William T. Steede

Works (9)

Updated: June 29th, 2024 05:01

2024 article

Engineering Sclareol Production on the Leaf Surface of <i>Nicotiana tabacum</i>

Ma, H., Steede, T., Dewey, R. E., & Lewis, R. S. (2024, June 6). JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, Vol. 6.

By: H. Ma n, T. Steede n, R. Dewey n & R. Lewis n 

author keywords: sclareol; terpene; terpenoid; metabolicengineering; tobacco; trichome; diterpenoid; leaf surface; secondary metabolite
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: June 24, 2024

2022 journal article

Analyses of diverse low alkaloid tobacco germplasm identify naturally occurring nucleotide variability contributing to reduced leaf nicotine accumulation

MOLECULAR BREEDING, 42(1).

By: N. Burner  n, A. McCauley n, S. Pramod *, J. Frederick *, T. Steede n, S. Kernodle n, R. Lewis n 

author keywords: Nicotiana tabacum; Tobacco; Nicotine alkaloids; Transcription factors; QTL mapping
TL;DR: P phenotyped a series of 81 selected diverse tobacco introductions to identify a sub-group with authentic low alkaloid phenotypes and found a newly discovered 5 bp deletion in the gene MYC2a on linkage group 5 was found to likely partially underlie the ultra-low alkaloids phenotype of TI 313. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: January 24, 2022

2022 article

Combined reduced expression of two gene families lowers nicotine content to ultra-low levels in cultivated tobacco

Kernodle, S. P., Webb, S., Steede, T. M., & Lewis, R. S. (2022, July 2). PLANT CELL REPORTS, Vol. 7.

By: S. Kernodle n, S. Webb n, T. Steede n & R. Lewis n 

author keywords: Tobacco; Nicotiana tabacum; Nicotine; Alkaloids; RNA interference; MPO; Methyl Putrescine Oxidase
MeSH headings : Gene Expression Regulation, Plant; Plant Leaves / genetics; Plant Leaves / metabolism; RNA Interference; Tobacco / genetics; Tobacco / metabolism; Tobacco Products
TL;DR: Tobacco genotypes expressing an RNAi construct designed to reduce expression of the Methyl Putrescine Oxidase (MPO) gene family associated with nicotine biosynthesis are produced and evaluated under field conditions. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: July 11, 2022

2022 journal article

Editing of A622 genes results in ultra-low nicotine whole tobacco plants at the expense of dramatically reduced growth and development

MOLECULAR BREEDING, 42(4).

By: N. Burner  n, S. Kernodle n, T. Steede n & R. Lewis n 

author keywords: Tobacco; Nicotiana tabacum; Nicotine; Alkaloids; Gene editing; A622
TL;DR: A622 knockout lines exhibited lower nicotine levels than previously created genotypes with deleterious mutations in BBL genes also associated with one of the latter steps in tobacco alkaloid biosynthesis. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: April 18, 2022

2020 journal article

Genetic and Agronomic Analysis of Tobacco Genotypes Exhibiting Reduced Nicotine Accumulation Due to Induced Mutations in Berberine Bridge Like (BBL) Genes

FRONTIERS IN PLANT SCIENCE, 11.

By: R. Lewis n , K. Drake-Stowe n, C. Heim n, T. Steede n, W. Smith n & R. Dewey n

author keywords: tobacco; Nicotiana tabacum; nicotine; alkaloids; gene editing; regulation
TL;DR: Genetic methodologies for reducing nicotine accumulation in the tobacco plant (Nicotiana tabacum L.) are of interest because of potential future regulations that could mandate lowering of this alkaloid in conventional cigarettes. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: May 18, 2020

2018 journal article

Characterization of Nicotiana tabacum genotypes possessing deletion mutations that affect potyvirus resistance and the production of trichome exudates

BMC GENOMICS, 19.

By: K. Dluge n, Z. Song *, B. Wang *, W. Steede n, B. Xiao*, Y. Liu *, R. Dewey n

author keywords: Nicotiana tabacum; VAM; Va; Trichome exudate
MeSH headings : Chromosomes, Human, Pair 21 / genetics; Exudates and Transudates; Gene Expression Regulation, Plant / genetics; Genotype; Humans; Plant Proteins / genetics; Plants, Genetically Modified / genetics; Potyvirus / genetics; Tobacco / genetics; Trichomes / genetics
TL;DR: The feasibility of using RNA-seq and emerging whole genome sequence resources in tobacco to characterize the VAM and va deletion mutants is demonstrated and the discovery of genes underlying some of the phenotypic traits associated with these historically important loci are led to. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2017 journal article

The Tobacco Trichome Exudate Z-abienol and Its Relationship With Plant Resistance to Phytophthora nicotianae

PLANT DISEASE, 101(7), 1214–1221.

By: W. Steede n, J. Ma, D. Eickholt n, K. Drake-Stowe n, S. Kernodle n, H. Shew  n, D. Danehower n, R. Lewis n 

TL;DR: Results suggest that, although Z-abienol may affect P. nicotianae when applied at high concentrations in in vitro assays, the compound has little effect on black shank disease development under natural field conditions. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 journal article

Transgenic and Mutation-Based Suppression of a Berberine Bridge Enzyme-Like (BBL) Gene Family Reduces Alkaloid Content in Field-Grown Tobacco

PLOS ONE, 10(2).

By: R. Lewis n , H. Lopez n, S. Bowen n, K. Andres n, W. Steede n & R. Dewey n

MeSH headings : Alkaloids / biosynthesis; Alkaloids / chemistry; Gene Expression Regulation, Plant; Mutation; Plant Proteins / antagonists & inhibitors; Plant Proteins / genetics; Plant Proteins / metabolism; Plants, Genetically Modified / genetics; Plants, Genetically Modified / metabolism; RNA Interference; Tobacco / genetics; Tobacco / growth & development; Tobacco / metabolism
TL;DR: Transgenic doubled haploid lines of tobacco cultivar K326 carrying an RNAi construct designed to reduce expression of the BBL gene family were produced, indicating that a range of alkaloid phenotypes could be produced, with the triple homozygous knockout genotype exhibiting greater than a 13-fold reduction in percent total alkaloids. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2009 journal article

Analysis of a Nicotiana tabacum L. Genomic Region Controlling Two Leaf Surface Chemistry Traits

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 58(1), 294–300.

By: V. Vontimitta n, D. Danehower n, T. Steede n, H. Moon n & R. Lewis n 

author keywords: Tobacco; leaf surface chemistry; cis-abienol; sucrose esters; gene mapping; molecular markers
MeSH headings : Chromosome Mapping; Diterpenes / analysis; Diterpenes / metabolism; Esters / analysis; Esters / metabolism; Gene Expression Regulation, Plant; Genome, Plant; Microsatellite Repeats; Naphthols / analysis; Naphthols / metabolism; Plant Leaves / chemistry; Plant Leaves / genetics; Plant Leaves / metabolism; Plant Proteins / genetics; Plant Proteins / metabolism; Tobacco / chemistry; Tobacco / genetics; Tobacco / metabolism
TL;DR: A doubled haploid mapping population segregating for major genes affecting the ability to accumulate cis-abienol and sucrose esters was generated and genotyped with a large set of microsatellite markers, which should aid breeding efforts focused on modification of this aspect of tobacco cured leaf chemistry. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

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