estimating the degree of risk associated with poor handling practices of ready-to-eat foods. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2002 journal article

Detection of single nucleotide polymorphisms within the Listeria genus using an 'asymmetric' fluorogenic probe set and fluorescence resonance energy transfer based-PCR

LETTERS IN APPLIED MICROBIOLOGY, 35(6), 513–517.

By: K. Koo n & L. Jaykus n

MeSH headings : Fluorescence Resonance Energy Transfer / methods; Fluorescent Dyes; Listeria / classification; Listeria / genetics; Listeria monocytogenes / classification; Listeria monocytogenes / genetics; Polymerase Chain Reaction / methods; Polymorphism, Single Nucleotide / genetics; RNA, Ribosomal, 16S / genetics; Species Specificity
TL;DR: A novel and inexpensive fluorescence energy transfer (FRET)‐based PCR protocol to distinguish single nucleotide polymorphisms (SNPs) within the genus Listeria is described. (via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2002 journal article

Improved detection of human enteric viruses in foods by RT-PCR

JOURNAL OF VIROLOGICAL METHODS, 100(1-2), 57–69.

By: A. Sair n, . DH D'Souza, C. Moe* & L. Jaykus n

author keywords: Norwalk virus; hepatitis A virus; RNA purity; RT-PCR; foods
MeSH headings : DNA Primers; Food Microbiology; Hepatitis A virus / genetics; Hepatitis A virus / isolation & purification; Humans; Norwalk virus / genetics; Norwalk virus / isolation & purification; RNA, Viral / analysis; Reverse Transcriptase Polymerase Chain Reaction
TL;DR: The methods reported here show promise for rapid, sensitive detection of human enteric viruses in foods as evidenced by the ability to detect viral RNA in food concentrates without prior dilution. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2002 journal article

Influence of growth in a food medium on the detection of Escherichia coli O157 : H7 by polymerase chain reaction

JOURNAL OF FOOD PROTECTION, 65(11), 1775–1779.

By: J. McKillip*, L. Jaykus* & M. Drake*

MeSH headings : Colony Count, Microbial; Culture Media; DNA, Bacterial / analysis; Escherichia coli O157 / genetics; Escherichia coli O157 / growth & development; Escherichia coli O157 / isolation & purification; Food Contamination / analysis; Food Microbiology; Polymerase Chain Reaction / methods; Sensitivity and Specificity; Time Factors
TL;DR: The results of this investigation underscore the likelihood that multiple components may drastically affect DNA extraction and PCR amplification efficiency in the detection of pathogens in the food matrix and it would be prudent to test their efficacy in multiple food matrices and under conditions in which the bacterial population is both static and actively growing. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2002 journal article

Magnetized carbonyl iron and insoluble zirconium hydroxide mixture facilitates bacterial concentration and separation from nonfat dry milk

JOURNAL OF FOOD PROTECTION, 65(11), 1806–1810.

By: M. Cullison n & L. Jaykus n

MeSH headings : Animals; Bacillus cereus / isolation & purification; Cells, Immobilized; Colony Count, Microbial; Food Microbiology; Iron Carbonyl Compounds; Listeria monocytogenes / isolation & purification; Milk / microbiology; Organometallic Compounds; Salmonella enteritidis / isolation & purification; Sensitivity and Specificity; Solubility; Spores, Bacterial; Zirconium
TL;DR: The entire concentration scheme resulted in a 25-fold reduction in sample volume with the recovery of viable bacterial cells, which shows promise for facilitating inexpensive, rapid, and effective bacterial concentration in food systems. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2002 journal article

Sensitivity analysis of Salmonella enteritidis levels in contaminated shell eggs using a biphasic growth model

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 75(1-2), 71–87.

By: H. Latimer*, L. Jaykus n, R. Morales*, P. Cowen n & D. Crawford-Brown*

author keywords: salmonella enteritidis; shell eggs; microbial risk assessment; food safety
MeSH headings : Animals; Chickens; Consumer Product Safety; Egg Yolk / microbiology; Eggs / microbiology; Food Contamination; Food Handling / methods; Food Microbiology; Models, Biological; Monte Carlo Method; Salmonella enteritidis / growth & development; Seasons; Temperature
TL;DR: The results of this study suggest that, for maximum reduction of SE exposure level, cooling strategies should not only focus on the on-farm or processing phases, but should emphasize the importance of cooling strategies at the distribution and consumer phases of the farm-to-fork continuum. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2002 journal article

Zirconium hydroxide effectively immobilizes and concentrates human enteric viruses

LETTERS IN APPLIED MICROBIOLOGY, 35(5), 414–418.

By: . DH D'Souza & L. Jaykus n

MeSH headings : Hepatitis A virus / growth & development; Hepatitis A virus / isolation & purification; Humans; Hydroxides / pharmacology; Norwalk virus / growth & development; Norwalk virus / isolation & purification; Poliovirus / growth & development; Poliovirus / isolation & purification; Polymerase Chain Reaction; Virus Cultivation / methods; Zirconium / pharmacology
TL;DR: This study uses the use of zirconium hydroxide to concentrate bacteria from clinical, environmental, and food samples to detection of human enteric viruses in foods and environmental samples. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2001 journal article

A weighted composite dose-response model for human salmonellosis

RISK ANALYSIS, 21(2), 295–305.

By: H. Latimer*, L. Jaykus*, R. Morales, P. Cowen* & D. Crawford-Brown

author keywords: Salmonella enteritidis; weighted composite dose-response relationships; maximum-likelihood estimation; degree of model confidence; likelihood ratios
MeSH headings : Colony Count, Microbial; Food Microbiology; Humans; Likelihood Functions; Models, Biological; Risk; Salmonella / pathogenicity; Salmonella Food Poisoning / etiology; Salmonella enteritidis / pathogenicity; Virulence
TL;DR: The influence of variation in strain virulence and host susceptibility on the shape of the population dose – response relationship is described. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2000 journal article

A comparison of methods for the detection of Escherichia coli O157 : H7 from artificially-contaminated dairy products using PCR

JOURNAL OF APPLIED MICROBIOLOGY, 89(1), 49–55.

By: J. McKillip*, L. Jaykus n & M. Drake*

MeSH headings : Animals; Cheese / microbiology; DNA, Bacterial / analysis; DNA, Bacterial / isolation & purification; Escherichia coli O157 / genetics; Escherichia coli O157 / isolation & purification; Food Microbiology; Lactose / pharmacology; Milk / microbiology; Milk Proteins; Polymerase Chain Reaction; Whey Proteins
TL;DR: It is concluded that the efficiency of DNA extraction is an extremely important and frequently overlooked variable impacting the overall detection limits of PCR‐based detection strategies. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018