Matthew K. Shipton

MeSH headings : Amino Acid Sequence; Endocytosis; Gold / chemistry; HeLa Cells; Humans; Molecular Structure; Molecular Weight; Nanoparticles / chemistry; Peptides / chemical synthesis; Peptides / chemistry; Polyethylene Glycols / chemistry; Spectrometry, Fluorescence; Sulfhydryl Compounds / chemistry

TL;DR: The assembly of poly(ethylene glycol) (PEG) and mixed peptide/PEG monolayers on gold nanoparticle surfaces is described to provide useful cues in the assembly of stable peptides/gold nanoparticle bioconjugates capable of being internalized into cells. (via Semantic Scholar)

MeSH headings : Base Sequence; Gold / chemistry; In Vitro Techniques; Metal Nanoparticles / chemistry; Metal Nanoparticles / ultrastructure; Microscopy, Electron, Scanning; Nucleic Acid Conformation; Palladium / chemistry; RNA, Catalytic / chemistry; RNA, Catalytic / metabolism; Surface Properties

TL;DR: The use of surface-bound RNA as a tool for directing the orthogonal synthesis of materials on surfaces was demonstrated and it was suggested that a single RNA molecule may be sufficient for nucleating and controlling the shapes of these particles. (via Semantic Scholar)

MeSH headings : 3T3 Cells; Animals; Biological Transport, Active / physiology; Cell Line, Tumor; Cell Nucleus / drug effects; Cell Nucleus / metabolism; Gene Products, tat / chemistry; Gold / chemistry; Gold / pharmacokinetics; HeLa Cells; Humans; Macromolecular Substances; Mice; Nuclear Localization Signals / drug effects; Nuclear Localization Signals / metabolism; Peptides / chemistry; Peptides / pharmacokinetics; Protein Structure, Tertiary / physiology; Serum Albumin, Bovine / chemistry; Serum Albumin, Bovine / pharmacokinetics; Temperature; Transduction, Genetic

TL;DR: Video-enhanced color differential interference contrast microscopy and transmission electron microscopy indicated that transport of nanoparticles into the cytoplasm and nucleus depends on peptide sequence and cell line, and cellular uptake of Tat PTD peptide-gold nanoparticle conjugates was temperature dependent, suggesting an endosomal pathway of uptake. (via Semantic Scholar)