Alice M. Lee

Works (4)

Updated: July 5th, 2023 15:56

2009 journal article

Expression of Pyrococcus furiosus Superoxide Reductase in Arabidopsis Enhances Heat Tolerance

PLANT PHYSIOLOGY, 151(2), 893–904.

By: Y. Im n, M. Ji n, A. Lee n, R. Killens n, A. Grunden n  & W. Boss n

MeSH headings : Adaptation, Physiological; Arabidopsis / genetics; Arabidopsis / metabolism; Arabidopsis Proteins / genetics; Arabidopsis Proteins / metabolism; Ascorbate Peroxidases; Chlorophyll / metabolism; Gene Expression Regulation, Plant; Germination; Heat-Shock Response; Hot Temperature; Hydrogen Peroxide / metabolism; Immunoprecipitation; Oxidoreductases / genetics; Oxidoreductases / metabolism; Peroxidases / metabolism; Plant Extracts / metabolism; Plant Leaves / metabolism; Plant Roots / metabolism; Plants, Genetically Modified; Pyrococcus furiosus / enzymology; RNA, Messenger / genetics; RNA, Messenger / metabolism; Seedlings / enzymology; Seeds / metabolism
TL;DR: It is shown that P. furiosus SOR can be produced as a functional enzyme in planta and that plants producing SOR have enhanced tolerance to heat, light, and chemically induced ROS. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2009 journal article

Proteomics of Pyrococcus furiosus, a Hyperthermophilic Archaeon Refractory to Traditional Methods

JOURNAL OF PROTEOME RESEARCH, 8(8), 3844–3851.

By: A. Lee n, J. Sevinsky  n, J. Bundy n, A. Grunden n  & J. Stephenson n

author keywords: Pyrococcus furiosus; shotgun proteomics; archaea; hyperthermophile; high throughput; IPG-IEF; LC-MS/MS; archaeal proteins; microwave assisted acid hydrolysis
MeSH headings : Archaeal Proteins / analysis; Archaeal Proteins / metabolism; Hydrogen-Ion Concentration; Hydrolysis; Isoelectric Point; Microwaves; Peptide Fragments / analysis; Peptide Fragments / metabolism; Peptide Mapping; Protein Conformation; Proteome / analysis; Proteome / metabolism; Proteomics / methods; Pyrococcus furiosus / chemistry; Trypsin / metabolism
TL;DR: A simple shotgun proteomics approach for the global identification of the P. furiosus proteome using a detergent-based microwave assisted acid hydrolysis step coupled with an overnight trypsin digest to obtain peptides is developed. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2007 journal article

Warp breaks detection in Jacquard weaving using MEMS: System development

JOURNAL OF THE TEXTILE INSTITUTE, 98(3), 275–280.

By: J. Lee n, A. Seyam n , G. Hodge n , W. Oxenham n & E. Grant n

Contributors: J. Lee n, A. Seyam  n, G. Hodge n , W. Oxenham n & E. Grant n

author keywords: MEMS accelerometers; Jacquard weaving; warp breaks; automation of warp breaks
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2005 journal article

Production of a thermostable archaeal superoxide reductase in plant cells

FEBS LETTERS, 579(25), 5521–5526.

By: Y. Im n, M. Ji n, A. Lee n, W. Boss n & A. Grunden n 

author keywords: archaeal; heat-stress; hyperthermophile; plant; reactive oxygen species; superoxide dismutase; superoxide reductase
MeSH headings : Archaeal Proteins / analysis; Archaeal Proteins / biosynthesis; Archaeal Proteins / genetics; Cells, Cultured; Cytoplasm / chemistry; Enzyme Stability; Green Fluorescent Proteins / analysis; Green Fluorescent Proteins / biosynthesis; Hot Temperature; Oxidoreductases / analysis; Oxidoreductases / biosynthesis; Oxidoreductases / genetics; Plants, Genetically Modified / cytology; Plants, Genetically Modified / genetics; Plants, Genetically Modified / metabolism; Pyrococcus furiosus / enzymology; Recombinant Fusion Proteins / analysis; Recombinant Fusion Proteins / biosynthesis; Superoxides / metabolism; Tobacco / cytology; Tobacco / genetics; Tobacco / metabolism
TL;DR: The Archaeal SOR provides a novel mechanism to reduce superoxide and demonstrates the potential for using archaeal genes to alter eukaryotic metabolism. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

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