Works (5)
Updated: July 5th, 2023 15:55
2015 article
An autophosphorylation site database for leucine‐rich repeat receptor‐like kinases in Arabidopsis thaliana
Mitra, S. K., Chen, R., Dhandaydham, M., Wang, X., Blackburn, R. K., Kota, U., … Clouse, S. D. (2015, April 24). The Plant Journal.
author keywords: receptor kinase; phosphorylation; Arabidopsis thaliana; mass spectrometry; motif analysis
MeSH headings : Amino Acid Motifs; Amino Acid Sequence; Arabidopsis / genetics; Arabidopsis / metabolism; Arabidopsis Proteins / genetics; Arabidopsis Proteins / metabolism; Cytoplasm / metabolism; Databases, Factual; Escherichia coli / genetics; Molecular Sequence Data; Phosphorylation; Protein Kinases / genetics; Protein Kinases / metabolism; Protein Serine-Threonine Kinases / genetics; Protein Serine-Threonine Kinases / metabolism; Protein Structure, Tertiary
topics (OpenAlex): Plant-Microbe Interactions and Immunity; Plant Reproductive Biology; Plant Stress Responses and Tolerance
TL;DR:
This study employed high-throughput cloning and a variety of mass spectrometry approaches to generate an autophosphorylation site database representative of more than 30% of the approximately 223 LRR RLKs in Arabidopsis thaliana, and used this database to analyze trends in the localization of phosphorylation sites across cytoplasmic kinase subdomains.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2013 article
Identification and Functional Analysis of Tomato BRI1 and BAK1 Receptor Kinase Phosphorylation Sites
Bajwa, V. S., Wang, X., Blackburn, R. K., Goshe, M. B., Mitra, S. K., Williams, E. L., … Clouse, S. D. (2013, July 10). PLANT PHYSIOLOGY.
MeSH headings : Binding Sites; Kinetics; Solanum lycopersicum / enzymology; Phosphorylation; Plant Proteins / chemistry; Plant Proteins / metabolism; Protein Serine-Threonine Kinases / chemistry; Protein Serine-Threonine Kinases / metabolism; Signal Transduction
topics (OpenAlex): Plant Reproductive Biology; Plant Molecular Biology Research; Polysaccharides and Plant Cell Walls
TL;DR:
Liquid chromatography-tandem mass spectrometry was used to identify multiple in vitro phosphorylation sites of the tomato BRI1 and BAK1 cytoplasmic domains and found that tomato TRIP1 is a substrate of both tomato Bri1 andBAK1 kinases in vitro.
(via Semantic Scholar)
open access via www.plantphysiol.org (publisher PDF)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2010 article
Improving Protein and Proteome Coverage through Data-Independent Multiplexed Peptide Fragmentation
Blackburn, K., Mbeunkui, F., Mitra, S. K., Mentzel, T., & Goshe, M. B. (2010, May 7). Journal of Proteome Research.
author keywords: Proteomics; mass spectrometry; protein characterization; data-independent acquisition (DIA); data-dependent acquisition (DDA); protein identification; LC/MSE
MeSH headings : Amino Acid Sequence; Animals; Arabidopsis Proteins / chemistry; Arabidopsis Proteins / metabolism; Artifacts; Cattle; Chromatography, Liquid / methods; Ions / chemistry; Solanum lycopersicum; Mass Spectrometry / methods; Molecular Sequence Data; Peptide Fragments / chemistry; Peptide Fragments / metabolism; Plant Leaves; Plant Proteins / chemistry; Plant Proteins / metabolism; Protein Kinases / chemistry; Protein Kinases / metabolism; Proteomics / methods; Serum Albumin, Bovine / chemistry; Serum Albumin, Bovine / metabolism; Trypsin / metabolism
topics (OpenAlex): Advanced Proteomics Techniques and Applications; Mass Spectrometry Techniques and Applications; Identification and Quantification in Food
TL;DR:
The fundamental limitations of serial MS/MS interrogation are revealed and the advantages of parallel fragmentation by DIA for more comprehensive protein identification and characterization are revealed which holds promise for enhanced isoform and post-translational modification analysis.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2009 article
An Efficient Organic Solvent Based Extraction Method for the Proteomic Analysis ofArabidopsisPlasma Membranes
Mitra, S. K., Walters, B. T., Clouse, S. D., & Goshe, M. B. (2009, April 1). Journal of Proteome Research.
author keywords: Proteomics; mass spectrometry; liquid chromatography; strong cation exchanges; phase partitioning; membrane proteins; transporters; Arabidopsis; methanol
MeSH headings : Amino Acid Sequence; Arabidopsis / chemistry; Arabidopsis Proteins / chemistry; Arabidopsis Proteins / isolation & purification; Arabidopsis Proteins / metabolism; Chemical Fractionation / methods; Chloroform; Chlorophyll; Chromatography, Ion Exchange; Databases, Protein; Membrane Proteins / chemistry; Membrane Proteins / isolation & purification; Membrane Proteins / metabolism; Methanol; Molecular Sequence Data; Peptide Fragments / chemistry; Peptide Fragments / metabolism; Protein Kinases / isolation & purification; Protein Kinases / metabolism; Proteomics / methods; Solvents / chemistry; Tandem Mass Spectrometry; Trypsin / metabolism
topics (OpenAlex): Advanced Proteomics Techniques and Applications; Photosynthetic Processes and Mechanisms; Plant Reproductive Biology
TL;DR:
The combined use of methanol and chloroform provides an effective method to study membrane proteins and can be readily applied to other tissues and cells types for proteomic analysis.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2007 article
Membrane Proteomic Analysis of Arabidopsis thaliana Using Alternative Solubilization Techniques
Mitra, S. K., Gantt, J. A., Ruby, J. F., Clouse, S. D., & Goshe, M. B. (2007, April 14). Journal of Proteome Research.
author keywords: arabidopsis; proteomics; liquid chromatography; mass spectrometry; membrane proteins; phosphorylation; leucine-rich repeat receptor-like kinases
MeSH headings : Amino Acid Sequence; Arabidopsis / chemistry; Arabidopsis / cytology; Arabidopsis Proteins / analysis; Arabidopsis Proteins / genetics; Arabidopsis Proteins / isolation & purification; Bacteriorhodopsins / analysis; Bacteriorhodopsins / genetics; Bacteriorhodopsins / isolation & purification; Cetomacrogol / chemistry; Chromatography, Liquid / methods; Cytochromes c / analysis; Cytochromes c / genetics; Cytochromes c / isolation & purification; Membrane Proteins / analysis; Membrane Proteins / genetics; Membrane Proteins / isolation & purification; Methanol / chemistry; Molecular Sequence Data; Peptides / analysis; Peptides / chemistry; Peptides / genetics; Proteome / analysis; Solubility; Surface-Active Agents / chemistry; Tandem Mass Spectrometry / methods
topics (OpenAlex): Photosynthetic Processes and Mechanisms; Photoreceptor and optogenetics research; Mitochondrial Function and Pathology
TL;DR:
Overall, the data show that methanol is a better alternative for identifying a wider range of membrane proteins than the nonionic detergent Brij-58.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018