Works (5)

Updated: July 5th, 2023 15:56

2016 journal article

Enhancing Heat Tolerance of the Little Dogwood Cornus canadensis L. f. with Introduction of a Superoxide Reductase Gene from the Hyperthermophilic Archaeon Pyrococcus furiosus

Frontiers in Plant Science, 7.

By: X. Geng n, X. Liu n, M. Ji n, W. Hoffmann n, A. Grunden n & Q. Xiang n

author keywords: antioxidant enzyme; Cornus canadensis; genetic transformation; heat tolerance; Pyrococcus furiosus; reactive oxygen species (ROS); superoxide reductase (SOR)
TL;DR: This is the first report of the successful demonstration of improved heat tolerance in a non-model plant resulting from the introduction of P. furiosus SOR, and demonstrates the potential of SOR for crop improvement and that inherent limitations of plant heat tolerance can be ameliorated with P.furiosu SOR. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2015 journal article

A novel gateway-compatible binary vector series (PC-GW) for flexible cloning of multiple genes for genetic transformation of plants

PLASMID, 81, 55–62.

By: J. Dalal n, R. Yalamanchili n, C. Hovary n, M. Ji n, M. Rodriguez-Welsh n, D. Aslett n, S. Ganapathy n, A. Grunden n ...

Contributors: J. Dalal n, R. Yalamanchili n, C. La Hovary n, M. Ji n, M. Rodriguez-Welsh n, D. Aslett n, S. Ganapathy n, A. Grunden n, H. Sederoff n, R. Qu n

author keywords: Gateway; Plant transformation; Agrobacterium; Gene stacking
MeSH headings : Cloning, Molecular / methods; Gene Expression; Gene Order; Genes, Reporter; Genetic Vectors / genetics; Plants, Genetically Modified; Plasmids / genetics; Transformation, Genetic; Transgenes
TL;DR: The PC-GW vectors provide choices for selectable markers, cloning methods, and can accommodate up to eight gene constructs in a single T-DNA, thereby significantly reducing the number of transformations or crosses needed to generate multi-transgene expressing plants. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2015 journal article

Characterization of recombinant glutathione reductase from the psychrophilic Antarctic bacterium Colwellia psychrerythraea

EXTREMOPHILES, 19(4), 863–874.

By: M. Ji n, C. Barnwell n & A. Grunden n

author keywords: Colwellia psychrerythraea; Glutathione reductase; Psychrophile; Oxidative stress; Antioxidant enzyme
MeSH headings : Alteromonadaceae / enzymology; Alteromonadaceae / genetics; Antarctic Regions; Bacterial Proteins / biosynthesis; Bacterial Proteins / chemistry; Bacterial Proteins / genetics; Glutathione Reductase / biosynthesis; Glutathione Reductase / chemistry; Glutathione Reductase / genetics; Recombinant Proteins / biosynthesis; Recombinant Proteins / chemistry; Recombinant Proteins / genetics
TL;DR: A recombinant form of glutathione reductase from Colwellia psychrerythraea, a marine psychrophilic bacterium, has been biochemically characterized to determine its molecular and enzymatic properties, suggesting its potential utility as an antioxidant enzyme in heterologous systems. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2009 journal article

Expression of Pyrococcus furiosus Superoxide Reductase in Arabidopsis Enhances Heat Tolerance

PLANT PHYSIOLOGY, 151(2), 893–904.

By: Y. Im n, M. Ji n, A. Lee n, R. Killens n, A. Grunden n & W. Boss n

MeSH headings : Adaptation, Physiological; Arabidopsis / genetics; Arabidopsis / metabolism; Arabidopsis Proteins / genetics; Arabidopsis Proteins / metabolism; Ascorbate Peroxidases; Chlorophyll / metabolism; Gene Expression Regulation, Plant; Germination; Heat-Shock Response; Hot Temperature; Hydrogen Peroxide / metabolism; Immunoprecipitation; Oxidoreductases / genetics; Oxidoreductases / metabolism; Peroxidases / metabolism; Plant Extracts / metabolism; Plant Leaves / metabolism; Plant Roots / metabolism; Plants, Genetically Modified; Pyrococcus furiosus / enzymology; RNA, Messenger / genetics; RNA, Messenger / metabolism; Seedlings / enzymology; Seeds / metabolism
TL;DR: It is shown that P. furiosus SOR can be produced as a functional enzyme in planta and that plants producing SOR have enhanced tolerance to heat, light, and chemically induced ROS. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2005 journal article

Production of a thermostable archaeal superoxide reductase in plant cells

FEBS LETTERS, 579(25), 5521–5526.

By: Y. Im n, M. Ji n, A. Lee n, W. Boss n & A. Grunden n

author keywords: archaeal; heat-stress; hyperthermophile; plant; reactive oxygen species; superoxide dismutase; superoxide reductase
MeSH headings : Archaeal Proteins / analysis; Archaeal Proteins / biosynthesis; Archaeal Proteins / genetics; Cells, Cultured; Cytoplasm / chemistry; Enzyme Stability; Green Fluorescent Proteins / analysis; Green Fluorescent Proteins / biosynthesis; Hot Temperature; Oxidoreductases / analysis; Oxidoreductases / biosynthesis; Oxidoreductases / genetics; Plants, Genetically Modified / cytology; Plants, Genetically Modified / genetics; Plants, Genetically Modified / metabolism; Pyrococcus furiosus / enzymology; Recombinant Fusion Proteins / analysis; Recombinant Fusion Proteins / biosynthesis; Superoxides / metabolism; Tobacco / cytology; Tobacco / genetics; Tobacco / metabolism
TL;DR: The Archaeal SOR provides a novel mechanism to reduce superoxide and demonstrates the potential for using archaeal genes to alter eukaryotic metabolism. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

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